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The map label for this gene is tilS [H]

Identifier: 94310033

GI number: 94310033

Start: 1179979

End: 1181442

Strand: Direct

Name: tilS [H]

Synonym: Rmet_1088

Alternate gene names: 94310033

Gene position: 1179979-1181442 (Clockwise)

Preceding gene: 94310032

Following gene: 335055515

Centisome position: 30.04

GC content: 68.31

Gene sequence:

>1464_bases
GTGAGCGCAGGGAATTCGACGCAACTGATCGCGCGTCTGACCGACAAGGTCGCACAGGCTCTACAGGCCAGTGCGACCTT
TGTTGTTTCTGGCGATAGGCGGTTGGAGACTGGTAGCCAACGGGCTGCCGACGGGCAGCCCGTGACCGTCGCGGTGGCGC
TGTCAGGTGGCCGTGACTCCGTGGCGCTGCTCCATGCCACGCGGGAAGCCCTGGCCGCCACGTGGCCGCTTGCGCGTCTG
GTGGCGCTGCACGTGCACCATGGCCTGCAGGCGCAGGCTGATGACTGGGACCGATTCTGCACCGCGCGGTGCGGCGATCT
GCAGGTGCCCTTGAGCAGCGCGCGCGTGACGGTGCAGGCGGCGACGGGCGAGGGCATAGAGGCCGCGGCGCGGCGAGCTC
GCCATGAGGCGCTGGGCAGGATGTGTCGCGAGCAGGGCGCACGGCTGCTGCTGTTCGCGCATCATCGTGACGATCAGGTG
GAAACCGTGCTGCTGCGGCTGTTCCGTGGAACGGGTGTGGCCGGCATGGCTGGAATGCCCGCGCGACGCCCGCTCGATGA
ACGGGGCGACATTCAACTGGTGCGGCCATGGCTCGGTGTGACACGCGCCGAGATCGACGATTACTGCGCGTGGCAGGGGT
TGCGCTGGATCGACGATCCCTCGAACGCGGACACGCGTTTCGCGCGCAATGCGCTGCGCGCGCAATTGCCTGCACTATTA
CAGGCGTTTCCGGCCCTCTATGAGAACGTGGCCCAGGCCGCCACGCATTTCGCACAGGCGGCCGGCATGCTCGATGCGCT
GGCCGCGCAATCGCTGGCTTCGCTGGTCTGCGCCGGGCGCGATGCCGACACCATTTCAGAACTTGATCTCGCGGGCCTGC
GCGCCTTGCCATCCGAGCACGCGGATGCCGTGCTGCGCCACTGGCTGCGCGATCTCGGCACCCAGTCGCCGTCGACGGCG
CGCCTGGCCGCGATGCGGGCCCAACTCATCCAGCATCAAGGTGGCGAGCCGGCCATCGCGCACGATGGCCTGGTGTTGCG
TCGTTTCCGTGACCGGCTGCTGGCCTGCCCAGCCGTGTCCGCTGGAATGCCGGCGCCGTTTGCATTCGAGTGGCGCGGCG
AATCGCGCATCGTAGTGCCGGCATGGCGTGGCGAACTGCACTTCGAGCGTGATGACACCTTTGGTGTGCCCGAGTCTGTG
CTGCGCGTGCCGTTGAGGCTGGCTGGCAGGGTAGGGGGCGAGCGCATCGTCCTGCGTCCGGGTGGCCCGGCGCGAGCGCT
GAAACAGGCCTATCAGGAGGCGGATATTCCCGCATGGCGGCGAGCGCATCTGCCGCTGCTATGGAGTGCCGATGTGCTGG
TCCTGGCGGCCGGTTTGGGCATGCATCGTCGCTGGCCCAACGATGCGCCACATGCCCCGCGGTGGCGCGTGGAGTGGCTC
GCACGGCCCGATCTGCGCGGCTAA

Upstream 100 bases:

>100_bases
CGCCAGTTCCAGGGCATGAGCGTCAAGGAACTGCAGGCGCGTCGTCACGAGCGACTGATGGCCTATGGCAAGTTCAAGGA
AACCGGCGCCCAGGAATGAT

Downstream 100 bases:

>100_bases
TCTCACGGTTTTCGGCGAAAAATGGGGACAGTATCATTTCGGCTTGTGAATTATTTTCACAAGAAAATGATGCAAAATCT
GCGGTGTTTTTGCCGGATTT

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MSAGNSTQLIARLTDKVAQALQASATFVVSGDRRLETGSQRAADGQPVTVAVALSGGRDSVALLHATREALAATWPLARL
VALHVHHGLQAQADDWDRFCTARCGDLQVPLSSARVTVQAATGEGIEAAARRARHEALGRMCREQGARLLLFAHHRDDQV
ETVLLRLFRGTGVAGMAGMPARRPLDERGDIQLVRPWLGVTRAEIDDYCAWQGLRWIDDPSNADTRFARNALRAQLPALL
QAFPALYENVAQAATHFAQAAGMLDALAAQSLASLVCAGRDADTISELDLAGLRALPSEHADAVLRHWLRDLGTQSPSTA
RLAAMRAQLIQHQGGEPAIAHDGLVLRRFRDRLLACPAVSAGMPAPFAFEWRGESRIVVPAWRGELHFERDDTFGVPESV
LRVPLRLAGRVGGERIVLRPGGPARALKQAYQEADIPAWRRAHLPLLWSADVLVLAAGLGMHRRWPNDAPHAPRWRVEWL
ARPDLRG

Sequences:

>Translated_487_residues
MSAGNSTQLIARLTDKVAQALQASATFVVSGDRRLETGSQRAADGQPVTVAVALSGGRDSVALLHATREALAATWPLARL
VALHVHHGLQAQADDWDRFCTARCGDLQVPLSSARVTVQAATGEGIEAAARRARHEALGRMCREQGARLLLFAHHRDDQV
ETVLLRLFRGTGVAGMAGMPARRPLDERGDIQLVRPWLGVTRAEIDDYCAWQGLRWIDDPSNADTRFARNALRAQLPALL
QAFPALYENVAQAATHFAQAAGMLDALAAQSLASLVCAGRDADTISELDLAGLRALPSEHADAVLRHWLRDLGTQSPSTA
RLAAMRAQLIQHQGGEPAIAHDGLVLRRFRDRLLACPAVSAGMPAPFAFEWRGESRIVVPAWRGELHFERDDTFGVPESV
LRVPLRLAGRVGGERIVLRPGGPARALKQAYQEADIPAWRRAHLPLLWSADVLVLAAGLGMHRRWPNDAPHAPRWRVEWL
ARPDLRG
>Mature_486_residues
SAGNSTQLIARLTDKVAQALQASATFVVSGDRRLETGSQRAADGQPVTVAVALSGGRDSVALLHATREALAATWPLARLV
ALHVHHGLQAQADDWDRFCTARCGDLQVPLSSARVTVQAATGEGIEAAARRARHEALGRMCREQGARLLLFAHHRDDQVE
TVLLRLFRGTGVAGMAGMPARRPLDERGDIQLVRPWLGVTRAEIDDYCAWQGLRWIDDPSNADTRFARNALRAQLPALLQ
AFPALYENVAQAATHFAQAAGMLDALAAQSLASLVCAGRDADTISELDLAGLRALPSEHADAVLRHWLRDLGTQSPSTAR
LAAMRAQLIQHQGGEPAIAHDGLVLRRFRDRLLACPAVSAGMPAPFAFEWRGESRIVVPAWRGELHFERDDTFGVPESVL
RVPLRLAGRVGGERIVLRPGGPARALKQAYQEADIPAWRRAHLPLLWSADVLVLAAGLGMHRRWPNDAPHAPRWRVEWLA
RPDLRG

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=417, Percent_Identity=32.3741007194245, Blast_Score=153, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 53060; Mature: 52929

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAGNSTQLIARLTDKVAQALQASATFVVSGDRRLETGSQRAADGQPVTVAVALSGGRDS
CCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCC
VALLHATREALAATWPLARLVALHVHHGLQAQADDWDRFCTARCGDLQVPLSSARVTVQA
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCEECCCCCCEEEEEE
ATGEGIEAAARRARHEALGRMCREQGARLLLFAHHRDDQVETVLLRLFRGTGVAGMAGMP
CCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCC
ARRPLDERGDIQLVRPWLGVTRAEIDDYCAWQGLRWIDDPSNADTRFARNALRAQLPALL
CCCCCCCCCCEEEECHHHCCHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHH
QAFPALYENVAQAATHFAQAAGMLDALAAQSLASLVCAGRDADTISELDLAGLRALPSEH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHH
ADAVLRHWLRDLGTQSPSTARLAAMRAQLIQHQGGEPAIAHDGLVLRRFRDRLLACPAVS
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCC
AGMPAPFAFEWRGESRIVVPAWRGELHFERDDTFGVPESVLRVPLRLAGRVGGERIVLRP
CCCCCCEEEEECCCCEEEEEECCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEECC
GGPARALKQAYQEADIPAWRRAHLPLLWSADVLVLAAGLGMHRRWPNDAPHAPRWRVEWL
CCHHHHHHHHHHHCCCCCHHHCCCCEEECCCHHHHHHCCCCCCCCCCCCCCCCCEEEEEE
ARPDLRG
CCCCCCC
>Mature Secondary Structure 
SAGNSTQLIARLTDKVAQALQASATFVVSGDRRLETGSQRAADGQPVTVAVALSGGRDS
CCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCC
VALLHATREALAATWPLARLVALHVHHGLQAQADDWDRFCTARCGDLQVPLSSARVTVQA
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCEECCCCCCEEEEEE
ATGEGIEAAARRARHEALGRMCREQGARLLLFAHHRDDQVETVLLRLFRGTGVAGMAGMP
CCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCC
ARRPLDERGDIQLVRPWLGVTRAEIDDYCAWQGLRWIDDPSNADTRFARNALRAQLPALL
CCCCCCCCCCEEEECHHHCCHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHH
QAFPALYENVAQAATHFAQAAGMLDALAAQSLASLVCAGRDADTISELDLAGLRALPSEH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHH
ADAVLRHWLRDLGTQSPSTARLAAMRAQLIQHQGGEPAIAHDGLVLRRFRDRLLACPAVS
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCC
AGMPAPFAFEWRGESRIVVPAWRGELHFERDDTFGVPESVLRVPLRLAGRVGGERIVLRP
CCCCCCEEEEECCCCEEEEEECCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEECC
GGPARALKQAYQEADIPAWRRAHLPLLWSADVLVLAAGLGMHRRWPNDAPHAPRWRVEWL
CCHHHHHHHHHHHCCCCCHHHCCCCEEECCCHHHHHHCCCCCCCCCCCCCCCCCEEEEEE
ARPDLRG
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]