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The map label for this gene is azoR1 [H]

Identifier: 94310532

GI number: 94310532

Start: 1714908

End: 1715531

Strand: Reverse

Name: azoR1 [H]

Synonym: Rmet_1590

Alternate gene names: 94310532

Gene position: 1715531-1714908 (Counterclockwise)

Preceding gene: 94310534

Following gene: 94310531

Centisome position: 43.67

GC content: 65.87

Gene sequence:

>624_bases
ATGAACATCCTGCAAATCGATTCGAGCGTACTGGGCGACAACTCCGTATCGCGTTCCCTGACCGCCAGCATCGTGGCCGA
CCTCGTTGCCAAGAACCCAGGCGCCAAGGTAACCGTGCGCGATCTGGACAAGGAAGCCCCCGCCCACCTCGGCAACCACC
TGCTGCCGGTGCTGGGTGGCCCGAAGGACGGCCTGAACGCCGTGCAGCAAGCCGAACTCGAGCGCACTGAAACGTTCCTG
GCCGAATTCATGGCTGCCGATGTGCTGGTGATTGGCGTGCCGCAGTACAACTTCAGCATCCCGAGCCAGCTCAAGGCCTG
GATCGACCGCATCGCGCAAGCCGGCCGCACCTTCCGCTACACCGCCAACGGCCCTGAAGGCCTGGCAGTCGGCAAGCGCG
TGATCGTGGTGTCGTCGCGAGGCGGCGTGCGTCAGGACGCCAACACGCTGGACCTGCACGAAGTCACCATCGATGCGGTG
TTGCGCTTCCTCGGTATCACCGACATCAGCATCGTCCGCGCTCACGGTCTGGCCATGGGCCCCGACGCGCGCGAAGCCGG
TCTCACGACCGCCCGCTCGCAGATTGCCGCGCTCAACGACGCCGCGCTGCGCGCCGCTGCCTGA

Upstream 100 bases:

>100_bases
CAGCGATAGAACAATCATTTGCATTTTGCCTCACTACCGGCACCAATGGGGAACCTCTATCTTTCAGCCATCCAAACCGA
ATTCCCAAGTGGAGCCAGAC

Downstream 100 bases:

>100_bases
GCCTGACAGTATCTCCCTCTCCGTGTAGTTCCCGGCGCCCTCCCCCGGTGCCGGCGATCCTTAAGACAGGTGCCTCCCCC
AGGCACCTGTTTTTTTGTGG

Product: FMN-dependent NADH-azoreductase

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase 1; FMN-dependent NADH-azo compound oxidoreductase 1 [H]

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MNILQIDSSVLGDNSVSRSLTASIVADLVAKNPGAKVTVRDLDKEAPAHLGNHLLPVLGGPKDGLNAVQQAELERTETFL
AEFMAADVLVIGVPQYNFSIPSQLKAWIDRIAQAGRTFRYTANGPEGLAVGKRVIVVSSRGGVRQDANTLDLHEVTIDAV
LRFLGITDISIVRAHGLAMGPDAREAGLTTARSQIAALNDAALRAAA

Sequences:

>Translated_207_residues
MNILQIDSSVLGDNSVSRSLTASIVADLVAKNPGAKVTVRDLDKEAPAHLGNHLLPVLGGPKDGLNAVQQAELERTETFL
AEFMAADVLVIGVPQYNFSIPSQLKAWIDRIAQAGRTFRYTANGPEGLAVGKRVIVVSSRGGVRQDANTLDLHEVTIDAV
LRFLGITDISIVRAHGLAMGPDAREAGLTTARSQIAALNDAALRAAA
>Mature_207_residues
MNILQIDSSVLGDNSVSRSLTASIVADLVAKNPGAKVTVRDLDKEAPAHLGNHLLPVLGGPKDGLNAVQQAELERTETFL
AEFMAADVLVIGVPQYNFSIPSQLKAWIDRIAQAGRTFRYTANGPEGLAVGKRVIVVSSRGGVRQDANTLDLHEVTIDAV
LRFLGITDISIVRAHGLAMGPDAREAGLTTARSQIAALNDAALRAAA

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity [H]

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family [H]

Homologues:

Organism=Escherichia coli, GI1787680, Length=197, Percent_Identity=39.0862944162437, Blast_Score=133, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680
- InterPro:   IPR023048 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 3.1.4.14

Molecular weight: Translated: 21854; Mature: 21854

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILQIDSSVLGDNSVSRSLTASIVADLVAKNPGAKVTVRDLDKEAPAHLGNHLLPVLGG
CCEEECCCHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEHHCCCCCHHHHCCCCEECCCC
PKDGLNAVQQAELERTETFLAEFMAADVLVIGVPQYNFSIPSQLKAWIDRIAQAGRTFRY
CCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEE
TANGPEGLAVGKRVIVVSSRGGVRQDANTLDLHEVTIDAVLRFLGITDISIVRAHGLAMG
ECCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCC
PDAREAGLTTARSQIAALNDAALRAAA
CCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNILQIDSSVLGDNSVSRSLTASIVADLVAKNPGAKVTVRDLDKEAPAHLGNHLLPVLGG
CCEEECCCHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEHHCCCCCHHHHCCCCEECCCC
PKDGLNAVQQAELERTETFLAEFMAADVLVIGVPQYNFSIPSQLKAWIDRIAQAGRTFRY
CCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEE
TANGPEGLAVGKRVIVVSSRGGVRQDANTLDLHEVTIDAVLRFLGITDISIVRAHGLAMG
ECCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCC
PDAREAGLTTARSQIAALNDAALRAAA
CCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA