Click here to switch to the map view.

The map label for this gene is carA [H]

Identifier: 94311133

GI number: 94311133

Start: 2404866

End: 2406014

Strand: Reverse

Name: carA [H]

Synonym: Rmet_2195

Alternate gene names: 94311133

Gene position: 2406014-2404866 (Counterclockwise)

Preceding gene: 94311134

Following gene: 94311132

Centisome position: 61.25

GC content: 63.01

Gene sequence:

>1149_bases
GTGTTACCGTCTTTTCCGTCCGCCATCCTCGCGCTTGCAGACGGCACGGTCTTTCGTGGCTATTCCATCGGCGCTTCCGG
CCATACGATCGGCGAAGTGGTGTTCAACACCGCTATCACCGGTTATCAGGAAATCCTCACCGACCCGAGCTATTCCCGGC
AGATCGTCACGCTGACGTATCCGCATATCGGCAATTACGGCGTCAACCCTGAGGATGTAGAAGCCACGAAAGTCCATGCC
GCCGGCCTGATCATCAAAGATCTGCCGATTCTGGCCTCGAACTTCCGCAAGGAACATACCCTTGCCCACTACCTGAAGCA
GGAAAAGGTCGTGGCGATCGCGGGCATCGACACCCGCAAGCTGACCCGTATCCTGCGCGAGAAGGGCGCCCAGAACGGCT
GCATCCTGGCTGGCGAGGACAATGTCCAGAAGGCCATCGACCTGGCGCGCTCGTTCCCGGGCCTGGCAGGCATGGACCTG
GCCAAGGTCGTCTCCGTCAAGGAGCCGTATGAGTGGACCCAGACCGAGTGGAAGCTCGGCGAAGGCTACGGCAAGCAGGA
CAAGCCGCAGTTCCACGTGGTCGCTTTCGACTTCGGCGTCAAGTTCAACATCCTCCGCATGCTGGCCGAACGCGGCTGCA
AGGTGACCGTGCTGCCGGCGCAAGCCAGTGCGGCCGACGCCCTGGCGCTGAATCCGGACGGTATCTTCCTGTCGAACGGC
CCTGGCGACCCGGAGCCGTGCGACTACGCGATCGCCGCCACGCGTGAGTTCATTGCTCGCGGTATCCCGACCTTCGGCAT
CTGCCTGGGCCACCAGATCATGGCCCTGGCCGTCGGTGCCAAGACGCTCAAGATGAGCACGGGCCACCATGGCGCCAACC
ATCCGGTCAAGGACCATGATGATGGTCGTGTGGTGATTACGTCGCAGAACCACGGCTTCGCAGTCGATGCATCGACGCTG
CCGGCCAACGCCCGCGTCACGCACACGTCTCTGTTCGACGGCACGCTGCAAGGCTTTGCGCTGACGGACAAGCCGGCCTT
CTGCTTCCAGGGTCACCCGGAAGCGTCGCCGGGCCCGCACGACATCGCGTACCTGTTCGACCGCTTCACCAAGCTGATGC
AGGACGCCCGCGCCAAGGCCGCTGCCTGA

Upstream 100 bases:

>100_bases
TCCGCCGGGAGTCTCGCCATGCACATGGCTGAGGGCTCGCCCACGGGGCACGAGGCGTTGAGCAGGTCGGTCACGGGGTG
AATCCAGCAGGAGCCTACCC

Downstream 100 bases:

>100_bases
GATCGAGAAACGAGAGCCTGAACGGGAAACGCCCATGAACGCCTTCATGCATGCCAACCTCGGCATCACTGATTTCTGGA
CCTTTGTGCTCGGTACGATC

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MLPSFPSAILALADGTVFRGYSIGASGHTIGEVVFNTAITGYQEILTDPSYSRQIVTLTYPHIGNYGVNPEDVEATKVHA
AGLIIKDLPILASNFRKEHTLAHYLKQEKVVAIAGIDTRKLTRILREKGAQNGCILAGEDNVQKAIDLARSFPGLAGMDL
AKVVSVKEPYEWTQTEWKLGEGYGKQDKPQFHVVAFDFGVKFNILRMLAERGCKVTVLPAQASAADALALNPDGIFLSNG
PGDPEPCDYAIAATREFIARGIPTFGICLGHQIMALAVGAKTLKMSTGHHGANHPVKDHDDGRVVITSQNHGFAVDASTL
PANARVTHTSLFDGTLQGFALTDKPAFCFQGHPEASPGPHDIAYLFDRFTKLMQDARAKAAA

Sequences:

>Translated_382_residues
MLPSFPSAILALADGTVFRGYSIGASGHTIGEVVFNTAITGYQEILTDPSYSRQIVTLTYPHIGNYGVNPEDVEATKVHA
AGLIIKDLPILASNFRKEHTLAHYLKQEKVVAIAGIDTRKLTRILREKGAQNGCILAGEDNVQKAIDLARSFPGLAGMDL
AKVVSVKEPYEWTQTEWKLGEGYGKQDKPQFHVVAFDFGVKFNILRMLAERGCKVTVLPAQASAADALALNPDGIFLSNG
PGDPEPCDYAIAATREFIARGIPTFGICLGHQIMALAVGAKTLKMSTGHHGANHPVKDHDDGRVVITSQNHGFAVDASTL
PANARVTHTSLFDGTLQGFALTDKPAFCFQGHPEASPGPHDIAYLFDRFTKLMQDARAKAAA
>Mature_382_residues
MLPSFPSAILALADGTVFRGYSIGASGHTIGEVVFNTAITGYQEILTDPSYSRQIVTLTYPHIGNYGVNPEDVEATKVHA
AGLIIKDLPILASNFRKEHTLAHYLKQEKVVAIAGIDTRKLTRILREKGAQNGCILAGEDNVQKAIDLARSFPGLAGMDL
AKVVSVKEPYEWTQTEWKLGEGYGKQDKPQFHVVAFDFGVKFNILRMLAERGCKVTVLPAQASAADALALNPDGIFLSNG
PGDPEPCDYAIAATREFIARGIPTFGICLGHQIMALAVGAKTLKMSTGHHGANHPVKDHDDGRVVITSQNHGFAVDASTL
PANARVTHTSLFDGTLQGFALTDKPAFCFQGHPEASPGPHDIAYLFDRFTKLMQDARAKAAA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=384, Percent_Identity=38.8020833333333, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI169790915, Length=385, Percent_Identity=36.3636363636364, Blast_Score=215, Evalue=4e-56,
Organism=Homo sapiens, GI21361331, Length=385, Percent_Identity=36.3636363636364, Blast_Score=215, Evalue=5e-56,
Organism=Escherichia coli, GI1786215, Length=378, Percent_Identity=66.1375661375661, Blast_Score=523, Evalue=1e-150,
Organism=Escherichia coli, GI1789760, Length=136, Percent_Identity=30.8823529411765, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=384, Percent_Identity=38.8020833333333, Blast_Score=253, Evalue=9e-68,
Organism=Saccharomyces cerevisiae, GI6322331, Length=389, Percent_Identity=35.9897172236504, Blast_Score=239, Evalue=5e-64,
Organism=Saccharomyces cerevisiae, GI6324878, Length=390, Percent_Identity=36.6666666666667, Blast_Score=235, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6322638, Length=176, Percent_Identity=30.6818181818182, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45555749, Length=384, Percent_Identity=41.40625, Blast_Score=247, Evalue=1e-65,
Organism=Drosophila melanogaster, GI24642586, Length=384, Percent_Identity=41.40625, Blast_Score=246, Evalue=1e-65,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 41103; Mature: 41103

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPSFPSAILALADGTVFRGYSIGASGHTIGEVVFNTAITGYQEILTDPSYSRQIVTLTY
CCCCCCCHHHHHCCCCEEECEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
PHIGNYGVNPEDVEATKVHAAGLIIKDLPILASNFRKEHTLAHYLKQEKVVAIAGIDTRK
CCCCCCCCCCCCCHHHEEEECCEEECCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHH
LTRILREKGAQNGCILAGEDNVQKAIDLARSFPGLAGMDLAKVVSVKEPYEWTQTEWKLG
HHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCEEEC
EGYGKQDKPQFHVVAFDFGVKFNILRMLAERGCKVTVLPAQASAADALALNPDGIFLSNG
CCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCEEEECC
PGDPEPCDYAIAATREFIARGIPTFGICLGHQIMALAVGAKTLKMSTGHHGANHPVKDHD
CCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCC
DGRVVITSQNHGFAVDASTLPANARVTHTSLFDGTLQGFALTDKPAFCFQGHPEASPGPH
CCEEEEEECCCCEEEECCCCCCCCEEEEEEHHCCCCCCEEECCCCCEEECCCCCCCCCHH
DIAYLFDRFTKLMQDARAKAAA
HHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLPSFPSAILALADGTVFRGYSIGASGHTIGEVVFNTAITGYQEILTDPSYSRQIVTLTY
CCCCCCCHHHHHCCCCEEECEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
PHIGNYGVNPEDVEATKVHAAGLIIKDLPILASNFRKEHTLAHYLKQEKVVAIAGIDTRK
CCCCCCCCCCCCCHHHEEEECCEEECCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHH
LTRILREKGAQNGCILAGEDNVQKAIDLARSFPGLAGMDLAKVVSVKEPYEWTQTEWKLG
HHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCEEEC
EGYGKQDKPQFHVVAFDFGVKFNILRMLAERGCKVTVLPAQASAADALALNPDGIFLSNG
CCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCEEEECC
PGDPEPCDYAIAATREFIARGIPTFGICLGHQIMALAVGAKTLKMSTGHHGANHPVKDHD
CCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCC
DGRVVITSQNHGFAVDASTLPANARVTHTSLFDGTLQGFALTDKPAFCFQGHPEASPGPH
CCEEEEEECCCCEEEECCCCCCCCEEEEEEHHCCCCCCEEECCCCCEEECCCCCCCCCHH
DIAYLFDRFTKLMQDARAKAAA
HHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]