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The map label for this gene is purT

Identifier: 94310758

GI number: 94310758

Start: 1975331

End: 1976533

Strand: Direct

Name: purT

Synonym: Rmet_1820

Alternate gene names: 94310758

Gene position: 1975331-1976533 (Clockwise)

Preceding gene: 94310757

Following gene: 94310761

Centisome position: 50.29

GC content: 67.0

Gene sequence:

>1203_bases
ATGACCACCCTTGGAACGCCCCTCTCCCCGTCCGCCACCAAAGTCATGCTGCTAGGCTCAGGTGAGCTGGGCAAGGAAGT
GCTGATCGCGCTGCAGCGCCTGGGCGTGGAGACGATCGCCGTCGACCGCTACGAGAACGCGCCGGGCCAGCAGGTGGCCC
ACCATGCGCGCACGATCACGATGAGCGACGGCGAACAGCTCAAGGCACTGATCGAAGCCGAGCGGCCGGATCTGGTGGTG
CCAGAGATCGAAGCCATCGCCACGCCAATGCTCGAAGCCCTGGAAGCGGCCGGCGTGGTTCGCGTGATCCCCACGGCTCG
CGCCGCCCGCCTGACGATGGACCGCGAAGGTATCCGCCGCCTGGCCGCCGAGACCCTGGGCCTGCCCACCAGCCCGTACA
AATTCTGCGATTCGCTCGAGGAACTGCAAGCCGCGATCGACGGCGGCATCGGCTACCCGTGCGTGGTCAAGCCCGTGATG
AGCAGTTCCGGCAAGGGCCAGAGCAAGATCGACGGTCCCGCGGACGTCAAGGCCGCGTGGGACTATGCCATGGCCGGCGG
CCGCGTCAGCCACGGCCGCGTGATCGTCGAAGGCTTTATCGACTTCGACTACGAAATCACGCTGCTGACCGTGCGCGCCG
TGGGCGCCAATGGCGAAGTGGAAACGCATTTCTGCGAGCCGATCGGCCACGTGCAGGTCGCGGGTGACTACGTGGAAAGC
TGGCAACCGCACCCGATGCACCCGAAGGCACTGGAAACCTCGCAGCATATCGCGCGTGCCGTGACCGCCGATCTGGGCGG
CCAGGGCCTGTTCGGCGTGGAACTGTTCGTCAAGGGCGAGCAGGTCTGGTTCAGCGAAGTCAGCCCGCGTCCGCACGACA
CCGGCATGGTGACGATGATTACGCAGTGGCAGAACGAGTTCGAACTCCACGCCCGCGCCATCCTCGGCCTGCCCGTCAGC
ACCGCGCTGCGCGCGCCCGGCGCGAGCGCCGTGATCTACGGCGGCGTTGAAGCCGAAGGCATTGTTTTCGATGGCGTGGA
CGAGGCGCTGCGCGTGCCGCAGACGGAACTGCGCCTGTTCGGCAAGCCGGAGTCGTTCACGAAGCGCCGCATGGGCGTGG
CGCTGGCCTACGCCGAAGACGTGGATACGGCACGCGAGCGCGCCAAGGAAGCGGCGGGCAAGGTCAAGCCGCGGAAGGCG
TAA

Upstream 100 bases:

>100_bases
TCGTACTGAGGAGCATAAGCGACGTCAGGGAACCGGGATGTCGCGCCGGGCATCGCCCGCGTATACAATCCCGGTTTTTC
TGACCTGATCGAGCCGCCCC

Downstream 100 bases:

>100_bases
GCGCTGCGCTTTGCTGACTCCCCTCTCCCGACGACGGGAGAAGGGAGAAGGGAGAAGGGACAGGGAGAAATCCCCCCCTA
AGCCACCCTGTCGGCCCGAT

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTTLGTPLSPSATKVMLLGSGELGKEVLIALQRLGVETIAVDRYENAPGQQVAHHARTITMSDGEQLKALIEAERPDLVV
PEIEAIATPMLEALEAAGVVRVIPTARAARLTMDREGIRRLAAETLGLPTSPYKFCDSLEELQAAIDGGIGYPCVVKPVM
SSSGKGQSKIDGPADVKAAWDYAMAGGRVSHGRVIVEGFIDFDYEITLLTVRAVGANGEVETHFCEPIGHVQVAGDYVES
WQPHPMHPKALETSQHIARAVTADLGGQGLFGVELFVKGEQVWFSEVSPRPHDTGMVTMITQWQNEFELHARAILGLPVS
TALRAPGASAVIYGGVEAEGIVFDGVDEALRVPQTELRLFGKPESFTKRRMGVALAYAEDVDTARERAKEAAGKVKPRKA

Sequences:

>Translated_400_residues
MTTLGTPLSPSATKVMLLGSGELGKEVLIALQRLGVETIAVDRYENAPGQQVAHHARTITMSDGEQLKALIEAERPDLVV
PEIEAIATPMLEALEAAGVVRVIPTARAARLTMDREGIRRLAAETLGLPTSPYKFCDSLEELQAAIDGGIGYPCVVKPVM
SSSGKGQSKIDGPADVKAAWDYAMAGGRVSHGRVIVEGFIDFDYEITLLTVRAVGANGEVETHFCEPIGHVQVAGDYVES
WQPHPMHPKALETSQHIARAVTADLGGQGLFGVELFVKGEQVWFSEVSPRPHDTGMVTMITQWQNEFELHARAILGLPVS
TALRAPGASAVIYGGVEAEGIVFDGVDEALRVPQTELRLFGKPESFTKRRMGVALAYAEDVDTARERAKEAAGKVKPRKA
>Mature_399_residues
TTLGTPLSPSATKVMLLGSGELGKEVLIALQRLGVETIAVDRYENAPGQQVAHHARTITMSDGEQLKALIEAERPDLVVP
EIEAIATPMLEALEAAGVVRVIPTARAARLTMDREGIRRLAAETLGLPTSPYKFCDSLEELQAAIDGGIGYPCVVKPVMS
SSGKGQSKIDGPADVKAAWDYAMAGGRVSHGRVIVEGFIDFDYEITLLTVRAVGANGEVETHFCEPIGHVQVAGDYVESW
QPHPMHPKALETSQHIARAVTADLGGQGLFGVELFVKGEQVWFSEVSPRPHDTGMVTMITQWQNEFELHARAILGLPVST
ALRAPGASAVIYGGVEAEGIVFDGVDEALRVPQTELRLFGKPESFTKRRMGVALAYAEDVDTARERAKEAAGKVKPRKA

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=397, Percent_Identity=59.6977329974811, Blast_Score=439, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6324702, Length=394, Percent_Identity=24.1116751269036, Blast_Score=96, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_RALME (Q1LMC7)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_583968.1
- ProteinModelPortal:   Q1LMC7
- SMR:   Q1LMC7
- STRING:   Q1LMC7
- GeneID:   4038622
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_1820
- eggNOG:   COG0027
- HOGENOM:   HBG516369
- OMA:   PDYIVPE
- PhylomeDB:   Q1LMC7
- ProtClustDB:   PRK09288
- BioCyc:   RMET266264:RMET_1820-MONOMER
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42967; Mature: 42835

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 82-82 BINDING 115-115 BINDING 157-157 BINDING 205-205 BINDING 274-274 BINDING 286-286 BINDING 293-293 BINDING 362-362

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLGTPLSPSATKVMLLGSGELGKEVLIALQRLGVETIAVDRYENAPGQQVAHHARTIT
CCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHEEEE
MSDGEQLKALIEAERPDLVVPEIEAIATPMLEALEAAGVVRVIPTARAARLTMDREGIRR
ECCCHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHCCEEEEECCCHHHHEECCHHHHHH
LAAETLGLPTSPYKFCDSLEELQAAIDGGIGYPCVVKPVMSSSGKGQSKIDGPADVKAAW
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHH
DYAMAGGRVSHGRVIVEGFIDFDYEITLLTVRAVGANGEVETHFCEPIGHVQVAGDYVES
HHHHCCCCCCCCEEEEEEEECCCEEEEEEEEEEECCCCCEEEEECCCCCCEEEECHHHHC
WQPHPMHPKALETSQHIARAVTADLGGQGLFGVELFVKGEQVWFSEVSPRPHDTGMVTMI
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEEE
TQWQNEFELHARAILGLPVSTALRAPGASAVIYGGVEAEGIVFDGVDEALRVPQTELRLF
EHHCHHHHHHHHHHCCCCHHHHHCCCCCCEEEECCCCCCCEEECCCHHHHHCCHHHHHEE
GKPESFTKRRMGVALAYAEDVDTARERAKEAAGKVKPRKA
CCCHHHHHHHHCEEEEEHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TTLGTPLSPSATKVMLLGSGELGKEVLIALQRLGVETIAVDRYENAPGQQVAHHARTIT
CCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHEEEE
MSDGEQLKALIEAERPDLVVPEIEAIATPMLEALEAAGVVRVIPTARAARLTMDREGIRR
ECCCHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHCCEEEEECCCHHHHEECCHHHHHH
LAAETLGLPTSPYKFCDSLEELQAAIDGGIGYPCVVKPVMSSSGKGQSKIDGPADVKAAW
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHH
DYAMAGGRVSHGRVIVEGFIDFDYEITLLTVRAVGANGEVETHFCEPIGHVQVAGDYVES
HHHHCCCCCCCCEEEEEEEECCCEEEEEEEEEEECCCCCEEEEECCCCCCEEEECHHHHC
WQPHPMHPKALETSQHIARAVTADLGGQGLFGVELFVKGEQVWFSEVSPRPHDTGMVTMI
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEEE
TQWQNEFELHARAILGLPVSTALRAPGASAVIYGGVEAEGIVFDGVDEALRVPQTELRLF
EHHCHHHHHHHHHHCCCCHHHHHCCCCCCEEEECCCCCCCEEECCCHHHHHCCHHHHHEE
GKPESFTKRRMGVALAYAEDVDTARERAKEAAGKVKPRKA
CCCHHHHHHHHCEEEEEHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA