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The map label for this gene is trpI [H]

Identifier: 94310120

GI number: 94310120

Start: 1286349

End: 1287248

Strand: Reverse

Name: trpI [H]

Synonym: Rmet_1175

Alternate gene names: 94310120

Gene position: 1287248-1286349 (Counterclockwise)

Preceding gene: 94310122

Following gene: 94310115

Centisome position: 32.77

GC content: 67.22

Gene sequence:

>900_bases
GTGCGAACCCTACCCCCAATGTCAACCTTGCGCGCGTTCGAGGCCACGGCCCGTACGGGCTCCGTGACCCGCGCCGCCGA
AGAGCTATTTCGCACGCACGGTGCAGTCAGCCGGCAGTTGCGCCAACTGCAGGAACATGCGGGCGTGGAGCTATTCGAGC
GCGAGGGGACCGGCCTGCGCCTGAACGAGCACGGCCAGGCGCTCTATGAGGTGGTACGCACGGCGCTGGACCAACTCGAA
CAAGGTTACGGTCGTCTGCTCGACCAAGCGCGCGGGCCGACGCTGCACGTGGCATGTAGCGCGACCTTCGCAATGCGCTG
GCTGGTACCCGAATTGGTCGATTTCTATCGGCTGCGGCCCGATATCCGTATCCGGCTCTCGATGACATCGGCGCGCGAGA
TCCGCACCGAAGGCGCAGACGTGGTAATTGCCTGGGACCTGTCGTCGTACCCCGCCGCCGAACGGCAACGCGCGATCCCG
TTGGCGCCGGTCGCCTTCGGCCCGGTCTGCGCGCCCGCCTATGCACCGGAAGTGGCCACCGGCGCGCGCATCACGCACGA
TTTCACATCGAGCGCTTGGGACCAGTGGGAAGCGCTGACCGGTCACCACATCGCACCCGGCGCAGGGTTGACGTTTCCGC
ACACGCATCTTTGCATCCAGGCCGCCCTGTCGGGGCTGGGCGTGGCGCTGATCGAACAACGGCTGATCCAGGCGGAACTG
GCAGCAGGCACCCTCGTGGCACCCTTCGGATTCACCGCGTTTGGCGGCGGTCTGATGGCGGTCCCGGCCACGCGAGACGT
GCCGGCCGATGCCGACGTGTTTATCGCGTGGCTGCAGCGTCGGCTATTGCCGCCGGTCGACTCTCGCGCCCCGCGACAGG
CAGGACAAACCGGAGCGTAG

Upstream 100 bases:

>100_bases
GAAAAATGAAGATTCGTCGATCCTAAAAGGCTTTGACCAAGCTGAATATCGCGTTATTTTCCTCAATTCGTCAGCTTTTC
TCACATAGCCGGAGCGTACG

Downstream 100 bases:

>100_bases
TGCCCACCTGCCCCTCCACCAGCCGCGCCTGACTGCCGTTCAACTGGAGCATTCGCTGCACGATCAGCAGACCGAGGCCG
CCGCCCCGGTGCGCGCCGCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: TrpBA operon transcriptional activator [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MRTLPPMSTLRAFEATARTGSVTRAAEELFRTHGAVSRQLRQLQEHAGVELFEREGTGLRLNEHGQALYEVVRTALDQLE
QGYGRLLDQARGPTLHVACSATFAMRWLVPELVDFYRLRPDIRIRLSMTSAREIRTEGADVVIAWDLSSYPAAERQRAIP
LAPVAFGPVCAPAYAPEVATGARITHDFTSSAWDQWEALTGHHIAPGAGLTFPHTHLCIQAALSGLGVALIEQRLIQAEL
AAGTLVAPFGFTAFGGGLMAVPATRDVPADADVFIAWLQRRLLPPVDSRAPRQAGQTGA

Sequences:

>Translated_299_residues
MRTLPPMSTLRAFEATARTGSVTRAAEELFRTHGAVSRQLRQLQEHAGVELFEREGTGLRLNEHGQALYEVVRTALDQLE
QGYGRLLDQARGPTLHVACSATFAMRWLVPELVDFYRLRPDIRIRLSMTSAREIRTEGADVVIAWDLSSYPAAERQRAIP
LAPVAFGPVCAPAYAPEVATGARITHDFTSSAWDQWEALTGHHIAPGAGLTFPHTHLCIQAALSGLGVALIEQRLIQAEL
AAGTLVAPFGFTAFGGGLMAVPATRDVPADADVFIAWLQRRLLPPVDSRAPRQAGQTGA
>Mature_299_residues
MRTLPPMSTLRAFEATARTGSVTRAAEELFRTHGAVSRQLRQLQEHAGVELFEREGTGLRLNEHGQALYEVVRTALDQLE
QGYGRLLDQARGPTLHVACSATFAMRWLVPELVDFYRLRPDIRIRLSMTSAREIRTEGADVVIAWDLSSYPAAERQRAIP
LAPVAFGPVCAPAYAPEVATGARITHDFTSSAWDQWEALTGHHIAPGAGLTFPHTHLCIQAALSGLGVALIEQRLIQAEL
AAGTLVAPFGFTAFGGGLMAVPATRDVPADADVFIAWLQRRLLPPVDSRAPRQAGQTGA

Specific function: Activates the expression of the trpBA genes encoding the two tryptophan synthase subunits. In the absence of the inducer (indoleglycerol phosphate), trpI binds upstream of the trpAB operon, overlapping its own promoter region [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=259, Percent_Identity=33.5907335907336, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI1786448, Length=289, Percent_Identity=31.8339100346021, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788706, Length=259, Percent_Identity=31.2741312741313, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI157672245, Length=169, Percent_Identity=34.9112426035503, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788481, Length=261, Percent_Identity=26.8199233716475, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787128, Length=249, Percent_Identity=24.0963855421687, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32461; Mature: 32461

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTLPPMSTLRAFEATARTGSVTRAAEELFRTHGAVSRQLRQLQEHAGVELFEREGTGLR
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCE
LNEHGQALYEVVRTALDQLEQGYGRLLDQARGPTLHVACSATFAMRWLVPELVDFYRLRP
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCC
DIRIRLSMTSAREIRTEGADVVIAWDLSSYPAAERQRAIPLAPVAFGPVCAPAYAPEVAT
CEEEEEEECHHHHHHCCCCCEEEEECCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCC
GARITHDFTSSAWDQWEALTGHHIAPGAGLTFPHTHLCIQAALSGLGVALIEQRLIQAEL
CCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAGTLVAPFGFTAFGGGLMAVPATRDVPADADVFIAWLQRRLLPPVDSRAPRQAGQTGA
HCCCEECCCCHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRTLPPMSTLRAFEATARTGSVTRAAEELFRTHGAVSRQLRQLQEHAGVELFEREGTGLR
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCE
LNEHGQALYEVVRTALDQLEQGYGRLLDQARGPTLHVACSATFAMRWLVPELVDFYRLRP
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCC
DIRIRLSMTSAREIRTEGADVVIAWDLSSYPAAERQRAIPLAPVAFGPVCAPAYAPEVAT
CEEEEEEECHHHHHHCCCCCEEEEECCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCC
GARITHDFTSSAWDQWEALTGHHIAPGAGLTFPHTHLCIQAALSGLGVALIEQRLIQAEL
CCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAGTLVAPFGFTAFGGGLMAVPATRDVPADADVFIAWLQRRLLPPVDSRAPRQAGQTGA
HCCCEECCCCHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8423001 [H]