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The map label for this gene is dus [H]

Identifier: 94310927

GI number: 94310927

Start: 2162059

End: 2163114

Strand: Reverse

Name: dus [H]

Synonym: Rmet_1989

Alternate gene names: 94310927

Gene position: 2163114-2162059 (Counterclockwise)

Preceding gene: 335055573

Following gene: 94310926

Centisome position: 55.07

GC content: 58.33

Gene sequence:

>1056_bases
ATGGAACAGACCAGTAGCACCCCACAAACCGAGTCCAGAGGCGGAAGCCAAGGAAATATCAGGCCCCGTAGAATTTCTGT
GGCGCCAATGATGGACTGGACTGACCGCCATTGTCGGACGTTTCACCGCGCCCTGAGTCAACACACCTGGCTCTATACGG
AGATGGTGACGACTGGTGCGCTGCTGCACGGCGATGTCGCGCGGCACCTCGATTTCAGTGCGGCGGAGCAACCTGTGGCG
CTGCAGCTTGGCGGCAGCGAGCCGGTGGACCTTGCCCAAGCGGCAAAACTGGGCGAGCAGTGGGGCTACAAAGAGATCAA
TCTGAACTGCGGTTGTCCGTCCGAGCGCGTGCAACGCGGTGCGTTTGGTGCGTGCCTGATGGCCGAACCACAGCTTGTGG
CCGATTGTGTGAGGGCCATGCGCGATGTCGTGTCGGTGCCGGTGACGGTCAAGCACCGCATTGGCATTGATACGATCGAG
CACTACGGATTTGTGCGTGATTTTGTCGGCACCATTGCGGAGGCCGGCTGTGACATATTCATCGTCCACGCGCGTAATGC
GATTCTGAAGGGGCTGAGCCCAAAGGAGAATCGCGAAATTCCACCGCTGCGATATGAGGTCGCCTATGAACTAAAGCGGG
AATTTCCACATCTGGAAATCCTGATTAATGGTGGTGTTGTCTCGTACGGTGAAATAGACGAACACCTGAGGCACGTCGAC
GGCGTAATGATCGGGCGTCAGGCTTATCACGATCCCTATTTCCTCTCGGAAATGGATGCACGTTTCTATGGTGGGACTGC
GCCTATCGTGACGCGCGAAATGGCCGAGCTGACGATGCAGGCATATATTGGCGATCTCGTGGAGAAGGGTGGCTATATGG
GGGCTGCAACGCGCCATATGCTTGGCCTCCATCGCGGAATCTATGGTGGCCGTGGTTGGCGTCGTGTGCTTTCCGATGCC
CGTCGAATGAACGCAGCGCGCTCCCGAGCAGATGTGGATGCCCTGTTCGAGGAGGCGCGCTCACACCTTCGTCCGGGGCT
CCAGGAAGCGGCGTAA

Upstream 100 bases:

>100_bases
CGTGATCGTCCCCATGGTCCCGGTGCTACGCGAGTAGTTGTGTGCTACATCGTGGCACTTTGTTCGGAAAAAGGGACGAA
ACAGGCAGAATCAGTAGGCA

Downstream 100 bases:

>100_bases
TTTGATGTCAGTCAGATTTCTGCATGTCGAGGAAAGGTTCTAAAAACGAACGCTCGTTCCATGCGACCGGGCGCAAATGT
ATGCTGCTGAGGCGTTTGCG

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MEQTSSTPQTESRGGSQGNIRPRRISVAPMMDWTDRHCRTFHRALSQHTWLYTEMVTTGALLHGDVARHLDFSAAEQPVA
LQLGGSEPVDLAQAAKLGEQWGYKEINLNCGCPSERVQRGAFGACLMAEPQLVADCVRAMRDVVSVPVTVKHRIGIDTIE
HYGFVRDFVGTIAEAGCDIFIVHARNAILKGLSPKENREIPPLRYEVAYELKREFPHLEILINGGVVSYGEIDEHLRHVD
GVMIGRQAYHDPYFLSEMDARFYGGTAPIVTREMAELTMQAYIGDLVEKGGYMGAATRHMLGLHRGIYGGRGWRRVLSDA
RRMNAARSRADVDALFEEARSHLRPGLQEAA

Sequences:

>Translated_351_residues
MEQTSSTPQTESRGGSQGNIRPRRISVAPMMDWTDRHCRTFHRALSQHTWLYTEMVTTGALLHGDVARHLDFSAAEQPVA
LQLGGSEPVDLAQAAKLGEQWGYKEINLNCGCPSERVQRGAFGACLMAEPQLVADCVRAMRDVVSVPVTVKHRIGIDTIE
HYGFVRDFVGTIAEAGCDIFIVHARNAILKGLSPKENREIPPLRYEVAYELKREFPHLEILINGGVVSYGEIDEHLRHVD
GVMIGRQAYHDPYFLSEMDARFYGGTAPIVTREMAELTMQAYIGDLVEKGGYMGAATRHMLGLHRGIYGGRGWRRVLSDA
RRMNAARSRADVDALFEEARSHLRPGLQEAA
>Mature_351_residues
MEQTSSTPQTESRGGSQGNIRPRRISVAPMMDWTDRHCRTFHRALSQHTWLYTEMVTTGALLHGDVARHLDFSAAEQPVA
LQLGGSEPVDLAQAAKLGEQWGYKEINLNCGCPSERVQRGAFGACLMAEPQLVADCVRAMRDVVSVPVTVKHRIGIDTIE
HYGFVRDFVGTIAEAGCDIFIVHARNAILKGLSPKENREIPPLRYEVAYELKREFPHLEILINGGVVSYGEIDEHLRHVD
GVMIGRQAYHDPYFLSEMDARFYGGTAPIVTREMAELTMQAYIGDLVEKGGYMGAATRHMLGLHRGIYGGRGWRRVLSDA
RRMNAARSRADVDALFEEARSHLRPGLQEAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=236, Percent_Identity=26.6949152542373, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI40807366, Length=247, Percent_Identity=27.5303643724696, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI145693211, Length=313, Percent_Identity=57.5079872204473, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1789660, Length=241, Percent_Identity=24.896265560166, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788462, Length=243, Percent_Identity=26.3374485596708, Blast_Score=69, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=239, Percent_Identity=27.1966527196653, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24580595, Length=255, Percent_Identity=25.8823529411765, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI19920448, Length=255, Percent_Identity=25.8823529411765, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 39098; Mature: 39098

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQTSSTPQTESRGGSQGNIRPRRISVAPMMDWTDRHCRTFHRALSQHTWLYTEMVTTGA
CCCCCCCCCHHHCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LLHGDVARHLDFSAAEQPVALQLGGSEPVDLAQAAKLGEQWGYKEINLNCGCPSERVQRG
HHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHCC
AFGACLMAEPQLVADCVRAMRDVVSVPVTVKHRIGIDTIEHYGFVRDFVGTIAEAGCDIF
CCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEHHCCCHHHHHHHHHHHHHHHHHHCCCCEE
IVHARNAILKGLSPKENREIPPLRYEVAYELKREFPHLEILINGGVVSYGEIDEHLRHVD
EEECCHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCEEECHHHHHHHHHHH
GVMIGRQAYHDPYFLSEMDARFYGGTAPIVTREMAELTMQAYIGDLVEKGGYMGAATRHM
HHHCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LGLHRGIYGGRGWRRVLSDARRMNAARSRADVDALFEEARSHLRPGLQEAA
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCC
>Mature Secondary Structure
MEQTSSTPQTESRGGSQGNIRPRRISVAPMMDWTDRHCRTFHRALSQHTWLYTEMVTTGA
CCCCCCCCCHHHCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LLHGDVARHLDFSAAEQPVALQLGGSEPVDLAQAAKLGEQWGYKEINLNCGCPSERVQRG
HHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHCC
AFGACLMAEPQLVADCVRAMRDVVSVPVTVKHRIGIDTIEHYGFVRDFVGTIAEAGCDIF
CCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEHHCCCHHHHHHHHHHHHHHHHHHCCCCEE
IVHARNAILKGLSPKENREIPPLRYEVAYELKREFPHLEILINGGVVSYGEIDEHLRHVD
EEECCHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCEEECHHHHHHHHHHH
GVMIGRQAYHDPYFLSEMDARFYGGTAPIVTREMAELTMQAYIGDLVEKGGYMGAATRHM
HHHCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LGLHRGIYGGRGWRRVLSDARRMNAARSRADVDALFEEARSHLRPGLQEAA
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]