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The map label for this gene is hypE2 [H]

Identifier: 94310482

GI number: 94310482

Start: 1660741

End: 1661790

Strand: Direct

Name: hypE2 [H]

Synonym: Rmet_1540

Alternate gene names: 94310482

Gene position: 1660741-1661790 (Clockwise)

Preceding gene: 94310481

Following gene: 94310483

Centisome position: 42.28

GC content: 66.67

Gene sequence:

>1050_bases
ATGAGCGAAGTGAAGAAAGGCTATATCCGGCCGCTGGATTTCAAGCACGGCCGCATTGACATGAGCCACGGCGGCGGCGG
GCGCGCCATGGCGCAGTTGATCGACGAGCTGTTCCTGCGCGCTTTCGACAATGAGTGGCTGCGCCAGTTGAACGATCAGG
CCTGTTTCGCCGTCACCACGGGGCGCATGGTGATGGCCACTGATGCCCACGTGGTGTCGCCGCTGTTCTTCCCCGGCGGC
GACATCGGCGGCCTGTCGGTGCATGGCACCATCAACGACGTGGCCATGGCCGGCGCCCGGCCGCTCTACCTCGCCGCCAG
CTTCGTCATCGAGGAGGGTTTTCCGCTGGCGGACCTGCGGCGCATCGTGGACTCCATGGCCCAGGCGTCGCAGGAAGCGC
GGGTGCCCATCATCACCGGTGATACCAAGGTCGTCGAGCAGGGCAAGGGCGACGGTGTTTTCATCACTACGACCGGCGTC
GGCGTCGTGCCCGAGGGCGTGAGTATCAGCGGCGAGCGGGCCCGGCCGGGCGACCGCATCATCGTCTCCGGTGCCATCGG
CGACCACGGCATGGCCATCATGTCGCAGCGGGAGAACCTGCGCTTCGACACCGAGATCGTCTCCGACACCGCGGCCCTGC
ACGGCCTGGTGACGGCGATGGTGATGGCGGTGCCCGACATCCACGTGCTGCGCGATCCCACCCGCGGCGGCGTCGCCACC
ACGCTGAACGAGATCGCCCGGCAGGCCGGCGTGGGCATGATGCTGGACGAGGCGGCGATTCCGGTGAATCCGCAGGTGGC
GGCCGCCTGCGAATTCCTTGGCCTGGATCCGCTTTACGTCGCCAACGAGGGCAAGCTCGTCGCCATCTGCGCGGCGGGCG
ATGCAGATCGACTGCTGGAAGTGATGCGTGCCCATCCCCTGGGGGCCCGCGCCGCCGTCATCGGCGAAGTCACCGAAGAT
CCTCATCACTTCGTGCAGATGAAGACCGCCTTCGGCGGACGGCGCATCGTCGATTGGCTCTCCGGTGAGCAGTTGCCGAG
AATATGTTGA

Upstream 100 bases:

>100_bases
GACATCGAAGTGGTGGTGGCAGCTTGATGAATTTAACCGCAGAGACGCAGAGGCGCAGAGTTTTCTCCGTGTCTCGGCGC
CTCTGCGGTGAGGATCGACG

Downstream 100 bases:

>100_bases
GAGGTTGATTTTGAAACGCAGAGACGCCGAGACGCGGAGGAATTCAGAACAGGTTTTCTCGGCGCCTCGGCGCCTCGGCG
TTTAATGCAGCGTGATTCAT

Product: carbamoyl phosphate phosphatase, hydrogenase 3 maturation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MSEVKKGYIRPLDFKHGRIDMSHGGGGRAMAQLIDELFLRAFDNEWLRQLNDQACFAVTTGRMVMATDAHVVSPLFFPGG
DIGGLSVHGTINDVAMAGARPLYLAASFVIEEGFPLADLRRIVDSMAQASQEARVPIITGDTKVVEQGKGDGVFITTTGV
GVVPEGVSISGERARPGDRIIVSGAIGDHGMAIMSQRENLRFDTEIVSDTAALHGLVTAMVMAVPDIHVLRDPTRGGVAT
TLNEIARQAGVGMMLDEAAIPVNPQVAAACEFLGLDPLYVANEGKLVAICAAGDADRLLEVMRAHPLGARAAVIGEVTED
PHHFVQMKTAFGGRRIVDWLSGEQLPRIC

Sequences:

>Translated_349_residues
MSEVKKGYIRPLDFKHGRIDMSHGGGGRAMAQLIDELFLRAFDNEWLRQLNDQACFAVTTGRMVMATDAHVVSPLFFPGG
DIGGLSVHGTINDVAMAGARPLYLAASFVIEEGFPLADLRRIVDSMAQASQEARVPIITGDTKVVEQGKGDGVFITTTGV
GVVPEGVSISGERARPGDRIIVSGAIGDHGMAIMSQRENLRFDTEIVSDTAALHGLVTAMVMAVPDIHVLRDPTRGGVAT
TLNEIARQAGVGMMLDEAAIPVNPQVAAACEFLGLDPLYVANEGKLVAICAAGDADRLLEVMRAHPLGARAAVIGEVTED
PHHFVQMKTAFGGRRIVDWLSGEQLPRIC
>Mature_348_residues
SEVKKGYIRPLDFKHGRIDMSHGGGGRAMAQLIDELFLRAFDNEWLRQLNDQACFAVTTGRMVMATDAHVVSPLFFPGGD
IGGLSVHGTINDVAMAGARPLYLAASFVIEEGFPLADLRRIVDSMAQASQEARVPIITGDTKVVEQGKGDGVFITTTGVG
VVPEGVSISGERARPGDRIIVSGAIGDHGMAIMSQRENLRFDTEIVSDTAALHGLVTAMVMAVPDIHVLRDPTRGGVATT
LNEIARQAGVGMMLDEAAIPVNPQVAAACEFLGLDPLYVANEGKLVAICAAGDADRLLEVMRAHPLGARAAVIGEVTEDP
HHFVQMKTAFGGRRIVDWLSGEQLPRIC

Specific function: Unknown

COG id: COG0309

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypE family [H]

Homologues:

Organism=Escherichia coli, GI226510967, Length=337, Percent_Identity=44.8071216617211, Blast_Score=290, Evalue=8e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR011854
- InterPro:   IPR016188 [H]

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C [H]

EC number: NA

Molecular weight: Translated: 37187; Mature: 37056

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVKKGYIRPLDFKHGRIDMSHGGGGRAMAQLIDELFLRAFDNEWLRQLNDQACFAVTT
CCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCHHHHHHHCCCEEEEEEC
GRMVMATDAHVVSPLFFPGGDIGGLSVHGTINDVAMAGARPLYLAASFVIEEGFPLADLR
CCEEEEECCHHHCEEECCCCCCCCEEEECCHHHHHHCCCCHHHHHHHHHHHCCCCHHHHH
RIVDSMAQASQEARVPIITGDTKVVEQGKGDGVFITTTGVGVVPEGVSISGERARPGDRI
HHHHHHHHHHHHCCCCEEECCHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEE
IVSGAIGDHGMAIMSQRENLRFDTEIVSDTAALHGLVTAMVMAVPDIHVLRDPTRGGVAT
EEEECCCCCCHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHH
TLNEIARQAGVGMMLDEAAIPVNPQVAAACEFLGLDPLYVANEGKLVAICAAGDADRLLE
HHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHCCCCCEEEECCCCEEEEEECCCHHHHHH
VMRAHPLGARAAVIGEVTEDPHHFVQMKTAFGGRRIVDWLSGEQLPRIC
HHHCCCCCCCEEEEECCCCCCHHHEEEHHHCCCCHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SEVKKGYIRPLDFKHGRIDMSHGGGGRAMAQLIDELFLRAFDNEWLRQLNDQACFAVTT
CCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCHHHHHHHCCCEEEEEEC
GRMVMATDAHVVSPLFFPGGDIGGLSVHGTINDVAMAGARPLYLAASFVIEEGFPLADLR
CCEEEEECCHHHCEEECCCCCCCCEEEECCHHHHHHCCCCHHHHHHHHHHHCCCCHHHHH
RIVDSMAQASQEARVPIITGDTKVVEQGKGDGVFITTTGVGVVPEGVSISGERARPGDRI
HHHHHHHHHHHHCCCCEEECCHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEE
IVSGAIGDHGMAIMSQRENLRFDTEIVSDTAALHGLVTAMVMAVPDIHVLRDPTRGGVAT
EEEECCCCCCHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHH
TLNEIARQAGVGMMLDEAAIPVNPQVAAACEFLGLDPLYVANEGKLVAICAAGDADRLLE
HHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHCCCCCEEEECCCCEEEEEECCCHHHHHH
VMRAHPLGARAAVIGEVTEDPHHFVQMKTAFGGRRIVDWLSGEQLPRIC
HHHCCCCCCCEEEEECCCCCCHHHEEEHHHCCCCHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8352644; 12948488; 8021224 [H]