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The map label for this gene is phaC2 [H]

Identifier: 94310611

GI number: 94310611

Start: 1807369

End: 1809087

Strand: Reverse

Name: phaC2 [H]

Synonym: Rmet_1671

Alternate gene names: 94310611

Gene position: 1809087-1807369 (Counterclockwise)

Preceding gene: 94310612

Following gene: 94310607

Centisome position: 46.06

GC content: 61.78

Gene sequence:

>1719_bases
ATGAGCCGCGCATCCGCCCGAAACAACACGCCATTCGCCATGAGAACTCCAATGCCTGTGCCGACACCGCATGCCAATCC
GCTGCTGAGCGGTTTTCCCTCGCCGCTGACGACGCTCGCGGATCTATTCGCCTACCCGCGCTACCTGCCCAGCGGCATCG
ACATGCCCCGCCTGATGCAGTCCGGCTTCCGGCACCTTGAAGGTCAGTTTTCGATACTGGCCTCGGCATTCGGCCAGGCC
GGTGCCGACCAAAAACCGGACCGCCGGTTCGCGGTGAAGGACTGGGACGAGAACCCGTGGTACCGGATGCTCAAGCAAGG
TTATCTGCTCAATGCCAAGACGCTTGCCGAACTGGCCGAGTCGATCCATCTGGACGACGACGACAAGAGTAAGCTGACCT
TTTTCCTGGGGCAACTCGTCGATGCCATGAGCCCGTCCAACTTCCTGGCAACCAACCCGGAAGCAATCCGGCTCGCACTG
GAGACCAACGGTGAAAGCCTCTTGAACGGCTTCGTGAACTATGTCGAGGACATGCGCCAGCGGCGCATCGCCATCACCGA
CGAGTCGGCGTTCGAGGTGGGCCGTGACGTCGCCACATCCGAAGGCGCTGTGGTGTTCGAATGCGATGTGATGCAGCTGA
TCCAGTACAAGCCGCTGACATCGAAGGTGATGTCGCGCCCGCTGGTGATCGTGCCGCCTTGCATCAACAAGTACTACATC
CTCGATCTCCAGCCGGAGAACTCTTTCGTCCGCTTTGCCTGCGAACAGGGTTACACGGTCTTTGTCACTTCGTGGCGCAA
TCCGCAGGAAGACATGCGCGATACCACCTGGGACGACTATGTGGAAGGTGTCATCAAGGCGCTCGAGGTCAGCCGTGCCA
TCGGTGGCGCAAGCCAGGTGAACGCGCTGGGCTGGTGCGTCGGCGGCACGCTGCTGACCTCGGCGCTGGCCGTCCAGGAG
GCACGCGGTGAAGACATGGTGGCCAGCATGACCTTGCTGACGACCATGCTCGACTTTACCGACCCGGGTGACCTCGGTTT
CTTCCTCGACGAAGCCACTGTCGTGGCGCGCGAGCAGACCATTGGCAACGGTGGCATTTACTCCGGCAAGGAACTGGCCT
TCGTCTTCCAGACCCTGCGACCCAACGACCTGATCTGGCCGTACGTCGTCACCAACTACCTGAAGGGAAAATCGCCGGCG
GCGTTCGACCTGCTCTATTGGAACGCCGATGCGACCAACCTGCCCGGCCCCATGTACGCGTGGTACCTGCGCAATATGTA
CCTGGAAAACCGGCTATGCAAACGCGACGAGTTGTCGATGTGCGGGCATTCGATCGACCTGGGCAAGATCAAGGTGCCGA
GCTACATCCTGGCGACCAAGGAAGATCACATCGTCCCCTGGCAGTCAGCCTATCGCAGCACGCAACTGCTCGGTGGCGAT
AGCGAGTTCGTGCTGGGCGAAAGTGGCCACATCGCCGGTGTCATCAATCCGGCCTCCAAGCAGAAGCGCGGCTACTGGAG
CGCCGGCGCGCAGGGAGATCATGCCGAGGACTGGCTCGCTTCCGCACAGTCCACGCCCGGAAGCTGGTGGACGCACTGGA
GCGCCTGGCTCAAGCGTCACGGCGGCAAGCAAAAGGCGGCGCCGGCGAAGCTCGGCGACAGCCGATTCCAGCCTATCGAA
CCCGCGCCCGGCCGGTACGTGAAGGTCAGGGTCGAGTAA

Upstream 100 bases:

>100_bases
AACGGCGTGTCGCGCTCAGCACGGTCTGCATGGTCCAGGACAAGGTCGTGGTCGACGGTGACGCCCTGATCATGCCGACG
TCAGCAAAACGACGCGAGCA

Downstream 100 bases:

>100_bases
GTCTCAGCCGCCATTGCGTGCAACACTCGCGGCTACGAGCACGATGGCCGCGAGCCCGGCTGTTGCCGTGACCTCCATGC
GGTGATTGGGGCGAAGGAAC

Product: Poly(3-hydroxybutyrate) polymerase

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]

Number of amino acids: Translated: 572; Mature: 571

Protein sequence:

>572_residues
MSRASARNNTPFAMRTPMPVPTPHANPLLSGFPSPLTTLADLFAYPRYLPSGIDMPRLMQSGFRHLEGQFSILASAFGQA
GADQKPDRRFAVKDWDENPWYRMLKQGYLLNAKTLAELAESIHLDDDDKSKLTFFLGQLVDAMSPSNFLATNPEAIRLAL
ETNGESLLNGFVNYVEDMRQRRIAITDESAFEVGRDVATSEGAVVFECDVMQLIQYKPLTSKVMSRPLVIVPPCINKYYI
LDLQPENSFVRFACEQGYTVFVTSWRNPQEDMRDTTWDDYVEGVIKALEVSRAIGGASQVNALGWCVGGTLLTSALAVQE
ARGEDMVASMTLLTTMLDFTDPGDLGFFLDEATVVAREQTIGNGGIYSGKELAFVFQTLRPNDLIWPYVVTNYLKGKSPA
AFDLLYWNADATNLPGPMYAWYLRNMYLENRLCKRDELSMCGHSIDLGKIKVPSYILATKEDHIVPWQSAYRSTQLLGGD
SEFVLGESGHIAGVINPASKQKRGYWSAGAQGDHAEDWLASAQSTPGSWWTHWSAWLKRHGGKQKAAPAKLGDSRFQPIE
PAPGRYVKVRVE

Sequences:

>Translated_572_residues
MSRASARNNTPFAMRTPMPVPTPHANPLLSGFPSPLTTLADLFAYPRYLPSGIDMPRLMQSGFRHLEGQFSILASAFGQA
GADQKPDRRFAVKDWDENPWYRMLKQGYLLNAKTLAELAESIHLDDDDKSKLTFFLGQLVDAMSPSNFLATNPEAIRLAL
ETNGESLLNGFVNYVEDMRQRRIAITDESAFEVGRDVATSEGAVVFECDVMQLIQYKPLTSKVMSRPLVIVPPCINKYYI
LDLQPENSFVRFACEQGYTVFVTSWRNPQEDMRDTTWDDYVEGVIKALEVSRAIGGASQVNALGWCVGGTLLTSALAVQE
ARGEDMVASMTLLTTMLDFTDPGDLGFFLDEATVVAREQTIGNGGIYSGKELAFVFQTLRPNDLIWPYVVTNYLKGKSPA
AFDLLYWNADATNLPGPMYAWYLRNMYLENRLCKRDELSMCGHSIDLGKIKVPSYILATKEDHIVPWQSAYRSTQLLGGD
SEFVLGESGHIAGVINPASKQKRGYWSAGAQGDHAEDWLASAQSTPGSWWTHWSAWLKRHGGKQKAAPAKLGDSRFQPIE
PAPGRYVKVRVE
>Mature_571_residues
SRASARNNTPFAMRTPMPVPTPHANPLLSGFPSPLTTLADLFAYPRYLPSGIDMPRLMQSGFRHLEGQFSILASAFGQAG
ADQKPDRRFAVKDWDENPWYRMLKQGYLLNAKTLAELAESIHLDDDDKSKLTFFLGQLVDAMSPSNFLATNPEAIRLALE
TNGESLLNGFVNYVEDMRQRRIAITDESAFEVGRDVATSEGAVVFECDVMQLIQYKPLTSKVMSRPLVIVPPCINKYYIL
DLQPENSFVRFACEQGYTVFVTSWRNPQEDMRDTTWDDYVEGVIKALEVSRAIGGASQVNALGWCVGGTLLTSALAVQEA
RGEDMVASMTLLTTMLDFTDPGDLGFFLDEATVVAREQTIGNGGIYSGKELAFVFQTLRPNDLIWPYVVTNYLKGKSPAA
FDLLYWNADATNLPGPMYAWYLRNMYLENRLCKRDELSMCGHSIDLGKIKVPSYILATKEDHIVPWQSAYRSTQLLGGDS
EFVLGESGHIAGVINPASKQKRGYWSAGAQGDHAEDWLASAQSTPGSWWTHWSAWLKRHGGKQKAAPAKLGDSRFQPIEP
APGRYVKVRVE

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010963
- InterPro:   IPR010941 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N [H]

EC number: NA

Molecular weight: Translated: 63690; Mature: 63559

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRASARNNTPFAMRTPMPVPTPHANPLLSGFPSPLTTLADLFAYPRYLPSGIDMPRLMQ
CCCCCCCCCCCEEEECCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
SGFRHLEGQFSILASAFGQAGADQKPDRRFAVKDWDENPWYRMLKQGYLLNAKTLAELAE
HHHHHHCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHCCCEECHHHHHHHHH
SIHLDDDDKSKLTFFLGQLVDAMSPSNFLATNPEAIRLALETNGESLLNGFVNYVEDMRQ
HCCCCCCCHHHHHHHHHHHHHHCCCCCCEECCHHHEEEEEECCCHHHHHHHHHHHHHHHH
RRIAITDESAFEVGRDVATSEGAVVFECDVMQLIQYKPLTSKVMSRPLVIVPPCINKYYI
CEEEECCCHHHHHCCHHCCCCCCEEEECHHHHHHHCCCHHHHHHHCCEEEECCCCCCEEE
LDLQPENSFVRFACEQGYTVFVTSWRNPQEDMRDTTWDDYVEGVIKALEVSRAIGGASQV
EEECCCCCEEEEEECCCCEEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHH
NALGWCVGGTLLTSALAVQEARGEDMVASMTLLTTMLDFTDPGDLGFFLDEATVVAREQT
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEHHHC
IGNGGIYSGKELAFVFQTLRPNDLIWPYVVTNYLKGKSPAAFDLLYWNADATNLPGPMYA
CCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHH
WYLRNMYLENRLCKRDELSMCGHSIDLGKIKVPSYILATKEDHIVPWQSAYRSTQLLGGD
HHHHHHHHHHHHCCCHHHHHCCCCCCCCCEECCCEEEECCCCCCCCHHHHHHHHHEECCC
SEFVLGESGHIAGVINPASKQKRGYWSAGAQGDHAEDWLASAQSTPGSWWTHWSAWLKRH
CCEEECCCCCEEEEECCCHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHC
GGKQKAAPAKLGDSRFQPIEPAPGRYVKVRVE
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure 
SRASARNNTPFAMRTPMPVPTPHANPLLSGFPSPLTTLADLFAYPRYLPSGIDMPRLMQ
CCCCCCCCCCEEEECCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
SGFRHLEGQFSILASAFGQAGADQKPDRRFAVKDWDENPWYRMLKQGYLLNAKTLAELAE
HHHHHHCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHCCCEECHHHHHHHHH
SIHLDDDDKSKLTFFLGQLVDAMSPSNFLATNPEAIRLALETNGESLLNGFVNYVEDMRQ
HCCCCCCCHHHHHHHHHHHHHHCCCCCCEECCHHHEEEEEECCCHHHHHHHHHHHHHHHH
RRIAITDESAFEVGRDVATSEGAVVFECDVMQLIQYKPLTSKVMSRPLVIVPPCINKYYI
CEEEECCCHHHHHCCHHCCCCCCEEEECHHHHHHHCCCHHHHHHHCCEEEECCCCCCEEE
LDLQPENSFVRFACEQGYTVFVTSWRNPQEDMRDTTWDDYVEGVIKALEVSRAIGGASQV
EEECCCCCEEEEEECCCCEEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHH
NALGWCVGGTLLTSALAVQEARGEDMVASMTLLTTMLDFTDPGDLGFFLDEATVVAREQT
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEHHHC
IGNGGIYSGKELAFVFQTLRPNDLIWPYVVTNYLKGKSPAAFDLLYWNADATNLPGPMYA
CCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHH
WYLRNMYLENRLCKRDELSMCGHSIDLGKIKVPSYILATKEDHIVPWQSAYRSTQLLGGD
HHHHHHHHHHHHCCCHHHHHCCCCCCCCCEECCCEEEECCCCCCCCHHHHHHHHHEECCC
SEFVLGESGHIAGVINPASKQKRGYWSAGAQGDHAEDWLASAQSTPGSWWTHWSAWLKRH
CCEEECCCCCEEEEECCCHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHC
GGKQKAAPAKLGDSRFQPIEPAPGRYVKVRVE
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2670936; 1987116 [H]