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The map label for this gene is gltX [H]

Identifier: 94311078

GI number: 94311078

Start: 2330543

End: 2331940

Strand: Reverse

Name: gltX [H]

Synonym: Rmet_2140

Alternate gene names: 94311078

Gene position: 2331940-2330543 (Counterclockwise)

Preceding gene: 94311080

Following gene: 94311071

Centisome position: 59.37

GC content: 62.95

Gene sequence:

>1398_bases
ATGACACAACGCGTCCGCACCCGCTTCGCACCGAGTCCGACCGGCTTCATTCACCTCGGCAATATTCGCTCCGCGTTTTA
TCCGTGGGCGTTTGCCCGCCGCATGAAGGGCGACTTCATCCTGCGTATCGAGGATACCGACGTCGAGCGTTCGACCGATG
TCGCCGTCGACGTCATCCTGGAAAGCATGGCCTGGCTCGATCTGGACATCGACGAAGGCCCCTTCTATCAGATGCAGCGG
ATGGACCGGTATCGCGAGGTGGTACAGCAGATGCTCGACAACGAGCTGGCTTACCACTGCTATATGAGTACCGAGGAGCT
TGACGCCTTGCGCGAAGCGCAGCGTGCTGCTGGCGAAAAGCCGCGGTACAACGGTTTCTGGCGTCCGGAGCCGGGCAAGG
TGCTGCCCGAGCCGCCGGCTGGCGTGCAGCCCGTGGTGCGCTTCAAAAACCCGATCGGCGGTAGCGTGGTGTGGGACGAC
GCCGTGAAGGGCCGCATCGAGATCTCGAATGATGAACTCGACGATCTCGTCATTGCCCGCCCCGATGGCACGCCGACCTA
CAACTTCTGCGTGGTGGTGGATGACCTCGACATGAAGATCACCCACGTGATCCGGGGCGATGACCACGTCAATAACACGC
CGCGGCAGATCAACATCATCCGCGCCCTGGGTGGCGAGGTGCCGGTCTACGCCCACCTGCCCACCGTGCTCAACGAGCAG
GGTGAGAAGATGAGTAAGCGTCATGGCGCGTTGCCCGTCACGGGATATCGGGATGAGGGCTATCTGCCGGAGGCGGTCCT
GAACTATCTGGCGCGACTGGGCTGGGCGCATGGCGATGCCGAGATCTTCTCGCGCGAACAGTTCGTCGAATGGTTCGATC
TGGAGCACTTGGGCAAGTCCCCGGCGCAATACAACCCGGAAAAGCTGGCGTGGCTCAACAACCATTACATCAAGGTTGGT
GACAACACGCGCCTGGCAGACCTGACGCGTCCGTTCATCGAGGCGCTCGGCGGCAAGGTCGAAGGCGCGAACCTGGTGGA
CGTGATCGCGCTGGTCAAGGATCGCGCGAACACGCTGAAGGAAGTGGCGCAGACCGCGATGCTGTTTTATCGCGGCGAGC
CGCAGGCCGATGCGGCGCTGAAGGCCGAACACCTGACGGACGAGATTCGCCCCGCACTGCAGGCGCTGGCCACGCAACTC
GCGGCGCTGCCGGAGTGGAAGCGCGAAGCGATCAGTGCCGCGTTCAAGGCAGTGCTGGCGGAGTTCGGTCTGAAGATGCC
GAAGCTGGCGATGCCGGTGCGCCTGCTGGTGGCGGGACAGTTGCAGACGCCGAGCATCGACGCGGTGCTTGAACTGTTCG
GTCGCGACACGGTGCTGCGCCGTTTGGCTGCAGCGTAA

Upstream 100 bases:

>100_bases
TTCCAGGGCGGAGATCGTGTCGGGCAGCGCGTATAATGCAGCATTCCCTGACATCCGGCCTGCGCGCATCCGTGCGCGGG
CCTTTTTGTTTCCCCGAACC

Downstream 100 bases:

>100_bases
AAAACTGCTGAAAAAGTCTCGGCTGGGGCTTGCGCTCTGGTCGAGACTTCAGCATAATCTCGTTCTCCCTGCTGCACGAC
GCGAGTCAAGCAGAGCGGTT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MTQRVRTRFAPSPTGFIHLGNIRSAFYPWAFARRMKGDFILRIEDTDVERSTDVAVDVILESMAWLDLDIDEGPFYQMQR
MDRYREVVQQMLDNELAYHCYMSTEELDALREAQRAAGEKPRYNGFWRPEPGKVLPEPPAGVQPVVRFKNPIGGSVVWDD
AVKGRIEISNDELDDLVIARPDGTPTYNFCVVVDDLDMKITHVIRGDDHVNNTPRQINIIRALGGEVPVYAHLPTVLNEQ
GEKMSKRHGALPVTGYRDEGYLPEAVLNYLARLGWAHGDAEIFSREQFVEWFDLEHLGKSPAQYNPEKLAWLNNHYIKVG
DNTRLADLTRPFIEALGGKVEGANLVDVIALVKDRANTLKEVAQTAMLFYRGEPQADAALKAEHLTDEIRPALQALATQL
AALPEWKREAISAAFKAVLAEFGLKMPKLAMPVRLLVAGQLQTPSIDAVLELFGRDTVLRRLAAA

Sequences:

>Translated_465_residues
MTQRVRTRFAPSPTGFIHLGNIRSAFYPWAFARRMKGDFILRIEDTDVERSTDVAVDVILESMAWLDLDIDEGPFYQMQR
MDRYREVVQQMLDNELAYHCYMSTEELDALREAQRAAGEKPRYNGFWRPEPGKVLPEPPAGVQPVVRFKNPIGGSVVWDD
AVKGRIEISNDELDDLVIARPDGTPTYNFCVVVDDLDMKITHVIRGDDHVNNTPRQINIIRALGGEVPVYAHLPTVLNEQ
GEKMSKRHGALPVTGYRDEGYLPEAVLNYLARLGWAHGDAEIFSREQFVEWFDLEHLGKSPAQYNPEKLAWLNNHYIKVG
DNTRLADLTRPFIEALGGKVEGANLVDVIALVKDRANTLKEVAQTAMLFYRGEPQADAALKAEHLTDEIRPALQALATQL
AALPEWKREAISAAFKAVLAEFGLKMPKLAMPVRLLVAGQLQTPSIDAVLELFGRDTVLRRLAAA
>Mature_464_residues
TQRVRTRFAPSPTGFIHLGNIRSAFYPWAFARRMKGDFILRIEDTDVERSTDVAVDVILESMAWLDLDIDEGPFYQMQRM
DRYREVVQQMLDNELAYHCYMSTEELDALREAQRAAGEKPRYNGFWRPEPGKVLPEPPAGVQPVVRFKNPIGGSVVWDDA
VKGRIEISNDELDDLVIARPDGTPTYNFCVVVDDLDMKITHVIRGDDHVNNTPRQINIIRALGGEVPVYAHLPTVLNEQG
EKMSKRHGALPVTGYRDEGYLPEAVLNYLARLGWAHGDAEIFSREQFVEWFDLEHLGKSPAQYNPEKLAWLNNHYIKVGD
NTRLADLTRPFIEALGGKVEGANLVDVIALVKDRANTLKEVAQTAMLFYRGEPQADAALKAEHLTDEIRPALQALATQLA
ALPEWKREAISAAFKAVLAEFGLKMPKLAMPVRLLVAGQLQTPSIDAVLELFGRDTVLRRLAAA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=331, Percent_Identity=33.5347432024169, Blast_Score=183, Evalue=3e-46,
Organism=Escherichia coli, GI1788743, Length=463, Percent_Identity=49.244060475162, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI226510925, Length=285, Percent_Identity=32.280701754386, Blast_Score=128, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI71993394, Length=481, Percent_Identity=30.1455301455301, Blast_Score=199, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6324540, Length=330, Percent_Identity=35.4545454545455, Blast_Score=203, Evalue=5e-53,
Organism=Drosophila melanogaster, GI24665321, Length=493, Percent_Identity=29.2089249492901, Blast_Score=221, Evalue=6e-58,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 52295; Mature: 52164

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQRVRTRFAPSPTGFIHLGNIRSAFYPWAFARRMKGDFILRIEDTDVERSTDVAVDVIL
CCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHH
ESMAWLDLDIDEGPFYQMQRMDRYREVVQQMLDNELAYHCYMSTEELDALREAQRAAGEK
HHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCC
PRYNGFWRPEPGKVLPEPPAGVQPVVRFKNPIGGSVVWDDAVKGRIEISNDELDDLVIAR
CCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCEEECCCCCCEEEECCCCCCCEEEEC
PDGTPTYNFCVVVDDLDMKITHVIRGDDHVNNTPRQINIIRALGGEVPVYAHLPTVLNEQ
CCCCCCEEEEEEEECCCCEEEEEECCCCCCCCCCHHEEEEECCCCCCCEEEECCHHHHHH
GEKMSKRHGALPVTGYRDEGYLPEAVLNYLARLGWAHGDAEIFSREQFVEWFDLEHLGKS
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
PAQYNPEKLAWLNNHYIKVGDNTRLADLTRPFIEALGGKVEGANLVDVIALVKDRANTLK
CCCCCCHHEEEECCCEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
EVAQTAMLFYRGEPQADAALKAEHLTDEIRPALQALATQLAALPEWKREAISAAFKAVLA
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
EFGLKMPKLAMPVRLLVAGQLQTPSIDAVLELFGRDTVLRRLAAA
HHCCCCCCHHHCHHHEEECCCCCCCHHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure 
TQRVRTRFAPSPTGFIHLGNIRSAFYPWAFARRMKGDFILRIEDTDVERSTDVAVDVIL
CCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHH
ESMAWLDLDIDEGPFYQMQRMDRYREVVQQMLDNELAYHCYMSTEELDALREAQRAAGEK
HHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCC
PRYNGFWRPEPGKVLPEPPAGVQPVVRFKNPIGGSVVWDDAVKGRIEISNDELDDLVIAR
CCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCEEECCCCCCEEEECCCCCCCEEEEC
PDGTPTYNFCVVVDDLDMKITHVIRGDDHVNNTPRQINIIRALGGEVPVYAHLPTVLNEQ
CCCCCCEEEEEEEECCCCEEEEEECCCCCCCCCCHHEEEEECCCCCCCEEEECCHHHHHH
GEKMSKRHGALPVTGYRDEGYLPEAVLNYLARLGWAHGDAEIFSREQFVEWFDLEHLGKS
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
PAQYNPEKLAWLNNHYIKVGDNTRLADLTRPFIEALGGKVEGANLVDVIALVKDRANTLK
CCCCCCHHEEEECCCEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
EVAQTAMLFYRGEPQADAALKAEHLTDEIRPALQALATQLAALPEWKREAISAAFKAVLA
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
EFGLKMPKLAMPVRLLVAGQLQTPSIDAVLELFGRDTVLRRLAAA
HHCCCCCCHHHCHHHEEECCCCCCCHHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA