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The map label for this gene is aceA [H]

Identifier: 94310327

GI number: 94310327

Start: 1493756

End: 1495051

Strand: Direct

Name: aceA [H]

Synonym: Rmet_1385

Alternate gene names: 94310327

Gene position: 1493756-1495051 (Clockwise)

Preceding gene: 94310326

Following gene: 94310331

Centisome position: 38.03

GC content: 62.5

Gene sequence:

>1296_bases
ATGACGTCGCGCCAACAACAGATTGAAGCCCTGCAGAAGGATTGGGACACCAATCCACGCTGGAAGGGTGTGAAGCGCAA
CTTCACCGCCGAAGATGTGGTTCGTCTGCGCGGCTCGGTCCAGATCGAGCACACGCTGGCCCGCCGCGGCGCGGAAAAAC
TCTGGCACCTGCTGAACACCGAGCCGTTCGTCAACACGCTCGGCGCGCTGACGGGCAATCAGGCCATGCAGCAGGTCAAG
GCCGGTCTGAAGGCCATCTACCTGTCGGGCTGGCAAGTCGCTGGCGACGCCAATCTGGCCGGCGAAATGTACCCCGACCA
GTCGCTGTACCCGGCCAACTCGGTGCCAATGGTCGTGAAGCGCATCAACAACACGTTCCAGCGTGCCGACCAGATCCAGT
GGAGCGAAGGCAAGGGCGACATCGACTACTTCGCACCGATCGTGGCTGACGCGGAAGCCGGTTTTGGTGGCGTGCTGAAT
GCCTTCGAACTGATGAAGTCGATGATCGACGCGGGCGCTGCCGGCGTTCACTTCGAAGACCAGCTCGCTTCGGTGAAGAA
GTGCGGGCACATGGGTGGCAAGGTGCTGGTGCCGACCCGCGAGGCTGTCGCCAAGCTGACCGCAGCGCGTCTCGCCGCCG
ACGTCTCGGGCGTGCCGACCCTGGTGATCGCCCGGACCGACGCGGAAGCCGCTGACCTTGTGACTTCCGACGTGGACGAG
CGCGACCAGCCGTTTCTGACCGGCGAGCGTACCGTGGAGGGTTTCTTCCGTTCGAAGCCCGGCATCGAGCAGTCGATCGC
CCGTGCCCTGGCCTATGCCGAGGTGGCTGACCTTGTGTGGTGCGAAACCGGCAAGCCGGACCTCGAGTTCGCCAAGCGCT
TCGCACAAGCCGTGCACGCGAAGTTCCCGGGCAAGCTGTTGGCCTACAACTGCTCGCCATCGTTCAACTGGAAGAAGAAC
CTCGACGACGCAACGATCGCCAAGTTCCAGAAGGAACTCGGCGCGATGGGCTACAAGTTCCAGTTCATCACGTTGGCGGG
CTTCCATTCGCTGAACTACTCGATGTTCAACCTGGCCCACGGCTACGCCCGCAACCAGATGTCGGCATTCGTGGAGTTGC
AGGAAGCGGAATTCGCCGCCGCGGAGAAGGGCTTTACCGCTGTGAAGCACCAGCGCGAAGTGGGTACGGGCTACTTCGAC
GCCGTGACGCAGACCATCGAAGGCGGCCAGTCGTCGACGACGGCGCTCAAGGGATCGACCGAAGACGAACAGTTCTTTGA
AAAGAAGGTGGCCTGA

Upstream 100 bases:

>100_bases
GAGACGCGAAATGCACAGCGTCCGGGTCGCTCGGCAGATGAGTACGGTGGGTGTCACAGAATCCAACGCCAATCGCCTAG
TTGTTATCAGGAGAGTGAAC

Downstream 100 bases:

>100_bases
TCCTGGTTTGAGACAGTAGAAACCTGAGGTTGCAGTACGCAACGCCGCGTCGTCCGGTAGGGGACGGCGCGGCGTTGTCT
TTGGTGCGTGCTGGCCGGCT

Product: isocitrate lyase

Products: NA

Alternate protein names: ICL; Isocitrase; Isocitratase [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MTSRQQQIEALQKDWDTNPRWKGVKRNFTAEDVVRLRGSVQIEHTLARRGAEKLWHLLNTEPFVNTLGALTGNQAMQQVK
AGLKAIYLSGWQVAGDANLAGEMYPDQSLYPANSVPMVVKRINNTFQRADQIQWSEGKGDIDYFAPIVADAEAGFGGVLN
AFELMKSMIDAGAAGVHFEDQLASVKKCGHMGGKVLVPTREAVAKLTAARLAADVSGVPTLVIARTDAEAADLVTSDVDE
RDQPFLTGERTVEGFFRSKPGIEQSIARALAYAEVADLVWCETGKPDLEFAKRFAQAVHAKFPGKLLAYNCSPSFNWKKN
LDDATIAKFQKELGAMGYKFQFITLAGFHSLNYSMFNLAHGYARNQMSAFVELQEAEFAAAEKGFTAVKHQREVGTGYFD
AVTQTIEGGQSSTTALKGSTEDEQFFEKKVA

Sequences:

>Translated_431_residues
MTSRQQQIEALQKDWDTNPRWKGVKRNFTAEDVVRLRGSVQIEHTLARRGAEKLWHLLNTEPFVNTLGALTGNQAMQQVK
AGLKAIYLSGWQVAGDANLAGEMYPDQSLYPANSVPMVVKRINNTFQRADQIQWSEGKGDIDYFAPIVADAEAGFGGVLN
AFELMKSMIDAGAAGVHFEDQLASVKKCGHMGGKVLVPTREAVAKLTAARLAADVSGVPTLVIARTDAEAADLVTSDVDE
RDQPFLTGERTVEGFFRSKPGIEQSIARALAYAEVADLVWCETGKPDLEFAKRFAQAVHAKFPGKLLAYNCSPSFNWKKN
LDDATIAKFQKELGAMGYKFQFITLAGFHSLNYSMFNLAHGYARNQMSAFVELQEAEFAAAEKGFTAVKHQREVGTGYFD
AVTQTIEGGQSSTTALKGSTEDEQFFEKKVA
>Mature_430_residues
TSRQQQIEALQKDWDTNPRWKGVKRNFTAEDVVRLRGSVQIEHTLARRGAEKLWHLLNTEPFVNTLGALTGNQAMQQVKA
GLKAIYLSGWQVAGDANLAGEMYPDQSLYPANSVPMVVKRINNTFQRADQIQWSEGKGDIDYFAPIVADAEAGFGGVLNA
FELMKSMIDAGAAGVHFEDQLASVKKCGHMGGKVLVPTREAVAKLTAARLAADVSGVPTLVIARTDAEAADLVTSDVDER
DQPFLTGERTVEGFFRSKPGIEQSIARALAYAEVADLVWCETGKPDLEFAKRFAQAVHAKFPGKLLAYNCSPSFNWKKNL
DDATIAKFQKELGAMGYKFQFITLAGFHSLNYSMFNLAHGYARNQMSAFVELQEAEFAAAEKGFTAVKHQREVGTGYFDA
VTQTIEGGQSSTTALKGSTEDEQFFEKKVA

Specific function: Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase- positive serine pathway [H]

COG id: COG2224

COG function: function code C; Isocitrate lyase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1790445, Length=435, Percent_Identity=71.0344827586207, Blast_Score=635, Evalue=0.0,
Organism=Escherichia coli, GI1786525, Length=262, Percent_Identity=29.0076335877863, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71982926, Length=413, Percent_Identity=63.4382566585956, Blast_Score=549, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI17561814, Length=413, Percent_Identity=63.4382566585956, Blast_Score=549, Evalue=1e-157,
Organism=Saccharomyces cerevisiae, GI6320908, Length=287, Percent_Identity=35.8885017421603, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6325263, Length=251, Percent_Identity=36.6533864541833, Blast_Score=150, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006254
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.1 [H]

Molecular weight: Translated: 47300; Mature: 47169

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRQQQIEALQKDWDTNPRWKGVKRNFTAEDVVRLRGSVQIEHTLARRGAEKLWHLLNT
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCEEHHHHHHHHHHHHHHHHHCC
EPFVNTLGALTGNQAMQQVKAGLKAIYLSGWQVAGDANLAGEMYPDQSLYPANSVPMVVK
CHHHHHHHHHCCHHHHHHHHHHHHEEEECCEEEECCCCCCCCCCCCCCCCCCCCHHHHHH
RINNTFQRADQIQWSEGKGDIDYFAPIVADAEAGFGGVLNAFELMKSMIDAGAAGVHFED
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHH
QLASVKKCGHMGGKVLVPTREAVAKLTAARLAADVSGVPTLVIARTDAEAADLVTSDVDE
HHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCC
RDQPFLTGERTVEGFFRSKPGIEQSIARALAYAEVADLVWCETGKPDLEFAKRFAQAVHA
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHH
KFPGKLLAYNCSPSFNWKKNLDDATIAKFQKELGAMGYKFQFITLAGFHSLNYSMFNLAH
CCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECHHHCCHHHHHHHH
GYARNQMSAFVELQEAEFAAAEKGFTAVKHQREVGTGYFDAVTQTIEGGQSSTTALKGST
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEECCCC
EDEQFFEKKVA
CHHHHHHHHCC
>Mature Secondary Structure 
TSRQQQIEALQKDWDTNPRWKGVKRNFTAEDVVRLRGSVQIEHTLARRGAEKLWHLLNT
CCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCEEHHHHHHHHHHHHHHHHHCC
EPFVNTLGALTGNQAMQQVKAGLKAIYLSGWQVAGDANLAGEMYPDQSLYPANSVPMVVK
CHHHHHHHHHCCHHHHHHHHHHHHEEEECCEEEECCCCCCCCCCCCCCCCCCCCHHHHHH
RINNTFQRADQIQWSEGKGDIDYFAPIVADAEAGFGGVLNAFELMKSMIDAGAAGVHFED
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHH
QLASVKKCGHMGGKVLVPTREAVAKLTAARLAADVSGVPTLVIARTDAEAADLVTSDVDE
HHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCC
RDQPFLTGERTVEGFFRSKPGIEQSIARALAYAEVADLVWCETGKPDLEFAKRFAQAVHA
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHH
KFPGKLLAYNCSPSFNWKKNLDDATIAKFQKELGAMGYKFQFITLAGFHSLNYSMFNLAH
CCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECHHHCCHHHHHHHH
GYARNQMSAFVELQEAEFAAAEKGFTAVKHQREVGTGYFDAVTQTIEGGQSSTTALKGST
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEECCCC
EDEQFFEKKVA
CHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]