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The map label for this gene is pheS [H]

Identifier: 94310109

GI number: 94310109

Start: 1273513

End: 1274544

Strand: Direct

Name: pheS [H]

Synonym: Rmet_1164

Alternate gene names: 94310109

Gene position: 1273513-1274544 (Clockwise)

Preceding gene: 94310108

Following gene: 94310110

Centisome position: 32.42

GC content: 63.76

Gene sequence:

>1032_bases
ATGTCCCTGGATCTGGATCAAGTCGTCGCCGACGCCCAGGCTGCTTTCGCCGCCGCCAACGACAACGCCACGCTGGAAAA
CGAAAAGGCCCGCTTTCTGGGTAAGACCGGTGCGCTGACCGAGCTGCTCAAGGGCCTCGGCAAGCTGGACCCGGACGCGC
GCAAGACCGAAGGTGCGCGTATCAATCAGGTCAAGCAGCAGGTGGAAGCCGCGCTGCAGGCCCGCCGCCAGGCGCTGGCC
GATGCGCTGATGAACGCGCGTCTGGCCGCGGAAGCTATCGACGTGACGCTGCCCGGCCGCTCTGTTGCGCGTGGCAGCGT
GCATCCGGTGATGCGTACCTGGGAGCGCGTCGAGCAGATCTTCGGCTCGATCGGTTTCGACGTGGCCGACGGCCCTGAAA
TCGAAACCGACTGGATGAACTTCACCGCGCTGAACAACCCGGACAACCATCCGGCGCGTTCGATGCAGGACACGTTCTAT
GTGGACGGCCGCGACAGCGACGACAAGCTCCTGCTGCTGCGTACCCACACCAGCCCGATGCAGGTGCGTTACGCGCGCAT
GCACGTCGAGAAGTACGCCGGCCGGGCCATGCCGCCGATCAAGGTGATCTGCCCGGGCCGCACGTACCGCGTGGACAGCG
ATGCCACGCACTCGCCGATGTTCAATCAGGTGGAAGGTCTGTGGATTGGCGATAACGTGAGCTTTGCCGACCTGAAGGGC
GTCTACACCGATTTCCTGCGCAAGTTCTTCGAGCGCGACGATATCCAGGTGCGCTTCCGTCCGTCGTACTTCCCGTTCAC
CGAGCCGTCGGCTGAAATCGACATGGCGTTCGGCAACGGCAAGTGGCTGGAAATCTCCGGCTCGGGCCAGGTGCACCCCA
ACGTGCTGCGCAATATGGGCCTCGATCCCGAGCGCTACATCGGCTTTGCGTTCGGCTCCGGCCTCGAGCGCCTGACGATG
CTGCGCTATGGCATCAATGACCTGCGACTGTTCTTCGAAGGCGATGTGCGCTTCCTGCGCCAGTTCGCCTGA

Upstream 100 bases:

>100_bases
GATCGGAGCGATCCGAGACGGCATTCGAAATAGATGCGCCGCACGCGCATTGCCGCGTGCAGTGTCAACCTTTCCACGTA
TTTGTCTACGTATTGCCGCC

Downstream 100 bases:

>100_bases
TATGGCGTACCGCCCGAAGCAATAACGCAGCTCCAACGTCCCAGACAACTCAGAATACCTACCGGATCAGAAGCGCCATG
CAATTCTCGGAATCCTGGCT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MSLDLDQVVADAQAAFAAANDNATLENEKARFLGKTGALTELLKGLGKLDPDARKTEGARINQVKQQVEAALQARRQALA
DALMNARLAAEAIDVTLPGRSVARGSVHPVMRTWERVEQIFGSIGFDVADGPEIETDWMNFTALNNPDNHPARSMQDTFY
VDGRDSDDKLLLLRTHTSPMQVRYARMHVEKYAGRAMPPIKVICPGRTYRVDSDATHSPMFNQVEGLWIGDNVSFADLKG
VYTDFLRKFFERDDIQVRFRPSYFPFTEPSAEIDMAFGNGKWLEISGSGQVHPNVLRNMGLDPERYIGFAFGSGLERLTM
LRYGINDLRLFFEGDVRFLRQFA

Sequences:

>Translated_343_residues
MSLDLDQVVADAQAAFAAANDNATLENEKARFLGKTGALTELLKGLGKLDPDARKTEGARINQVKQQVEAALQARRQALA
DALMNARLAAEAIDVTLPGRSVARGSVHPVMRTWERVEQIFGSIGFDVADGPEIETDWMNFTALNNPDNHPARSMQDTFY
VDGRDSDDKLLLLRTHTSPMQVRYARMHVEKYAGRAMPPIKVICPGRTYRVDSDATHSPMFNQVEGLWIGDNVSFADLKG
VYTDFLRKFFERDDIQVRFRPSYFPFTEPSAEIDMAFGNGKWLEISGSGQVHPNVLRNMGLDPERYIGFAFGSGLERLTM
LRYGINDLRLFFEGDVRFLRQFA
>Mature_342_residues
SLDLDQVVADAQAAFAAANDNATLENEKARFLGKTGALTELLKGLGKLDPDARKTEGARINQVKQQVEAALQARRQALAD
ALMNARLAAEAIDVTLPGRSVARGSVHPVMRTWERVEQIFGSIGFDVADGPEIETDWMNFTALNNPDNHPARSMQDTFYV
DGRDSDDKLLLLRTHTSPMQVRYARMHVEKYAGRAMPPIKVICPGRTYRVDSDATHSPMFNQVEGLWIGDNVSFADLKGV
YTDFLRKFFERDDIQVRFRPSYFPFTEPSAEIDMAFGNGKWLEISGSGQVHPNVLRNMGLDPERYIGFAFGSGLERLTML
RYGINDLRLFFEGDVRFLRQFA

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=264, Percent_Identity=33.7121212121212, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI5729820, Length=294, Percent_Identity=29.9319727891156, Blast_Score=115, Evalue=8e-26,
Organism=Escherichia coli, GI1788007, Length=338, Percent_Identity=55.9171597633136, Blast_Score=382, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17508957, Length=267, Percent_Identity=34.4569288389513, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI32563657, Length=267, Percent_Identity=34.4569288389513, Blast_Score=138, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI32566635, Length=253, Percent_Identity=32.0158102766798, Blast_Score=112, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6321087, Length=364, Percent_Identity=29.1208791208791, Blast_Score=132, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6325304, Length=90, Percent_Identity=44.4444444444444, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI18858079, Length=289, Percent_Identity=31.4878892733564, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI17137424, Length=253, Percent_Identity=28.0632411067194, Blast_Score=88, Evalue=7e-18,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 38517; Mature: 38386

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLDLDQVVADAQAAFAAANDNATLENEKARFLGKTGALTELLKGLGKLDPDARKTEGAR
CCCCHHHHHHHHHHHHEECCCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCHHHCCCCH
INQVKQQVEAALQARRQALADALMNARLAAEAIDVTLPGRSVARGSVHPVMRTWERVEQI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHH
FGSIGFDVADGPEIETDWMNFTALNNPDNHPARSMQDTFYVDGRDSDDKLLLLRTHTSPM
HHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECCCCH
QVRYARMHVEKYAGRAMPPIKVICPGRTYRVDSDATHSPMFNQVEGLWIGDNVSFADLKG
HHHHHHHHHHHHCCCCCCCEEEEECCCEEEECCCCCCCCCHHCCCCEEECCCCCHHHHHH
VYTDFLRKFFERDDIQVRFRPSYFPFTEPSAEIDMAFGNGKWLEISGSGQVHPNVLRNMG
HHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEECCCEEEEECCCCCCCHHHHHHCC
LDPERYIGFAFGSGLERLTMLRYGINDLRLFFEGDVRFLRQFA
CCHHHHEEEHHHCHHHHHHHHHHCCCCEEEEEHHHHHHHHHCC
>Mature Secondary Structure 
SLDLDQVVADAQAAFAAANDNATLENEKARFLGKTGALTELLKGLGKLDPDARKTEGAR
CCCHHHHHHHHHHHHEECCCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCHHHCCCCH
INQVKQQVEAALQARRQALADALMNARLAAEAIDVTLPGRSVARGSVHPVMRTWERVEQI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHH
FGSIGFDVADGPEIETDWMNFTALNNPDNHPARSMQDTFYVDGRDSDDKLLLLRTHTSPM
HHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECCCCH
QVRYARMHVEKYAGRAMPPIKVICPGRTYRVDSDATHSPMFNQVEGLWIGDNVSFADLKG
HHHHHHHHHHHHCCCCCCCEEEEECCCEEEECCCCCCCCCHHCCCCEEECCCCCHHHHHH
VYTDFLRKFFERDDIQVRFRPSYFPFTEPSAEIDMAFGNGKWLEISGSGQVHPNVLRNMG
HHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEECCCEEEEECCCCCCCHHHHHHCC
LDPERYIGFAFGSGLERLTMLRYGINDLRLFFEGDVRFLRQFA
CCHHHHEEEHHHCHHHHHHHHHHCCCCEEEEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA