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The map label for this gene is moaA [H]

Identifier: 94311377

GI number: 94311377

Start: 2652199

End: 2653386

Strand: Direct

Name: moaA [H]

Synonym: Rmet_2441

Alternate gene names: 94311377

Gene position: 2652199-2653386 (Clockwise)

Preceding gene: 94311376

Following gene: 94311378

Centisome position: 67.52

GC content: 63.55

Gene sequence:

>1188_bases
ATGGCTGACAGAAAGGCCTCCCTGGCGCCCGCGCGCCGAAGAGCCTCCGTCCTCACCCTGGCCATGACCGATACCGTCAT
CCCCATTCTCGACCTGCGCGACCATCGATACCGTTCGATGGTGCCGAGCATTCCTTCGCACCTGGTTGCGCCGAACGGAC
TCGTCGCCGATACACGCGGCCGGCCGCTGCATGACCTGCGTATCTCGGTCACTGACCGCTGCAACTTCCGCTGCGTGTAC
TGCATGCCGAAGGAAGTGTTCGACAAGGACTACACGTTCCTGCCCCACTCCGAGTTGCTGAGCTTCGAAGAGATCGAGCG
CACCGCGCGCATTTTCGTCTCGCAGGGTGTGGAGAAGATTCGTTTGACCGGCGGCGAACCGCTACTGCGCAAGAACATCG
AGCGACTCGTGGAAATGCTCGCGCGCATCGAAACCGTATCGGGCAAGCCGCTGGACCTGACGCTGACCACCAACGCATCA
CTGCTCGCACGCAAGGCGCAGTCATTGCGCGACGCCGGACTGTCCCGCATCAGCGTGAGCCTTGATGCCATTGACGACGC
GATCTTCCGCAAGATGAACGACGTCGACTATGCCGTGCGCGACGTGCTCCACGGCATCGAAGTGGCGCAAGCCGTCGGGC
TGGCGCCGATCAAGGTCAACATGGTGGTCAAGCGTGGCACCAACGACGGCGAAATTGTGCCGTTGGCGCGCCACTTCCGC
GGCAGCGGCATCATCATGCGCTTCATCGAGTTCATGGACGTGGGCGCCAGCAACCACTGGCAGATGGGCGAAGTTCTGCC
ATCGGCCGAAGTCGTGCGCCGGATCGACGAAGTGTTTCCGCTGGACGCTGTGGCCGCCAATTACTCGGGCGAGACCGCAG
AGCGTTGGCGCTACCGGGATGGCGGCGGCGAGATCGGCGTGATTTCGAGCGTCACGCAGGCCTTCTGCGGAGACTGCAGC
CGCATCCGGCTGTCGACCGAAGGTCGGCTCTATCTGTGCCTGTTCGCCACCGAGGGCTACGACCTGCGCGCCCTGCTGCG
CGGTGGGCACAGCGACCTGGAGATCGCCAACGCAATCGCCCAGGTCTGGCAAGGCCGCACCGACAACTATTCCGAACAGC
GCAGCGATCCGGCAATTCGCGCGGCACGCGCCGAGCGCAAGATCGAGATGTCCTATATCGGCGGGTGA

Upstream 100 bases:

>100_bases
GACTTGCTGCAATGCCGCAAGCTGTATTGCAGCTGCCTCGGGCAAACTGTGACCCTATGGCAATAATGTCATCCACGATG
CCCGATGCCGCTCGGCTAGA

Downstream 100 bases:

>100_bases
TGCCCTTCCGGGCGCCGCGCCCCAATCCTCGAGATCCATGATTTCACGCGACGACATAACCGGCCTGATCCTGGCCGGCG
GCCGAGGCAGCCGCATGGGC

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MADRKASLAPARRRASVLTLAMTDTVIPILDLRDHRYRSMVPSIPSHLVAPNGLVADTRGRPLHDLRISVTDRCNFRCVY
CMPKEVFDKDYTFLPHSELLSFEEIERTARIFVSQGVEKIRLTGGEPLLRKNIERLVEMLARIETVSGKPLDLTLTTNAS
LLARKAQSLRDAGLSRISVSLDAIDDAIFRKMNDVDYAVRDVLHGIEVAQAVGLAPIKVNMVVKRGTNDGEIVPLARHFR
GSGIIMRFIEFMDVGASNHWQMGEVLPSAEVVRRIDEVFPLDAVAANYSGETAERWRYRDGGGEIGVISSVTQAFCGDCS
RIRLSTEGRLYLCLFATEGYDLRALLRGGHSDLEIANAIAQVWQGRTDNYSEQRSDPAIRAARAERKIEMSYIGG

Sequences:

>Translated_395_residues
MADRKASLAPARRRASVLTLAMTDTVIPILDLRDHRYRSMVPSIPSHLVAPNGLVADTRGRPLHDLRISVTDRCNFRCVY
CMPKEVFDKDYTFLPHSELLSFEEIERTARIFVSQGVEKIRLTGGEPLLRKNIERLVEMLARIETVSGKPLDLTLTTNAS
LLARKAQSLRDAGLSRISVSLDAIDDAIFRKMNDVDYAVRDVLHGIEVAQAVGLAPIKVNMVVKRGTNDGEIVPLARHFR
GSGIIMRFIEFMDVGASNHWQMGEVLPSAEVVRRIDEVFPLDAVAANYSGETAERWRYRDGGGEIGVISSVTQAFCGDCS
RIRLSTEGRLYLCLFATEGYDLRALLRGGHSDLEIANAIAQVWQGRTDNYSEQRSDPAIRAARAERKIEMSYIGG
>Mature_394_residues
ADRKASLAPARRRASVLTLAMTDTVIPILDLRDHRYRSMVPSIPSHLVAPNGLVADTRGRPLHDLRISVTDRCNFRCVYC
MPKEVFDKDYTFLPHSELLSFEEIERTARIFVSQGVEKIRLTGGEPLLRKNIERLVEMLARIETVSGKPLDLTLTTNASL
LARKAQSLRDAGLSRISVSLDAIDDAIFRKMNDVDYAVRDVLHGIEVAQAVGLAPIKVNMVVKRGTNDGEIVPLARHFRG
SGIIMRFIEFMDVGASNHWQMGEVLPSAEVVRRIDEVFPLDAVAANYSGETAERWRYRDGGGEIGVISSVTQAFCGDCSR
IRLSTEGRLYLCLFATEGYDLRALLRGGHSDLEIANAIAQVWQGRTDNYSEQRSDPAIRAARAERKIEMSYIGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z [H]

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family [H]

Homologues:

Organism=Homo sapiens, GI116734668, Length=319, Percent_Identity=35.7366771159875, Blast_Score=191, Evalue=7e-49,
Organism=Homo sapiens, GI22027536, Length=319, Percent_Identity=35.7366771159875, Blast_Score=191, Evalue=1e-48,
Organism=Escherichia coli, GI1786999, Length=348, Percent_Identity=31.6091954022989, Blast_Score=159, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17561210, Length=296, Percent_Identity=33.445945945946, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI32566331, Length=304, Percent_Identity=33.2236842105263, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI32566329, Length=304, Percent_Identity=33.2236842105263, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI28574488, Length=310, Percent_Identity=35.8064516129032, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI28574490, Length=310, Percent_Identity=35.8064516129032, Blast_Score=174, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 44003; Mature: 43872

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRKASLAPARRRASVLTLAMTDTVIPILDLRDHRYRSMVPSIPSHLVAPNGLVADTRG
CCCCCCCCCHHHHHHHEEEEEECCCCEEEECCCCCHHHHHCCCCHHHHCCCCCEEECCCC
RPLHDLRISVTDRCNFRCVYCMPKEVFDKDYTFLPHSELLSFEEIERTARIFVSQGVEKI
CCCEEEEEEEECCCCEEEEEECCHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCEE
RLTGGEPLLRKNIERLVEMLARIETVSGKPLDLTLTTNASLLARKAQSLRDAGLSRISVS
EECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEE
LDAIDDAIFRKMNDVDYAVRDVLHGIEVAQAVGLAPIKVNMVVKRGTNDGEIVPLARHFR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCEEEHHHHHC
GSGIIMRFIEFMDVGASNHWQMGEVLPSAEVVRRIDEVFPLDAVAANYSGETAERWRYRD
CCHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHCCHHHHHCCCCCCCHHHCEEEC
GGGEIGVISSVTQAFCGDCSRIRLSTEGRLYLCLFATEGYDLRALLRGGHSDLEIANAIA
CCCCEEHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCHHHHHHCCCCHHHHHHHHH
QVWQGRTDNYSEQRSDPAIRAARAERKIEMSYIGG
HHHCCCCCCCHHHCCCHHHHHHHHHCEEEEEECCC
>Mature Secondary Structure 
ADRKASLAPARRRASVLTLAMTDTVIPILDLRDHRYRSMVPSIPSHLVAPNGLVADTRG
CCCCCCCCHHHHHHHEEEEEECCCCEEEECCCCCHHHHHCCCCHHHHCCCCCEEECCCC
RPLHDLRISVTDRCNFRCVYCMPKEVFDKDYTFLPHSELLSFEEIERTARIFVSQGVEKI
CCCEEEEEEEECCCCEEEEEECCHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCEE
RLTGGEPLLRKNIERLVEMLARIETVSGKPLDLTLTTNASLLARKAQSLRDAGLSRISVS
EECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEE
LDAIDDAIFRKMNDVDYAVRDVLHGIEVAQAVGLAPIKVNMVVKRGTNDGEIVPLARHFR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCEEEHHHHHC
GSGIIMRFIEFMDVGASNHWQMGEVLPSAEVVRRIDEVFPLDAVAANYSGETAERWRYRD
CCHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHCCHHHHHCCCCCCCHHHCEEEC
GGGEIGVISSVTQAFCGDCSRIRLSTEGRLYLCLFATEGYDLRALLRGGHSDLEIANAIA
CCCCEEHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCHHHHHHCCCCHHHHHHHHH
QVWQGRTDNYSEQRSDPAIRAARAERKIEMSYIGG
HHHCCCCCCCHHHCCCHHHHHHHHHCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]