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The map label for this gene is aceF [H]

Identifier: 94310142

GI number: 94310142

Start: 1315368

End: 1317032

Strand: Direct

Name: aceF [H]

Synonym: Rmet_1197

Alternate gene names: 94310142

Gene position: 1315368-1317032 (Clockwise)

Preceding gene: 94310141

Following gene: 94310143

Centisome position: 33.49

GC content: 66.55

Gene sequence:

>1665_bases
ATGAGTCAAGCGATTGAAATCAAGGTGCCGGATATCGGCGACTACGACGCCGTTCCCGTCATCGAAGTGCACGTGAAGCC
GGGCGACACGATCAACGCGGAAGACGCGCTGGTGACGCTGGAATCCGACAAGGCCACCATGGACGTGCCGTCGCCGCAGG
CCGGCACGGTCAAGGAAGTCCGGATCAAGGTGGGCGATAGCGTTTCCGAAGGCTCCGTGCTGGTGATGCTCGAGCCCGCA
GGCGCCGCTGCCGCCGCTCCGGCTCCCGCAGCCGCTGCTGCTCCGGCAGCTCCGGCCCCTGCCGCCGCGGCACCCGCGCC
GGCGGCTCCCGCTGCCCCGGCTCCGGCCGCTGCCCCTGCTGGTGGTGGCACGGTCGAGGTCAAGGTGCCCGATATCGGCG
ACTACGACGCCGTGCCGGTCATCGAAATCCACGTCAAGGTGGGTGACCAGATCAACGCCGAAGACGCGTTGGTGACGCTG
GAGTCCGACAAGGCCACCATGGATGTGCCGTCGCCGCAGGCCGGCACGGTCAAGGAAATCAAGGTCAAGGTCGGTGATAA
CGTCGCCCAAGGCACGCTGATCCTGATCCTGGAAGCTGCTGGCGGCGCTGCCGCAGCAGCTCCGGCCCCGGCAGCCGCCC
CGGCGCCTGCCGCAGCCGCTCCCGCGCCCGCACCGGCGGCCGCGGCGCCGGCACCTGTTGCCGCGCCGGCCGTCGCGCCA
GCGGTGCAGGGTACGACCGGCAAGGCCGCTCACGCCAGCCCGACCGTGCGCAAGTTCGCGCGCGAGCTGGGTGTCGACGT
GTCGCGTGTGCCGGGCACCGCTCCCAAGGGCCGCATCACGCAGGAAGACGTGCAGAACTACGTCAAGAGCGTCATGAGCG
GCCAGACTGCCACGCCGTCCGCACCGGCTGCCGCCGCCGGCACCGGCGTGGGCCTGGACCTGCTGCCGTGGCCGAAGGTG
GACTTCACGCGCTTTGGCGAAGTGGAGTCGAAGCCGCTGTCGCGCATCAAGAAGATCTCTGGCGCCAACCTGCATCGCAA
CTGGGTCATGATCCCTCACGTCACGAACTGTGACGAAGCGGACATCACCGAGCTCGAGGCATTCCGCGTGCAGCTCAACA
AGGAAAACGAGAAGGCTGGCATCAAGGTGACGATGCTTGCGTTCATGATCAAGGCCACCGTTGCGGCGCTCAAGAAGTTC
CCGAACTTCAACGCCTCGCTGGACGGCGACAACCTGGTGCTGAAGAAGTACTTCAACATCGGTTTCGCGGCCGACACCCC
GAACGGTCTGGTCGTGCCGGTGATCAAGGACGCCGACAAGAAGGGCGTGCTCGAGATCAGCCAGGAAATGAGCGATCTGG
CCAAGCTGGCGCGCGACGGCAAGCTGAAGCCTGACCAGATGCAAGGCGGCTGCTTCTCGATCTCGTCGCTCGGCGGCCTC
GGTGGCACGTACTTCACGCCGATCATCAATGCGCCGGAAGTGGCCATCATGGGCGTGTGCAAGTCGTATATGAAGCCGGT
GTGGGACGGCAAGCAGTTCGCCCCGCGCCTGACGCTGCCGCTGTCGCTGTCGTGGGATCACCGCGTGATCGACGGTGCCG
AGGCCGCACGCTTCAACACGTACTTCGCGGCGCTGCTGGCGGATTTCCGCCGGATTCTGCTGTAA

Upstream 100 bases:

>100_bases
GCAGCGTGACAGATGCGTGTGGCGAGAGTAACCTCGCCGATTGCGTTTGTCGCGTTTGTCGTATCTGGCCAGAGTGCCGG
CTGCCCAGGAGAGACACTGA

Downstream 100 bases:

>100_bases
GCATCGGCAACGCCCGCCAGCGAACCTGGCGGGCGCGTCTGCATTGCATTCAAGGGGCGAGCCATGACTACCTGCGTTGT
CGTCAGGAAAGGTGACGAGG

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MSQAIEIKVPDIGDYDAVPVIEVHVKPGDTINAEDALVTLESDKATMDVPSPQAGTVKEVRIKVGDSVSEGSVLVMLEPA
GAAAAAPAPAAAAAPAAPAPAAAAPAPAAPAAPAPAAAPAGGGTVEVKVPDIGDYDAVPVIEIHVKVGDQINAEDALVTL
ESDKATMDVPSPQAGTVKEIKVKVGDNVAQGTLILILEAAGGAAAAAPAPAAAPAPAAAAPAPAPAAAAPAPVAAPAVAP
AVQGTTGKAAHASPTVRKFARELGVDVSRVPGTAPKGRITQEDVQNYVKSVMSGQTATPSAPAAAAGTGVGLDLLPWPKV
DFTRFGEVESKPLSRIKKISGANLHRNWVMIPHVTNCDEADITELEAFRVQLNKENEKAGIKVTMLAFMIKATVAALKKF
PNFNASLDGDNLVLKKYFNIGFAADTPNGLVVPVIKDADKKGVLEISQEMSDLAKLARDGKLKPDQMQGGCFSISSLGGL
GGTYFTPIINAPEVAIMGVCKSYMKPVWDGKQFAPRLTLPLSLSWDHRVIDGAEAARFNTYFAALLADFRRILL

Sequences:

>Translated_554_residues
MSQAIEIKVPDIGDYDAVPVIEVHVKPGDTINAEDALVTLESDKATMDVPSPQAGTVKEVRIKVGDSVSEGSVLVMLEPA
GAAAAAPAPAAAAAPAAPAPAAAAPAPAAPAAPAPAAAPAGGGTVEVKVPDIGDYDAVPVIEIHVKVGDQINAEDALVTL
ESDKATMDVPSPQAGTVKEIKVKVGDNVAQGTLILILEAAGGAAAAAPAPAAAPAPAAAAPAPAPAAAAPAPVAAPAVAP
AVQGTTGKAAHASPTVRKFARELGVDVSRVPGTAPKGRITQEDVQNYVKSVMSGQTATPSAPAAAAGTGVGLDLLPWPKV
DFTRFGEVESKPLSRIKKISGANLHRNWVMIPHVTNCDEADITELEAFRVQLNKENEKAGIKVTMLAFMIKATVAALKKF
PNFNASLDGDNLVLKKYFNIGFAADTPNGLVVPVIKDADKKGVLEISQEMSDLAKLARDGKLKPDQMQGGCFSISSLGGL
GGTYFTPIINAPEVAIMGVCKSYMKPVWDGKQFAPRLTLPLSLSWDHRVIDGAEAARFNTYFAALLADFRRILL
>Mature_553_residues
SQAIEIKVPDIGDYDAVPVIEVHVKPGDTINAEDALVTLESDKATMDVPSPQAGTVKEVRIKVGDSVSEGSVLVMLEPAG
AAAAAPAPAAAAAPAAPAPAAAAPAPAAPAAPAPAAAPAGGGTVEVKVPDIGDYDAVPVIEIHVKVGDQINAEDALVTLE
SDKATMDVPSPQAGTVKEIKVKVGDNVAQGTLILILEAAGGAAAAAPAPAAAPAPAAAAPAPAPAAAAPAPVAAPAVAPA
VQGTTGKAAHASPTVRKFARELGVDVSRVPGTAPKGRITQEDVQNYVKSVMSGQTATPSAPAAAAGTGVGLDLLPWPKVD
FTRFGEVESKPLSRIKKISGANLHRNWVMIPHVTNCDEADITELEAFRVQLNKENEKAGIKVTMLAFMIKATVAALKKFP
NFNASLDGDNLVLKKYFNIGFAADTPNGLVVPVIKDADKKGVLEISQEMSDLAKLARDGKLKPDQMQGGCFSISSLGGLG
GTYFTPIINAPEVAIMGVCKSYMKPVWDGKQFAPRLTLPLSLSWDHRVIDGAEAARFNTYFAALLADFRRILL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=462, Percent_Identity=29.6536796536797, Blast_Score=176, Evalue=7e-44,
Organism=Homo sapiens, GI31711992, Length=423, Percent_Identity=32.8605200945626, Blast_Score=161, Evalue=2e-39,
Organism=Homo sapiens, GI203098816, Length=499, Percent_Identity=27.6553106212425, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI203098753, Length=436, Percent_Identity=28.6697247706422, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI19923748, Length=233, Percent_Identity=32.618025751073, Blast_Score=119, Evalue=1e-26,
Organism=Homo sapiens, GI260898739, Length=162, Percent_Identity=37.037037037037, Blast_Score=98, Evalue=3e-20,
Organism=Escherichia coli, GI1786305, Length=554, Percent_Identity=54.5126353790614, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI1786946, Length=436, Percent_Identity=29.5871559633028, Blast_Score=173, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=434, Percent_Identity=30.8755760368664, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17560088, Length=438, Percent_Identity=31.5068493150685, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI25146366, Length=202, Percent_Identity=34.1584158415842, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17538894, Length=314, Percent_Identity=30.2547770700637, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320352, Length=415, Percent_Identity=30.3614457831325, Blast_Score=165, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6324258, Length=435, Percent_Identity=28.5057471264368, Blast_Score=135, Evalue=1e-32,
Organism=Drosophila melanogaster, GI18859875, Length=446, Percent_Identity=30.7174887892377, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24582497, Length=225, Percent_Identity=35.1111111111111, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI20129315, Length=225, Percent_Identity=35.1111111111111, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24645909, Length=186, Percent_Identity=36.0215053763441, Blast_Score=117, Evalue=3e-26,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 56869; Mature: 56737

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQAIEIKVPDIGDYDAVPVIEVHVKPGDTINAEDALVTLESDKATMDVPSPQAGTVKEV
CCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCEECCCCCCCCCEEEE
RIKVGDSVSEGSVLVMLEPAGAAAAAPAPAAAAAPAAPAPAAAAPAPAAPAAPAPAAAPA
EEEECCCCCCCEEEEEECCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GGGTVEVKVPDIGDYDAVPVIEIHVKVGDQINAEDALVTLESDKATMDVPSPQAGTVKEI
CCCEEEEECCCCCCCCCCEEEEEEEEECCCCCCCCEEEEEECCCCEECCCCCCCCCEEEE
KVKVGDNVAQGTLILILEAAGGAAAAAPAPAAAPAPAAAAPAPAPAAAAPAPVAAPAVAP
EEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
AVQGTTGKAAHASPTVRKFARELGVDVSRVPGTAPKGRITQEDVQNYVKSVMSGQTATPS
CCCCCCCCCCCCCHHHHHHHHHHCCCEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
APAAAAGTGVGLDLLPWPKVDFTRFGEVESKPLSRIKKISGANLHRNWVMIPHVTNCDEA
CCCCCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHCCCCEECCEEEECCCCCCCCC
DITELEAFRVQLNKENEKAGIKVTMLAFMIKATVAALKKFPNFNASLDGDNLVLKKYFNI
CHHHHEEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCC
GFAADTPNGLVVPVIKDADKKGVLEISQEMSDLAKLARDGKLKPDQMQGGCFSISSLGGL
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCEEEEHHCCCC
GGTYFTPIINAPEVAIMGVCKSYMKPVWDGKQFAPRLTLPLSLSWDHRVIDGAEAARFNT
CCHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCEECCCHHHHHHH
YFAALLADFRRILL
HHHHHHHHHHHHHC
>Mature Secondary Structure 
SQAIEIKVPDIGDYDAVPVIEVHVKPGDTINAEDALVTLESDKATMDVPSPQAGTVKEV
CCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCEECCCCCCCCCEEEE
RIKVGDSVSEGSVLVMLEPAGAAAAAPAPAAAAAPAAPAPAAAAPAPAAPAAPAPAAAPA
EEEECCCCCCCEEEEEECCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GGGTVEVKVPDIGDYDAVPVIEIHVKVGDQINAEDALVTLESDKATMDVPSPQAGTVKEI
CCCEEEEECCCCCCCCCCEEEEEEEEECCCCCCCCEEEEEECCCCEECCCCCCCCCEEEE
KVKVGDNVAQGTLILILEAAGGAAAAAPAPAAAPAPAAAAPAPAPAAAAPAPVAAPAVAP
EEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
AVQGTTGKAAHASPTVRKFARELGVDVSRVPGTAPKGRITQEDVQNYVKSVMSGQTATPS
CCCCCCCCCCCCCHHHHHHHHHHCCCEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
APAAAAGTGVGLDLLPWPKVDFTRFGEVESKPLSRIKKISGANLHRNWVMIPHVTNCDEA
CCCCCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHCCCCEECCEEEECCCCCCCCC
DITELEAFRVQLNKENEKAGIKVTMLAFMIKATVAALKKFPNFNASLDGDNLVLKKYFNI
CHHHHEEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCC
GFAADTPNGLVVPVIKDADKKGVLEISQEMSDLAKLARDGKLKPDQMQGGCFSISSLGGL
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCEEEEHHCCCC
GGTYFTPIINAPEVAIMGVCKSYMKPVWDGKQFAPRLTLPLSLSWDHRVIDGAEAARFNT
CCHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCEECCCHHHHHHH
YFAALLADFRRILL
HHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8021225 [H]