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The map label for this gene is bphI [C]

Identifier: 94310261

GI number: 94310261

Start: 1432779

End: 1433825

Strand: Reverse

Name: bphI [C]

Synonym: Rmet_1319

Alternate gene names: 94310261

Gene position: 1433825-1432779 (Counterclockwise)

Preceding gene: 94310262

Following gene: 94310260

Centisome position: 36.5

GC content: 62.18

Gene sequence:

>1047_bases
ATGAACCTGCAAGGAAAAAAAATCACCGTCCACGACATGACGTTGCGTGACGGCATGCATCCGAAGCGCCATATGATGAC
GCTGGAGCAAATGAAAAGCATCGCCTGCGGCCTGGACGCAGCCGGGGTCCCCCTGATTGAAGTCACGCACGGCGATGGCC
TGGGCGGCTCGTCGGTCAACTATGGCTTTCCGGCCCACAGCGATGAAGAATATCTGGGGGCCGTGATCCCGCTCATGAAG
CAGGCCAAGGTCTCGGCTTTACTGTTGCCGGGCATCGGCACAGTCGACCATCTGAAGATGGCCAAGGAGCTTGGCGTGCA
CACCATCCGGGTGGCTACGCACTGCACCGAGGCTGACGTGTCGGAGCAGCACATTGCCCTGGCGCGCAAGCTTGAGATGG
ACACGGTGGGCTTCCTGATGATGGCGCATATGAACAGTCCTGAAGGGCTGGTCGGGCAGGCAAAGCTGATGGAGTCGTAC
GGCGCCAACTGTGTTTACATTACCGATTCGGCTGGCTACATGCTGCCCGATGATGTCAGGGCCCGCCTTGGCGCGGTGCG
TGAGGCGCTCAAGCCGGAGACGGAACTGGGCTTTCACGGCCACCACAACCTGGCGATGGGGATCGCCAACTCCATTGCGG
CCGTTGAGTGTGGCGCCACCCGCATCGACGCCGCCTCGGCTGGTCTCGGTGCGGGTGCGGGCAACACGCCGATGGAGGTC
CTGGTCGCCGTATGCGACCGAATGGGGATTCAGACCGGAGTGGATGTGTGGGCCATTCAGGACGTGGCCGAAGACCTGGT
TGTGCCGATCATGGACTTCCCCATCCGGATCGACCGCGACTCGCTCACCCTGGGTTACGCCGGGGTCTACGGGTCGTTCC
TGCTGTTTGCCAAGCGCGCCGAGCGAAAGTATGGCGTACCGGCGCGCGAGATCTTGGTCGAGCTTGGGCGCCGGGGCATG
GTCGGTGGCCAGGAAGATATGATTGAAGACACGGCCATCACGCTGGCCAAAGCGCGTGCGTCAAAGGCGCAGAAGGTGGC
GGCATGA

Upstream 100 bases:

>100_bases
GCTGCCGCGGCCCGGACCGCCGAGATGTTTGCCGAAGAGATGATTGCCGGCAATCTCACGCTCGAACCGGTTGTCGCTTG
AGCCGTCAAGGAGAACAGAG

Downstream 100 bases:

>100_bases
AACTGGATCGCACCACCATTGCGCGCCTTGCAGAGCACCTCGAGAACTGCGAGCTCCACGCGCAAGACACGCTCAAGATC
ACCGATGAGCATCCCGAGAT

Product: 4-hyroxy-2-oxovalerate/4-hydroxy-2-oxopentanoic acid aldolase

Products: NA

Alternate protein names: HOA 1; 4-hydroxy-2-keto-pentanoic acid aldolase 1; 4-hydroxy-2-oxopentanoate aldolase 1

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MNLQGKKITVHDMTLRDGMHPKRHMMTLEQMKSIACGLDAAGVPLIEVTHGDGLGGSSVNYGFPAHSDEEYLGAVIPLMK
QAKVSALLLPGIGTVDHLKMAKELGVHTIRVATHCTEADVSEQHIALARKLEMDTVGFLMMAHMNSPEGLVGQAKLMESY
GANCVYITDSAGYMLPDDVRARLGAVREALKPETELGFHGHHNLAMGIANSIAAVECGATRIDAASAGLGAGAGNTPMEV
LVAVCDRMGIQTGVDVWAIQDVAEDLVVPIMDFPIRIDRDSLTLGYAGVYGSFLLFAKRAERKYGVPAREILVELGRRGM
VGGQEDMIEDTAITLAKARASKAQKVAA

Sequences:

>Translated_348_residues
MNLQGKKITVHDMTLRDGMHPKRHMMTLEQMKSIACGLDAAGVPLIEVTHGDGLGGSSVNYGFPAHSDEEYLGAVIPLMK
QAKVSALLLPGIGTVDHLKMAKELGVHTIRVATHCTEADVSEQHIALARKLEMDTVGFLMMAHMNSPEGLVGQAKLMESY
GANCVYITDSAGYMLPDDVRARLGAVREALKPETELGFHGHHNLAMGIANSIAAVECGATRIDAASAGLGAGAGNTPMEV
LVAVCDRMGIQTGVDVWAIQDVAEDLVVPIMDFPIRIDRDSLTLGYAGVYGSFLLFAKRAERKYGVPAREILVELGRRGM
VGGQEDMIEDTAITLAKARASKAQKVAA
>Mature_348_residues
MNLQGKKITVHDMTLRDGMHPKRHMMTLEQMKSIACGLDAAGVPLIEVTHGDGLGGSSVNYGFPAHSDEEYLGAVIPLMK
QAKVSALLLPGIGTVDHLKMAKELGVHTIRVATHCTEADVSEQHIALARKLEMDTVGFLMMAHMNSPEGLVGQAKLMESY
GANCVYITDSAGYMLPDDVRARLGAVREALKPETELGFHGHHNLAMGIANSIAAVECGATRIDAASAGLGAGAGNTPMEV
LVAVCDRMGIQTGVDVWAIQDVAEDLVVPIMDFPIRIDRDSLTLGYAGVYGSFLLFAKRAERKYGVPAREILVELGRRGM
VGGQEDMIEDTAITLAKARASKAQKVAA

Specific function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-hydroxy-2-oxovalerate aldolase family

Homologues:

Organism=Escherichia coli, GI1786548, Length=333, Percent_Identity=58.8588588588589, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1786261, Length=297, Percent_Identity=25.9259259259259, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HOA1_RALME (Q1LNS4)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_583471.1
- ProteinModelPortal:   Q1LNS4
- SMR:   Q1LNS4
- STRING:   Q1LNS4
- GeneID:   4038122
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_1319
- eggNOG:   COG0119
- HOGENOM:   HBG300122
- OMA:   MMAHMAS
- ProtClustDB:   PRK08195
- BioCyc:   RMET266264:RMET_1319-MONOMER
- HAMAP:   MF_01656
- InterPro:   IPR017629
- InterPro:   IPR013785
- InterPro:   IPR012425
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- ProDom:   PD005364
- TIGRFAMs:   TIGR03217

Pfam domain/function: PF07836 DmpG_comm; PF00682 HMGL-like

EC number: =4.1.3.39

Molecular weight: Translated: 37132; Mature: 37132

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS50991 PYR_CT; PS00599 AA_TRANSFER_CLASS_2

Important sites: BINDING 170-170 BINDING 199-199 BINDING 290-290

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLQGKKITVHDMTLRDGMHPKRHMMTLEQMKSIACGLDAAGVPLIEVTHGDGLGGSSVN
CCCCCCEEEEEEEHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCC
YGFPAHSDEEYLGAVIPLMKQAKVSALLLPGIGTVDHLKMAKELGVHTIRVATHCTEADV
CCCCCCCCHHHHHHHHHHHHHHHHCEEEECCCCCHHHHHHHHHCCCEEEEEEHHCCCCCC
SEQHIALARKLEMDTVGFLMMAHMNSPEGLVGQAKLMESYGANCVYITDSAGYMLPDDVR
CHHHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHH
ARLGAVREALKPETELGFHGHHNLAMGIANSIAAVECGATRIDAASAGLGAGAGNTPMEV
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHEEECCCCEECHHHCCCCCCCCCCHHHH
LVAVCDRMGIQTGVDVWAIQDVAEDLVVPIMDFPIRIDRDSLTLGYAGVYGSFLLFAKRA
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEHHHHHHHHHHHHHHH
ERKYGVPAREILVELGRRGMVGGQEDMIEDTAITLAKARASKAQKVAA
HHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNLQGKKITVHDMTLRDGMHPKRHMMTLEQMKSIACGLDAAGVPLIEVTHGDGLGGSSVN
CCCCCCEEEEEEEHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCC
YGFPAHSDEEYLGAVIPLMKQAKVSALLLPGIGTVDHLKMAKELGVHTIRVATHCTEADV
CCCCCCCCHHHHHHHHHHHHHHHHCEEEECCCCCHHHHHHHHHCCCEEEEEEHHCCCCCC
SEQHIALARKLEMDTVGFLMMAHMNSPEGLVGQAKLMESYGANCVYITDSAGYMLPDDVR
CHHHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHH
ARLGAVREALKPETELGFHGHHNLAMGIANSIAAVECGATRIDAASAGLGAGAGNTPMEV
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHEEECCCCEECHHHCCCCCCCCCCHHHH
LVAVCDRMGIQTGVDVWAIQDVAEDLVVPIMDFPIRIDRDSLTLGYAGVYGSFLLFAKRA
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEHHHHHHHHHHHHHHH
ERKYGVPAREILVELGRRGMVGGQEDMIEDTAITLAKARASKAQKVAA
HHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA