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The map label for this gene is pitA [H]

Identifier: 94310911

GI number: 94310911

Start: 2149161

End: 2150171

Strand: Reverse

Name: pitA [H]

Synonym: Rmet_1973

Alternate gene names: 94310911

Gene position: 2150171-2149161 (Counterclockwise)

Preceding gene: 94310912

Following gene: 335055567

Centisome position: 54.74

GC content: 61.62

Gene sequence:

>1011_bases
ATGCATACTGTTCAAATGAGCTTGTGGGTGATCGGCCTGTTGGTCGCGCTTGCGCTGCTGTTCGACTTCATGAACGGCTT
CCACGACGCGGCCAACTCGATCGCCACGGTGGTTTCCACGGGCGTGCTCAAGCCGCACCATGCCGTGGCCATGGCGGCCA
TGTGCAACGTGATCGCCATCTTTATTTTCCACCTCAAGGTGGCAGCCACTGTCGGTACCGGAACCATCGACGTCAACATC
GTCGATCATTACGTGATTTTCGGTGCCTTGGTTGGGGCGATTGCCTGGAACGTGATTACCTGGCTGTATGGCATCCCTTC
GTCGTCCTCGCACGCGCTGATCGGCGGGCTGGTGGGTGCCGCGGTGGCCAAGGCAGGCACGGGCGCCCTGGTCGGCAACG
GCCTGCTCAAGACCGTCGCGTTTATCCTGATCTCGCCGCTGCTCGGCTTCATCCTGGGTTCGATCATGATGGTGATCGTC
GGCTGGACGTTCTTCCGCACGCCGCCGTCGCGCGTGGATCGCTGGTTCCGTCGCCTGCAACTGGTGTCGGCGTCGCTCTA
CAGCCTGGGTCATGGCGGTAATGACGCGCAGAAGACCATCGGCATTATCTGGATGCTGTTGATTGCCAGCGGTCATGTCG
CAGTGGGTGGTGCCGCGCCGCCGATGTGGGTGATTGTGAGCTGCTACCTGGCCATCGGCATGGGCACGCTGTTCGGCGGT
TGGCGGATTGTGCGCACGATGGGTCAGAAGATCACCAAGCTGAAGCCCGTGGGTGGCTTCTGTGCGGAGACAGGCGGTGC
GATCACGCTGTTCATTGCTTCCGCGCTGGGTGTGCCGGTGTCGACCACACATACGATCACCGGTGCGATCGTCGGCGTTG
GCTCGGCTCAGAAGATGTCGGCCGTGCGCTGGGGCGTGGCGGGCAACATCGTGTGGGCCTGGGTCCTGACGATTCCGGCC
TCGGCATTTATGTCCGCCATTGCCTGGTGGATCGGTAAGCAGATTCTGTAA

Upstream 100 bases:

>100_bases
GCGATCTACGAACAGCTCGAGTCCATTACCGACAAGTGCGAGGACGTGGCCAACATCATCGAAGGCATCGTCCTGGAAAA
CGCCTGAGGCGCGTACTCGC

Downstream 100 bases:

>100_bases
CGCTGGCAAGCGCGAGCGGAATCCCAATGACAAGCCAGGCTTCGTGCCTGGCTTGTTCGTTTACCGGTTCGCGAATGCGG
CGATCGGATCGTCGTCGTCC

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MHTVQMSLWVIGLLVALALLFDFMNGFHDAANSIATVVSTGVLKPHHAVAMAAMCNVIAIFIFHLKVAATVGTGTIDVNI
VDHYVIFGALVGAIAWNVITWLYGIPSSSSHALIGGLVGAAVAKAGTGALVGNGLLKTVAFILISPLLGFILGSIMMVIV
GWTFFRTPPSRVDRWFRRLQLVSASLYSLGHGGNDAQKTIGIIWMLLIASGHVAVGGAAPPMWVIVSCYLAIGMGTLFGG
WRIVRTMGQKITKLKPVGGFCAETGGAITLFIASALGVPVSTTHTITGAIVGVGSAQKMSAVRWGVAGNIVWAWVLTIPA
SAFMSAIAWWIGKQIL

Sequences:

>Translated_336_residues
MHTVQMSLWVIGLLVALALLFDFMNGFHDAANSIATVVSTGVLKPHHAVAMAAMCNVIAIFIFHLKVAATVGTGTIDVNI
VDHYVIFGALVGAIAWNVITWLYGIPSSSSHALIGGLVGAAVAKAGTGALVGNGLLKTVAFILISPLLGFILGSIMMVIV
GWTFFRTPPSRVDRWFRRLQLVSASLYSLGHGGNDAQKTIGIIWMLLIASGHVAVGGAAPPMWVIVSCYLAIGMGTLFGG
WRIVRTMGQKITKLKPVGGFCAETGGAITLFIASALGVPVSTTHTITGAIVGVGSAQKMSAVRWGVAGNIVWAWVLTIPA
SAFMSAIAWWIGKQIL
>Mature_336_residues
MHTVQMSLWVIGLLVALALLFDFMNGFHDAANSIATVVSTGVLKPHHAVAMAAMCNVIAIFIFHLKVAATVGTGTIDVNI
VDHYVIFGALVGAIAWNVITWLYGIPSSSSHALIGGLVGAAVAKAGTGALVGNGLLKTVAFILISPLLGFILGSIMMVIV
GWTFFRTPPSRVDRWFRRLQLVSASLYSLGHGGNDAQKTIGIIWMLLIASGHVAVGGAAPPMWVIVSCYLAIGMGTLFGG
WRIVRTMGQKITKLKPVGGFCAETGGAITLFIASALGVPVSTTHTITGAIVGVGSAQKMSAVRWGVAGNIVWAWVLTIPA
SAFMSAIAWWIGKQIL

Specific function: Low-affinity inorganic phosphate transport (Probable) [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=164, Percent_Identity=35.3658536585366, Blast_Score=98, Evalue=9e-21,
Organism=Homo sapiens, GI5803173, Length=154, Percent_Identity=37.012987012987, Blast_Score=95, Evalue=8e-20,
Organism=Escherichia coli, GI1789360, Length=218, Percent_Identity=32.1100917431193, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1789907, Length=218, Percent_Identity=32.5688073394495, Blast_Score=92, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17539280, Length=167, Percent_Identity=32.9341317365269, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI25146401, Length=162, Percent_Identity=31.4814814814815, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71981576, Length=162, Percent_Identity=29.0123456790123, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI32566716, Length=168, Percent_Identity=30.3571428571429, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17536725, Length=189, Percent_Identity=29.6296296296296, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17557328, Length=163, Percent_Identity=34.3558282208589, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319773, Length=160, Percent_Identity=30, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21356511, Length=187, Percent_Identity=31.0160427807487, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 35203; Mature: 35203

Theoretical pI: Translated: 10.60; Mature: 10.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9696772; 11481430 [H]