Click here to switch to the map view.

The map label for this gene is rrmJ [H]

Identifier: 94311127

GI number: 94311127

Start: 2398223

End: 2398888

Strand: Reverse

Name: rrmJ [H]

Synonym: Rmet_2189

Alternate gene names: 94311127

Gene position: 2398888-2398223 (Counterclockwise)

Preceding gene: 94311129

Following gene: 94311126

Centisome position: 61.07

GC content: 61.56

Gene sequence:

>666_bases
ATGGCCAAGAACAAATTCAATCAGTCGTGGCTGCACGACCACATCAACGATCCGTACGTAAAGATGGCCCAGCGCGAGGG
CTATCGCGCCCGCGCGGCCTACAAGCTCAAAGAGATCGACGATCAGGACAAGCTGATCCAGCCGGGCCAGGTCATCGTGG
ATCTGGGCGCCGCGCCGGGCAGCTGGAGCCAGTACGCCCGGAACAAGCTGGCCGCGTCGCCCCGCGCGAAAGACGGCCGG
ATCGACGGCGCCGTGGTGGCGATCGACATCCTGCCGATGGAGCCGGTGGCCGACGTAACCTTTATCCAGGGCGACTTCCG
GGAAGAAGAAGTGTTCCGTCAGCTCGAACAGGTGGTGCTGGATGCGTCTGGCGGCTCCCGAATCGACCTTGTTATCTCCG
ATATGGCCCCCAACTTGTCTGGCGTGGCCTCGGCCGATGCCGCGCGGATCGAGTATCTGTGCGATCTGGCGCTGGACTTT
GCCCAGGCCCACCTGAAGCCGGAAGGTTCCTTGCTGGTCAAGTGCTTTCACGGCTCGGGCTACAGCCAGATCGTGGAAAA
GTTCAAGCGGCAGTTCAAGGTCGTCGCGAGCCGCAAGCCCAAGGCGTCGCGTGACAAGTCTTCGGAAACATTTATTCTTG
GGCGCTACCTGAAGTCGGTGGACTGA

Upstream 100 bases:

>100_bases
GTAAAGATCTCCAAATCGGGAGAAAAAGCTCCGCACGGTCGTTCGACTTGCGGTGCCAATCCCGCCAAACCGGTAAGTAG
CAAGTAGGCAAGTCTCATCA

Downstream 100 bases:

>100_bases
CGCGGGCCCAAAACATGCCGCAGGCGCTCCCAGTGCTCCCAAACGGGGGCAGGGTACGCCCGGGCGGCGCGCGACTTGCC
CTGAATGGGCCGTATCAGGC

Product: 23S rRNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MAKNKFNQSWLHDHINDPYVKMAQREGYRARAAYKLKEIDDQDKLIQPGQVIVDLGAAPGSWSQYARNKLAASPRAKDGR
IDGAVVAIDILPMEPVADVTFIQGDFREEEVFRQLEQVVLDASGGSRIDLVISDMAPNLSGVASADAARIEYLCDLALDF
AQAHLKPEGSLLVKCFHGSGYSQIVEKFKRQFKVVASRKPKASRDKSSETFILGRYLKSVD

Sequences:

>Translated_221_residues
MAKNKFNQSWLHDHINDPYVKMAQREGYRARAAYKLKEIDDQDKLIQPGQVIVDLGAAPGSWSQYARNKLAASPRAKDGR
IDGAVVAIDILPMEPVADVTFIQGDFREEEVFRQLEQVVLDASGGSRIDLVISDMAPNLSGVASADAARIEYLCDLALDF
AQAHLKPEGSLLVKCFHGSGYSQIVEKFKRQFKVVASRKPKASRDKSSETFILGRYLKSVD
>Mature_220_residues
AKNKFNQSWLHDHINDPYVKMAQREGYRARAAYKLKEIDDQDKLIQPGQVIVDLGAAPGSWSQYARNKLAASPRAKDGRI
DGAVVAIDILPMEPVADVTFIQGDFREEEVFRQLEQVVLDASGGSRIDLVISDMAPNLSGVASADAARIEYLCDLALDFA
QAHLKPEGSLLVKCFHGSGYSQIVEKFKRQFKVVASRKPKASRDKSSETFILGRYLKSVD

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=205, Percent_Identity=35.609756097561, Blast_Score=134, Evalue=9e-32,
Organism=Homo sapiens, GI29029591, Length=195, Percent_Identity=34.8717948717949, Blast_Score=107, Evalue=8e-24,
Organism=Homo sapiens, GI29029589, Length=195, Percent_Identity=34.8717948717949, Blast_Score=107, Evalue=8e-24,
Organism=Homo sapiens, GI7110661, Length=195, Percent_Identity=34.8717948717949, Blast_Score=107, Evalue=9e-24,
Organism=Homo sapiens, GI194097365, Length=197, Percent_Identity=29.9492385786802, Blast_Score=100, Evalue=8e-22,
Organism=Escherichia coli, GI1789569, Length=204, Percent_Identity=43.1372549019608, Blast_Score=182, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17554650, Length=197, Percent_Identity=34.5177664974619, Blast_Score=110, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17553474, Length=230, Percent_Identity=30.4347826086957, Blast_Score=99, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71987550, Length=197, Percent_Identity=31.4720812182741, Blast_Score=99, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71987561, Length=197, Percent_Identity=31.4720812182741, Blast_Score=99, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17553860, Length=197, Percent_Identity=31.4720812182741, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6319535, Length=199, Percent_Identity=32.6633165829146, Blast_Score=105, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6321302, Length=283, Percent_Identity=27.9151943462898, Blast_Score=95, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6319796, Length=204, Percent_Identity=29.9019607843137, Blast_Score=95, Evalue=8e-21,
Organism=Drosophila melanogaster, GI21356387, Length=218, Percent_Identity=35.3211009174312, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24647580, Length=197, Percent_Identity=31.9796954314721, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24648639, Length=199, Percent_Identity=31.6582914572864, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI18859957, Length=197, Percent_Identity=29.4416243654822, Blast_Score=91, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 24543; Mature: 24412

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNKFNQSWLHDHINDPYVKMAQREGYRARAAYKLKEIDDQDKLIQPGQVIVDLGAAPG
CCCCCCCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCEEEEECCCCCC
SWSQYARNKLAASPRAKDGRIDGAVVAIDILPMEPVADVTFIQGDFREEEVFRQLEQVVL
CHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHH
DASGGSRIDLVISDMAPNLSGVASADAARIEYLCDLALDFAQAHLKPEGSLLVKCFHGSG
CCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
YSQIVEKFKRQFKVVASRKPKASRDKSSETFILGRYLKSVD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKNKFNQSWLHDHINDPYVKMAQREGYRARAAYKLKEIDDQDKLIQPGQVIVDLGAAPG
CCCCCCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCEEEEECCCCCC
SWSQYARNKLAASPRAKDGRIDGAVVAIDILPMEPVADVTFIQGDFREEEVFRQLEQVVL
CHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHH
DASGGSRIDLVISDMAPNLSGVASADAARIEYLCDLALDFAQAHLKPEGSLLVKCFHGSG
CCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
YSQIVEKFKRQFKVVASRKPKASRDKSSETFILGRYLKSVD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA