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The map label for this gene is nirJ

Identifier: 94311020

GI number: 94311020

Start: 2260352

End: 2261317

Strand: Direct

Name: nirJ

Synonym: Rmet_2082

Alternate gene names: NA

Gene position: 2260352-2261317 (Clockwise)

Preceding gene: 335055580

Following gene: 94311021

Centisome position: 57.54

GC content: 65.01

Gene sequence:

>966_bases
ATGCTCCGCGTGTCCCGATTCATGGAAGCGCTGTGCGACGGCGCGCCAACCTCCGCCCTGCCCCGCGATCGCCGCAATGA
CGAAAGCCCCGTTGTCATCTGGCATCCGTGCGCGCACGACCCGTCCACGGCCGGCACCGGCACCGGCACCGGCACCGAAC
TCGATACCACCGAGTCCCTGGCGTTGCTCGAGCAACTCCATCTCGCCCGGGTTCGCTCGCTGATCCTCTCTGGCAGCGAT
CCGATGGCACGCTCCGACTTCTTCAAGATCATCGCCCGGGCCCGCGAGCTGGACCTCAATGTGCTGCTGTCCACGGACGG
TAGCCGCCTGACAGGCATGAACGCCGTCAAGCTGGCTGCCTATGGTCTCGACTACGTCGGTATCCAGATCGACGGGCTGC
AGTACACGCACAACCATGTGCAACGCTCCCGCCACGCCTACCAACGCGCCTTGCGCGGCCTGCGTGCTGCCCGCAACGCC
CACCTGAGAGTCGGGATGCGCATGACACTGAGCCCGATGAACGCTGCGGAGTTGCCCGCGGTGGTGGCCCTGGCGGACAG
CGAGCGCATCGACAGGTTTCATCTCTCTCAGCCCTGCCCTGCGGACTGCGCGCACGACTCCGCCCAGAACACATCACTTC
GTGACATGACGCGTGCTTCGCTCGAGTGGCTGTTCGATTATGTGTGGATGCGAGCGCAGAAAGGCAATGCAGGCGACTTC
GTCACCAGCCAGCACGCGGCCAGCAGCGCCTATCTGCTGCATTGGGTCTCAACACGCGCGCCACACCATCTGGCGGCCAT
TCGCGAGCGTATCGCGTGCGCCAGCAAGGCCAAGGGCGCTGGCATCCTTCTGATCGATGCGCTCGGCCGCGTCCATCGGG
GCTCGGCTCGGCTCGATGAAACTTGCGGCAATGTACGCGAACGACCGTTCAGCGACATCGCCGAGCAACGCTTGATCCAC
GCGTAG

Upstream 100 bases:

>100_bases
CGTTGCTGGGTGATGCCTGCCGGGCCCACGACGTACTCTGGTCGACCCGGATCCTCAAGAAAACCGGTCTTCGGCTGCCA
GCCACAGGAAACGTCTGATC

Downstream 100 bases:

>100_bases
TTCGCCAGAGCTACGCCGATAGTACGCGCCAGCATCTACTTTGATATTCGACAAGAGTGGGACACGGAGCCTGTGCCACG
CTTGCCCGTCACTACTACGC

Product: Heme d1 biosynthesis protein NirJ

Products: NA

Alternate protein names: Radical SAM Domain-Containing Protein; Radical SAM Family Protein; Fe-S Oxidoreductase; Fe-S Oxidoreductases; Nitrite Reductase Heme Biosynthesis J Protein; Elongator /MiaB/NifB; Heme D1 Biosynthesis Protein NirJ; Metallo Cofactor Biosynthesis Protein; Heme Biosynthesis Protein; Molybdopterin-Based TUNGsten Cofactor Biosynthesis; Homolog Co PQQ Synthesis Protein E; Nitrite Reductase Protein N; Radical Sam Domain Protein; Nitrite Reductase Heme D1 Biosynthesis Protein; Co PQQ Synthesis Protein E-Like Protein; Heme D1 Biosynthesis Protein; Radical SAM Superfamily Protein; MoaA/NirJ Family Fe-S Oxidoreductase; Elongator /Miab/Nifb; Heme D1 Biosynthesis Protein NIRJ

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MLRVSRFMEALCDGAPTSALPRDRRNDESPVVIWHPCAHDPSTAGTGTGTGTELDTTESLALLEQLHLARVRSLILSGSD
PMARSDFFKIIARARELDLNVLLSTDGSRLTGMNAVKLAAYGLDYVGIQIDGLQYTHNHVQRSRHAYQRALRGLRAARNA
HLRVGMRMTLSPMNAAELPAVVALADSERIDRFHLSQPCPADCAHDSAQNTSLRDMTRASLEWLFDYVWMRAQKGNAGDF
VTSQHAASSAYLLHWVSTRAPHHLAAIRERIACASKAKGAGILLIDALGRVHRGSARLDETCGNVRERPFSDIAEQRLIH
A

Sequences:

>Translated_321_residues
MLRVSRFMEALCDGAPTSALPRDRRNDESPVVIWHPCAHDPSTAGTGTGTGTELDTTESLALLEQLHLARVRSLILSGSD
PMARSDFFKIIARARELDLNVLLSTDGSRLTGMNAVKLAAYGLDYVGIQIDGLQYTHNHVQRSRHAYQRALRGLRAARNA
HLRVGMRMTLSPMNAAELPAVVALADSERIDRFHLSQPCPADCAHDSAQNTSLRDMTRASLEWLFDYVWMRAQKGNAGDF
VTSQHAASSAYLLHWVSTRAPHHLAAIRERIACASKAKGAGILLIDALGRVHRGSARLDETCGNVRERPFSDIAEQRLIH
A
>Mature_321_residues
MLRVSRFMEALCDGAPTSALPRDRRNDESPVVIWHPCAHDPSTAGTGTGTGTELDTTESLALLEQLHLARVRSLILSGSD
PMARSDFFKIIARARELDLNVLLSTDGSRLTGMNAVKLAAYGLDYVGIQIDGLQYTHNHVQRSRHAYQRALRGLRAARNA
HLRVGMRMTLSPMNAAELPAVVALADSERIDRFHLSQPCPADCAHDSAQNTSLRDMTRASLEWLFDYVWMRAQKGNAGDF
VTSQHAASSAYLLHWVSTRAPHHLAAIRERIACASKAKGAGILLIDALGRVHRGSARLDETCGNVRERPFSDIAEQRLIH
A

Specific function: Unknown

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 35331; Mature: 35331

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRVSRFMEALCDGAPTSALPRDRRNDESPVVIWHPCAHDPSTAGTGTGTGTELDTTESL
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHH
ALLEQLHLARVRSLILSGSDPMARSDFFKIIARARELDLNVLLSTDGSRLTGMNAVKLAA
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHH
YGLDYVGIQIDGLQYTHNHVQRSRHAYQRALRGLRAARNAHLRVGMRMTLSPMNAAELPA
HCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECEEEEECCCCHHHCCE
VVALADSERIDRFHLSQPCPADCAHDSAQNTSLRDMTRASLEWLFDYVWMRAQKGNAGDF
EEEECCCHHHHHHCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
VTSQHAASSAYLLHWVSTRAPHHLAAIRERIACASKAKGAGILLIDALGRVHRGSARLDE
CCCHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHCCCCHHHH
TCGNVRERPFSDIAEQRLIHA
HHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure
MLRVSRFMEALCDGAPTSALPRDRRNDESPVVIWHPCAHDPSTAGTGTGTGTELDTTESL
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHH
ALLEQLHLARVRSLILSGSDPMARSDFFKIIARARELDLNVLLSTDGSRLTGMNAVKLAA
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHH
YGLDYVGIQIDGLQYTHNHVQRSRHAYQRALRGLRAARNAHLRVGMRMTLSPMNAAELPA
HCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECEEEEECCCCHHHCCE
VVALADSERIDRFHLSQPCPADCAHDSAQNTSLRDMTRASLEWLFDYVWMRAQKGNAGDF
EEEECCCHHHHHHCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
VTSQHAASSAYLLHWVSTRAPHHLAAIRERIACASKAKGAGILLIDALGRVHRGSARLDE
CCCHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHCCCCHHHH
TCGNVRERPFSDIAEQRLIHA
HHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA