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The map label for this gene is aat

Identifier: 94310161

GI number: 94310161

Start: 1334905

End: 1335654

Strand: Reverse

Name: aat

Synonym: Rmet_1216

Alternate gene names: 94310161

Gene position: 1335654-1334905 (Counterclockwise)

Preceding gene: 94310162

Following gene: 335055518

Centisome position: 34.0

GC content: 63.6

Gene sequence:

>750_bases
ATGATCACCTGGCTCGACCCGCAGGACCCGTTTCCACCGGTCGAGCGCGCGCTCGGACCAGCAAGTGACGCGCCGGGACT
CCTTGCGGCCAGCCGCGATCTTTCACCGCAGCGCCTGCTTCTAGCCTATCGACAAGGCATCTTTCCCTGGTACTCGGAAG
GCCAGCCCGTTCTATGGTGGAGCACCGACCCGCGTATGGTGCTGGCCCCGCATCGGCTGAAGATTTCGGTGTCCCTGCGG
AAGACCCTGCGTCGCATCCTGCGGGACCCCGACTGGGAAATCCGCGTCGATGACGATTTCGTCGCCGTGATGCAGGCTTG
CGCAATGACCCCACGCGATGGCCAGCTCGGCACTTGGATCACGGACGACATCGTCGCAGCGTACGGCAGCCTGCATCGCC
TCGGACTCGCGCACTCTGTCGAGACCTGGTATCGCGGCGAACGCGTTGGCGGCCTCTACGGCGTGGCCCTGGGGCGCATG
TTCTACGGCGAGTCGATGTTCGCCCACCGCACGGATGCGTCGAAGATCGCCCTTGCGGCGCTATGCGGATTCCTCGAACG
CCACGGCGTAACCATGATCGACTGCCAGCAGGAGACCGATCACCTCGCCTCGCTCGGCGCCGAACCAATCCCTCGCGAGC
AGTTCATTGCACACGTTCGCCAGACGGCTGCCGAAGCCAATATCTCTCCCTGGCGTTTTGACAAATCTGAACTGACGCGC
TGGACATCACAGGCAAGCACCGAACTCTGA

Upstream 100 bases:

>100_bases
CGATGCGTCCGCTGACCAGCCGCGCCACGGTCACGCCGTAACAGGCGACGTCCAACCCGCAGGTTTCCCGCAATTCCAGC
CGCAACTCCAGCCTCAGAGC

Downstream 100 bases:

>100_bases
TTGCACGGATCATCGGGGACAAGTGTCGGCGTCACGCGCCCACACTGGCATCAGCGCTCCCTCGACGCGTAACCTGGCGA
TTGTGCACGCTGCAGCCCAC

Product: leucyl/phenylalanyl-tRNA--protein transferase

Products: NA

Alternate protein names: L/F-transferase; Leucyltransferase; Phenyalanyltransferase

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MITWLDPQDPFPPVERALGPASDAPGLLAASRDLSPQRLLLAYRQGIFPWYSEGQPVLWWSTDPRMVLAPHRLKISVSLR
KTLRRILRDPDWEIRVDDDFVAVMQACAMTPRDGQLGTWITDDIVAAYGSLHRLGLAHSVETWYRGERVGGLYGVALGRM
FYGESMFAHRTDASKIALAALCGFLERHGVTMIDCQQETDHLASLGAEPIPREQFIAHVRQTAAEANISPWRFDKSELTR
WTSQASTEL

Sequences:

>Translated_249_residues
MITWLDPQDPFPPVERALGPASDAPGLLAASRDLSPQRLLLAYRQGIFPWYSEGQPVLWWSTDPRMVLAPHRLKISVSLR
KTLRRILRDPDWEIRVDDDFVAVMQACAMTPRDGQLGTWITDDIVAAYGSLHRLGLAHSVETWYRGERVGGLYGVALGRM
FYGESMFAHRTDASKIALAALCGFLERHGVTMIDCQQETDHLASLGAEPIPREQFIAHVRQTAAEANISPWRFDKSELTR
WTSQASTEL
>Mature_249_residues
MITWLDPQDPFPPVERALGPASDAPGLLAASRDLSPQRLLLAYRQGIFPWYSEGQPVLWWSTDPRMVLAPHRLKISVSLR
KTLRRILRDPDWEIRVDDDFVAVMQACAMTPRDGQLGTWITDDIVAAYGSLHRLGLAHSVETWYRGERVGGLYGVALGRM
FYGESMFAHRTDASKIALAALCGFLERHGVTMIDCQQETDHLASLGAEPIPREQFIAHVRQTAAEANISPWRFDKSELTR
WTSQASTEL

Specific function: Functions in the N-end rule pathway of protein degradation where it conjugates Leu, Phe and, less efficiently, Met from aminoacyl-tRNAs to the N-termini of proteins containing an N-terminal arginine or lysine

COG id: COG2360

COG function: function code O; Leu/Phe-tRNA-protein transferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L/F-transferase family

Homologues:

Organism=Escherichia coli, GI1787111, Length=211, Percent_Identity=47.39336492891, Blast_Score=185, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LFTR_RALME (Q1LP24)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_583371.1
- ProteinModelPortal:   Q1LP24
- SMR:   Q1LP24
- STRING:   Q1LP24
- GeneID:   4038018
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_1216
- eggNOG:   COG2360
- HOGENOM:   HBG485363
- OMA:   GEPILWW
- PhylomeDB:   Q1LP24
- ProtClustDB:   PRK00301
- BioCyc:   RMET266264:RMET_1216-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00688
- InterPro:   IPR016181
- InterPro:   IPR004616
- TIGRFAMs:   TIGR00667

Pfam domain/function: PF03588 Leu_Phe_trans; SSF55729 Acyl_CoA_acyltransferase

EC number: =2.3.2.6

Molecular weight: Translated: 28028; Mature: 28028

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITWLDPQDPFPPVERALGPASDAPGLLAASRDLSPQRLLLAYRQGIFPWYSEGQPVLWW
CCCCCCCCCCCCHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCEEEEE
STDPRMVLAPHRLKISVSLRKTLRRILRDPDWEIRVDDDFVAVMQACAMTPRDGQLGTWI
CCCCCEEECCCEEEEEHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHCCCCCCCCCCCCH
TDDIVAAYGSLHRLGLAHSVETWYRGERVGGLYGVALGRMFYGESMFAHRTDASKIALAA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHH
LCGFLERHGVTMIDCQQETDHLASLGAEPIPREQFIAHVRQTAAEANISPWRFDKSELTR
HHHHHHHCCCEEEECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
WTSQASTEL
HHHHHCCCC
>Mature Secondary Structure
MITWLDPQDPFPPVERALGPASDAPGLLAASRDLSPQRLLLAYRQGIFPWYSEGQPVLWW
CCCCCCCCCCCCHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCEEEEE
STDPRMVLAPHRLKISVSLRKTLRRILRDPDWEIRVDDDFVAVMQACAMTPRDGQLGTWI
CCCCCEEECCCEEEEEHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHCCCCCCCCCCCCH
TDDIVAAYGSLHRLGLAHSVETWYRGERVGGLYGVALGRMFYGESMFAHRTDASKIALAA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHH
LCGFLERHGVTMIDCQQETDHLASLGAEPIPREQFIAHVRQTAAEANISPWRFDKSELTR
HHHHHHHCCCEEEECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
WTSQASTEL
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA