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The map label for this gene is paaF [C]

Identifier: 94311302

GI number: 94311302

Start: 2578766

End: 2579635

Strand: Direct

Name: paaF [C]

Synonym: Rmet_2366

Alternate gene names: 94311302

Gene position: 2578766-2579635 (Clockwise)

Preceding gene: 94311300

Following gene: 94311306

Centisome position: 65.65

GC content: 64.48

Gene sequence:

>870_bases
ATGCCTGCCGGACCGAGCATATCGACCTGCCCGCCGCAGACCACCATGTTCATGACCCACTGCAAGGAGAACTTCATGTC
CGAGCCCATTCTGCTGACCTCCGAACAAGACAACATCGGCATCGTCACGCTCAACATGGCGCACAAGCGCAACGCGCTCA
ATGAGGAAGCGATCGCCGCCATCGACGCCTATTTCTCAGCGGTGCCCAGCCACATCCGGGCCATCCTCCTGACGGCCAAG
GGCGATCACTTCTGTGCCGGCCTGGACCTCAAGGAACATCACGACAAGGCACGCAGCGGCGTCGAGTTCATGCGCGTCTG
CCAGGGCTGGCACCGCGCGTTCGACAAGATCCAGCATGGCGGCATTCCCGTGGTCGCCTGCCTGCAGGGAGCGGTCGTGG
GCGGCGGGCTGGAACTGGCCAGCGCAGCCCACGTGCGCGTTGCCGACAGCCGCACCTTCTTCGCACTGCCCGAGGGCACC
CGAGGCATCTTCACGGGCGGCGGCGCCACGGTGCGCACTGCGCGGATCATCTCCGCCCCTCGCATGGTGGAGATGATGCT
CACCGGACGGGTCCTCGATGCGCGGGAGGGCCGCGAACTGGGTTTGGCCCACTACGTATGCGACATCGCGAAAGGCGACA
CGCCCGCACCCGAGTTCGCCCTGACCCTCGCAAAGAAGATCGCGGGCAATGCGCCCCTTTCAAACTACGCCATCGTCAGC
GCCATCAGCCGCATCGCCGACATGTCGGCCACGGACGGGCTGTTCACCGAGGGGCTGGTGATGGCCATGATCCAGCAGGG
TGACGATGTGCAGGAGCGCCTGGGCGATTTCGTGAACAAGAAGGCGCACAAGGTCCAACTGAATGCTTGA

Upstream 100 bases:

>100_bases
CAGTGCGCAAGAATTGGCAACATTGGCATAGATCCTCGGCCTTGAACGGATGGTCCGCTTCAGTGCGGGCCATGCCGTCA
CCGCAGCGGCGAGGCGGCCG

Downstream 100 bases:

>100_bases
TCTTCAAGACGCGCGCTACCGGCCTCGGCCAGCGATGCGCGACCGCGTCATGGCTGGGCTCAGGTCACTTGCAAGGACCC
CCTGGCGCCCATCAGCAACT

Product: putative enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MPAGPSISTCPPQTTMFMTHCKENFMSEPILLTSEQDNIGIVTLNMAHKRNALNEEAIAAIDAYFSAVPSHIRAILLTAK
GDHFCAGLDLKEHHDKARSGVEFMRVCQGWHRAFDKIQHGGIPVVACLQGAVVGGGLELASAAHVRVADSRTFFALPEGT
RGIFTGGGATVRTARIISAPRMVEMMLTGRVLDAREGRELGLAHYVCDIAKGDTPAPEFALTLAKKIAGNAPLSNYAIVS
AISRIADMSATDGLFTEGLVMAMIQQGDDVQERLGDFVNKKAHKVQLNA

Sequences:

>Translated_289_residues
MPAGPSISTCPPQTTMFMTHCKENFMSEPILLTSEQDNIGIVTLNMAHKRNALNEEAIAAIDAYFSAVPSHIRAILLTAK
GDHFCAGLDLKEHHDKARSGVEFMRVCQGWHRAFDKIQHGGIPVVACLQGAVVGGGLELASAAHVRVADSRTFFALPEGT
RGIFTGGGATVRTARIISAPRMVEMMLTGRVLDAREGRELGLAHYVCDIAKGDTPAPEFALTLAKKIAGNAPLSNYAIVS
AISRIADMSATDGLFTEGLVMAMIQQGDDVQERLGDFVNKKAHKVQLNA
>Mature_288_residues
PAGPSISTCPPQTTMFMTHCKENFMSEPILLTSEQDNIGIVTLNMAHKRNALNEEAIAAIDAYFSAVPSHIRAILLTAKG
DHFCAGLDLKEHHDKARSGVEFMRVCQGWHRAFDKIQHGGIPVVACLQGAVVGGGLELASAAHVRVADSRTFFALPEGTR
GIFTGGGATVRTARIISAPRMVEMMLTGRVLDAREGRELGLAHYVCDIAKGDTPAPEFALTLAKKIAGNAPLSNYAIVSA
ISRIADMSATDGLFTEGLVMAMIQQGDDVQERLGDFVNKKAHKVQLNA

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=250, Percent_Identity=27.6, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI20127408, Length=250, Percent_Identity=28, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI68989263, Length=189, Percent_Identity=29.1005291005291, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI4502327, Length=258, Percent_Identity=30.2325581395349, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI70995211, Length=278, Percent_Identity=25.1798561151079, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1787659, Length=260, Percent_Identity=30.7692307692308, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788682, Length=196, Percent_Identity=32.1428571428571, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI221142681, Length=179, Percent_Identity=30.1675977653631, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788597, Length=244, Percent_Identity=28.2786885245902, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17540714, Length=257, Percent_Identity=27.6264591439689, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25145438, Length=257, Percent_Identity=29.1828793774319, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17560910, Length=207, Percent_Identity=32.3671497584541, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=229, Percent_Identity=27.0742358078603, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17558304, Length=179, Percent_Identity=27.3743016759777, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17508951, Length=244, Percent_Identity=26.6393442622951, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI25144276, Length=244, Percent_Identity=26.6393442622951, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17508953, Length=244, Percent_Identity=26.6393442622951, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17554946, Length=249, Percent_Identity=26.5060240963855, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24653139, Length=254, Percent_Identity=27.9527559055118, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI20129971, Length=256, Percent_Identity=27.34375, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24653477, Length=256, Percent_Identity=27.34375, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI19921000, Length=163, Percent_Identity=33.7423312883436, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24583077, Length=163, Percent_Identity=33.7423312883436, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24583079, Length=163, Percent_Identity=33.7423312883436, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19920382, Length=174, Percent_Identity=28.735632183908, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28571730, Length=217, Percent_Identity=28.110599078341, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28571729, Length=217, Percent_Identity=28.110599078341, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 30966; Mature: 30835

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAGPSISTCPPQTTMFMTHCKENFMSEPILLTSEQDNIGIVTLNMAHKRNALNEEAIAA
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEECHHHHCCCCHHHHHH
IDAYFSAVPSHIRAILLTAKGDHFCAGLDLKEHHDKARSGVEFMRVCQGWHRAFDKIQHG
HHHHHHHHHHHHEEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GIPVVACLQGAVVGGGLELASAAHVRVADSRTFFALPEGTRGIFTGGGATVRTARIISAP
CCHHHHHHHCHHCCCCHHHHCCCEEEEECCCEEEECCCCCCEEEECCCCEEEHHHHHCCH
RMVEMMLTGRVLDAREGRELGLAHYVCDIAKGDTPAPEFALTLAKKIAGNAPLSNYAIVS
HHHHHHHHCCHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHH
AISRIADMSATDGLFTEGLVMAMIQQGDDVQERLGDFVNKKAHKVQLNA
HHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure 
PAGPSISTCPPQTTMFMTHCKENFMSEPILLTSEQDNIGIVTLNMAHKRNALNEEAIAA
CCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEECHHHHCCCCHHHHHH
IDAYFSAVPSHIRAILLTAKGDHFCAGLDLKEHHDKARSGVEFMRVCQGWHRAFDKIQHG
HHHHHHHHHHHHEEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GIPVVACLQGAVVGGGLELASAAHVRVADSRTFFALPEGTRGIFTGGGATVRTARIISAP
CCHHHHHHHCHHCCCCHHHHCCCEEEEECCCEEEECCCCCCEEEECCCCEEEHHHHHCCH
RMVEMMLTGRVLDAREGRELGLAHYVCDIAKGDTPAPEFALTLAKKIAGNAPLSNYAIVS
HHHHHHHHCCHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHH
AISRIADMSATDGLFTEGLVMAMIQQGDDVQERLGDFVNKKAHKVQLNA
HHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA