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The map label for this gene is int [H]

Identifier: 94310407

GI number: 94310407

Start: 1585594

End: 1586850

Strand: Direct

Name: int [H]

Synonym: Rmet_1465

Alternate gene names: 94310407

Gene position: 1585594-1586850 (Clockwise)

Preceding gene: 94310405

Following gene: 94310408

Centisome position: 40.37

GC content: 61.97

Gene sequence:

>1257_bases
ATGCTGACCGACACAGCACTGCGCAATCTGAAGCCTAAGTCATTGACTTATAAGGTTTCTGACCGGGACGGGATGTACGT
GACGGTATCGCCCGCAAATACCGTCACCTTCCGCTACGACTACCGTCTCAACGGCCGCCGCGAGACCTTGACCATCGGCC
GCTACGGTGCAGGCGGCATTTCGCTGGCAATGGCGCGTGAACTGCTTCTGGAGGCGCGCAAGGCCGTTCTCCAGGGCATA
TCGCCCGCGCTGGAGAAGCAACGCGACAAGCGCCGGGTTCGCGCCATCAAGACCTTCGGCGCGGCGATGGAGACGTATCT
TGCCCACACGAGATGGGCCGACAGCACCCGCGCCGTGCGCAAGCACATCATCGACCGGGACATCCTGCCGGTTTTCCAGA
ATCGGCTGCTGACCGAGATCCAGGCCGAAGACCTTCGGATTCTGTGCAACAAGGTCAAGGAGCGCGGGGCACCAGCCACC
GCGGTGCAGATCCGGGACATCGTGAAGCAGGTCTACGTCTACGCCATCGCCCACGGTGAGAAGGTGGTCAACCCCGCCGA
CAGCGTGGGGCCGTCCTCGATCGCCACCTTCGTGCCGAAGGATCGTGCCCTCTCGCCGCTGGAAATCCGTTTGATGGTGC
AGCAGTTGGAGACGGTGGCGACCTATCCGACCATCAAGCTGGCGCTGCGCCTGATCCTGCTGACGCTGGTGCGCAAGAGC
GAACTGATCCAGGCCACTTGGGATGAAGTCGATTTCGAGCGCAAGACCTGGACGATTCCGAAGCAGCGGATGAAGGGTCG
CAATCCGCACGTGGTCTATCTGTCGCGCCAGGCGCTCGACATCTTCGTGACGCTGCACGCCTGTGCGGCTGGCTCAAAGT
TCGTTTTCCCTTCTCGCTACGATGCAGAGCGGTGCATGTCCAATGCGACCTTGAATCGCATCACGATGCTGGTGGCAGAA
GGTGCCAAGGCCAAAGATCTGCCGCTACAGCCGTTCACCGTCCACGATCTGCGCCGCACCGGCTCGACGCTGCTGAACGA
AATCGGCTTCAACCGCGACTGGATTGAGAAGTGCCTGGCGCACGAGGAGGGGCGTTCCTCGCGTTCGGTCTACAACAAGG
CCGAATACGCCGAGCAGCGGCGCCACATGCTGCAAGAGTGGGCCAACCTGGTCGATGCCTGGGGTGGCGGACAGACTTAC
GTGCCGAAGCTGATGCCGGAGAACGTGGTCGTGCCGGCGTTGAGCGCGATCGCGTAG

Upstream 100 bases:

>100_bases
AGCCTTCTCGGGAAAGCTTATTGCATGCGGCTTTCCGGGCATCAGTGGGTCTCCTGGTCCTTGACGGTAAAAGCCGTCGC
AAAACAGGAGGTAAACCTCG

Downstream 100 bases:

>100_bases
GAGGGCCGCATCATGGGTTGCCTTGAATCGGTCTTTGCCGGGTTGTGCCAGGTTTGTTATTTCGAGGAAGCGATCACTTT
GAGTGATCGAAAACGAGGAA

Product: tyrosine-based site-specific recombinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MLTDTALRNLKPKSLTYKVSDRDGMYVTVSPANTVTFRYDYRLNGRRETLTIGRYGAGGISLAMARELLLEARKAVLQGI
SPALEKQRDKRRVRAIKTFGAAMETYLAHTRWADSTRAVRKHIIDRDILPVFQNRLLTEIQAEDLRILCNKVKERGAPAT
AVQIRDIVKQVYVYAIAHGEKVVNPADSVGPSSIATFVPKDRALSPLEIRLMVQQLETVATYPTIKLALRLILLTLVRKS
ELIQATWDEVDFERKTWTIPKQRMKGRNPHVVYLSRQALDIFVTLHACAAGSKFVFPSRYDAERCMSNATLNRITMLVAE
GAKAKDLPLQPFTVHDLRRTGSTLLNEIGFNRDWIEKCLAHEEGRSSRSVYNKAEYAEQRRHMLQEWANLVDAWGGGQTY
VPKLMPENVVVPALSAIA

Sequences:

>Translated_418_residues
MLTDTALRNLKPKSLTYKVSDRDGMYVTVSPANTVTFRYDYRLNGRRETLTIGRYGAGGISLAMARELLLEARKAVLQGI
SPALEKQRDKRRVRAIKTFGAAMETYLAHTRWADSTRAVRKHIIDRDILPVFQNRLLTEIQAEDLRILCNKVKERGAPAT
AVQIRDIVKQVYVYAIAHGEKVVNPADSVGPSSIATFVPKDRALSPLEIRLMVQQLETVATYPTIKLALRLILLTLVRKS
ELIQATWDEVDFERKTWTIPKQRMKGRNPHVVYLSRQALDIFVTLHACAAGSKFVFPSRYDAERCMSNATLNRITMLVAE
GAKAKDLPLQPFTVHDLRRTGSTLLNEIGFNRDWIEKCLAHEEGRSSRSVYNKAEYAEQRRHMLQEWANLVDAWGGGQTY
VPKLMPENVVVPALSAIA
>Mature_418_residues
MLTDTALRNLKPKSLTYKVSDRDGMYVTVSPANTVTFRYDYRLNGRRETLTIGRYGAGGISLAMARELLLEARKAVLQGI
SPALEKQRDKRRVRAIKTFGAAMETYLAHTRWADSTRAVRKHIIDRDILPVFQNRLLTEIQAEDLRILCNKVKERGAPAT
AVQIRDIVKQVYVYAIAHGEKVVNPADSVGPSSIATFVPKDRALSPLEIRLMVQQLETVATYPTIKLALRLILLTLVRKS
ELIQATWDEVDFERKTWTIPKQRMKGRNPHVVYLSRQALDIFVTLHACAAGSKFVFPSRYDAERCMSNATLNRITMLVAE
GAKAKDLPLQPFTVHDLRRTGSTLLNEIGFNRDWIEKCLAHEEGRSSRSVYNKAEYAEQRRHMLQEWANLVDAWGGGQTY
VPKLMPENVVVPALSAIA

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=418, Percent_Identity=31.3397129186603, Blast_Score=168, Evalue=5e-43,
Organism=Escherichia coli, GI145693166, Length=402, Percent_Identity=32.089552238806, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI1788690, Length=397, Percent_Identity=29.4710327455919, Blast_Score=159, Evalue=5e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 47273; Mature: 47273

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTDTALRNLKPKSLTYKVSDRDGMYVTVSPANTVTFRYDYRLNGRRETLTIGRYGAGGI
CCCHHHHHCCCCCCEEEEECCCCCEEEEECCCCEEEEEEEEEECCCEEEEEEECCCCCHH
SLAMARELLLEARKAVLQGISPALEKQRDKRRVRAIKTFGAAMETYLAHTRWADSTRAVR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
KHIIDRDILPVFQNRLLTEIQAEDLRILCNKVKERGAPATAVQIRDIVKQVYVYAIAHGE
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
KVVNPADSVGPSSIATFVPKDRALSPLEIRLMVQQLETVATYPTIKLALRLILLTLVRKS
HHCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
ELIQATWDEVDFERKTWTIPKQRMKGRNPHVVYLSRQALDIFVTLHACAAGSKFVFPSRY
HHHHHHHHHCCCCCCEECCCHHHHCCCCCCEEEEECCCEEHHHHHHHHHCCCCCCCCCCC
DAERCMSNATLNRITMLVAEGAKAKDLPLQPFTVHDLRRTGSTLLNEIGFNRDWIEKCLA
CHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
HEEGRSSRSVYNKAEYAEQRRHMLQEWANLVDAWGGGQTYVPKLMPENVVVPALSAIA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MLTDTALRNLKPKSLTYKVSDRDGMYVTVSPANTVTFRYDYRLNGRRETLTIGRYGAGGI
CCCHHHHHCCCCCCEEEEECCCCCEEEEECCCCEEEEEEEEEECCCEEEEEEECCCCCHH
SLAMARELLLEARKAVLQGISPALEKQRDKRRVRAIKTFGAAMETYLAHTRWADSTRAVR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
KHIIDRDILPVFQNRLLTEIQAEDLRILCNKVKERGAPATAVQIRDIVKQVYVYAIAHGE
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
KVVNPADSVGPSSIATFVPKDRALSPLEIRLMVQQLETVATYPTIKLALRLILLTLVRKS
HHCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
ELIQATWDEVDFERKTWTIPKQRMKGRNPHVVYLSRQALDIFVTLHACAAGSKFVFPSRY
HHHHHHHHHCCCCCCEECCCHHHHCCCCCCEEEEECCCEEHHHHHHHHHCCCCCCCCCCC
DAERCMSNATLNRITMLVAEGAKAKDLPLQPFTVHDLRRTGSTLLNEIGFNRDWIEKCLA
CHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
HEEGRSSRSVYNKAEYAEQRRHMLQEWANLVDAWGGGQTYVPKLMPENVVVPALSAIA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7511583; 9278503; 9205837; 7524073 [H]