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The map label for this gene is alkK [H]

Identifier: 94310658

GI number: 94310658

Start: 1859568

End: 1861214

Strand: Direct

Name: alkK [H]

Synonym: Rmet_1718

Alternate gene names: 94310658

Gene position: 1859568-1861214 (Clockwise)

Preceding gene: 94310657

Following gene: 335055547

Centisome position: 47.34

GC content: 64.97

Gene sequence:

>1647_bases
ATGGAGAACCTGATCGTTTCCGCGCGTTCGGCCTACGCATACCCGTTGCTGATCAAGCAGCTCCTGATCAACGCCATGAG
CCTGTATGGCGACCAGGAGATCAGCTACCGCGGCGAGCTGCGCTACACCTATCGCGAATTCCGCGAGCGCGTGGGAAAGC
TGGCCAATTCGCTTTCGGCTCTCGGTGTGCATCACGGCTCCACAGTGGCCGTGATGGACTGGGACAGCCACCGCTACCTG
GAGTGCTATTTCGGCGTGCCGATGATGGGCGCTACGCTCTTCACGGTCAACGTTCGCCTGTCGCCGCAGCAGATCCTGTA
CACGCTGAATGACGCGGGCGCTGACGTCGTGCTGGTGCATCCGGACTTCGTGCCCGTGATCGACCAGATCCGGGACAAGC
TCACGTGCAAGCCACGCTTCGTGCTGATGGCAGACGGACAGACCGTGCCCGACACGGCTATCCCGTTCGCCGGCGAGTAC
GAGGCACTGGTCGGCGCCGCCTCGCCGGAGTTCGAGTTTCCGGAGTTTGACGAGCGCACGAAGGCCGCCACGTTCTATAC
GACAGGCACCACGGGCGACCCCAAGGGCGTGTGCTACAGCCATCGCGACATCGTGCTGCATACGCTGACCGCGACGGCCA
GCCTGTGCTCGCCGCGCGAAGGCCAGCGTATGCATCGCGAGGATGTGTATATGCCGATCACGCCGATGTTCCATGTGATG
GCGTGGGGCATCCCGTATGTTGCGATCACGCTGGGCCTGCGCACGGTCCTGCCGGGCCGCTACGCGCCCGAAATGCTGCT
GAAGCTGCGCGCCCAGGAAAAGGTGTCGTTCTCGCACTGCGTGCCGACAATCCTGCAGATGCTGCTGCACGCCGCAGAGG
CAGGCGGACAGGACCTGAGCGGCTGGAAGATCATCATCGGCGGTTCGGCGCTGCCACCGTCCTTGTGCGAGGCCGCGCTG
GAGCGGGGCATCGACATCTTTGCCGGCTACGGCATGTCCGAAACCGGGCCGATCGTGGCGCTGGCGCAGTTGCCGCCGGG
ATACCAGCCGGCCAGCCGCGAGGAAGAAGTCCGCCTGCGCTGCTCGACGGGCCGGCCGGTGCCGCTGGCAGACTTCCGCG
TCGTGGGGACGGACATGCGGGAACTGCCGCGCGACGGCCAAAGCCAGGGCGAGATCGTGCTGCGCGCGCCATACCTGACC
CGCAGTTACTTCCGCAAGCCGAGTGCGTCGGAAGAACTCTGGGCCGACGGGTACCTGCACACGCAGGACGTCGCGGTCGT
GCACGAGAACGGCTTCGTGCAGATCGTGGATCGCCTCAAGGACGTCATCAAGACCGGTGGCGAGTGGGTCTCGTCGATCG
AGATCGAGAACCTGGTCACGCGCGTGCCAGGCGTGCGGGAGTGCGCCGTGATTGGCGTGCCCGACGCGCGCTGGGGCGAG
CGGCCGATGGCCTTCGTGGTGCGGCAACCGGATTCGGAAGTGGGCGCCGAGGACATTCGCGCGGAGCTGATGAACCATGT
CTCGGCCCAGCGCCTGAGCAAGTTCGCGGTACCTGAAGCCGATCGGATCGCCTTTGTGGCCGAGATTCCGAAGACCAGCG
TTGGCAAAATTGACAAGAAGCTGCTACGGGGGAACAAAGCAGCCTGA

Upstream 100 bases:

>100_bases
CACAGCATTACGCCGTGATGGACTATCCGCCTTGCTATCGCAAGATGGAGCGCGCGGGCATTATCGGCAGCGTGCCGTTC
TGAGCAGTACCGGAGTCAAT

Downstream 100 bases:

>100_bases
GGGTAGGGCATGGAGTTAGGGAGCGCCCCGCACTGCGGGGCGTTTTTTTATTTGGCGACATGCAACCCGTGCATGTAGAC
GATGAAATCGGTGGATATGC

Product: AMP-dependent synthetase and ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MENLIVSARSAYAYPLLIKQLLINAMSLYGDQEISYRGELRYTYREFRERVGKLANSLSALGVHHGSTVAVMDWDSHRYL
ECYFGVPMMGATLFTVNVRLSPQQILYTLNDAGADVVLVHPDFVPVIDQIRDKLTCKPRFVLMADGQTVPDTAIPFAGEY
EALVGAASPEFEFPEFDERTKAATFYTTGTTGDPKGVCYSHRDIVLHTLTATASLCSPREGQRMHREDVYMPITPMFHVM
AWGIPYVAITLGLRTVLPGRYAPEMLLKLRAQEKVSFSHCVPTILQMLLHAAEAGGQDLSGWKIIIGGSALPPSLCEAAL
ERGIDIFAGYGMSETGPIVALAQLPPGYQPASREEEVRLRCSTGRPVPLADFRVVGTDMRELPRDGQSQGEIVLRAPYLT
RSYFRKPSASEELWADGYLHTQDVAVVHENGFVQIVDRLKDVIKTGGEWVSSIEIENLVTRVPGVRECAVIGVPDARWGE
RPMAFVVRQPDSEVGAEDIRAELMNHVSAQRLSKFAVPEADRIAFVAEIPKTSVGKIDKKLLRGNKAA

Sequences:

>Translated_548_residues
MENLIVSARSAYAYPLLIKQLLINAMSLYGDQEISYRGELRYTYREFRERVGKLANSLSALGVHHGSTVAVMDWDSHRYL
ECYFGVPMMGATLFTVNVRLSPQQILYTLNDAGADVVLVHPDFVPVIDQIRDKLTCKPRFVLMADGQTVPDTAIPFAGEY
EALVGAASPEFEFPEFDERTKAATFYTTGTTGDPKGVCYSHRDIVLHTLTATASLCSPREGQRMHREDVYMPITPMFHVM
AWGIPYVAITLGLRTVLPGRYAPEMLLKLRAQEKVSFSHCVPTILQMLLHAAEAGGQDLSGWKIIIGGSALPPSLCEAAL
ERGIDIFAGYGMSETGPIVALAQLPPGYQPASREEEVRLRCSTGRPVPLADFRVVGTDMRELPRDGQSQGEIVLRAPYLT
RSYFRKPSASEELWADGYLHTQDVAVVHENGFVQIVDRLKDVIKTGGEWVSSIEIENLVTRVPGVRECAVIGVPDARWGE
RPMAFVVRQPDSEVGAEDIRAELMNHVSAQRLSKFAVPEADRIAFVAEIPKTSVGKIDKKLLRGNKAA
>Mature_548_residues
MENLIVSARSAYAYPLLIKQLLINAMSLYGDQEISYRGELRYTYREFRERVGKLANSLSALGVHHGSTVAVMDWDSHRYL
ECYFGVPMMGATLFTVNVRLSPQQILYTLNDAGADVVLVHPDFVPVIDQIRDKLTCKPRFVLMADGQTVPDTAIPFAGEY
EALVGAASPEFEFPEFDERTKAATFYTTGTTGDPKGVCYSHRDIVLHTLTATASLCSPREGQRMHREDVYMPITPMFHVM
AWGIPYVAITLGLRTVLPGRYAPEMLLKLRAQEKVSFSHCVPTILQMLLHAAEAGGQDLSGWKIIIGGSALPPSLCEAAL
ERGIDIFAGYGMSETGPIVALAQLPPGYQPASREEEVRLRCSTGRPVPLADFRVVGTDMRELPRDGQSQGEIVLRAPYLT
RSYFRKPSASEELWADGYLHTQDVAVVHENGFVQIVDRLKDVIKTGGEWVSSIEIENLVTRVPGVRECAVIGVPDARWGE
RPMAFVVRQPDSEVGAEDIRAELMNHVSAQRLSKFAVPEADRIAFVAEIPKTSVGKIDKKLLRGNKAA

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=539, Percent_Identity=25.974025974026, Blast_Score=136, Evalue=6e-32,
Organism=Homo sapiens, GI187761345, Length=578, Percent_Identity=25.9515570934256, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI187761343, Length=578, Percent_Identity=25.9515570934256, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI42544132, Length=524, Percent_Identity=23.8549618320611, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI38505220, Length=522, Percent_Identity=25.2873563218391, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI157311624, Length=526, Percent_Identity=24.9049429657795, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI157311622, Length=526, Percent_Identity=24.9049429657795, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI58082049, Length=522, Percent_Identity=24.5210727969349, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI115511026, Length=531, Percent_Identity=23.5404896421846, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI28416953, Length=547, Percent_Identity=24.8628884826325, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI122937307, Length=532, Percent_Identity=24.0601503759398, Blast_Score=97, Evalue=6e-20,
Organism=Homo sapiens, GI13375727, Length=185, Percent_Identity=27.5675675675676, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1788107, Length=553, Percent_Identity=28.2097649186257, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI221142682, Length=510, Percent_Identity=25.6862745098039, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI145693145, Length=534, Percent_Identity=26.0299625468165, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1786810, Length=532, Percent_Identity=24.2481203007519, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788595, Length=446, Percent_Identity=23.9910313901345, Blast_Score=96, Evalue=8e-21,
Organism=Escherichia coli, GI1786801, Length=447, Percent_Identity=24.8322147651007, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1786529, Length=550, Percent_Identity=23.8181818181818, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17559526, Length=528, Percent_Identity=27.4621212121212, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI71996755, Length=538, Percent_Identity=23.9776951672862, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71994694, Length=586, Percent_Identity=23.8907849829352, Blast_Score=130, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI71994703, Length=586, Percent_Identity=23.8907849829352, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI71994690, Length=586, Percent_Identity=23.8907849829352, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17531443, Length=517, Percent_Identity=23.4042553191489, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17560308, Length=556, Percent_Identity=24.4604316546763, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17560140, Length=516, Percent_Identity=23.6434108527132, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17557194, Length=538, Percent_Identity=23.6059479553903, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17538037, Length=369, Percent_Identity=26.0162601626016, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI71985884, Length=367, Percent_Identity=26.158038147139, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17558820, Length=514, Percent_Identity=24.7081712062257, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI32563687, Length=511, Percent_Identity=24.0704500978474, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17556552, Length=354, Percent_Identity=25.1412429378531, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6319699, Length=375, Percent_Identity=24.8, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6319264, Length=536, Percent_Identity=25, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24581924, Length=576, Percent_Identity=26.9097222222222, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI18859661, Length=529, Percent_Identity=25.8979206049149, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI21355181, Length=540, Percent_Identity=23.8888888888889, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI19922652, Length=557, Percent_Identity=22.4416517055655, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI20130357, Length=372, Percent_Identity=25.8064516129032, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24653035, Length=371, Percent_Identity=24.7978436657682, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI21356947, Length=366, Percent_Identity=23.7704918032787, Blast_Score=96, Evalue=9e-20,
Organism=Drosophila melanogaster, GI161076582, Length=378, Percent_Identity=24.3386243386243, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24656500, Length=560, Percent_Identity=22.8571428571429, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648260, Length=367, Percent_Identity=24.7956403269755, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356441, Length=336, Percent_Identity=24.4047619047619, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24648257, Length=382, Percent_Identity=23.8219895287958, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281365686, Length=353, Percent_Identity=22.6628895184136, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI21358303, Length=326, Percent_Identity=23.9263803680982, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 60612; Mature: 60612

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENLIVSARSAYAYPLLIKQLLINAMSLYGDQEISYRGELRYTYREFRERVGKLANSLSA
CCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHH
LGVHHGSTVAVMDWDSHRYLECYFGVPMMGATLFTVNVRLSPQQILYTLNDAGADVVLVH
HEECCCCEEEEEECCCCCEEEEEECCCHHCCEEEEEEEEECHHHEEEEECCCCCCEEEEC
PDFVPVIDQIRDKLTCKPRFVLMADGQTVPDTAIPFAGEYEALVGAASPEFEFPEFDERT
CCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHHHEECCCCCCCCCCCCCCC
KAATFYTTGTTGDPKGVCYSHRDIVLHTLTATASLCSPREGQRMHREDVYMPITPMFHVM
CEEEEEECCCCCCCCCEEECCCCEEEEEHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHH
AWGIPYVAITLGLRTVLPGRYAPEMLLKLRAQEKVSFSHCVPTILQMLLHAAEAGGQDLS
HHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCC
GWKIIIGGSALPPSLCEAALERGIDIFAGYGMSETGPIVALAQLPPGYQPASREEEVRLR
CCEEEECCCCCCHHHHHHHHHCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCCEEEEE
CSTGRPVPLADFRVVGTDMRELPRDGQSQGEIVLRAPYLTRSYFRKPSASEELWADGYLH
ECCCCCCCCCCEEEECCCHHHCCCCCCCCCCEEEECCHHHHHHHCCCCCCCHHHCCCCEE
TQDVAVVHENGFVQIVDRLKDVIKTGGEWVSSIEIENLVTRVPGVRECAVIGVPDARWGE
ECCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
RPMAFVVRQPDSEVGAEDIRAELMNHVSAQRLSKFAVPEADRIAFVAEIPKTSVGKIDKK
CCEEEEEECCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHH
LLRGNKAA
HHCCCCCC
>Mature Secondary Structure
MENLIVSARSAYAYPLLIKQLLINAMSLYGDQEISYRGELRYTYREFRERVGKLANSLSA
CCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHH
LGVHHGSTVAVMDWDSHRYLECYFGVPMMGATLFTVNVRLSPQQILYTLNDAGADVVLVH
HEECCCCEEEEEECCCCCEEEEEECCCHHCCEEEEEEEEECHHHEEEEECCCCCCEEEEC
PDFVPVIDQIRDKLTCKPRFVLMADGQTVPDTAIPFAGEYEALVGAASPEFEFPEFDERT
CCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHHHEECCCCCCCCCCCCCCC
KAATFYTTGTTGDPKGVCYSHRDIVLHTLTATASLCSPREGQRMHREDVYMPITPMFHVM
CEEEEEECCCCCCCCCEEECCCCEEEEEHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHH
AWGIPYVAITLGLRTVLPGRYAPEMLLKLRAQEKVSFSHCVPTILQMLLHAAEAGGQDLS
HHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCC
GWKIIIGGSALPPSLCEAALERGIDIFAGYGMSETGPIVALAQLPPGYQPASREEEVRLR
CCEEEECCCCCCHHHHHHHHHCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCCEEEEE
CSTGRPVPLADFRVVGTDMRELPRDGQSQGEIVLRAPYLTRSYFRKPSASEELWADGYLH
ECCCCCCCCCCEEEECCCHHHCCCCCCCCCCEEEECCHHHHHHHCCCCCCCHHHCCCCEE
TQDVAVVHENGFVQIVDRLKDVIKTGGEWVSSIEIENLVTRVPGVRECAVIGVPDARWGE
ECCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
RPMAFVVRQPDSEVGAEDIRAELMNHVSAQRLSKFAVPEADRIAFVAEIPKTSVGKIDKK
CCEEEEEECCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHH
LLRGNKAA
HHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]