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The map label for this gene is rpoI [H]

Identifier: 94310065

GI number: 94310065

Start: 1223515

End: 1224015

Strand: Reverse

Name: rpoI [H]

Synonym: Rmet_1120

Alternate gene names: 94310065

Gene position: 1224015-1223515 (Counterclockwise)

Preceding gene: 94310066

Following gene: 94310064

Centisome position: 31.16

GC content: 63.87

Gene sequence:

>501_bases
ATGGCCTCGACGCGCCTGCGTGCCCTCGGTCTGTACGTGCACTACCAGGAGCTGGTCGGTACCTGGACGCGCAGGCTCAG
CAATCGGCACGAGGCCGAGGACCTGTCGCAAGAAGCCATGCTGCGCGTGCTGGAAGCCCGGGTAACGCCCGAGCAGCCAC
GCGCCTATCTGCACCAGACCGCCCGCAATATCGCGATCGACACCTTCCGCAAACGAGACGGGCACGAGCACCTGCCGCTT
GACAGCATCGAGTCGATTCACGCACCCTCCGGAAATCCCGAGGCGGACCTGCATGCCGTGCAGGTGGTCGCGGGGCTGGA
GCGCGCGCTGGCCGAACTGCCCTTGAAGTGCCGCCAGGTCTTTATCTGGCAACGGCTGGACGGGTGCTCCCAGCAGGAAA
TCGCCGAGCGGCTGAATATCTCGAAGAACATGGTCGAGAAATACATGATTCGCGCGATGCGACACCTGCGCGACCAACTG
GGCCAACTCTCACCTCACTAG

Upstream 100 bases:

>100_bases
CAGCCGCGCATTGCATGTCGCGGTTGACGCCTTCATCACCTACAAATGATAATAGGTCGCATTTGCATCAAAACGCTTAC
TCTTAGCTGACACTCCATCC

Downstream 100 bases:

>100_bases
AACAATCTTGTCGATGCCTGCCCCATATCCGTCTGGCGGCGTCTGCTCCCCCCCAAGCAGCCCATGCAGCGGTCTTGCGC
CCCGGTCGAGAATCCGATGA

Product: RNA polymerase sigma I (sigma 19) factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MASTRLRALGLYVHYQELVGTWTRRLSNRHEAEDLSQEAMLRVLEARVTPEQPRAYLHQTARNIAIDTFRKRDGHEHLPL
DSIESIHAPSGNPEADLHAVQVVAGLERALAELPLKCRQVFIWQRLDGCSQQEIAERLNISKNMVEKYMIRAMRHLRDQL
GQLSPH

Sequences:

>Translated_166_residues
MASTRLRALGLYVHYQELVGTWTRRLSNRHEAEDLSQEAMLRVLEARVTPEQPRAYLHQTARNIAIDTFRKRDGHEHLPL
DSIESIHAPSGNPEADLHAVQVVAGLERALAELPLKCRQVFIWQRLDGCSQQEIAERLNISKNMVEKYMIRAMRHLRDQL
GQLSPH
>Mature_165_residues
ASTRLRALGLYVHYQELVGTWTRRLSNRHEAEDLSQEAMLRVLEARVTPEQPRAYLHQTARNIAIDTFRKRDGHEHLPLD
SIESIHAPSGNPEADLHAVQVVAGLERALAELPLKCRQVFIWQRLDGCSQQEIAERLNISKNMVEKYMIRAMRHLRDQLG
QLSPH

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates the fec genes for iron dicitrate transport (Probable) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790746, Length=153, Percent_Identity=31.3725490196078, Blast_Score=70, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 19132; Mature: 19001

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASTRLRALGLYVHYQELVGTWTRRLSNRHEAEDLSQEAMLRVLEARVTPEQPRAYLHQT
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
ARNIAIDTFRKRDGHEHLPLDSIESIHAPSGNPEADLHAVQVVAGLERALAELPLKCRQV
HHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHH
FIWQRLDGCSQQEIAERLNISKNMVEKYMIRAMRHLRDQLGQLSPH
HHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ASTRLRALGLYVHYQELVGTWTRRLSNRHEAEDLSQEAMLRVLEARVTPEQPRAYLHQT
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
ARNIAIDTFRKRDGHEHLPLDSIESIHAPSGNPEADLHAVQVVAGLERALAELPLKCRQV
HHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHH
FIWQRLDGCSQQEIAERLNISKNMVEKYMIRAMRHLRDQLGQLSPH
HHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2254251; 7610040; 9278503; 8052622 [H]