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The map label for this gene is oprM [H]

Identifier: 94310626

GI number: 94310626

Start: 1825603

End: 1827153

Strand: Reverse

Name: oprM [H]

Synonym: Rmet_1686

Alternate gene names: 94310626

Gene position: 1827153-1825603 (Counterclockwise)

Preceding gene: 94310627

Following gene: 94310625

Centisome position: 46.52

GC content: 63.51

Gene sequence:

>1551_bases
ATGGAATGGCTACCACGCATGTCACTTGCCTTTGCCAGAGAGTTACTCATCAAGCGTCAAATCTCACGTCGCGGACCTGC
CGCGGCACTGATAGTCGTCCCGATCCTGCTTGGCGGCTGCATCTCGATGGATCCCGCCTACGAGCGTCCTGCTGCACCGG
TGCGGACCACGTGGTCGCCAGATGCCGCGGCGCCGGCCATCACGCCAGCCGCCACAATAGGCTGGCGCGAGTTCTACGCG
GACCCCGAGCTTCAGGCACTGATCGGACAAGCGCTCGACAACAACCGGGACTTGCGCATCGGCGCCGCGCGTGTCGCGCA
ATCGCTTGCTGCCTACGGCATTCAGCGGGCGGAGCAATACCCGTGGATCGGTGCATCCGCCGATGGAACCCGAGCTCGGG
TACCCGGTGATCTGAATCTGACTGGTCGACCTCTAATAAGCAGCCAATATCAAGTTGGCGTTGGACTCAGCACGTGGGAG
ATCGATTTCTGGGGACGTATTCGCAGTCTCAAGGACGCCGCCTTGCAGAACTATCTGGCGTCCGATGCTGCGCAACGGGC
TGTCACACTGTCGATCATCACGCAGGTGGCGCAGACGTATCTGGCCATTCGCGAGCTCGACGAACGGATCGCTCTGGCAA
AGCAGACCACGGACACCCGGGCCGAATCGCTGCGTATCTTCACGCGCCGGGAACAAGTCGGTTCCGCATCCAAGCTGGAG
CTGACCGAAGTCATCACCCTTTACCAGCAGGCCCGGGCACTGGTCACCCAACTCGAACAGGACCGCGCCACACAGTCTCA
TGCGCTGGAGGTTCTGGTGGGCGCGCCGGTCGACGTCAAGCCGGCCATCGGCCTGGTCAACGATGCCGAACTGATGCGCC
AGATCGACGCTGGCCTGCCATCCGAGCAACTGGAGAACCGCCCCGACATCGTCTCGGCCGAGCACCAGTTGATGGCTGAA
AACGCAAACATCGGGGCTGCGCGCGCGGCGTTCTTCCCGCGAGTGACCCTGACCGGTTTCGCCGGCACGGCCAGTGCGGA
CCTGGATGGTCTGTTCCGTGGAGGCAGTGGCGTATGGTCCTTCGCCCCAAGCATTTCCATTCCGATCTTCCAGGGCGGAC
AGTTGCGTAGCAACCTCGACCTCGCGAAAGCGCGGCGCGTGGAGGCGGTAGCCCAATACGAGAAAACCATCCAGAATGCT
TTCCGCGATGTGGCCGATGCCCTGAGCGCGCAGCGCTGGCTGGCCGAACAGGTCGATATTCTGCAACTCACACAGGCGGC
ACAGGCCGAACGCCGGCGACTGGCGAAACTGCGGTACGACAATGGTGCAGCAGCCTTCCTCGAAGTCCTCGATGCCGAGC
GCGATCTGCTTGCCATCGAGCAACAGGCCGTCCAATCGCGCCGCGCCCTGCTTGCCAGCCGCGTCAGCCTGTATGCCGCA
CTGGGCGGTGGCACAGCCGTTGCCTCGCCGCCGGAGGCGGCCGCTCCGGAAGGTGCGCCCGCCAGTGCAGCGCCTGACGC
CAAGCCCTCCACACCTTCTACTGCTCGTTAA

Upstream 100 bases:

>100_bases
ACGCAGAGCTGCGATATTCTGGGGGAAACCCTGACGGGGCACTCCGCGTGCCCCATGCCCCCGGATTTTTTCACGGCGCG
CCATGCGCGGTGATGCCTGC

Downstream 100 bases:

>100_bases
CCAAGTCGGCCCATGAAGCTTCCTACCAACAAGCTCGTTCCCGCCCTGGTCGTGCTCGCTGCCGTCGCCGGCGGCTGGTA
CGCATGGGATCGCCTGCGCG

Product: RND efflux system, outer membrane lipoprotein, NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MEWLPRMSLAFARELLIKRQISRRGPAAALIVVPILLGGCISMDPAYERPAAPVRTTWSPDAAAPAITPAATIGWREFYA
DPELQALIGQALDNNRDLRIGAARVAQSLAAYGIQRAEQYPWIGASADGTRARVPGDLNLTGRPLISSQYQVGVGLSTWE
IDFWGRIRSLKDAALQNYLASDAAQRAVTLSIITQVAQTYLAIRELDERIALAKQTTDTRAESLRIFTRREQVGSASKLE
LTEVITLYQQARALVTQLEQDRATQSHALEVLVGAPVDVKPAIGLVNDAELMRQIDAGLPSEQLENRPDIVSAEHQLMAE
NANIGAARAAFFPRVTLTGFAGTASADLDGLFRGGSGVWSFAPSISIPIFQGGQLRSNLDLAKARRVEAVAQYEKTIQNA
FRDVADALSAQRWLAEQVDILQLTQAAQAERRRLAKLRYDNGAAAFLEVLDAERDLLAIEQQAVQSRRALLASRVSLYAA
LGGGTAVASPPEAAAPEGAPASAAPDAKPSTPSTAR

Sequences:

>Translated_516_residues
MEWLPRMSLAFARELLIKRQISRRGPAAALIVVPILLGGCISMDPAYERPAAPVRTTWSPDAAAPAITPAATIGWREFYA
DPELQALIGQALDNNRDLRIGAARVAQSLAAYGIQRAEQYPWIGASADGTRARVPGDLNLTGRPLISSQYQVGVGLSTWE
IDFWGRIRSLKDAALQNYLASDAAQRAVTLSIITQVAQTYLAIRELDERIALAKQTTDTRAESLRIFTRREQVGSASKLE
LTEVITLYQQARALVTQLEQDRATQSHALEVLVGAPVDVKPAIGLVNDAELMRQIDAGLPSEQLENRPDIVSAEHQLMAE
NANIGAARAAFFPRVTLTGFAGTASADLDGLFRGGSGVWSFAPSISIPIFQGGQLRSNLDLAKARRVEAVAQYEKTIQNA
FRDVADALSAQRWLAEQVDILQLTQAAQAERRRLAKLRYDNGAAAFLEVLDAERDLLAIEQQAVQSRRALLASRVSLYAA
LGGGTAVASPPEAAAPEGAPASAAPDAKPSTPSTAR
>Mature_516_residues
MEWLPRMSLAFARELLIKRQISRRGPAAALIVVPILLGGCISMDPAYERPAAPVRTTWSPDAAAPAITPAATIGWREFYA
DPELQALIGQALDNNRDLRIGAARVAQSLAAYGIQRAEQYPWIGASADGTRARVPGDLNLTGRPLISSQYQVGVGLSTWE
IDFWGRIRSLKDAALQNYLASDAAQRAVTLSIITQVAQTYLAIRELDERIALAKQTTDTRAESLRIFTRREQVGSASKLE
LTEVITLYQQARALVTQLEQDRATQSHALEVLVGAPVDVKPAIGLVNDAELMRQIDAGLPSEQLENRPDIVSAEHQLMAE
NANIGAARAAFFPRVTLTGFAGTASADLDGLFRGGSGVWSFAPSISIPIFQGGQLRSNLDLAKARRVEAVAQYEKTIQNA
FRDVADALSAQRWLAEQVDILQLTQAAQAERRRLAKLRYDNGAAAFLEVLDAERDLLAIEQQAVQSRRALLASRVSLYAA
LGGGTAVASPPEAAAPEGAPASAAPDAKPSTPSTAR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=456, Percent_Identity=36.8421052631579, Blast_Score=295, Evalue=5e-81,
Organism=Escherichia coli, GI2367348, Length=494, Percent_Identity=26.5182186234818, Blast_Score=105, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 55587; Mature: 55587

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEWLPRMSLAFARELLIKRQISRRGPAAALIVVPILLGGCISMDPAYERPAAPVRTTWSP
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCC
DAAAPAITPAATIGWREFYADPELQALIGQALDNNRDLRIGAARVAQSLAAYGIQRAEQY
CCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCC
PWIGASADGTRARVPGDLNLTGRPLISSQYQVGVGLSTWEIDFWGRIRSLKDAALQNYLA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHHHHHH
SDAAQRAVTLSIITQVAQTYLAIRELDERIALAKQTTDTRAESLRIFTRREQVGSASKLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHH
LTEVITLYQQARALVTQLEQDRATQSHALEVLVGAPVDVKPAIGLVNDAELMRQIDAGLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCC
SEQLENRPDIVSAEHQLMAENANIGAARAAFFPRVTLTGFAGTASADLDGLFRGGSGVWS
HHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHCCCEEEECCCCCCCCCHHHHHCCCCCCEE
FAPSISIPIFQGGQLRSNLDLAKARRVEAVAQYEKTIQNAFRDVADALSAQRWLAEQVDI
CCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQLTQAAQAERRRLAKLRYDNGAAAFLEVLDAERDLLAIEQQAVQSRRALLASRVSLYAA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGGGTAVASPPEAAAPEGAPASAAPDAKPSTPSTAR
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEWLPRMSLAFARELLIKRQISRRGPAAALIVVPILLGGCISMDPAYERPAAPVRTTWSP
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCC
DAAAPAITPAATIGWREFYADPELQALIGQALDNNRDLRIGAARVAQSLAAYGIQRAEQY
CCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCC
PWIGASADGTRARVPGDLNLTGRPLISSQYQVGVGLSTWEIDFWGRIRSLKDAALQNYLA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHHHHHH
SDAAQRAVTLSIITQVAQTYLAIRELDERIALAKQTTDTRAESLRIFTRREQVGSASKLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHH
LTEVITLYQQARALVTQLEQDRATQSHALEVLVGAPVDVKPAIGLVNDAELMRQIDAGLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCC
SEQLENRPDIVSAEHQLMAENANIGAARAAFFPRVTLTGFAGTASADLDGLFRGGSGVWS
HHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHCCCEEEECCCCCCCCCHHHHHCCCCCCEE
FAPSISIPIFQGGQLRSNLDLAKARRVEAVAQYEKTIQNAFRDVADALSAQRWLAEQVDI
CCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQLTQAAQAERRRLAKLRYDNGAAAFLEVLDAERDLLAIEQQAVQSRRALLASRVSLYAA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGGGTAVASPPEAAAPEGAPASAAPDAKPSTPSTAR
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]