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The map label for this gene is topB2 [H]

Identifier: 94311236

GI number: 94311236

Start: 2518269

End: 2520299

Strand: Direct

Name: topB2 [H]

Synonym: Rmet_2298

Alternate gene names: 94311236

Gene position: 2518269-2520299 (Clockwise)

Preceding gene: 94311235

Following gene: 335055593

Centisome position: 64.11

GC content: 66.42

Gene sequence:

>2031_bases
ATGCGGCTGTTCTTGTGCGAGAAGCCCTCCCAGGGCAAAGACATTGGCCGGATTCTCGGCGCCACGCAGCGCGGTGAAGG
CTGCCTCAACGGTTCCGGCGTCACGGTCACCTGGTGCATCGGCCATCTCGTGGAGGCGGCGCCGCCCGAGGCCTACGACG
AGCAGCTCAAACGATGGTCCGTTGAGCAGTTGCCCATCATTCCCCAGCACTGGCGGGTCGAGGTCAAACCGAAGACCGCC
ACGCAATTCAAGGTCGTCAAGGCGCTCTTGGCGAAGGCGACTCACCTGGTTATCGCCACCGATGCCGACCGCGAGGGCGA
ATTGATCGCCCGCGAGATCGTGGAGCTTTGCGGCTACCGCGGCCCCATCGAACGCCTGTGGCTGTCGGCGCTCAACGATG
CGTCCATTCGGGCGGCACTGGGCAAGCTGCGGCCTTCGGCCGAGACGCTTTCGATGTACCACTCGGCGCTGGCGCGCTCC
CGTGCGGATTGGCTCGTGGGCATGAACCTGAGCCGGCTGTTCACGGTGCTGGGGCGACAGGCGGGTTACGACGGCGTGCT
GTCGGTCGGCCGCGTACAGACCCCGACGCTCAAGCTCGTTGTGGACCGCGACCGCGAGATCGCGCGCTTCGTGTCCGTAC
CATACTGGGCCATCGCCGTGTCCCTGTTCGCAGGCGGTTCGACTTTCGCCGCGCAATGGGTTCCACCCGATGCGTGCACC
GACGACGCAGGCCGCTGCCTGCGGCAGCCGGTCGCACAGCAGACCATGCAGCAGATCCGCGCTGCGGGCAGTGCCCACGT
CGTGTCGGTGGAGACTGAGCGTGTCCGCGAAGGCCCGCCGCTGCCGTTCGACCTGGGCACCTTGCAGGAAGTGTGTTCCA
AGCAGCTTGGGCTGGACGTGCAGGAAACCTTGGAGATTGCCCAAGCCCTGTACGAGACGCACAAGGCCACGACGTACCCC
CGCTCGGACTCCGGCTACCTGCCCGAGAGCATGTTTGCCGAAGTGCCCACCGTTCTCGACAGCCTGCTCAAGACCGATCC
CTCGCTGCGCTCGATCATGGGCCAGCTCGACCGCTCGCAGCGCTCGCGCGCCTGGAACGATGGCAAGGTCACGGCGCACC
ACGGCATCATCCCGACGCTCGAACCGGCGAAGCTCTCCGCCATGAGCGAGAAGGAACTGGCCGTGTACAGGCTCATCCGG
TCGCATTACCTGGCGCAGTTCCTCCCTCACCACGAGTTCGACCGCACTGTGGCCAAGTTTTCGTGCGGGGGGCAGAACCT
GGCGGCCACGGGCAAGCAGGTTGTCATCCCGGGTTGGCGCCAGGTGCTCGCCGAGCCGCAGGCCGAAGACGGTGATGGCG
AGGGCGATACTGCGGTCCGCGCCCAGGTGCTGCCCGCGCTGCCGAAACTGTATGAGGGCCTGGCATGCCAGGTGGCCGAC
GTCGATCTCAAGGCACTCAAGACGCTGCCGCCCAAACCGTACACGCAAGGCGAGTTGGTCAAGTCCATGAAAGGCGTCGC
CAAGCTGGTGTCCGATCCCCGCCTGAAGCAGAAGCTCAAGGATACGGTTGGCATCGGCACCGAAGCGACGCGGGCCAACA
TCATCGGCGGCCTGATCGCTCGCGGCTACCTCGTGAAGAAGGGGCGCGCCATCCGCGCCTCGGATGCGGCTTTCACTTTG
ATCGATGCCGTGCCTGCGGCGATTGCCGACCCTGGCACCACCGCCGTCTGGGAACAGGCGCTCGACATGATCGAGGCCGG
ACAGCTCACCCTGGACGTGTTCATCGGCAAGCAGGCCGCGTGGATTTCGCAGTTGATTGCGCAGTACGGCAGCGCCTCCC
TGTCCATCAAGGTTCCCCAAGGGCCGGCATGCCCGCAGTGCGGCGCACCCACGCGCCAGCGCAGCGGCAAGAGCGGCCCG
TTCTGGTCGTGCAGCCGCTACCCGGACTGCAAAGGCACGCTGCCAGTCGAATCCGGCAGCTCCAAGCGCGGCGCCTCGCG
CCCGCGCCGTAGCGGCCGCAAAGGCTCCTGA

Upstream 100 bases:

>100_bases
GGTCGCTGCCGCATGCGGCTTGTTTGCTGCTGCCAGGGGCTGCGCTCGGCATCCTCGATCCCAGCAACTCCATGAACAAC
AGGAATCGGGATGGACGGAT

Downstream 100 bases:

>100_bases
CCGCCCCCGTTCCCCGTGAGCCGTGCCCGCCTTCGGCGGCGTGGCCCCTGTCCCGCACTCCGCCGCATGCCCTGCGGGAC
GCCCAGCGCGCAACGCCTTC

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 676; Mature: 676

Protein sequence:

>676_residues
MRLFLCEKPSQGKDIGRILGATQRGEGCLNGSGVTVTWCIGHLVEAAPPEAYDEQLKRWSVEQLPIIPQHWRVEVKPKTA
TQFKVVKALLAKATHLVIATDADREGELIAREIVELCGYRGPIERLWLSALNDASIRAALGKLRPSAETLSMYHSALARS
RADWLVGMNLSRLFTVLGRQAGYDGVLSVGRVQTPTLKLVVDRDREIARFVSVPYWAIAVSLFAGGSTFAAQWVPPDACT
DDAGRCLRQPVAQQTMQQIRAAGSAHVVSVETERVREGPPLPFDLGTLQEVCSKQLGLDVQETLEIAQALYETHKATTYP
RSDSGYLPESMFAEVPTVLDSLLKTDPSLRSIMGQLDRSQRSRAWNDGKVTAHHGIIPTLEPAKLSAMSEKELAVYRLIR
SHYLAQFLPHHEFDRTVAKFSCGGQNLAATGKQVVIPGWRQVLAEPQAEDGDGEGDTAVRAQVLPALPKLYEGLACQVAD
VDLKALKTLPPKPYTQGELVKSMKGVAKLVSDPRLKQKLKDTVGIGTEATRANIIGGLIARGYLVKKGRAIRASDAAFTL
IDAVPAAIADPGTTAVWEQALDMIEAGQLTLDVFIGKQAAWISQLIAQYGSASLSIKVPQGPACPQCGAPTRQRSGKSGP
FWSCSRYPDCKGTLPVESGSSKRGASRPRRSGRKGS

Sequences:

>Translated_676_residues
MRLFLCEKPSQGKDIGRILGATQRGEGCLNGSGVTVTWCIGHLVEAAPPEAYDEQLKRWSVEQLPIIPQHWRVEVKPKTA
TQFKVVKALLAKATHLVIATDADREGELIAREIVELCGYRGPIERLWLSALNDASIRAALGKLRPSAETLSMYHSALARS
RADWLVGMNLSRLFTVLGRQAGYDGVLSVGRVQTPTLKLVVDRDREIARFVSVPYWAIAVSLFAGGSTFAAQWVPPDACT
DDAGRCLRQPVAQQTMQQIRAAGSAHVVSVETERVREGPPLPFDLGTLQEVCSKQLGLDVQETLEIAQALYETHKATTYP
RSDSGYLPESMFAEVPTVLDSLLKTDPSLRSIMGQLDRSQRSRAWNDGKVTAHHGIIPTLEPAKLSAMSEKELAVYRLIR
SHYLAQFLPHHEFDRTVAKFSCGGQNLAATGKQVVIPGWRQVLAEPQAEDGDGEGDTAVRAQVLPALPKLYEGLACQVAD
VDLKALKTLPPKPYTQGELVKSMKGVAKLVSDPRLKQKLKDTVGIGTEATRANIIGGLIARGYLVKKGRAIRASDAAFTL
IDAVPAAIADPGTTAVWEQALDMIEAGQLTLDVFIGKQAAWISQLIAQYGSASLSIKVPQGPACPQCGAPTRQRSGKSGP
FWSCSRYPDCKGTLPVESGSSKRGASRPRRSGRKGS
>Mature_676_residues
MRLFLCEKPSQGKDIGRILGATQRGEGCLNGSGVTVTWCIGHLVEAAPPEAYDEQLKRWSVEQLPIIPQHWRVEVKPKTA
TQFKVVKALLAKATHLVIATDADREGELIAREIVELCGYRGPIERLWLSALNDASIRAALGKLRPSAETLSMYHSALARS
RADWLVGMNLSRLFTVLGRQAGYDGVLSVGRVQTPTLKLVVDRDREIARFVSVPYWAIAVSLFAGGSTFAAQWVPPDACT
DDAGRCLRQPVAQQTMQQIRAAGSAHVVSVETERVREGPPLPFDLGTLQEVCSKQLGLDVQETLEIAQALYETHKATTYP
RSDSGYLPESMFAEVPTVLDSLLKTDPSLRSIMGQLDRSQRSRAWNDGKVTAHHGIIPTLEPAKLSAMSEKELAVYRLIR
SHYLAQFLPHHEFDRTVAKFSCGGQNLAATGKQVVIPGWRQVLAEPQAEDGDGEGDTAVRAQVLPALPKLYEGLACQVAD
VDLKALKTLPPKPYTQGELVKSMKGVAKLVSDPRLKQKLKDTVGIGTEATRANIIGGLIARGYLVKKGRAIRASDAAFTL
IDAVPAAIADPGTTAVWEQALDMIEAGQLTLDVFIGKQAAWISQLIAQYGSASLSIKVPQGPACPQCGAPTRQRSGKSGP
FWSCSRYPDCKGTLPVESGSSKRGASRPRRSGRKGS

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=713, Percent_Identity=23.7026647966339, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI4507635, Length=681, Percent_Identity=22.0264317180617, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1788061, Length=657, Percent_Identity=38.6605783866058, Blast_Score=427, Evalue=1e-120,
Organism=Escherichia coli, GI1787529, Length=694, Percent_Identity=25.5043227665706, Blast_Score=136, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI32563869, Length=590, Percent_Identity=24.406779661017, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17555378, Length=658, Percent_Identity=23.7082066869301, Blast_Score=112, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6323263, Length=505, Percent_Identity=27.5247524752475, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24585251, Length=734, Percent_Identity=25.0681198910082, Blast_Score=124, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24640096, Length=544, Percent_Identity=23.7132352941176, Blast_Score=109, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 73303; Mature: 73303

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFLCEKPSQGKDIGRILGATQRGEGCLNGSGVTVTWCIGHLVEAAPPEAYDEQLKRWS
CEEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHCCCCHHHHHHHHHCC
VEQLPIIPQHWRVEVKPKTATQFKVVKALLAKATHLVIATDADREGELIAREIVELCGYR
HHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHCCC
GPIERLWLSALNDASIRAALGKLRPSAETLSMYHSALARSRADWLVGMNLSRLFTVLGRQ
CHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHC
AGYDGVLSVGRVQTPTLKLVVDRDREIARFVSVPYWAIAVSLFAGGSTFAAQWVPPDACT
CCCCHHHCCCCCCCCEEEEEECCCHHHHHHHCCCHHHHHHHHHCCCCCEEECCCCCCCCC
DDAGRCLRQPVAQQTMQQIRAAGSAHVVSVETERVREGPPLPFDLGTLQEVCSKQLGLDV
CHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHCCCH
QETLEIAQALYETHKATTYPRSDSGYLPESMFAEVPTVLDSLLKTDPSLRSIMGQLDRSQ
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
RSRAWNDGKVTAHHGIIPTLEPAKLSAMSEKELAVYRLIRSHYLAQFLPHHEFDRTVAKF
HHCCCCCCCEEEECCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
SCGGQNLAATGKQVVIPGWRQVLAEPQAEDGDGEGDTAVRAQVLPALPKLYEGLACQVAD
CCCCCCHHCCCCEEECCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEC
VDLKALKTLPPKPYTQGELVKSMKGVAKLVSDPRLKQKLKDTVGIGTEATRANIIGGLIA
CCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
RGYLVKKGRAIRASDAAFTLIDAVPAAIADPGTTAVWEQALDMIEAGQLTLDVFIGKQAA
HCHHEECCCEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEEECCHHH
WISQLIAQYGSASLSIKVPQGPACPQCGAPTRQRSGKSGPFWSCSRYPDCKGTLPVESGS
HHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
SKRGASRPRRSGRKGS
CCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRLFLCEKPSQGKDIGRILGATQRGEGCLNGSGVTVTWCIGHLVEAAPPEAYDEQLKRWS
CEEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHCCCCHHHHHHHHHCC
VEQLPIIPQHWRVEVKPKTATQFKVVKALLAKATHLVIATDADREGELIAREIVELCGYR
HHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHCCC
GPIERLWLSALNDASIRAALGKLRPSAETLSMYHSALARSRADWLVGMNLSRLFTVLGRQ
CHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHC
AGYDGVLSVGRVQTPTLKLVVDRDREIARFVSVPYWAIAVSLFAGGSTFAAQWVPPDACT
CCCCHHHCCCCCCCCEEEEEECCCHHHHHHHCCCHHHHHHHHHCCCCCEEECCCCCCCCC
DDAGRCLRQPVAQQTMQQIRAAGSAHVVSVETERVREGPPLPFDLGTLQEVCSKQLGLDV
CHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHCCCH
QETLEIAQALYETHKATTYPRSDSGYLPESMFAEVPTVLDSLLKTDPSLRSIMGQLDRSQ
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
RSRAWNDGKVTAHHGIIPTLEPAKLSAMSEKELAVYRLIRSHYLAQFLPHHEFDRTVAKF
HHCCCCCCCEEEECCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
SCGGQNLAATGKQVVIPGWRQVLAEPQAEDGDGEGDTAVRAQVLPALPKLYEGLACQVAD
CCCCCCHHCCCCEEECCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEC
VDLKALKTLPPKPYTQGELVKSMKGVAKLVSDPRLKQKLKDTVGIGTEATRANIIGGLIA
CCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
RGYLVKKGRAIRASDAAFTLIDAVPAAIADPGTTAVWEQALDMIEAGQLTLDVFIGKQAA
HCHHEECCCEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEEECCHHH
WISQLIAQYGSASLSIKVPQGPACPQCGAPTRQRSGKSGPFWSCSRYPDCKGTLPVESGS
HHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
SKRGASRPRRSGRKGS
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]