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The map label for this gene is nolF [H]

Identifier: 94310881

GI number: 94310881

Start: 2113012

End: 2114583

Strand: Reverse

Name: nolF [H]

Synonym: Rmet_1943

Alternate gene names: 94310881

Gene position: 2114583-2113012 (Counterclockwise)

Preceding gene: 94310884

Following gene: 94310880

Centisome position: 53.83

GC content: 66.79

Gene sequence:

>1572_bases
ATGTGGGGCGGCGGGGGCGGCGCCAAAGGCGCGGATTGTAGCACCGGGCGAAGCCGCCGGCGCCTTCAGGCGCCCGCTGA
CGCTCGCCTGCGGACCTTTCGTCGCACGATACTCCTGTTCGTCATCCGCCGTTTGACGCACTCGAGGGTGATCAGTATAT
TACTGACCGGCCAGTTATTAATTTCTTCCCTTGGCCCCAGGCATCCGCGCAGATGCCTGAAAGTTATCCCAAAGACGCAT
TCCAGAGAGTCGCTCGCCGTCAGGCGAGGGCCACCGGCTGCACCCGTTCCACCGACCAGATATCGCTTGTCCATGCCAGT
GCATTACGCCGGGCCGGAAGTTGCGGCCGTAGACGCTGAAGTTCGTTCTCTCGCGCCGCTGCCGGAACGTCGGCGCGGAC
GCCGTGCCGCCCGGCCCATCGCCGCGGGGCTGGCCATGCTTGCGCTTTCGACTGCCTTGATGCTGGCTGGCTGCGGCAAG
CAGGCGGAATCGGTCGAATACGTGCGGCCGGTGCGGTTGCTGCAACTGAACCCGGCAGGGGGCAAGACGGCCTTTGAATT
CTCCGGCGAGGTGCGCCCGCGCGTGGAGTCGCGCCTGGGTTTCCGCGTGGGCGGAAAGATCGCCGCCCGTCTGGTGGACG
TGGGTGCCACTGTGCGCAAGGGTCAGCCGCTGGCCCGCCTTGATCCGACCGACCTGGGGCTGGCGGAAGCCGGCGCGCGT
GCCCAGTACGACGCGGCCAAGACCGACCGCGATCTCGCGGCGTCCGATCTCAAGCGCTACAACGAACTCGCGGCCAAGGG
ATTTATCAGTGCCGCCGAGCAGCACCGCCGCCAGGCCACCTACGATTCGGCCGCTTCGAAGCTGGAGCAGGCCCAGGCCA
ATCTGCGCAATCAGGCGAACCAGACCGGCTATGCGGTGCTGACCGCCGACGCCGATGGCGTGGTGACCGCGATCGACACC
GAGGTGGGGCAGGTCGTTACACCGGGCCAGCCGGTGATCCGTGTGGCGCAGACGGCCGAGAAGGAAGTGGCGATCGGCCT
GCCGGAAGATCAGGTCGACATGCTGCGTGGCATTACCGAAGTCACGATTCGCACCTGGTCGGAACCGGATCGCGTGCTGC
CGGGACGGGTTCGCGAGATTTCCGCAGCTGCCGATACGGTGACGCGGACGTACCCGACGCGTGTCAGCGTGCCCAATCCG
CCGGCTGACCTGCGCATCGGCATGACCGCCGTGGTGACCTTTGTCCGATCTGGCGACAGTGCCGCGATTCGCATTCCGCT
GACGGCCCTGCTGCAGAACAACGGCAGCAATCAGGTCTGGGTCTATCAACCGGATTCCGGCGGCAAGGGCACGGGTGGCA
CGGTCAACCCGGTGCCGGTGATGCTCGGCAACGCCGACGGCAACCTGATAGAAGTGCGAAGCGGTCTGAAGCCGGGTCAG
GTCATCGTCACGGCGGGTGTGCACCTGCTCAGGCCGGGGCAGAAGGTCAGGCCGATGCAGAGCATCGCTCCGGCGCAGCC
GGCCTCGAATCCTGGCGCGCCGCTGGCCGCGCTGGATAGGCGCCTGGGCTGA

Upstream 100 bases:

>100_bases
CGGTCGGAGGGGAGAAGCAGGCTACTGTAGTAGAAGCTGGAGCCGCTCTGGGCGGCCTTGTCCTGGCAATACTGATCGGG
CGTCACGCCAATCTCTTTGA

Downstream 100 bases:

>100_bases
GGGCGGCGCATGGACCATTCCCGCTTCAACCTGTCGCGCTGGGCGCTTGAGCACCAGCCGCTGACCCGCTACCTGCTCGT
TGTGCTGCTGCTGGGCGGCC

Product: cation/multidrug efflux system, mebrane-fusion component (RND family)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MWGGGGGAKGADCSTGRSRRRLQAPADARLRTFRRTILLFVIRRLTHSRVISILLTGQLLISSLGPRHPRRCLKVIPKTH
SRESLAVRRGPPAAPVPPTRYRLSMPVHYAGPEVAAVDAEVRSLAPLPERRRGRRAARPIAAGLAMLALSTALMLAGCGK
QAESVEYVRPVRLLQLNPAGGKTAFEFSGEVRPRVESRLGFRVGGKIAARLVDVGATVRKGQPLARLDPTDLGLAEAGAR
AQYDAAKTDRDLAASDLKRYNELAAKGFISAAEQHRRQATYDSAASKLEQAQANLRNQANQTGYAVLTADADGVVTAIDT
EVGQVVTPGQPVIRVAQTAEKEVAIGLPEDQVDMLRGITEVTIRTWSEPDRVLPGRVREISAAADTVTRTYPTRVSVPNP
PADLRIGMTAVVTFVRSGDSAAIRIPLTALLQNNGSNQVWVYQPDSGGKGTGGTVNPVPVMLGNADGNLIEVRSGLKPGQ
VIVTAGVHLLRPGQKVRPMQSIAPAQPASNPGAPLAALDRRLG

Sequences:

>Translated_523_residues
MWGGGGGAKGADCSTGRSRRRLQAPADARLRTFRRTILLFVIRRLTHSRVISILLTGQLLISSLGPRHPRRCLKVIPKTH
SRESLAVRRGPPAAPVPPTRYRLSMPVHYAGPEVAAVDAEVRSLAPLPERRRGRRAARPIAAGLAMLALSTALMLAGCGK
QAESVEYVRPVRLLQLNPAGGKTAFEFSGEVRPRVESRLGFRVGGKIAARLVDVGATVRKGQPLARLDPTDLGLAEAGAR
AQYDAAKTDRDLAASDLKRYNELAAKGFISAAEQHRRQATYDSAASKLEQAQANLRNQANQTGYAVLTADADGVVTAIDT
EVGQVVTPGQPVIRVAQTAEKEVAIGLPEDQVDMLRGITEVTIRTWSEPDRVLPGRVREISAAADTVTRTYPTRVSVPNP
PADLRIGMTAVVTFVRSGDSAAIRIPLTALLQNNGSNQVWVYQPDSGGKGTGGTVNPVPVMLGNADGNLIEVRSGLKPGQ
VIVTAGVHLLRPGQKVRPMQSIAPAQPASNPGAPLAALDRRLG
>Mature_523_residues
MWGGGGGAKGADCSTGRSRRRLQAPADARLRTFRRTILLFVIRRLTHSRVISILLTGQLLISSLGPRHPRRCLKVIPKTH
SRESLAVRRGPPAAPVPPTRYRLSMPVHYAGPEVAAVDAEVRSLAPLPERRRGRRAARPIAAGLAMLALSTALMLAGCGK
QAESVEYVRPVRLLQLNPAGGKTAFEFSGEVRPRVESRLGFRVGGKIAARLVDVGATVRKGQPLARLDPTDLGLAEAGAR
AQYDAAKTDRDLAASDLKRYNELAAKGFISAAEQHRRQATYDSAASKLEQAQANLRNQANQTGYAVLTADADGVVTAIDT
EVGQVVTPGQPVIRVAQTAEKEVAIGLPEDQVDMLRGITEVTIRTWSEPDRVLPGRVREISAAADTVTRTYPTRVSVPNP
PADLRIGMTAVVTFVRSGDSAAIRIPLTALLQNNGSNQVWVYQPDSGGKGTGGTVNPVPVMLGNADGNLIEVRSGLKPGQ
VIVTAGVHLLRPGQKVRPMQSIAPAQPASNPGAPLAALDRRLG

Specific function: Involved in the production of Medicago-specific nodulation signal molecule [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87082036, Length=318, Percent_Identity=24.5283018867925, Blast_Score=75, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 55727; Mature: 55727

Theoretical pI: Translated: 11.35; Mature: 11.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWGGGGGAKGADCSTGRSRRRLQAPADARLRTFRRTILLFVIRRLTHSRVISILLTGQLL
CCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISSLGPRHPRRCLKVIPKTHSRESLAVRRGPPAAPVPPTRYRLSMPVHYAGPEVAAVDAE
HHHCCCCCHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCEEEECCEEECCCCEEEECHH
VRSLAPLPERRRGRRAARPIAAGLAMLALSTALMLAGCGKQAESVEYVRPVRLLQLNPAG
HHHCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCEEEEEECCCC
GKTAFEFSGEVRPRVESRLGFRVGGKIAARLVDVGATVRKGQPLARLDPTDLGLAEAGAR
CCEEEEECCCCCCHHHHHCCCCCCHHHHHHHHHHCCHHHCCCCCCCCCCCCCCHHHCCCC
AQYDAAKTDRDLAASDLKRYNELAAKGFISAAEQHRRQATYDSAASKLEQAQANLRNQAN
CCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QTGYAVLTADADGVVTAIDTEVGQVVTPGQPVIRVAQTAEKEVAIGLPEDQVDMLRGITE
CCCEEEEEECCCCEEEEEECCCCCEECCCCHHHHHHHHCCCCEEECCCHHHHHHHHCHHH
VTIRTWSEPDRVLPGRVREISAAADTVTRTYPTRVSVPNPPADLRIGMTAVVTFVRSGDS
EEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCEECHHHHHHHHHCCCC
AAIRIPLTALLQNNGSNQVWVYQPDSGGKGTGGTVNPVPVMLGNADGNLIEVRSGLKPGQ
CEEEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCE
VIVTAGVHLLRPGQKVRPMQSIAPAQPASNPGAPLAALDRRLG
EEEECCHHHCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHCCC
>Mature Secondary Structure
MWGGGGGAKGADCSTGRSRRRLQAPADARLRTFRRTILLFVIRRLTHSRVISILLTGQLL
CCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISSLGPRHPRRCLKVIPKTHSRESLAVRRGPPAAPVPPTRYRLSMPVHYAGPEVAAVDAE
HHHCCCCCHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCEEEECCEEECCCCEEEECHH
VRSLAPLPERRRGRRAARPIAAGLAMLALSTALMLAGCGKQAESVEYVRPVRLLQLNPAG
HHHCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCEEEEEECCCC
GKTAFEFSGEVRPRVESRLGFRVGGKIAARLVDVGATVRKGQPLARLDPTDLGLAEAGAR
CCEEEEECCCCCCHHHHHCCCCCCHHHHHHHHHHCCHHHCCCCCCCCCCCCCCHHHCCCC
AQYDAAKTDRDLAASDLKRYNELAAKGFISAAEQHRRQATYDSAASKLEQAQANLRNQAN
CCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QTGYAVLTADADGVVTAIDTEVGQVVTPGQPVIRVAQTAEKEVAIGLPEDQVDMLRGITE
CCCEEEEEECCCCEEEEEECCCCCEECCCCHHHHHHHHCCCCEEECCCHHHHHHHHCHHH
VTIRTWSEPDRVLPGRVREISAAADTVTRTYPTRVSVPNPPADLRIGMTAVVTFVRSGDS
EEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCEECHHHHHHHHHCCCC
AAIRIPLTALLQNNGSNQVWVYQPDSGGKGTGGTVNPVPVMLGNADGNLIEVRSGLKPGQ
CEEEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCE
VIVTAGVHLLRPGQKVRPMQSIAPAQPASNPGAPLAALDRRLG
EEEECCHHHCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1909418; 11481432 [H]