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The map label for this gene is tiaE [H]

Identifier: 94311382

GI number: 94311382

Start: 2657397

End: 2658401

Strand: Reverse

Name: tiaE [H]

Synonym: Rmet_2446

Alternate gene names: 94311382

Gene position: 2658401-2657397 (Counterclockwise)

Preceding gene: 94311383

Following gene: 94311381

Centisome position: 67.68

GC content: 63.68

Gene sequence:

>1005_bases
ATGACTGATATGAAGCCCTCCATTCTGGTCACCCGCGCAATCTTCCCCGACGTCATCGAACGGCTCGCGCAGTACTTTGA
CGTCGATGACAACCAGGAGGATCTGGTGCTCGACAGCGGCGCACTGCGAGAACGACTGGCGGGCAAGGTGGGTGTTCTGG
CCAACGCTGCGGACCGGATCGATGGCGAACTGGTGGGGGCTTTGCCGCAGTTGCGTGCCGTATGCAACATGGCGGTCGGA
TACAACAACCTCGATCTGCCTGCGCTGACTGCGGCCGGCATCGTCGCGACCAACACGCCTGACGTGCTGACGGAAACCAC
CGCGGACTTCGGCTGGGCTCTGCTGATGGCAACGGCGCGTCGCGTGACCGAAGGGGAGCACTATCTTCGGGCTGGCAAAT
GGGAGCGCTGGAGCTACGACATGCTGGTCGGCATGGACGTCCAAGGCAGCACGCTGGGTATCCTCGGCATGGGGCGTATC
GGCCAGGGGCTGGCGCGACGCGCGGCAGGCTTCGACATGCAGGTGCTTTATCACAACCGGAGCCAGTTGCCGGACGATGT
CGAGCGTTCGCTCAATGCGCGATACGTGAGCAAGGCGGAACTGTTGCAGCAGTCCGACCACCTCATCCTGGTGCTGCCGT
ATAGCGTCGAAAGCCATCACGCCATTGGCGCAGCTGAAATCGCGCAGATGAAGCCGACTGCGACGCTGATCAATCTCGCG
CGCGGTGGCATCGTCGACGACGCCGCGCTTGCGGCGGCACTGCGCGCACGCAAGATCTTTGCAGCGGGGCTTGATGTGTT
CGAGGGCGAGCCGAGCGTGCATCCGGATCTGTTGACCGTGCCGAACGTCGTGCTGACGCCCCATATTGCCAGCGCTTCCG
AGAAGACGCGCCGCGCCATGGCCAACCTGGCCGCGGACAATCTGATCGCCGCGCTGGACGTGGGTCCGCAGGCCGGGAAG
CCGCCAACTGTCGTCAATCCTGAAGTGATGGCGCGTCGTCGCTGA

Upstream 100 bases:

>100_bases
GCCGTGGCGTGCGGATGCCGAGCTTCTTCGGCTACATGGCCTGGTCCTGCACTGTGCTGCTGCCGCTGTTCCTGGTGATG
ACGCTCCTCTTTTTCCACGT

Downstream 100 bases:

>100_bases
TTGCTGGGCTGCCCGAGACATGTCACTGGTTTCCATCCTGACCCTCGCGACCGCCGTGGTGGCGGTACTGCTGCTACTGG
TGCTGGTATGGCGCACGCAG

Product: 2-keto-D-gluconate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MTDMKPSILVTRAIFPDVIERLAQYFDVDDNQEDLVLDSGALRERLAGKVGVLANAADRIDGELVGALPQLRAVCNMAVG
YNNLDLPALTAAGIVATNTPDVLTETTADFGWALLMATARRVTEGEHYLRAGKWERWSYDMLVGMDVQGSTLGILGMGRI
GQGLARRAAGFDMQVLYHNRSQLPDDVERSLNARYVSKAELLQQSDHLILVLPYSVESHHAIGAAEIAQMKPTATLINLA
RGGIVDDAALAAALRARKIFAAGLDVFEGEPSVHPDLLTVPNVVLTPHIASASEKTRRAMANLAADNLIAALDVGPQAGK
PPTVVNPEVMARRR

Sequences:

>Translated_334_residues
MTDMKPSILVTRAIFPDVIERLAQYFDVDDNQEDLVLDSGALRERLAGKVGVLANAADRIDGELVGALPQLRAVCNMAVG
YNNLDLPALTAAGIVATNTPDVLTETTADFGWALLMATARRVTEGEHYLRAGKWERWSYDMLVGMDVQGSTLGILGMGRI
GQGLARRAAGFDMQVLYHNRSQLPDDVERSLNARYVSKAELLQQSDHLILVLPYSVESHHAIGAAEIAQMKPTATLINLA
RGGIVDDAALAAALRARKIFAAGLDVFEGEPSVHPDLLTVPNVVLTPHIASASEKTRRAMANLAADNLIAALDVGPQAGK
PPTVVNPEVMARRR
>Mature_333_residues
TDMKPSILVTRAIFPDVIERLAQYFDVDDNQEDLVLDSGALRERLAGKVGVLANAADRIDGELVGALPQLRAVCNMAVGY
NNLDLPALTAAGIVATNTPDVLTETTADFGWALLMATARRVTEGEHYLRAGKWERWSYDMLVGMDVQGSTLGILGMGRIG
QGLARRAAGFDMQVLYHNRSQLPDDVERSLNARYVSKAELLQQSDHLILVLPYSVESHHAIGAAEIAQMKPTATLINLAR
GGIVDDAALAAALRARKIFAAGLDVFEGEPSVHPDLLTVPNVVLTPHIASASEKTRRAMANLAADNLIAALDVGPQAGKP
PTVVNPEVMARRR

Specific function: Unknown

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GyaR subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=323, Percent_Identity=36.8421052631579, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI145580578, Length=249, Percent_Identity=36.144578313253, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI4557499, Length=249, Percent_Identity=36.144578313253, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI61743967, Length=249, Percent_Identity=35.7429718875502, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI4557497, Length=249, Percent_Identity=35.7429718875502, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI23308577, Length=255, Percent_Identity=30.9803921568627, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI145580575, Length=249, Percent_Identity=36.144578313253, Blast_Score=126, Evalue=3e-29,
Organism=Escherichia coli, GI87082289, Length=331, Percent_Identity=43.8066465256798, Blast_Score=239, Evalue=1e-64,
Organism=Escherichia coli, GI1789279, Length=322, Percent_Identity=28.8819875776398, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI1787645, Length=248, Percent_Identity=27.8225806451613, Blast_Score=96, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17532191, Length=259, Percent_Identity=30.8880308880309, Blast_Score=134, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI25147481, Length=273, Percent_Identity=25.6410256410256, Blast_Score=88, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6324055, Length=253, Percent_Identity=37.1541501976285, Blast_Score=165, Evalue=8e-42,
Organism=Saccharomyces cerevisiae, GI6320925, Length=293, Percent_Identity=29.6928327645051, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6322116, Length=333, Percent_Identity=27.9279279279279, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6325144, Length=147, Percent_Identity=32.6530612244898, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321253, Length=167, Percent_Identity=29.3413173652695, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324964, Length=279, Percent_Identity=24.0143369175627, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28574286, Length=281, Percent_Identity=40.2135231316726, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI45552429, Length=288, Percent_Identity=37.8472222222222, Blast_Score=190, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24585514, Length=288, Percent_Identity=37.8472222222222, Blast_Score=190, Evalue=1e-48,
Organism=Drosophila melanogaster, GI28574282, Length=288, Percent_Identity=37.8472222222222, Blast_Score=190, Evalue=1e-48,
Organism=Drosophila melanogaster, GI45551003, Length=288, Percent_Identity=37.8472222222222, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI28574284, Length=288, Percent_Identity=37.8472222222222, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI28571528, Length=313, Percent_Identity=37.6996805111821, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24585516, Length=257, Percent_Identity=33.0739299610895, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24646446, Length=269, Percent_Identity=34.5724907063197, Blast_Score=130, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24646448, Length=269, Percent_Identity=34.5724907063197, Blast_Score=130, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24646452, Length=269, Percent_Identity=34.5724907063197, Blast_Score=130, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24646450, Length=269, Percent_Identity=34.5724907063197, Blast_Score=130, Evalue=9e-31,
Organism=Drosophila melanogaster, GI62472511, Length=249, Percent_Identity=34.9397590361446, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI19921140, Length=271, Percent_Identity=28.4132841328413, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.26 [H]

Molecular weight: Translated: 35945; Mature: 35813

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDMKPSILVTRAIFPDVIERLAQYFDVDDNQEDLVLDSGALRERLAGKVGVLANAADRI
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHCCHHHHHHHHHHHH
DGELVGALPQLRAVCNMAVGYNNLDLPALTAAGIVATNTPDVLTETTADFGWALLMATAR
CHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHH
RVTEGEHYLRAGKWERWSYDMLVGMDVQGSTLGILGMGRIGQGLARRAAGFDMQVLYHNR
HHHCCHHHHHCCCCCCCCEEEEEEECCCCCEEEEEECCHHHHHHHHHHCCCCEEEEECCC
SQLPDDVERSLNARYVSKAELLQQSDHLILVLPYSVESHHAIGAAEIAQMKPTATLINLA
CCCCHHHHHHHCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHCCCHHHHHHHH
RGGIVDDAALAAALRARKIFAAGLDVFEGEPSVHPDLLTVPNVVLTPHIASASEKTRRAM
CCCCCHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCEECCCEEECCCCCCHHHHHHHHH
ANLAADNLIAALDVGPQAGKPPTVVNPEVMARRR
HHHHHHHEEEEEECCCCCCCCCCCCCHHHHHCCC
>Mature Secondary Structure 
TDMKPSILVTRAIFPDVIERLAQYFDVDDNQEDLVLDSGALRERLAGKVGVLANAADRI
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHCCHHHHHHHHHHHH
DGELVGALPQLRAVCNMAVGYNNLDLPALTAAGIVATNTPDVLTETTADFGWALLMATAR
CHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHH
RVTEGEHYLRAGKWERWSYDMLVGMDVQGSTLGILGMGRIGQGLARRAAGFDMQVLYHNR
HHHCCHHHHHCCCCCCCCEEEEEEECCCCCEEEEEECCHHHHHHHHHHCCCCEEEEECCC
SQLPDDVERSLNARYVSKAELLQQSDHLILVLPYSVESHHAIGAAEIAQMKPTATLINLA
CCCCHHHHHHHCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHCCCHHHHHHHH
RGGIVDDAALAAALRARKIFAAGLDVFEGEPSVHPDLLTVPNVVLTPHIASASEKTRRAM
CCCCCHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCEECCCEEECCCCCCHHHHHHHHH
ANLAADNLIAALDVGPQAGKPPTVVNPEVMARRR
HHHHHHHEEEEEECCCCCCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA