Click here to switch to the map view.

The map label for this gene is lysR [H]

Identifier: 94310330

GI number: 94310330

Start: 1497449

End: 1498537

Strand: Reverse

Name: lysR [H]

Synonym: Rmet_1388

Alternate gene names: 94310330

Gene position: 1498537-1497449 (Counterclockwise)

Preceding gene: 94310333

Following gene: 94310329

Centisome position: 38.15

GC content: 62.99

Gene sequence:

>1089_bases
TTGAATGCATCGAATTGTAGTGAGACGTTGCAGTGCGGCAAAGCCAAGGCTGGTCACTTGATCTTTTACTTTTGCACCAC
TAATCTTGCCCAACTTGACCTCGACTTATGTATCAACCGGCGAACATCCGTGGCTCAATTCAAGCAGTTGGAGACCTTTG
TATCGGTCGCATCACGTGGCAGCCTATCTGCCGCCGCGGCCGCGGAGGGCGTCGCGCCCGCGATCATCGGGCGTCGCATC
GATGCGCTCGAAGAACGGCTCGGCGTGAAGTTGCTGGTACGCACCACACGCAAGATCACCCTGACGTTTGAAGGCTCGGC
GTTCCTGGAGGATTGCCAGCGCATCCTCAATGACCTTCACAATGCCGAAGCCAGCGTCTCCGCCGGCGGCGTCAAGGCCA
GCGGCCACCTGCGCATCACCGCGCCGGCCGGCTTCGGCCGCAAGCACGTGGCACCGCTGGTACCCCTGTTCATCGAATCG
CATCCTGACGTCACGCTCACGCTCGATTTATCCGACCGCGTTGTCGATCTGGTCAACGAAGGCTTCGACTGCGCGATCCG
GCTTGGCGACCTGCCCGATTCGAGCCTGGTCTCCATCCGGCTGGCGGAAAACCGCCGCGTCGTCGTCGCCTCCCCCGAAT
ACCTGGCGCGTGCCGGCACGCCGACACAGCCCGAGGAACTGGCACGTCACAATTGTCTGGCCTTCGGCGCCAGCGCCAAC
GTTCAGCGCGGCTGGGTCTTCCAGCAGGACGGCCGGGCCACTACCGTCAAGGTAGGCGGCAACATGGAATGCACCGACGG
TGCCGTGCTCCACGAGTGGTGCCTCGGCGGCTACGGACTCGCCTGGCGTTCATGGTGGGAAGTCGGCCAGGAAATCGCCA
GCGGCCGACTTGTAACCGTGCTCGACGAATTCCAGGCGCCGCCGATCGGCATCCACGCAGTCTTTCCGCAACGCAAGCAC
CTGCCGCTGCGCGTCCGCCTGTTTATCGACCACCTCAAGAACACCTACGGCAACCCCGCCTACTGGCGCCGCGCCGAAAT
GGCGCAACCCAGCACAAACGCCGGCGTCGATATCACCGCACCTGCCTAG

Upstream 100 bases:

>100_bases
TGACCGAATACACGTCGAACAGCGTGCCGTAAGCATCGAAAACTACCGCGCGAATCTTGCTCATCTAACGACCGGATCGA
CTGAATGGACCTGTACACGA

Downstream 100 bases:

>100_bases
ATCGATGGCTACCGCCTGCGCCGCTGTCACGATGCGTCTAGTGCTTTCCCTCACGCATTGATATTTCGCAATGGCGCACA
GCGCACTCTGACTACAATCA

Product: transcriptional regulator, LysR-family

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MNASNCSETLQCGKAKAGHLIFYFCTTNLAQLDLDLCINRRTSVAQFKQLETFVSVASRGSLSAAAAAEGVAPAIIGRRI
DALEERLGVKLLVRTTRKITLTFEGSAFLEDCQRILNDLHNAEASVSAGGVKASGHLRITAPAGFGRKHVAPLVPLFIES
HPDVTLTLDLSDRVVDLVNEGFDCAIRLGDLPDSSLVSIRLAENRRVVVASPEYLARAGTPTQPEELARHNCLAFGASAN
VQRGWVFQQDGRATTVKVGGNMECTDGAVLHEWCLGGYGLAWRSWWEVGQEIASGRLVTVLDEFQAPPIGIHAVFPQRKH
LPLRVRLFIDHLKNTYGNPAYWRRAEMAQPSTNAGVDITAPA

Sequences:

>Translated_362_residues
MNASNCSETLQCGKAKAGHLIFYFCTTNLAQLDLDLCINRRTSVAQFKQLETFVSVASRGSLSAAAAAEGVAPAIIGRRI
DALEERLGVKLLVRTTRKITLTFEGSAFLEDCQRILNDLHNAEASVSAGGVKASGHLRITAPAGFGRKHVAPLVPLFIES
HPDVTLTLDLSDRVVDLVNEGFDCAIRLGDLPDSSLVSIRLAENRRVVVASPEYLARAGTPTQPEELARHNCLAFGASAN
VQRGWVFQQDGRATTVKVGGNMECTDGAVLHEWCLGGYGLAWRSWWEVGQEIASGRLVTVLDEFQAPPIGIHAVFPQRKH
LPLRVRLFIDHLKNTYGNPAYWRRAEMAQPSTNAGVDITAPA
>Mature_362_residues
MNASNCSETLQCGKAKAGHLIFYFCTTNLAQLDLDLCINRRTSVAQFKQLETFVSVASRGSLSAAAAAEGVAPAIIGRRI
DALEERLGVKLLVRTTRKITLTFEGSAFLEDCQRILNDLHNAEASVSAGGVKASGHLRITAPAGFGRKHVAPLVPLFIES
HPDVTLTLDLSDRVVDLVNEGFDCAIRLGDLPDSSLVSIRLAENRRVVVASPEYLARAGTPTQPEELARHNCLAFGASAN
VQRGWVFQQDGRATTVKVGGNMECTDGAVLHEWCLGGYGLAWRSWWEVGQEIASGRLVTVLDEFQAPPIGIHAVFPQRKH
LPLRVRLFIDHLKNTYGNPAYWRRAEMAQPSTNAGVDITAPA

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=291, Percent_Identity=37.8006872852234, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1786401, Length=289, Percent_Identity=35.6401384083045, Blast_Score=168, Evalue=5e-43,
Organism=Escherichia coli, GI1789440, Length=310, Percent_Identity=31.6129032258064, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI145693193, Length=284, Percent_Identity=32.0422535211268, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1787589, Length=290, Percent_Identity=30.3448275862069, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1787128, Length=284, Percent_Identity=28.169014084507, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1789639, Length=293, Percent_Identity=29.0102389078498, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1786448, Length=279, Percent_Identity=28.673835125448, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1790011, Length=305, Percent_Identity=25.2459016393443, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788706, Length=179, Percent_Identity=29.0502793296089, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789173, Length=250, Percent_Identity=26, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1787879, Length=155, Percent_Identity=31.6129032258064, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787601, Length=143, Percent_Identity=26.5734265734266, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 39321; Mature: 39321

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNASNCSETLQCGKAKAGHLIFYFCTTNLAQLDLDLCINRRTSVAQFKQLETFVSVASRG
CCCCCHHHHHHCCCCCCCCEEEEEECCCHHHEEHHHHHCCCCHHHHHHHHHHHHHHHCCC
SLSAAAAAEGVAPAIIGRRIDALEERLGVKLLVRTTRKITLTFEGSAFLEDCQRILNDLH
CCCHHHHHCCCHHHHHHHHHHHHHHHCCEEEEEEECEEEEEEECCCHHHHHHHHHHHHHH
NAEASVSAGGVKASGHLRITAPAGFGRKHVAPLVPLFIESHPDVTLTLDLSDRVVDLVNE
CCCCCCCCCCEEECCEEEEEECCCCCCCHHHHHHHHEECCCCCEEEEEECCHHHHHHHCC
GFDCAIRLGDLPDSSLVSIRLAENRRVVVASPEYLARAGTPTQPEELARHNCLAFGASAN
CCEEEEEECCCCCCCEEEEEEECCCEEEEECCHHHHCCCCCCCHHHHHHCCEEEECCCCC
VQRGWVFQQDGRATTVKVGGNMECTDGAVLHEWCLGGYGLAWRSWWEVGQEIASGRLVTV
CCCCEEEECCCCEEEEEECCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEE
LDEFQAPPIGIHAVFPQRKHLPLRVRLFIDHLKNTYGNPAYWRRAEMAQPSTNAGVDITA
EHHHCCCCCEEEEECCCCCCCCEEEHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEEEC
PA
CC
>Mature Secondary Structure
MNASNCSETLQCGKAKAGHLIFYFCTTNLAQLDLDLCINRRTSVAQFKQLETFVSVASRG
CCCCCHHHHHHCCCCCCCCEEEEEECCCHHHEEHHHHHCCCCHHHHHHHHHHHHHHHCCC
SLSAAAAAEGVAPAIIGRRIDALEERLGVKLLVRTTRKITLTFEGSAFLEDCQRILNDLH
CCCHHHHHCCCHHHHHHHHHHHHHHHCCEEEEEEECEEEEEEECCCHHHHHHHHHHHHHH
NAEASVSAGGVKASGHLRITAPAGFGRKHVAPLVPLFIESHPDVTLTLDLSDRVVDLVNE
CCCCCCCCCCEEECCEEEEEECCCCCCCHHHHHHHHEECCCCCEEEEEECCHHHHHHHCC
GFDCAIRLGDLPDSSLVSIRLAENRRVVVASPEYLARAGTPTQPEELARHNCLAFGASAN
CCEEEEEECCCCCCCEEEEEEECCCEEEEECCHHHHCCCCCCCHHHHHHCCEEEECCCCC
VQRGWVFQQDGRATTVKVGGNMECTDGAVLHEWCLGGYGLAWRSWWEVGQEIASGRLVTV
CCCCEEEECCCCEEEEEECCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEE
LDEFQAPPIGIHAVFPQRKHLPLRVRLFIDHLKNTYGNPAYWRRAEMAQPSTNAGVDITA
EHHHCCCCCEEEEECCCCCCCCEEEHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEEEC
PA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]