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The map label for this gene is bbuR [H]

Identifier: 94310573

GI number: 94310573

Start: 1759872

End: 1760807

Strand: Reverse

Name: bbuR [H]

Synonym: Rmet_1631

Alternate gene names: 94310573

Gene position: 1760807-1759872 (Counterclockwise)

Preceding gene: 94310574

Following gene: 94310571

Centisome position: 44.83

GC content: 67.95

Gene sequence:

>936_bases
ATGTCGGCACTTAACTCCGACGATCTCGCGTGCTTTATCTGCGTGGCCAACACGCAGAGCATTTCGCGCGCGGCGCTGGA
ACTGGGCGCGGACCAGTCGACCGTAAGCCGGCAGATCGCACGGCTCGAGGCGTCGCTGGACACGCGCCTGTTTCACCGCA
GCGGCCGCGGCGTGGTGCTGACCGAAGCGGGCAATACGCTGCTAGGCTACGCACGGCAGGTGGCCGCCACGCTGACCGAG
GCGCGCGCCGCCGTGCGCGCCTCGACCGCCCAGGGCCCGGCACAGCTCGTGATCGCGGCTCAGCCAACCATCGCCAACAC
CGCCTTCGCGGCCATTGGCACCGCGCTGAAGACACGCTTTCCGGCAACAAAGCTGCGTTTCGTGGAAGGGCTGGCCAGCC
CTATTCTGGCGTGGCTAGCCGCCGGAGAGATCGACGTCGCCCTGCTCTACCTGCCTGAGCAGCAAGGCGCGCTCAAGGTC
GACATCCTGCTCGAGGAAGACCTGACACTGGTCACGCCCACGTCGTGGAGCCATCTCGGACCGACGTTCCAGGCCCGCCG
CCTGGGTGAAGTGCCGATGATCCTGCCCAGCACCGGGCACGGCCTGCGCGTGCTGGCCGAATCGCTGGCCGCACGGGTCG
GCACCACGCTGAACATGGCGCTGGAATGCGATGCGTCGAACACGGTGTCGATGCGGCTCGTCGAAGACGGTTGTGGCGCG
ACGCTGTTGCCATTCGCCGCAGTGGCGGACCGCGTCGCACAAGGCCGCCTGCATTCCGCGCGGCTGGTCGATCCGGTGGT
CACCCGCCAGGTAGCGCTGGCCACGGCACGGAACCGGCCACCTGTGCCGGAACTCTGGGACATCATGCAGACGGTGCGGC
TTTCGGTCCGGAATATCGTGATGTCGGGGGCCTGGCCGGGGGCGCGGCTCATCTAA

Upstream 100 bases:

>100_bases
GCGGCGCGGGCTGGGATCACTCGGGACTGGTCAAAGCGATCGAGCACATGTCGTCGTTCGCGATCGGTGACAAGCCGACC
AACGCTCCGACCAAAGGCTG

Downstream 100 bases:

>100_bases
GCAAGATCCGAGCCCCCTCGGGCCATCATCGTGCGGATATCCCGCGTTCATGCAGTGTCAATGCAGATCGGCATCCGGTG
CGTCAGCGTGGCGGTTGGCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MSALNSDDLACFICVANTQSISRAALELGADQSTVSRQIARLEASLDTRLFHRSGRGVVLTEAGNTLLGYARQVAATLTE
ARAAVRASTAQGPAQLVIAAQPTIANTAFAAIGTALKTRFPATKLRFVEGLASPILAWLAAGEIDVALLYLPEQQGALKV
DILLEEDLTLVTPTSWSHLGPTFQARRLGEVPMILPSTGHGLRVLAESLAARVGTTLNMALECDASNTVSMRLVEDGCGA
TLLPFAAVADRVAQGRLHSARLVDPVVTRQVALATARNRPPVPELWDIMQTVRLSVRNIVMSGAWPGARLI

Sequences:

>Translated_311_residues
MSALNSDDLACFICVANTQSISRAALELGADQSTVSRQIARLEASLDTRLFHRSGRGVVLTEAGNTLLGYARQVAATLTE
ARAAVRASTAQGPAQLVIAAQPTIANTAFAAIGTALKTRFPATKLRFVEGLASPILAWLAAGEIDVALLYLPEQQGALKV
DILLEEDLTLVTPTSWSHLGPTFQARRLGEVPMILPSTGHGLRVLAESLAARVGTTLNMALECDASNTVSMRLVEDGCGA
TLLPFAAVADRVAQGRLHSARLVDPVVTRQVALATARNRPPVPELWDIMQTVRLSVRNIVMSGAWPGARLI
>Mature_310_residues
SALNSDDLACFICVANTQSISRAALELGADQSTVSRQIARLEASLDTRLFHRSGRGVVLTEAGNTLLGYARQVAATLTEA
RAAVRASTAQGPAQLVIAAQPTIANTAFAAIGTALKTRFPATKLRFVEGLASPILAWLAAGEIDVALLYLPEQQGALKVD
ILLEEDLTLVTPTSWSHLGPTFQARRLGEVPMILPSTGHGLRVLAESLAARVGTTLNMALECDASNTVSMRLVEDGCGAT
LLPFAAVADRVAQGRLHSARLVDPVVTRQVALATARNRPPVPELWDIMQTVRLSVRNIVMSGAWPGARLI

Specific function: Positive Regulatory Protein For The Cyn Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=276, Percent_Identity=27.536231884058, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1790399, Length=249, Percent_Identity=28.5140562248996, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788748, Length=289, Percent_Identity=25.9515570934256, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790208, Length=243, Percent_Identity=27.5720164609054, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787601, Length=162, Percent_Identity=27.1604938271605, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788297, Length=245, Percent_Identity=24.8979591836735, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI145693105, Length=269, Percent_Identity=24.5353159851301, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788481, Length=250, Percent_Identity=25.2, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32989; Mature: 32857

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALNSDDLACFICVANTQSISRAALELGADQSTVSRQIARLEASLDTRLFHRSGRGVVL
CCCCCCCCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
TEAGNTLLGYARQVAATLTEARAAVRASTAQGPAQLVIAAQPTIANTAFAAIGTALKTRF
ECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCC
PATKLRFVEGLASPILAWLAAGEIDVALLYLPEQQGALKVDILLEEDLTLVTPTSWSHLG
CHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCEEEEEEEECCCEEECCCCCCCCC
PTFQARRLGEVPMILPSTGHGLRVLAESLAARVGTTLNMALECDASNTVSMRLVEDGCGA
CCHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHCCEEEEEEEECCCCCEEEEEEECCCCC
TLLPFAAVADRVAQGRLHSARLVDPVVTRQVALATARNRPPVPELWDIMQTVRLSVRNIV
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
MSGAWPGARLI
HCCCCCCCCCC
>Mature Secondary Structure 
SALNSDDLACFICVANTQSISRAALELGADQSTVSRQIARLEASLDTRLFHRSGRGVVL
CCCCCCCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
TEAGNTLLGYARQVAATLTEARAAVRASTAQGPAQLVIAAQPTIANTAFAAIGTALKTRF
ECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCC
PATKLRFVEGLASPILAWLAAGEIDVALLYLPEQQGALKVDILLEEDLTLVTPTSWSHLG
CHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCEEEEEEEECCCEEECCCCCCCCC
PTFQARRLGEVPMILPSTGHGLRVLAESLAARVGTTLNMALECDASNTVSMRLVEDGCGA
CCHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHCCEEEEEEEECCCCCEEEEEEECCCCC
TLLPFAAVADRVAQGRLHSARLVDPVVTRQVALATARNRPPVPELWDIMQTVRLSVRNIV
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
MSGAWPGARLI
HCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9524276; 12910271 [H]