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The map label for this gene is ywbI [H]

Identifier: 94310733

GI number: 94310733

Start: 1947324

End: 1948214

Strand: Reverse

Name: ywbI [H]

Synonym: Rmet_1795

Alternate gene names: 94310733

Gene position: 1948214-1947324 (Counterclockwise)

Preceding gene: 335055549

Following gene: 94310725

Centisome position: 49.6

GC content: 65.43

Gene sequence:

>891_bases
ATGCCGATCGACATTCGTGCGCTGCGTTACTTCGTGGAGACCGCGCGCCTGAGAAGCTTCACGCAGGCCGCATCTTCGCT
CTTCGTCACGCAATCGACCATCAGCAAGATGGTCAAGCAGCTCGAGGACGAAGTGGGGCAGCCACTGCTGATTCGCGAGG
GCAAGACCGTGCGCCTGACCGACGTCGGCAAGGTGGTCTATGACCGTGGCCAGGAAGCGCTCGGCGTGGTGCATCGGCTG
ACGCTCGAGGTGGCGGATCTTTCGTCACTTGGGCGCGGTCAATTGACGGTCGGTGTACCGCCAATGGTCAATCTGTTCTT
TTCCCCGGCCGTCAGCGCGTTTCGACGACGATATCCCAATCTGCAACTGAATCTCTCCGAGCACGGCGGGAAGATCGTGG
AGCAGCAGGTGGCGCATGGCGAACTCGAAGTCGGGGCCACGGTGCTGCCCGGAGACAGCGGGCTCAATCTGGAGACGCGC
CAGTTCGCCAAGTACCCGATCTGGGCGGTGGGCCCGCATCGCGCGGCCTGGGCAAGTGGCCGCACGGTCACGTTGGCGGC
GCTGCGCGACGAGCCACTGGTCATGCTGACCGACGAGTTCTCGCTGACCCGGATGCTGCGGCAGGCATTCACCGATGCTC
GCTTCGAGCCCCATGTGGTGGCGCAGAGCGGGCACTGGGACTTCCTGGCATCCATGGCTGCGGCGGGGCTCGGCACGACC
TTTCTGCCCGAACCGTTGCTCTCGCGCCTGGAGTCCCGTGACAAGCTTGCCGTCGCCCGGCTGACCGAGCCGACCGTGGA
CTGGGCGCTGGCCCATATCTGGTCCCCGGGGCGCTACCTGTCGCACGCCGCGCGGGCGTGGCTGGAGGTTTGCGAGGAGG
TGATGGGGTAA

Upstream 100 bases:

>100_bases
CTCGGTGGGGGCGTAGGAGCGGAAGTGTAACGGCCCTGCGAACATTCCAAACATGATTTAAAATTCCGGTCTGTAGTCGG
AATTTTCATGGGAACGGATC

Downstream 100 bases:

>100_bases
GGGGGAGAAAGCAGGCGGGAAGACAGCGGGAGAAGGCAGCGGGTCGGCTCAACGCGCCCGCAGCGCATCCACGATATTCA
GCCGCGACGCCCTGAGCGCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MPIDIRALRYFVETARLRSFTQAASSLFVTQSTISKMVKQLEDEVGQPLLIREGKTVRLTDVGKVVYDRGQEALGVVHRL
TLEVADLSSLGRGQLTVGVPPMVNLFFSPAVSAFRRRYPNLQLNLSEHGGKIVEQQVAHGELEVGATVLPGDSGLNLETR
QFAKYPIWAVGPHRAAWASGRTVTLAALRDEPLVMLTDEFSLTRMLRQAFTDARFEPHVVAQSGHWDFLASMAAAGLGTT
FLPEPLLSRLESRDKLAVARLTEPTVDWALAHIWSPGRYLSHAARAWLEVCEEVMG

Sequences:

>Translated_296_residues
MPIDIRALRYFVETARLRSFTQAASSLFVTQSTISKMVKQLEDEVGQPLLIREGKTVRLTDVGKVVYDRGQEALGVVHRL
TLEVADLSSLGRGQLTVGVPPMVNLFFSPAVSAFRRRYPNLQLNLSEHGGKIVEQQVAHGELEVGATVLPGDSGLNLETR
QFAKYPIWAVGPHRAAWASGRTVTLAALRDEPLVMLTDEFSLTRMLRQAFTDARFEPHVVAQSGHWDFLASMAAAGLGTT
FLPEPLLSRLESRDKLAVARLTEPTVDWALAHIWSPGRYLSHAARAWLEVCEEVMG
>Mature_295_residues
PIDIRALRYFVETARLRSFTQAASSLFVTQSTISKMVKQLEDEVGQPLLIREGKTVRLTDVGKVVYDRGQEALGVVHRLT
LEVADLSSLGRGQLTVGVPPMVNLFFSPAVSAFRRRYPNLQLNLSEHGGKIVEQQVAHGELEVGATVLPGDSGLNLETRQ
FAKYPIWAVGPHRAAWASGRTVTLAALRDEPLVMLTDEFSLTRMLRQAFTDARFEPHVVAQSGHWDFLASMAAAGLGTTF
LPEPLLSRLESRDKLAVARLTEPTVDWALAHIWSPGRYLSHAARAWLEVCEEVMG

Specific function: Positive Regulatory Protein For The Cyn Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=289, Percent_Identity=30.1038062283737, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1788297, Length=264, Percent_Identity=25.3787878787879, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1788748, Length=295, Percent_Identity=27.7966101694915, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790399, Length=295, Percent_Identity=27.1186440677966, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787879, Length=257, Percent_Identity=29.9610894941634, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788887, Length=244, Percent_Identity=27.4590163934426, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI2367136, Length=243, Percent_Identity=24.2798353909465, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790208, Length=253, Percent_Identity=23.7154150197628, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI145693105, Length=149, Percent_Identity=25.503355704698, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI145693193, Length=126, Percent_Identity=30.952380952381, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32741; Mature: 32610

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIDIRALRYFVETARLRSFTQAASSLFVTQSTISKMVKQLEDEVGQPLLIREGKTVRLT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEE
DVGKVVYDRGQEALGVVHRLTLEVADLSSLGRGQLTVGVPPMVNLFFSPAVSAFRRRYPN
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCCC
LQLNLSEHGGKIVEQQVAHGELEVGATVLPGDSGLNLETRQFAKYPIWAVGPHRAAWASG
EEEEEHHCCCHHHHHHHHCCCEEECCEEECCCCCCCCCHHHHHCCCCEEECCCCHHHCCC
RTVTLAALRDEPLVMLTDEFSLTRMLRQAFTDARFEPHVVAQSGHWDFLASMAAAGLGTT
CEEEEEEECCCCEEEEECCHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCCC
FLPEPLLSRLESRDKLAVARLTEPTVDWALAHIWSPGRYLSHAARAWLEVCEEVMG
CCCHHHHHHHHCCCCEEEHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
PIDIRALRYFVETARLRSFTQAASSLFVTQSTISKMVKQLEDEVGQPLLIREGKTVRLT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEE
DVGKVVYDRGQEALGVVHRLTLEVADLSSLGRGQLTVGVPPMVNLFFSPAVSAFRRRYPN
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCCC
LQLNLSEHGGKIVEQQVAHGELEVGATVLPGDSGLNLETRQFAKYPIWAVGPHRAAWASG
EEEEEHHCCCHHHHHHHHCCCEEECCEEECCCCCCCCCHHHHHCCCCEEECCCCHHHCCC
RTVTLAALRDEPLVMLTDEFSLTRMLRQAFTDARFEPHVVAQSGHWDFLASMAAAGLGTT
CEEEEEEECCCCEEEEECCHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCCC
FLPEPLLSRLESRDKLAVARLTEPTVDWALAHIWSPGRYLSHAARAWLEVCEEVMG
CCCHHHHHHHHCCCCEEEHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]