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The map label for this gene is puuE [H]

Identifier: 94310560

GI number: 94310560

Start: 1745040

End: 1746302

Strand: Direct

Name: puuE [H]

Synonym: Rmet_1618

Alternate gene names: 94310560

Gene position: 1745040-1746302 (Clockwise)

Preceding gene: 94310559

Following gene: 94310561

Centisome position: 44.42

GC content: 67.54

Gene sequence:

>1263_bases
ATGAAGAACGCCGAACTGGTCCGTCGCAAGGATGCCGCTACCCCGCGTGGGGTAGGTGTGATGTGCAATTTCTACGCCGA
CCGCGCCGAGAACAGCGAAATCTGGGATGTCGAGGGCAAGCGGTACATCGACTTCGCTGCCGGCATCGCCGTGCTCAACA
CGGGCCATCGTCACCCGCGCCTGGTGGCCGCGCTGCAGGCGCAACTGAATCGCTTCACCCATACGGCGTACCAGATCGTG
CCGTACGCCAGCTATGTGGAGCTGGCCGAGAAGATCAACCAGCGCGCGCCGGGCCGCTCAGCTCGCAAGACGGCCTTCTT
CACGACCGGCGCGGAAGCGGTGGAGAACGCCGTCAAGATCGCGCGCGCCGCCACCGGCCGGCCCGGCGTGATCGCGTTCT
CAGGCGGGTTCCATGGCCGCACGATGATGGGCATGGCGCTGACTGGCAAGGTGGCCCCGTACAAGACCGGCTTCGGGCCG
TTCCCCGGCGAGGTCTTCCACGCGCCTTACCCGTCTGCATTGCATGGCGTGACGGTGGAGGATTCGCGCCGCGCGCTCGA
ACACCTGTTCAAGGCCGATATCGACCCGCGCCGCGTGGCAGCCATCATCTTCGAACCGGTGCAGGGCGAAGGCGGCTTCA
ACGTGGCGCCGCCGGAGTTCGTGCGGATGCTGCGCGCTGTGTGCGACGAGCACGGCATCCTGCTGGTGGCCGATGAGGTC
CAGACCGGTTTCGGCCGCACGGGCAAGCTGTTCGCGATGGAGCACTACGACGTCGCGCCGGACCTGACCACGATGGCCAA
GAGCCTGGCCGGCGGGATGCCGCTTTCCGCCGTGTGTGGCCGTGCCGAAATCATGGATGCGCCCGCGCCGGGCGGCCTCG
GCGGGACCTACGCCGGCAATCCGCTGGCGGTGGCGTCCGCGCTGGCCGTGCTCGACGTGCTCGAGTCCGAGAACCTGATC
GCGCGAGGCGCGGAGCTTGGCGAACGACTGACGGCCCGCCTGAACAGCCTCAAGCCGCGCGTGCCGCAAATCGCCGAAGT
CCGTGGCATCGGCGCAATGGTGGCCGTGGAGTTCCGTCGCGCCGATGGCAGCCCGGATGCGGAGTTCACCCGCGAAGTGC
AGAACCGCGCGCTGGAGAGGGGCCTGTTGCTGCTGTCGTGCGGCGTGTACGGCAACGTGATTCGCTTCCTGTTCCCGCTG
ACCATCCAGGACGCCGTGATGAACGAGGGCCTCGACATCCTGGCAGACGTGCTGACTCGCTGA

Upstream 100 bases:

>100_bases
GATCGACGCCATTCCCGGCAAGACCTGGGACCCGACGCTGATTCGCGTCGCGGCCTGAGGTCTGCCGCACACAGAACCAC
GAATTCACTGGAGCCAATCA

Downstream 100 bases:

>100_bases
CCCGCCGAACGGAGCCACTGACATGACCCGACCCCAGGCAGTACCGACCGTCCAGGTCGACAACGAACGTGTGGCCGTGA
CCGAATGGCGCTTCGCCAAG

Product: GABA aminotransferase, PLP-dependent

Products: NA

Alternate protein names: GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MKNAELVRRKDAATPRGVGVMCNFYADRAENSEIWDVEGKRYIDFAAGIAVLNTGHRHPRLVAALQAQLNRFTHTAYQIV
PYASYVELAEKINQRAPGRSARKTAFFTTGAEAVENAVKIARAATGRPGVIAFSGGFHGRTMMGMALTGKVAPYKTGFGP
FPGEVFHAPYPSALHGVTVEDSRRALEHLFKADIDPRRVAAIIFEPVQGEGGFNVAPPEFVRMLRAVCDEHGILLVADEV
QTGFGRTGKLFAMEHYDVAPDLTTMAKSLAGGMPLSAVCGRAEIMDAPAPGGLGGTYAGNPLAVASALAVLDVLESENLI
ARGAELGERLTARLNSLKPRVPQIAEVRGIGAMVAVEFRRADGSPDAEFTREVQNRALERGLLLLSCGVYGNVIRFLFPL
TIQDAVMNEGLDILADVLTR

Sequences:

>Translated_420_residues
MKNAELVRRKDAATPRGVGVMCNFYADRAENSEIWDVEGKRYIDFAAGIAVLNTGHRHPRLVAALQAQLNRFTHTAYQIV
PYASYVELAEKINQRAPGRSARKTAFFTTGAEAVENAVKIARAATGRPGVIAFSGGFHGRTMMGMALTGKVAPYKTGFGP
FPGEVFHAPYPSALHGVTVEDSRRALEHLFKADIDPRRVAAIIFEPVQGEGGFNVAPPEFVRMLRAVCDEHGILLVADEV
QTGFGRTGKLFAMEHYDVAPDLTTMAKSLAGGMPLSAVCGRAEIMDAPAPGGLGGTYAGNPLAVASALAVLDVLESENLI
ARGAELGERLTARLNSLKPRVPQIAEVRGIGAMVAVEFRRADGSPDAEFTREVQNRALERGLLLLSCGVYGNVIRFLFPL
TIQDAVMNEGLDILADVLTR
>Mature_420_residues
MKNAELVRRKDAATPRGVGVMCNFYADRAENSEIWDVEGKRYIDFAAGIAVLNTGHRHPRLVAALQAQLNRFTHTAYQIV
PYASYVELAEKINQRAPGRSARKTAFFTTGAEAVENAVKIARAATGRPGVIAFSGGFHGRTMMGMALTGKVAPYKTGFGP
FPGEVFHAPYPSALHGVTVEDSRRALEHLFKADIDPRRVAAIIFEPVQGEGGFNVAPPEFVRMLRAVCDEHGILLVADEV
QTGFGRTGKLFAMEHYDVAPDLTTMAKSLAGGMPLSAVCGRAEIMDAPAPGGLGGTYAGNPLAVASALAVLDVLESENLI
ARGAELGERLTARLNSLKPRVPQIAEVRGIGAMVAVEFRRADGSPDAEFTREVQNRALERGLLLLSCGVYGNVIRFLFPL
TIQDAVMNEGLDILADVLTR

Specific function: Involved in the breakdown of putrescine via transamination of gamma-aminobutyrate [H]

COG id: COG0160

COG function: function code E; 4-aminobutyrate aminotransferase and related aminotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=399, Percent_Identity=32.5814536340852, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI13994255, Length=418, Percent_Identity=29.9043062200957, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI37574042, Length=450, Percent_Identity=29.5555555555556, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI284507298, Length=317, Percent_Identity=33.1230283911672, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI226442705, Length=449, Percent_Identity=29.3986636971047, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI188536080, Length=462, Percent_Identity=28.1385281385281, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI38679950, Length=462, Percent_Identity=28.1385281385281, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI38679946, Length=462, Percent_Identity=28.1385281385281, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI226442709, Length=390, Percent_Identity=30.2564102564103, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI24119277, Length=393, Percent_Identity=28.2442748091603, Blast_Score=136, Evalue=3e-32,
Organism=Escherichia coli, GI1787560, Length=420, Percent_Identity=68.8095238095238, Blast_Score=579, Evalue=1e-166,
Organism=Escherichia coli, GI1789016, Length=418, Percent_Identity=56.9377990430622, Blast_Score=495, Evalue=1e-141,
Organism=Escherichia coli, GI1789759, Length=404, Percent_Identity=35.6435643564356, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI1788044, Length=400, Percent_Identity=37.25, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI145693181, Length=378, Percent_Identity=35.7142857142857, Blast_Score=195, Evalue=5e-51,
Organism=Escherichia coli, GI1786991, Length=343, Percent_Identity=34.1107871720117, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1786349, Length=347, Percent_Identity=31.700288184438, Blast_Score=145, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI25144271, Length=406, Percent_Identity=32.2660098522167, Blast_Score=193, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI32564660, Length=433, Percent_Identity=31.8706697459584, Blast_Score=184, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI71992977, Length=453, Percent_Identity=30.4635761589404, Blast_Score=167, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI25144274, Length=292, Percent_Identity=31.1643835616438, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17541228, Length=426, Percent_Identity=25.8215962441315, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71981843, Length=151, Percent_Identity=32.4503311258278, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323470, Length=418, Percent_Identity=32.0574162679426, Blast_Score=207, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6324432, Length=400, Percent_Identity=32.75, Blast_Score=199, Evalue=5e-52,
Organism=Saccharomyces cerevisiae, GI6324386, Length=402, Percent_Identity=25.6218905472637, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321456, Length=359, Percent_Identity=27.0194986072423, Blast_Score=96, Evalue=7e-21,
Organism=Drosophila melanogaster, GI21357415, Length=402, Percent_Identity=34.8258706467662, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI28574759, Length=417, Percent_Identity=32.8537170263789, Blast_Score=193, Evalue=2e-49,
Organism=Drosophila melanogaster, GI161085790, Length=400, Percent_Identity=32.75, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI21356575, Length=418, Percent_Identity=30.1435406698565, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI281366494, Length=396, Percent_Identity=27.7777777777778, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24667139, Length=396, Percent_Identity=27.7777777777778, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24667143, Length=396, Percent_Identity=27.7777777777778, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004632
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =2.6.1.19 [H]

Molecular weight: Translated: 45158; Mature: 45158

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNAELVRRKDAATPRGVGVMCNFYADRAENSEIWDVEGKRYIDFAAGIAVLNTGHRHPR
CCCHHHHHHHCCCCCCCCEEEEEEECCCCCCCCEEECCCCEEEHHHHHHHHCCCCCCCHH
LVAALQAQLNRFTHTAYQIVPYASYVELAEKINQRAPGRSARKTAFFTTGAEAVENAVKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHH
ARAATGRPGVIAFSGGFHGRTMMGMALTGKVAPYKTGFGPFPGEVFHAPYPSALHGVTVE
HHHCCCCCCEEEEECCCCCCEEECEEEECCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCH
DSRRALEHLFKADIDPRRVAAIIFEPVQGEGGFNVAPPEFVRMLRAVCDEHGILLVADEV
HHHHHHHHHHHCCCCHHHEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCC
QTGFGRTGKLFAMEHYDVAPDLTTMAKSLAGGMPLSAVCGRAEIMDAPAPGGLGGTYAGN
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCHHHHCCCHHEECCCCCCCCCCCCCCC
PLAVASALAVLDVLESENLIARGAELGERLTARLNSLKPRVPQIAEVRGIGAMVAVEFRR
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCEEEEEEEEC
ADGSPDAEFTREVQNRALERGLLLLSCGVYGNVIRFLFPLTIQDAVMNEGLDILADVLTR
CCCCCCHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure
MKNAELVRRKDAATPRGVGVMCNFYADRAENSEIWDVEGKRYIDFAAGIAVLNTGHRHPR
CCCHHHHHHHCCCCCCCCEEEEEEECCCCCCCCEEECCCCEEEHHHHHHHHCCCCCCCHH
LVAALQAQLNRFTHTAYQIVPYASYVELAEKINQRAPGRSARKTAFFTTGAEAVENAVKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHH
ARAATGRPGVIAFSGGFHGRTMMGMALTGKVAPYKTGFGPFPGEVFHAPYPSALHGVTVE
HHHCCCCCCEEEEECCCCCCEEECEEEECCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCH
DSRRALEHLFKADIDPRRVAAIIFEPVQGEGGFNVAPPEFVRMLRAVCDEHGILLVADEV
HHHHHHHHHHHCCCCHHHEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCC
QTGFGRTGKLFAMEHYDVAPDLTTMAKSLAGGMPLSAVCGRAEIMDAPAPGGLGGTYAGN
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCHHHHCCCHHEECCCCCCCCCCCCCCC
PLAVASALAVLDVLESENLIARGAELGERLTARLNSLKPRVPQIAEVRGIGAMVAVEFRR
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCEEEEEEEEC
ADGSPDAEFTREVQNRALERGLLLLSCGVYGNVIRFLFPLTIQDAVMNEGLDILADVLTR
CCCCCCHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]