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The map label for this gene is gsiB [H]

Identifier: 94311432

GI number: 94311432

Start: 2709915

End: 2711531

Strand: Direct

Name: gsiB [H]

Synonym: Rmet_2496

Alternate gene names: 94311432

Gene position: 2709915-2711531 (Clockwise)

Preceding gene: 94311430

Following gene: 94311435

Centisome position: 68.99

GC content: 63.64

Gene sequence:

>1617_bases
ATGGCCGAACACCGTACGAGGAGCCCACCGATGATCCGCCGCACGCTGCTGCAAGCATCCCTTTCGATTGCGTTGAGCGC
CACCGCGCTCACGCTTTTGCCCGCCCAGGCGGCTCATGCCGAAGACCTGCGCATCGGCCTGGCCGCCGACGTGACGTCCA
TGGACCCGCACTGGAACAACTCGGGCCCGAACAACGCCATCGCACTCCATATCTTCGAGTCGCTCGTATTCATGGACAAG
AACGCGCGCTACATTCCCGGCCTCGCGCTATCGTGGAAGCCACTCAACGCGATCACGTGGGAGATCAAGCTTCGCCCGAA
CGTGAAATGGCATGACGGCTCGCCGTTCACGAGCGAGGACGTCAAGGCGTCACTGGAGCGCCCCGAGAAGCTGACCAACG
CGCCGGGGTCGTTCACCAGCTACACCAAGCCGATCGCCCGCATCGACACGCCCGACCCGCTGACCGTGCGCCTGACCATG
AGCGTGCCGAATTATGCGAACCTGGCCAATGATCTGAACAGCGTGCCGATCATGCCGAAGAAGATTGCCGCGAATCTGAC
CCAGGCCGATTTCGATTCGGGCAAGGCGATGATCGGCACGGGTCCGTTCAAGTTCGTGCGCTTCGCGCGCGGCCAGGAAA
TCGTGATGACGAAGAATCCCGACTACTGGGGGCCAAAGCCCGAATGGGACCGCGCAATCTTCCGGATCCTGACCGACAAT
GGCGCGCGCTCGGCCGCCCTGCTTGCCGGCGATGTGGACGTGATCGAAAGCGTGCCATCCGCCGACGTGGCGAAGCTCAA
GCAGAACCCGAAGTTCAAGATCGAGCAGACGGTGTCGTGGCGCACGATCTTCTGGCAGATGGACCAGTCACGCGACAACC
CGCCGTTCGTCACGGACAAGGCAGGCAAGCCGCTCGGCAAGAATCCTTTCAAGGACGCGCGCGTGCGCGCGGCGATCAGC
AAGGCCCTCAACCGCGACGCCATTGTCAGCCGCATCATGGAAGGCTTGGCGGTGCCGGCCTCGTCGATCGTCTCGCCGCA
GATCTTCGGCCATCCTGGCACCAAGCCGGACGCCTATGACGTGGAAGGTGCGAAGAAGCTGCTGGCCGCCGCAGGCTATC
CGGACGGCTTCGGCCTCACGCTGCACGCCACGAACAATCGCTATCTGAATGACGCCCAGGTTGCGCAGGCCACGGCCGGG
ATGCTGACGCGTATCGGCATCCAGACCAAGGTTGAGACGCTGCCCGTTGCCACGTACTTCACGCGCGCGCGCCAGGGGGA
ATTTGCATTCGAGATGCTAGGCTGGGGATCGGCCGCTGCCGACGTGGCGCTACGCTCGATCACGGGCACCCCGAACCCGA
AGACCGGCTACGGCACGTGGAACTGGGGCAAGTACAGCAATCCGCAGCTTGATCAGCTGATCGAGAAATCGCTGACGACG
ATCTCCAGCGACAAGGCCCGCGAGGAGAATGCCCGCGCGGCGGCCAAGTTCGCGCTGGCCGACCACGCGATCATCCCGTC
GCACAGCCAGCTTGCAATGTGGGCGATGCGCAAGGGCCTGCGCTATGAGGCGCGCACCGACGAGTGGTCGCTGGCGCAGT
TCTTCCATAAGGAATGA

Upstream 100 bases:

>100_bases
GCTCATGGATATCGGACTGTGCCGACGAAATTGTGCGGGAATTGGCAAACATGGCCGCGATTGTACTGGGTCTGCAACGA
TGTATAGTTACCGGCACAAT

Downstream 100 bases:

>100_bases
TGCTGGCCTGGCTCCCCTCTCCCACCTGGTGGGAGAGGGGATAAAGCGCGACGTCAGATCGCCAGCCGCTCTTCCTGCCG
CGCATTCGCCGCGCGCAGCT

Product: putative extracellular solute-binding, family 5 ABC-type transporter protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MAEHRTRSPPMIRRTLLQASLSIALSATALTLLPAQAAHAEDLRIGLAADVTSMDPHWNNSGPNNAIALHIFESLVFMDK
NARYIPGLALSWKPLNAITWEIKLRPNVKWHDGSPFTSEDVKASLERPEKLTNAPGSFTSYTKPIARIDTPDPLTVRLTM
SVPNYANLANDLNSVPIMPKKIAANLTQADFDSGKAMIGTGPFKFVRFARGQEIVMTKNPDYWGPKPEWDRAIFRILTDN
GARSAALLAGDVDVIESVPSADVAKLKQNPKFKIEQTVSWRTIFWQMDQSRDNPPFVTDKAGKPLGKNPFKDARVRAAIS
KALNRDAIVSRIMEGLAVPASSIVSPQIFGHPGTKPDAYDVEGAKKLLAAAGYPDGFGLTLHATNNRYLNDAQVAQATAG
MLTRIGIQTKVETLPVATYFTRARQGEFAFEMLGWGSAAADVALRSITGTPNPKTGYGTWNWGKYSNPQLDQLIEKSLTT
ISSDKAREENARAAAKFALADHAIIPSHSQLAMWAMRKGLRYEARTDEWSLAQFFHKE

Sequences:

>Translated_538_residues
MAEHRTRSPPMIRRTLLQASLSIALSATALTLLPAQAAHAEDLRIGLAADVTSMDPHWNNSGPNNAIALHIFESLVFMDK
NARYIPGLALSWKPLNAITWEIKLRPNVKWHDGSPFTSEDVKASLERPEKLTNAPGSFTSYTKPIARIDTPDPLTVRLTM
SVPNYANLANDLNSVPIMPKKIAANLTQADFDSGKAMIGTGPFKFVRFARGQEIVMTKNPDYWGPKPEWDRAIFRILTDN
GARSAALLAGDVDVIESVPSADVAKLKQNPKFKIEQTVSWRTIFWQMDQSRDNPPFVTDKAGKPLGKNPFKDARVRAAIS
KALNRDAIVSRIMEGLAVPASSIVSPQIFGHPGTKPDAYDVEGAKKLLAAAGYPDGFGLTLHATNNRYLNDAQVAQATAG
MLTRIGIQTKVETLPVATYFTRARQGEFAFEMLGWGSAAADVALRSITGTPNPKTGYGTWNWGKYSNPQLDQLIEKSLTT
ISSDKAREENARAAAKFALADHAIIPSHSQLAMWAMRKGLRYEARTDEWSLAQFFHKE
>Mature_537_residues
AEHRTRSPPMIRRTLLQASLSIALSATALTLLPAQAAHAEDLRIGLAADVTSMDPHWNNSGPNNAIALHIFESLVFMDKN
ARYIPGLALSWKPLNAITWEIKLRPNVKWHDGSPFTSEDVKASLERPEKLTNAPGSFTSYTKPIARIDTPDPLTVRLTMS
VPNYANLANDLNSVPIMPKKIAANLTQADFDSGKAMIGTGPFKFVRFARGQEIVMTKNPDYWGPKPEWDRAIFRILTDNG
ARSAALLAGDVDVIESVPSADVAKLKQNPKFKIEQTVSWRTIFWQMDQSRDNPPFVTDKAGKPLGKNPFKDARVRAAISK
ALNRDAIVSRIMEGLAVPASSIVSPQIFGHPGTKPDAYDVEGAKKLLAAAGYPDGFGLTLHATNNRYLNDAQVAQATAGM
LTRIGIQTKVETLPVATYFTRARQGEFAFEMLGWGSAAADVALRSITGTPNPKTGYGTWNWGKYSNPQLDQLIEKSLTTI
SSDKAREENARAAAKFALADHAIIPSHSQLAMWAMRKGLRYEARTDEWSLAQFFHKE

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=464, Percent_Identity=28.6637931034483, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1789966, Length=519, Percent_Identity=26.2042389210019, Blast_Score=142, Evalue=7e-35,
Organism=Escherichia coli, GI1787762, Length=522, Percent_Identity=27.7777777777778, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1787495, Length=532, Percent_Identity=24.812030075188, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI87081878, Length=520, Percent_Identity=24.4230769230769, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789887, Length=444, Percent_Identity=23.8738738738739, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789397, Length=496, Percent_Identity=22.7822580645161, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1787551, Length=556, Percent_Identity=23.2014388489209, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI87082063, Length=498, Percent_Identity=20.6827309236948, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59162; Mature: 59031

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEHRTRSPPMIRRTLLQASLSIALSATALTLLPAQAAHAEDLRIGLAADVTSMDPHWNN
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEEEECCCCCCCCCCC
SGPNNAIALHIFESLVFMDKNARYIPGLALSWKPLNAITWEIKLRPNVKWHDGSPFTSED
CCCCCEEEHHHHHHHHHCCCCCCCCCCEEECCCCCCEEEEEEEECCCEEECCCCCCCHHH
VKASLERPEKLTNAPGSFTSYTKPIARIDTPDPLTVRLTMSVPNYANLANDLNSVPIMPK
HHHHHHCCHHHHCCCCCCCHHCCCHHCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCHH
KIAANLTQADFDSGKAMIGTGPFKFVRFARGQEIVMTKNPDYWGPKPEWDRAIFRILTDN
HHHCCCHHHHCCCCCEEEECCHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCC
GARSAALLAGDVDVIESVPSADVAKLKQNPKFKIEQTVSWRTIFWQMDQSRDNPPFVTDK
CCCCEEEEECCHHHHHCCCCHHHHHHHCCCCEEEEECCEEEEEEEEECCCCCCCCEEECC
AGKPLGKNPFKDARVRAAISKALNRDAIVSRIMEGLAVPASSIVSPQIFGHPGTKPDAYD
CCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHCCCCEECCCCCCCCCCC
VEGAKKLLAAAGYPDGFGLTLHATNNRYLNDAQVAQATAGMLTRIGIQTKVETLPVATYF
CHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEHHHEEHHHHH
TRARQGEFAFEMLGWGSAAADVALRSITGTPNPKTGYGTWNWGKYSNPQLDQLIEKSLTT
HHHCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ISSDKAREENARAAAKFALADHAIIPSHSQLAMWAMRKGLRYEARTDEWSLAQFFHKE
HCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
AEHRTRSPPMIRRTLLQASLSIALSATALTLLPAQAAHAEDLRIGLAADVTSMDPHWNN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEEEECCCCCCCCCCC
SGPNNAIALHIFESLVFMDKNARYIPGLALSWKPLNAITWEIKLRPNVKWHDGSPFTSED
CCCCCEEEHHHHHHHHHCCCCCCCCCCEEECCCCCCEEEEEEEECCCEEECCCCCCCHHH
VKASLERPEKLTNAPGSFTSYTKPIARIDTPDPLTVRLTMSVPNYANLANDLNSVPIMPK
HHHHHHCCHHHHCCCCCCCHHCCCHHCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCHH
KIAANLTQADFDSGKAMIGTGPFKFVRFARGQEIVMTKNPDYWGPKPEWDRAIFRILTDN
HHHCCCHHHHCCCCCEEEECCHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCC
GARSAALLAGDVDVIESVPSADVAKLKQNPKFKIEQTVSWRTIFWQMDQSRDNPPFVTDK
CCCCEEEEECCHHHHHCCCCHHHHHHHCCCCEEEEECCEEEEEEEEECCCCCCCCEEECC
AGKPLGKNPFKDARVRAAISKALNRDAIVSRIMEGLAVPASSIVSPQIFGHPGTKPDAYD
CCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHCCCCEECCCCCCCCCCC
VEGAKKLLAAAGYPDGFGLTLHATNNRYLNDAQVAQATAGMLTRIGIQTKVETLPVATYF
CHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEHHHEEHHHHH
TRARQGEFAFEMLGWGSAAADVALRSITGTPNPKTGYGTWNWGKYSNPQLDQLIEKSLTT
HHHCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ISSDKAREENARAAAKFALADHAIIPSHSQLAMWAMRKGLRYEARTDEWSLAQFFHKE
HCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA