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The map label for this gene is hpcH [H]

Identifier: 94310056

GI number: 94310056

Start: 1210197

End: 1210952

Strand: Reverse

Name: hpcH [H]

Synonym: Rmet_1111

Alternate gene names: 94310056

Gene position: 1210952-1210197 (Counterclockwise)

Preceding gene: 94310057

Following gene: 94310055

Centisome position: 30.83

GC content: 68.92

Gene sequence:

>756_bases
TTGAAGCAGCGCTTGCTCACACCAGGCAGCGAACCCATCGTCGGCCTGTTCTGCTCGACACCGGCACCGCTCACGGTGGA
ACTGATCGCCGCCGCTGGCTACGACTTCGCGGTGATCGATCTGGAGCACACGCTGATCGATGGCGCATTGCTTGGCGCGA
TGCTGCTGGCGGCGCGTGCCAGTGGCATCGCTCCGCTGGTGCGCGTGGCAGCGTTGCATCAGGTGGCACCGGCGCTGGAC
GCCGGCGCGCAAGGGATTGTCTTCCCGCGCATTACCGGTGCAGCGCGGGCAAGCGAAGCGGTGGCATACTGCCACCACAC
CTGCGGCATCCCGGCAGGCAAGCGCGGCCTCAATGCCACCTGGCACAGCGGCTACGGCCGGGACGATCTGTGCGAAGCCG
CCGAGGACGCCGCACGGCACACGCTCGTTGTGGCGATGATCGAAGACGCCGCGGGCTTGCGCAACGCCGATGACATCGCG
GCGCAACGGGGCGTCGACGTGCTGCTCGAAGGTGCGGCCGATCTCTCCCAGTCGCTGGGCGTGCCGTGGCAAACCCGCCA
TCCGCACGTGCGCGACGCCGTGGATACGATCGCCGCCGCCGCGTTGCGTCATGACAAACACTTCTGCGCCCTGCCCCGTA
CACCCGCTGACGCCGCCGCATGGCGTGCACGGGGCACCCGGATGATGGTGCTTGGCGACGATCGCGGCATCGCCCGCCGT
GCGATGGCCGCCCACCGGCAGGCGTGCATTGCCTAG

Upstream 100 bases:

>100_bases
TCGCAAGTGCGCCCACATCAGCGCTCACCGGCTCGCCCACAGCCGCGCCCGCAACCAGGCTTACGACAGCCCACGCACCG
ACGGAGGCATCGTGCAATCG

Downstream 100 bases:

>100_bases
CAGCAGCAGGACATAAGCTGCCTCGAACAGGAACAACCATGGATCTGACACACGTTACCCGCTACATCGCCGGGCGGGAT
ACTCGCGAGCCGGTCTGCGC

Product: HpcH/HpaI aldolase

Products: NA

Alternate protein names: KDGluc aldolase; KDGlucA; 2-dehydro-3-deoxy-D-glucarate aldolase; 2-keto-3-deoxy-D-glucarate aldolase; 5-dehydro-4-deoxy-D-glucarate aldolase; Alpha-keto-beta-deoxy-D-glucarate aldolase [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MKQRLLTPGSEPIVGLFCSTPAPLTVELIAAAGYDFAVIDLEHTLIDGALLGAMLLAARASGIAPLVRVAALHQVAPALD
AGAQGIVFPRITGAARASEAVAYCHHTCGIPAGKRGLNATWHSGYGRDDLCEAAEDAARHTLVVAMIEDAAGLRNADDIA
AQRGVDVLLEGAADLSQSLGVPWQTRHPHVRDAVDTIAAAALRHDKHFCALPRTPADAAAWRARGTRMMVLGDDRGIARR
AMAAHRQACIA

Sequences:

>Translated_251_residues
MKQRLLTPGSEPIVGLFCSTPAPLTVELIAAAGYDFAVIDLEHTLIDGALLGAMLLAARASGIAPLVRVAALHQVAPALD
AGAQGIVFPRITGAARASEAVAYCHHTCGIPAGKRGLNATWHSGYGRDDLCEAAEDAARHTLVVAMIEDAAGLRNADDIA
AQRGVDVLLEGAADLSQSLGVPWQTRHPHVRDAVDTIAAAALRHDKHFCALPRTPADAAAWRARGTRMMVLGDDRGIARR
AMAAHRQACIA
>Mature_251_residues
MKQRLLTPGSEPIVGLFCSTPAPLTVELIAAAGYDFAVIDLEHTLIDGALLGAMLLAARASGIAPLVRVAALHQVAPALD
AGAQGIVFPRITGAARASEAVAYCHHTCGIPAGKRGLNATWHSGYGRDDLCEAAEDAARHTLVVAMIEDAAGLRNADDIA
AQRGVDVLLEGAADLSQSLGVPWQTRHPHVRDAVDTIAAAALRHDKHFCALPRTPADAAAWRARGTRMMVLGDDRGIARR
AMAAHRQACIA

Specific function: Catalyzes the reversible retro-aldol cleavage of both 5- keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. KDGluc aldolase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=230, Percent_Identity=29.1304347826087, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788578, Length=200, Percent_Identity=30, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR017648
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.2.20 [H]

Molecular weight: Translated: 26303; Mature: 26303

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQRLLTPGSEPIVGLFCSTPAPLTVELIAAAGYDFAVIDLEHTLIDGALLGAMLLAARA
CCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHH
SGIAPLVRVAALHQVAPALDAGAQGIVFPRITGAARASEAVAYCHHTCGIPAGKRGLNAT
CCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
WHSGYGRDDLCEAAEDAARHTLVVAMIEDAAGLRNADDIAAQRGVDVLLEGAADLSQSLG
CCCCCCCHHHHHHHHHHHHHEEEEEEEHHHCCCCCHHHHHHHCCHHHHHHCHHHHHHHCC
VPWQTRHPHVRDAVDTIAAAALRHDKHFCALPRTPADAAAWRARGTRMMVLGDDRGIARR
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHCCCEEEEEECCCCHHHH
AMAAHRQACIA
HHHHHHHHHCC
>Mature Secondary Structure
MKQRLLTPGSEPIVGLFCSTPAPLTVELIAAAGYDFAVIDLEHTLIDGALLGAMLLAARA
CCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHH
SGIAPLVRVAALHQVAPALDAGAQGIVFPRITGAARASEAVAYCHHTCGIPAGKRGLNAT
CCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
WHSGYGRDDLCEAAEDAARHTLVVAMIEDAAGLRNADDIAAQRGVDVLLEGAADLSQSLG
CCCCCCCHHHHHHHHHHHHHEEEEEEEHHHCCCCCHHHHHHHCCHHHHHHCHHHHHHHCC
VPWQTRHPHVRDAVDTIAAAALRHDKHFCALPRTPADAAAWRARGTRMMVLGDDRGIARR
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHCCCEEEEEECCCCHHHH
AMAAHRQACIA
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA