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The map label for this gene is ansB [H]

Identifier: 94310918

GI number: 94310918

Start: 2154051

End: 2155052

Strand: Reverse

Name: ansB [H]

Synonym: Rmet_1980

Alternate gene names: 94310918

Gene position: 2155052-2154051 (Counterclockwise)

Preceding gene: 94310921

Following gene: 94310917

Centisome position: 54.86

GC content: 65.57

Gene sequence:

>1002_bases
ATGTCCGTACTCCTGCCTCGTATCGTTGTGCTCGCGACTGGTGGCACCATCGCCGGTTCGTCCGCAAATCCTGCCAGTAG
CGCGCACTATCAGGCTGCGACAGTGCCCGTATCGCAACTTGTACAGGCAGTGCCCGCGCTGGCCGATGTGGCGCGAGTCG
AGGCGGAGCAGGTGGCGCAGGTGGACAGCAAGGACATGATGTTCGATCTCTGGCGGATGCTTGCCGCGCGAATCGGGTAT
TGGTCCGCGCAGGACGATGTCGCCGGGATCGTGATCACACATGGCACTGACACGCTGGAAGAGACGGCGATGTTCCTGCA
TCTGACACAGGCTTGCCCGTTGCCTGTCGTGATGACGGCGGCCATGCGCCCATCGACGTCGCTTTCCGCTGACGGGCCGC
TGAACCTGCTCGATGCGGTGCGCGTGGCGGCAAGCCCGGTGGCAGTTGGCAAAGGTGCGCTCGTGGTGCTGAATCAGCAG
ATTCATGCCGCGCGTGATGTCGTCAAGGCGCATACGTCGGCTGTCGATGCTTTCCATTCGCCAGTTGCCGGTCCGCTGGG
TTTTGTGCAGGACACCTATGTCCATTTCTCGCGCACCCCGGGTCCTGATCAGGCGCCGCGCTTGCCGGTGCCCGCTGCAT
GGCCGTTGGTGGAGATCGTCGCCAGCTACGCGCAGCCGGGGAGGGTGGGTATCGATGCGATGGTGGCCGCCGGCGTGCTC
GGGTTTGTCGTGGCCGCGGCCGGCAACGGTTCCGTGCACGAAACGCTGACGGCCGCGCTGGTCGATGCTGCGGCATGTGG
CGTTGCGGTGGCTCGAAGCTCGCGTACCGGTGCTGGTCATGTGGCAATCCCCGCACCGCCGCGACCGGCAGGTGGCGTAT
TTGTCTCGGCGGGCGACCTCAATCCCTACAAAGCGCGCGTGCTGCTGCTGCTGGCGTTGGCCGGCGACCCTTCGCTGGGG
CGCGATGCGGCCCGGCTTCAGGCGCTCTTCGAGCGGTACTGA

Upstream 100 bases:

>100_bases
GAGTAGTTCTGTGGGGTGAGGCCCACGGGTTTGTCACGGGTTCGGCCAGGCGTTCGCGCAGTTACAATCGGCCCGCCTAC
AAGCTCCGTCAACCCCGTCC

Downstream 100 bases:

>100_bases
GACAGGCCGGGTGCCGAATCCTTGCGGGAAGGCCCCTTTCCGGGCTATACTCGCGGGCTACTCAACCTTGCCGCACCGGA
CGTGACGCCCGGGTGGCTCA

Product: periplasmic L-asparaginase II

Products: NA

Alternate protein names: L-ASNase 2; L-asparagine amidohydrolase 2 [H]

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MSVLLPRIVVLATGGTIAGSSANPASSAHYQAATVPVSQLVQAVPALADVARVEAEQVAQVDSKDMMFDLWRMLAARIGY
WSAQDDVAGIVITHGTDTLEETAMFLHLTQACPLPVVMTAAMRPSTSLSADGPLNLLDAVRVAASPVAVGKGALVVLNQQ
IHAARDVVKAHTSAVDAFHSPVAGPLGFVQDTYVHFSRTPGPDQAPRLPVPAAWPLVEIVASYAQPGRVGIDAMVAAGVL
GFVVAAAGNGSVHETLTAALVDAAACGVAVARSSRTGAGHVAIPAPPRPAGGVFVSAGDLNPYKARVLLLLALAGDPSLG
RDAARLQALFERY

Sequences:

>Translated_333_residues
MSVLLPRIVVLATGGTIAGSSANPASSAHYQAATVPVSQLVQAVPALADVARVEAEQVAQVDSKDMMFDLWRMLAARIGY
WSAQDDVAGIVITHGTDTLEETAMFLHLTQACPLPVVMTAAMRPSTSLSADGPLNLLDAVRVAASPVAVGKGALVVLNQQ
IHAARDVVKAHTSAVDAFHSPVAGPLGFVQDTYVHFSRTPGPDQAPRLPVPAAWPLVEIVASYAQPGRVGIDAMVAAGVL
GFVVAAAGNGSVHETLTAALVDAAACGVAVARSSRTGAGHVAIPAPPRPAGGVFVSAGDLNPYKARVLLLLALAGDPSLG
RDAARLQALFERY
>Mature_332_residues
SVLLPRIVVLATGGTIAGSSANPASSAHYQAATVPVSQLVQAVPALADVARVEAEQVAQVDSKDMMFDLWRMLAARIGYW
SAQDDVAGIVITHGTDTLEETAMFLHLTQACPLPVVMTAAMRPSTSLSADGPLNLLDAVRVAASPVAVGKGALVVLNQQI
HAARDVVKAHTSAVDAFHSPVAGPLGFVQDTYVHFSRTPGPDQAPRLPVPAAWPLVEIVASYAQPGRVGIDAMVAAGVLG
FVVAAAGNGSVHETLTAALVDAAACGVAVARSSRTGAGHVAIPAPPRPAGGVFVSAGDLNPYKARVLLLLALAGDPSLGR
DAARLQALFERY

Specific function: Catalyzes the conversion of L-asparagine to L-aspartate and ammonium [H]

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=333, Percent_Identity=43.2432432432432, Blast_Score=235, Evalue=4e-63,
Organism=Escherichia coli, GI1788065, Length=296, Percent_Identity=29.0540540540541, Blast_Score=74, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323189, Length=320, Percent_Identity=36.875, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6323187, Length=320, Percent_Identity=36.875, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6323186, Length=320, Percent_Identity=36.875, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6323184, Length=320, Percent_Identity=36.875, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6320527, Length=324, Percent_Identity=35.8024691358025, Blast_Score=167, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 34173; Mature: 34041

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLLPRIVVLATGGTIAGSSANPASSAHYQAATVPVSQLVQAVPALADVARVEAEQVAQ
CCCHHCEEEEEECCCEEECCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
VDSKDMMFDLWRMLAARIGYWSAQDDVAGIVITHGTDTLEETAMFLHLTQACPLPVVMTA
CCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHH
AMRPSTSLSADGPLNLLDAVRVAASPVAVGKGALVVLNQQIHAARDVVKAHTSAVDAFHS
HCCCCCCCCCCCCHHHHHHHHHHCCCCEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHC
PVAGPLGFVQDTYVHFSRTPGPDQAPRLPVPAAWPLVEIVASYAQPGRVGIDAMVAAGVL
CCCCCHHHHHHHHEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHH
GFVVAAAGNGSVHETLTAALVDAAACGVAVARSSRTGAGHVAIPAPPRPAGGVFVSAGDL
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEECCCC
NPYKARVLLLLALAGDPSLGRDAARLQALFERY
CHHHHHHHHHEEECCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SVLLPRIVVLATGGTIAGSSANPASSAHYQAATVPVSQLVQAVPALADVARVEAEQVAQ
CCHHCEEEEEECCCEEECCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
VDSKDMMFDLWRMLAARIGYWSAQDDVAGIVITHGTDTLEETAMFLHLTQACPLPVVMTA
CCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHH
AMRPSTSLSADGPLNLLDAVRVAASPVAVGKGALVVLNQQIHAARDVVKAHTSAVDAFHS
HCCCCCCCCCCCCHHHHHHHHHHCCCCEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHC
PVAGPLGFVQDTYVHFSRTPGPDQAPRLPVPAAWPLVEIVASYAQPGRVGIDAMVAAGVL
CCCCCHHHHHHHHEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHH
GFVVAAAGNGSVHETLTAALVDAAACGVAVARSSRTGAGHVAIPAPPRPAGGVFVSAGDL
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEECCCC
NPYKARVLLLLALAGDPSLGRDAARLQALFERY
CHHHHHHHHHEEECCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377 [H]