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The map label for this gene is gatA3 [H]

Identifier: 94310865

GI number: 94310865

Start: 2095100

End: 2096422

Strand: Reverse

Name: gatA3 [H]

Synonym: Rmet_1927

Alternate gene names: 94310865

Gene position: 2096422-2095100 (Counterclockwise)

Preceding gene: 94310866

Following gene: 94310864

Centisome position: 53.37

GC content: 68.48

Gene sequence:

>1323_bases
ATGAGCGCGCCGACCATTGCCCAACTGGCCGCAGCGCTGGCTGCAGGCCGCACCACGAGCGTGGGGCTGACCGAGGCCGC
GCTGGCTAATATCGCCCGGCATCAGCAGGCGGGCGGATCGGCCTTCATGATGGTCGATGCAGAAGGCGCGCTGGCCCAGG
CACGTGCGGCCGATCAGGCGCGCAGCGCGGGCCTTGTGGCCTCGCCGCTGGCCGGGCTGCCGATCTCGATCAAGGACCTG
TTCGACGTTCGCGGTCAGGTCACGCGTGCCGGATCGAAGGCCCTCGACGGCGGTGCGCCGGCGCAGGCTGACTCGCCCGC
CGTGGCGCGACTACGTGCCGCTGGCGCGGTGCTGATCGGCCGTACCAATATGAGCGAGTTCGCGTTCTCGGGCCTGGGGC
TGAATCCGCACTACGGCACGCCGCGCACGCCGTTCGACCCCGAGCGTGTTGCCGGTGGATCGACATCGGGTGGTGCGGTG
AGCGTGGCCGAAGACATGGCTGTCGCTGCGCTCGGCACCGATACCGGCGGCTCGATCCGCATTCCGTCGGCATTCTGCGG
CTTGACTGGTTTCAAGCCGACGGCGCGTCGCGTGCCGCTGGACGGCGCAGTGCCGTTGTCGACGTCGCTCGACTCCGCAG
GGCCGCTGGCGCGCTCGGTTGCCTGTTGCGTGGCGATGGATGCGGTGCTGAGCGGCGAGACGCTGGATACCCGCGCGGCG
GACCTGCGTGGACTGCGCTTCTACGTGACGCGCGATTTCGTCTGCGATGGTCTCGACCCGGAAGTGGCGCAGACGTTCGA
AGCCGCACTGGCAAGGCTGTCAGCCCAGGGCGCCACGATCGTTCCATTCGACTTCCCCGAACTGCGCCGCCTGCCGGAGA
TCAATGGCGGTGGAGGCCTGACCGCGGCCGAGTCGTGGGCCTGGCATCGCACATTGCTGGCGGCCAAGGGTGACCAGTAC
GACCAACGAGTGGCACAGCGCATTCGCCGGGGCGAGAAGCAGTCGGCGGCGGATTACATCGACTTGCTGGCCGCGCGATG
CGCGATGCAACAGCGTGCCGCCGTCTTGCTGCGTGACGCCGATGCATGGCTGATGCCGACCGTGGCCGTACGCCCGCCGC
GTCTCGATGCCCTCGAACGCGACGAAGACTTCTTCGCGGTCAACGGCCTGGTGCTTCGCAATGCAAGCGTGATCAATTTC
CTCGACGGCTGTGCGGCGACACTGCCAGCCGGCGAAGGTATTGGCCTCGGCGTGTGCGGCCTGCATGGCAGTGACGCGCG
CGTGTTGCAGGTGGCCGCGGCCATCGAACGCGCCTTCGATTGA

Upstream 100 bases:

>100_bases
AACTGGGCCATGGCGAAGCGTTCGAAGTGGAACTGCACGACCCCGTGCGCAACCGCACGCTGCGCCATGCATATCGCGTG
GAATCACTGGCGGTGGCCGA

Downstream 100 bases:

>100_bases
TGCGCTGATCCTTCTTTCCGCCGGATCCCACGGCTAGCACAACGCAAGACCGAGAGGGCCGCGCCAACGCAGCGCGGCCC
CGCCCACCACACCCAAGGAG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MSAPTIAQLAAALAAGRTTSVGLTEAALANIARHQQAGGSAFMMVDAEGALAQARAADQARSAGLVASPLAGLPISIKDL
FDVRGQVTRAGSKALDGGAPAQADSPAVARLRAAGAVLIGRTNMSEFAFSGLGLNPHYGTPRTPFDPERVAGGSTSGGAV
SVAEDMAVAALGTDTGGSIRIPSAFCGLTGFKPTARRVPLDGAVPLSTSLDSAGPLARSVACCVAMDAVLSGETLDTRAA
DLRGLRFYVTRDFVCDGLDPEVAQTFEAALARLSAQGATIVPFDFPELRRLPEINGGGGLTAAESWAWHRTLLAAKGDQY
DQRVAQRIRRGEKQSAADYIDLLAARCAMQQRAAVLLRDADAWLMPTVAVRPPRLDALERDEDFFAVNGLVLRNASVINF
LDGCAATLPAGEGIGLGVCGLHGSDARVLQVAAAIERAFD

Sequences:

>Translated_440_residues
MSAPTIAQLAAALAAGRTTSVGLTEAALANIARHQQAGGSAFMMVDAEGALAQARAADQARSAGLVASPLAGLPISIKDL
FDVRGQVTRAGSKALDGGAPAQADSPAVARLRAAGAVLIGRTNMSEFAFSGLGLNPHYGTPRTPFDPERVAGGSTSGGAV
SVAEDMAVAALGTDTGGSIRIPSAFCGLTGFKPTARRVPLDGAVPLSTSLDSAGPLARSVACCVAMDAVLSGETLDTRAA
DLRGLRFYVTRDFVCDGLDPEVAQTFEAALARLSAQGATIVPFDFPELRRLPEINGGGGLTAAESWAWHRTLLAAKGDQY
DQRVAQRIRRGEKQSAADYIDLLAARCAMQQRAAVLLRDADAWLMPTVAVRPPRLDALERDEDFFAVNGLVLRNASVINF
LDGCAATLPAGEGIGLGVCGLHGSDARVLQVAAAIERAFD
>Mature_439_residues
SAPTIAQLAAALAAGRTTSVGLTEAALANIARHQQAGGSAFMMVDAEGALAQARAADQARSAGLVASPLAGLPISIKDLF
DVRGQVTRAGSKALDGGAPAQADSPAVARLRAAGAVLIGRTNMSEFAFSGLGLNPHYGTPRTPFDPERVAGGSTSGGAVS
VAEDMAVAALGTDTGGSIRIPSAFCGLTGFKPTARRVPLDGAVPLSTSLDSAGPLARSVACCVAMDAVLSGETLDTRAAD
LRGLRFYVTRDFVCDGLDPEVAQTFEAALARLSAQGATIVPFDFPELRRLPEINGGGGLTAAESWAWHRTLLAAKGDQYD
QRVAQRIRRGEKQSAADYIDLLAARCAMQQRAAVLLRDADAWLMPTVAVRPPRLDALERDEDFFAVNGLVLRNASVINFL
DGCAATLPAGEGIGLGVCGLHGSDARVLQVAAAIERAFD

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=318, Percent_Identity=27.3584905660377, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI195972892, Length=247, Percent_Identity=30.3643724696356, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI166795287, Length=155, Percent_Identity=38.7096774193548, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17538252, Length=311, Percent_Identity=28.6173633440514, Blast_Score=101, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17543272, Length=177, Percent_Identity=36.1581920903955, Blast_Score=95, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=262, Percent_Identity=31.2977099236641, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17537465, Length=242, Percent_Identity=28.5123966942149, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17556264, Length=145, Percent_Identity=37.2413793103448, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17538254, Length=319, Percent_Identity=26.9592476489028, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=289, Percent_Identity=28.3737024221453, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=289, Percent_Identity=28.3737024221453, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6319685, Length=390, Percent_Identity=29.4871794871795, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323950, Length=421, Percent_Identity=24.4655581947743, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6320448, Length=226, Percent_Identity=30.5309734513274, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24648113, Length=305, Percent_Identity=33.1147540983607, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI45550774, Length=279, Percent_Identity=26.5232974910394, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648435, Length=279, Percent_Identity=26.5232974910394, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648437, Length=279, Percent_Identity=26.5232974910394, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648441, Length=219, Percent_Identity=26.9406392694064, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648439, Length=219, Percent_Identity=26.9406392694064, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24652985, Length=439, Percent_Identity=26.1958997722096, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI19922090, Length=439, Percent_Identity=26.1958997722096, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24652981, Length=439, Percent_Identity=26.1958997722096, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24652983, Length=439, Percent_Identity=26.1958997722096, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI21356731, Length=245, Percent_Identity=30.6122448979592, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24644968, Length=231, Percent_Identity=30.3030303030303, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI161078093, Length=417, Percent_Identity=24.4604316546763, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 45405; Mature: 45274

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPTIAQLAAALAAGRTTSVGLTEAALANIARHQQAGGSAFMMVDAEGALAQARAADQA
CCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHH
RSAGLVASPLAGLPISIKDLFDVRGQVTRAGSKALDGGAPAQADSPAVARLRAAGAVLIG
HHCCCEECCCCCCCCCHHHHHHHHHHHHHCCCHHCCCCCCCCCCCHHHHHHHHCCEEEEE
RTNMSEFAFSGLGLNPHYGTPRTPFDPERVAGGSTSGGAVSVAEDMAVAALGTDTGGSIR
CCCHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCCEEEHHHHHEEEEECCCCCCCEE
IPSAFCGLTGFKPTARRVPLDGAVPLSTSLDSAGPLARSVACCVAMDAVLSGETLDTRAA
CCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHH
DLRGLRFYVTRDFVCDGLDPEVAQTFEAALARLSAQGATIVPFDFPELRRLPEINGGGGL
HHCCEEEEEEECHHCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCHHHHCCCCCCCCCCC
TAAESWAWHRTLLAAKGDQYDQRVAQRIRRGEKQSAADYIDLLAARCAMQQRAAVLLRDA
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEECC
DAWLMPTVAVRPPRLDALERDEDFFAVNGLVLRNASVINFLDGCAATLPAGEGIGLGVCG
CCCCCCEEEECCCCCCCCCCCCCEEEECCEEEECCHHHHHHHHHHHHCCCCCCCCEEEEC
LHGSDARVLQVAAAIERAFD
CCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SAPTIAQLAAALAAGRTTSVGLTEAALANIARHQQAGGSAFMMVDAEGALAQARAADQA
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHH
RSAGLVASPLAGLPISIKDLFDVRGQVTRAGSKALDGGAPAQADSPAVARLRAAGAVLIG
HHCCCEECCCCCCCCCHHHHHHHHHHHHHCCCHHCCCCCCCCCCCHHHHHHHHCCEEEEE
RTNMSEFAFSGLGLNPHYGTPRTPFDPERVAGGSTSGGAVSVAEDMAVAALGTDTGGSIR
CCCHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCCEEEHHHHHEEEEECCCCCCCEE
IPSAFCGLTGFKPTARRVPLDGAVPLSTSLDSAGPLARSVACCVAMDAVLSGETLDTRAA
CCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHH
DLRGLRFYVTRDFVCDGLDPEVAQTFEAALARLSAQGATIVPFDFPELRRLPEINGGGGL
HHCCEEEEEEECHHCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCHHHHCCCCCCCCCCC
TAAESWAWHRTLLAAKGDQYDQRVAQRIRRGEKQSAADYIDLLAARCAMQQRAAVLLRDA
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEECC
DAWLMPTVAVRPPRLDALERDEDFFAVNGLVLRNASVINFLDGCAATLPAGEGIGLGVCG
CCCCCCEEEECCCCCCCCCCCCCEEEECCEEEECCHHHHHHHHHHHHCCCCCCCCEEEEC
LHGSDARVLQVAAAIERAFD
CCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA