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The map label for this gene is bcsZ [H]

Identifier: 94311189

GI number: 94311189

Start: 2467411

End: 2468574

Strand: Direct

Name: bcsZ [H]

Synonym: Rmet_2251

Alternate gene names: 94311189

Gene position: 2467411-2468574 (Clockwise)

Preceding gene: 94311188

Following gene: 94311190

Centisome position: 62.81

GC content: 67.87

Gene sequence:

>1164_bases
ATGCGTCGGGCAATGGCCTGGCTGTTGCGGGCTACCGTCGTATGCACGCTGGCCGGCACGGTGTTGACCAGTGCCGCGGC
CACCTGCGCGTGGCCCGACTGGGATGCCTTCCGGCGCAGCACAATCAGCCAGGACGGCCGCGTGATCGACGACAGCACCG
ACCAGCAGGTGACGGTCTCCGAAGGCCAGTCGTATGCGCTTTTCTTCGCGCTCGTGGCCAACGACCGCGCCACCTTCGAC
AAGCTGCTGACCTGGACCGAGAACAACCTCGCGCAAGGCGACCTGACGGCCCATCTGCCCGCGTGGATCTGGGGCAAGCG
CACCGAAGGCGAGCACGCAGGCACCTGGGGTGTCATCGACGCCAATCCGGCATCGGACGCGGACCTGTGGATCGCCTACT
CCCTGCTCGAAGCCGGCCGCCTCTGGAACGAGCGCCGCTTCACGGCGCTTGGAACGCTGTTGGCGCGCCGCGCCCTGCGC
GAGGAAACGGCGGTGCTGCCCGGACTCGGCCGCACGTTGCTGCCCGGGCCCACTGGATTCCACACGTCGCCCGATGTCTG
GCGCCTGAATCCGAGTTATGTGCCATTGCAGGTCATGCGGCGGCTGGCAGCCATGCCGGGCGAGGACAGCAGCTGGAAAA
CGCTAGTCGACACCTCGGCCCGGCTGATCCTGAACACGGCGCCGCACGGCTTTTCGCCTGACTGGGTGGAGTATCACAAG
GGTCGAGGCTTCCGGCCTGATACGCAGACACAGGCCGGCGGCTCATATAACGCGATCCGGGTGTATCTCTGGGCCGGCAT
GATGGCGCCACGCGATCCGCTGCGCACTTCGGTGCTCCAGGCGTTCCGCCCGATGGCCGATTACGTCCGCAAGCAAGGGG
ATCCGCCCGAGCGGGTCGATACCCAGTCGGGCACGTTCGGACCGAACAGCGGCAACGGTGGCTTCTCGGCAGCCGTCGCG
CCCTACCTTGCCGCCCTGGGACAGACCGATGATGCGCGCGCGCAGGCGCAGCGCGCCCGCGACCTGGCCGCGCAGAAGCC
CGCCGGCTACTACAGCCAGGTGCTGGCGCTGTTCGGCCTGGGCCATCTTGACGGACATTTCCGCTTCGAAGCGGACGGCA
CTCTGGTGCCGGCCTGGAAATCCACATGCCCCGCCACGCGCTAG

Upstream 100 bases:

>100_bases
GCGCTCCTGGCGATCGCGGGCATCATTGCCGGCCTGATCGTGGCGCTGACGCTGTTCTGGGCGCTGAGCCGACTGGCGGC
ACGCCGCAACGGAGGCTGAC

Downstream 100 bases:

>100_bases
CTCCCACGCTGGCGCTGTTGCTGGCCAGTTCCGCCTTGCCGGCTTTCGCGGCCGATCCTGCCCCAGCGGCAGGTGTGTCG
CCGCCAGCCACATCCGCGCA

Product: endo-1,4-D-glucanase

Products: NA

Alternate protein names: Carboxymethylcellulase; CMCase; Cellulase; Endo-1,4-beta-glucanase [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MRRAMAWLLRATVVCTLAGTVLTSAAATCAWPDWDAFRRSTISQDGRVIDDSTDQQVTVSEGQSYALFFALVANDRATFD
KLLTWTENNLAQGDLTAHLPAWIWGKRTEGEHAGTWGVIDANPASDADLWIAYSLLEAGRLWNERRFTALGTLLARRALR
EETAVLPGLGRTLLPGPTGFHTSPDVWRLNPSYVPLQVMRRLAAMPGEDSSWKTLVDTSARLILNTAPHGFSPDWVEYHK
GRGFRPDTQTQAGGSYNAIRVYLWAGMMAPRDPLRTSVLQAFRPMADYVRKQGDPPERVDTQSGTFGPNSGNGGFSAAVA
PYLAALGQTDDARAQAQRARDLAAQKPAGYYSQVLALFGLGHLDGHFRFEADGTLVPAWKSTCPATR

Sequences:

>Translated_387_residues
MRRAMAWLLRATVVCTLAGTVLTSAAATCAWPDWDAFRRSTISQDGRVIDDSTDQQVTVSEGQSYALFFALVANDRATFD
KLLTWTENNLAQGDLTAHLPAWIWGKRTEGEHAGTWGVIDANPASDADLWIAYSLLEAGRLWNERRFTALGTLLARRALR
EETAVLPGLGRTLLPGPTGFHTSPDVWRLNPSYVPLQVMRRLAAMPGEDSSWKTLVDTSARLILNTAPHGFSPDWVEYHK
GRGFRPDTQTQAGGSYNAIRVYLWAGMMAPRDPLRTSVLQAFRPMADYVRKQGDPPERVDTQSGTFGPNSGNGGFSAAVA
PYLAALGQTDDARAQAQRARDLAAQKPAGYYSQVLALFGLGHLDGHFRFEADGTLVPAWKSTCPATR
>Mature_387_residues
MRRAMAWLLRATVVCTLAGTVLTSAAATCAWPDWDAFRRSTISQDGRVIDDSTDQQVTVSEGQSYALFFALVANDRATFD
KLLTWTENNLAQGDLTAHLPAWIWGKRTEGEHAGTWGVIDANPASDADLWIAYSLLEAGRLWNERRFTALGTLLARRALR
EETAVLPGLGRTLLPGPTGFHTSPDVWRLNPSYVPLQVMRRLAAMPGEDSSWKTLVDTSARLILNTAPHGFSPDWVEYHK
GRGFRPDTQTQAGGSYNAIRVYLWAGMMAPRDPLRTSVLQAFRPMADYVRKQGDPPERVDTQSGTFGPNSGNGGFSAAVA
PYLAALGQTDDARAQAQRARDLAAQKPAGYYSQVLALFGLGHLDGHFRFEADGTLVPAWKSTCPATR

Specific function: Hydrolyzes carboxymethylcellulose [H]

COG id: COG3405

COG function: function code G; Endoglucanase Y

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 8 (cellulase D) family [H]

Homologues:

Organism=Escherichia coli, GI1789951, Length=380, Percent_Identity=47.6315789473684, Blast_Score=336, Evalue=2e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR002037
- InterPro:   IPR019834 [H]

Pfam domain/function: PF01270 Glyco_hydro_8 [H]

EC number: =3.2.1.4 [H]

Molecular weight: Translated: 42410; Mature: 42410

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS00812 GLYCOSYL_HYDROL_F8

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRAMAWLLRATVVCTLAGTVLTSAAATCAWPDWDAFRRSTISQDGRVIDDSTDQQVTVS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEECCCCCCEEEEC
EGQSYALFFALVANDRATFDKLLTWTENNLAQGDLTAHLPAWIWGKRTEGEHAGTWGVID
CCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCHHEECCCCCCCCCCCEEEEE
ANPASDADLWIAYSLLEAGRLWNERRFTALGTLLARRALREETAVLPGLGRTLLPGPTGF
CCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
HTSPDVWRLNPSYVPLQVMRRLAAMPGEDSSWKTLVDTSARLILNTAPHGFSPDWVEYHK
CCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHCCCEEEEECCCCCCCCCHHHHHC
GRGFRPDTQTQAGGSYNAIRVYLWAGMMAPRDPLRTSVLQAFRPMADYVRKQGDPPERVD
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCC
TQSGTFGPNSGNGGFSAAVAPYLAALGQTDDARAQAQRARDLAAQKPAGYYSQVLALFGL
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
GHLDGHFRFEADGTLVPAWKSTCPATR
CCCCCEEEEECCCEEECCHHCCCCCCC
>Mature Secondary Structure
MRRAMAWLLRATVVCTLAGTVLTSAAATCAWPDWDAFRRSTISQDGRVIDDSTDQQVTVS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEECCCCCCEEEEC
EGQSYALFFALVANDRATFDKLLTWTENNLAQGDLTAHLPAWIWGKRTEGEHAGTWGVID
CCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCHHEECCCCCCCCCCCEEEEE
ANPASDADLWIAYSLLEAGRLWNERRFTALGTLLARRALREETAVLPGLGRTLLPGPTGF
CCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
HTSPDVWRLNPSYVPLQVMRRLAAMPGEDSSWKTLVDTSARLILNTAPHGFSPDWVEYHK
CCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHCCCEEEEECCCCCCCCCHHHHHC
GRGFRPDTQTQAGGSYNAIRVYLWAGMMAPRDPLRTSVLQAFRPMADYVRKQGDPPERVD
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCC
TQSGTFGPNSGNGGFSAAVAPYLAALGQTDDARAQAQRARDLAAQKPAGYYSQVLALFGL
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
GHLDGHFRFEADGTLVPAWKSTCPATR
CCCCCEEEEECCCEEECCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]