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The map label for this gene is eno

Identifier: 94310000

GI number: 94310000

Start: 1148371

End: 1149660

Strand: Direct

Name: eno

Synonym: Rmet_1055

Alternate gene names: 94310000

Gene position: 1148371-1149660 (Clockwise)

Preceding gene: 94309999

Following gene: 94310001

Centisome position: 29.23

GC content: 62.79

Gene sequence:

>1290_bases
ATGAGTGCAATCGTAGATATCATCGGTCGCGAGGTTCTCGACTCGCGCGGCAATCCCACCGTCGAGTGCGACGTGCTGCT
GGAGTCGGGCGTGATGGGCCGTGCTGCCGTACCGTCGGGTGCATCGACCGGTTCGCGCGAAGCCATCGAACTGCGTGATG
GCGACAAGTCGCGCTACCTGGGCAAGGGCGTGCTGAAGGCTGTCGAGCACATCAACACCGAGATCTCCGAAGCGATCATG
GGCCTGGATGCCTCCGAGCAGGCTTTCCTGGACCGCACGCTGATCGAACTGGACGGCACCGAGAACAAGGGCCGCCTGGG
CGCCAACGCCATGCTGGCTGTGTCGATGGCCGTGGCCAAGGCAGCCGCCGAGGAAGCCGGCCTGCCGCTGTACCGCTACT
TCGGCGGTTCGGGCGCGATGCAGATGCCGGTGCCGATGATGAACATCGTCAACGGTGGCGCGCACGCCAACAACAGCCTG
GACATCCAGGAATTCATGATCATGCCCGTGTCGCAGACCAGCTTCCGTGAAGCTCTGCGTTGCGGCGCCGAAGTGTTCCA
CGCGCTGAAGAAGATCCTGTCCGACAAGGGCATGTCCACCGCCGTGGGCGACGAGGGCGGCTTCGCCCCGAACTTCTCGT
CGAACGAGGAGTGCCTGAACACGGTTGTGCAGGCCATCGAAAAGGCTGGCTACAAGGCTGGCGAGGACGTGCTGCTGGCG
CTGGACTGCGCCGCCAGCGAGTTCTACCACGAAGCCGAAGGCGTGTACTCGCTGGAAGGCGAAGGCCTGAAGCTGACGTC
GACCCAGTTCGCCGACTACCTGGCCAACCTGTGCGACAAGTTCCCGATCGTGTCGATCGAGGACGGCATGGCCGAAGGCG
ACTGGGAAGGCTGGAAGACGCTGACCGACAAGCTGGGCAAGCGCGTCCAACTGGTGGGTGACGACCTGTTCGTGACCAAC
ACGAAGATCCTGAAGGAAGGCATCGAGAAGGGCATCGGCAACTCGATTCTCATCAAGATCAACCAGATCGGCACGCTGAC
CGAGACGTTCGCTGCTATCGAGATGGCCAAGCGCGCCGGTTACACCGCCGTGATCTCGCACCGTTCGGGTGAGACCGAAG
ACAGCACGATCGCCGATATCGCCGTCGGCACGAACGCCGGCCAGATCAAGACCGGTTCGCTGTCGCGCTCGGACCGCATC
TCGAAGTACAACCAGCTGCTGCGTATCGAGGAAGACCTGGGCGACATCGCCACGTACCCGGGCAAGAGCACGTTCTACAA
CCTGCGCTGA

Upstream 100 bases:

>100_bases
GCGAAGATGAACATGATCGTCGTGGAAGGCATCTGACATATTCGTCGGGCATCCTGCGCGGTAAGAAGATTCACCAGATT
CAACAGTCCAGAGAGGAAAT

Downstream 100 bases:

>100_bases
TATCGCCGGTTAGTGTTCTGAGTTCCAGAAAAAGCGCCGCCCCGGAGCACCGGGGCGGCGCTTTTTTTGTTGTCAACTGG
ATGCCTGTTGCGCCGTTGTT

Product: enolase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase 1; 2-phosphoglycerate dehydratase 1

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSAIVDIIGREVLDSRGNPTVECDVLLESGVMGRAAVPSGASTGSREAIELRDGDKSRYLGKGVLKAVEHINTEISEAIM
GLDASEQAFLDRTLIELDGTENKGRLGANAMLAVSMAVAKAAAEEAGLPLYRYFGGSGAMQMPVPMMNIVNGGAHANNSL
DIQEFMIMPVSQTSFREALRCGAEVFHALKKILSDKGMSTAVGDEGGFAPNFSSNEECLNTVVQAIEKAGYKAGEDVLLA
LDCAASEFYHEAEGVYSLEGEGLKLTSTQFADYLANLCDKFPIVSIEDGMAEGDWEGWKTLTDKLGKRVQLVGDDLFVTN
TKILKEGIEKGIGNSILIKINQIGTLTETFAAIEMAKRAGYTAVISHRSGETEDSTIADIAVGTNAGQIKTGSLSRSDRI
SKYNQLLRIEEDLGDIATYPGKSTFYNLR

Sequences:

>Translated_429_residues
MSAIVDIIGREVLDSRGNPTVECDVLLESGVMGRAAVPSGASTGSREAIELRDGDKSRYLGKGVLKAVEHINTEISEAIM
GLDASEQAFLDRTLIELDGTENKGRLGANAMLAVSMAVAKAAAEEAGLPLYRYFGGSGAMQMPVPMMNIVNGGAHANNSL
DIQEFMIMPVSQTSFREALRCGAEVFHALKKILSDKGMSTAVGDEGGFAPNFSSNEECLNTVVQAIEKAGYKAGEDVLLA
LDCAASEFYHEAEGVYSLEGEGLKLTSTQFADYLANLCDKFPIVSIEDGMAEGDWEGWKTLTDKLGKRVQLVGDDLFVTN
TKILKEGIEKGIGNSILIKINQIGTLTETFAAIEMAKRAGYTAVISHRSGETEDSTIADIAVGTNAGQIKTGSLSRSDRI
SKYNQLLRIEEDLGDIATYPGKSTFYNLR
>Mature_428_residues
SAIVDIIGREVLDSRGNPTVECDVLLESGVMGRAAVPSGASTGSREAIELRDGDKSRYLGKGVLKAVEHINTEISEAIMG
LDASEQAFLDRTLIELDGTENKGRLGANAMLAVSMAVAKAAAEEAGLPLYRYFGGSGAMQMPVPMMNIVNGGAHANNSLD
IQEFMIMPVSQTSFREALRCGAEVFHALKKILSDKGMSTAVGDEGGFAPNFSSNEECLNTVVQAIEKAGYKAGEDVLLAL
DCAASEFYHEAEGVYSLEGEGLKLTSTQFADYLANLCDKFPIVSIEDGMAEGDWEGWKTLTDKLGKRVQLVGDDLFVTNT
KILKEGIEKGIGNSILIKINQIGTLTETFAAIEMAKRAGYTAVISHRSGETEDSTIADIAVGTNAGQIKTGSLSRSDRIS
KYNQLLRIEEDLGDIATYPGKSTFYNLR

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=439, Percent_Identity=51.0250569476082, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI4503571, Length=433, Percent_Identity=49.4226327944573, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI301897477, Length=432, Percent_Identity=49.7685185185185, Blast_Score=407, Evalue=1e-113,
Organism=Homo sapiens, GI301897469, Length=432, Percent_Identity=49.7685185185185, Blast_Score=407, Evalue=1e-113,
Organism=Homo sapiens, GI301897479, Length=430, Percent_Identity=44.8837209302326, Blast_Score=350, Evalue=2e-96,
Organism=Homo sapiens, GI169201331, Length=351, Percent_Identity=25.6410256410256, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI169201757, Length=351, Percent_Identity=25.6410256410256, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI239744207, Length=351, Percent_Identity=25.6410256410256, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI310129182, Length=136, Percent_Identity=30.1470588235294, Blast_Score=65, Evalue=9e-11,
Organism=Homo sapiens, GI310110045, Length=136, Percent_Identity=30.1470588235294, Blast_Score=65, Evalue=9e-11,
Organism=Homo sapiens, GI310120572, Length=136, Percent_Identity=30.1470588235294, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1789141, Length=427, Percent_Identity=65.3395784543326, Blast_Score=554, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI17536383, Length=439, Percent_Identity=49.6583143507973, Blast_Score=405, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI71995829, Length=439, Percent_Identity=49.6583143507973, Blast_Score=405, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI32563855, Length=199, Percent_Identity=42.2110552763819, Blast_Score=159, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6321693, Length=439, Percent_Identity=46.9248291571754, Blast_Score=381, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6323985, Length=434, Percent_Identity=47.4654377880184, Blast_Score=372, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6324974, Length=434, Percent_Identity=47.4654377880184, Blast_Score=371, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6324969, Length=434, Percent_Identity=47.4654377880184, Blast_Score=371, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6321968, Length=439, Percent_Identity=46.4692482915718, Blast_Score=357, Evalue=2e-99,
Organism=Drosophila melanogaster, GI24580918, Length=430, Percent_Identity=51.3953488372093, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580916, Length=430, Percent_Identity=51.3953488372093, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580920, Length=430, Percent_Identity=51.3953488372093, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580914, Length=430, Percent_Identity=51.3953488372093, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI281360527, Length=430, Percent_Identity=51.3953488372093, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI17137654, Length=430, Percent_Identity=51.3953488372093, Blast_Score=395, Evalue=1e-110,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO1_RALME (Q1LPI5)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_583210.1
- ProteinModelPortal:   Q1LPI5
- SMR:   Q1LPI5
- STRING:   Q1LPI5
- GeneID:   4037852
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_1055
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- PhylomeDB:   Q1LPI5
- ProtClustDB:   PRK00077
- BioCyc:   RMET266264:RMET_1055-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45939; Mature: 45807

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 339-339 BINDING 155-155 BINDING 164-164 BINDING 287-287 BINDING 314-314 BINDING 339-339 BINDING 390-390

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIVDIIGREVLDSRGNPTVECDVLLESGVMGRAAVPSGASTGSREAIELRDGDKSRYL
CCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHH
GKGVLKAVEHINTEISEAIMGLDASEQAFLDRTLIELDGTENKGRLGANAMLAVSMAVAK
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHH
AAAEEAGLPLYRYFGGSGAMQMPVPMMNIVNGGAHANNSLDIQEFMIMPVSQTSFREALR
HHHHHCCCCEEEEECCCCCEECCCHHHHHHCCCCCCCCCCCHHHHEEECCCHHHHHHHHH
CGAEVFHALKKILSDKGMSTAVGDEGGFAPNFSSNEECLNTVVQAIEKAGYKAGEDVLLA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDCAASEFYHEAEGVYSLEGEGLKLTSTQFADYLANLCDKFPIVSIEDGMAEGDWEGWKT
EEHHHHHHHHHHCCEEEECCCCEEEEHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHH
LTDKLGKRVQLVGDDLFVTNTKILKEGIEKGIGNSILIKINQIGTLTETFAAIEMAKRAG
HHHHHCCEEEEECCCEEECHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCC
YTAVISHRSGETEDSTIADIAVGTNAGQIKTGSLSRSDRISKYNQLLRIEEDLGDIATYP
CEEEEECCCCCCCCCEEEEEEECCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
GKSTFYNLR
CCCCEECCC
>Mature Secondary Structure 
SAIVDIIGREVLDSRGNPTVECDVLLESGVMGRAAVPSGASTGSREAIELRDGDKSRYL
CHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHH
GKGVLKAVEHINTEISEAIMGLDASEQAFLDRTLIELDGTENKGRLGANAMLAVSMAVAK
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHH
AAAEEAGLPLYRYFGGSGAMQMPVPMMNIVNGGAHANNSLDIQEFMIMPVSQTSFREALR
HHHHHCCCCEEEEECCCCCEECCCHHHHHHCCCCCCCCCCCHHHHEEECCCHHHHHHHHH
CGAEVFHALKKILSDKGMSTAVGDEGGFAPNFSSNEECLNTVVQAIEKAGYKAGEDVLLA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDCAASEFYHEAEGVYSLEGEGLKLTSTQFADYLANLCDKFPIVSIEDGMAEGDWEGWKT
EEHHHHHHHHHHCCEEEECCCCEEEEHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHH
LTDKLGKRVQLVGDDLFVTNTKILKEGIEKGIGNSILIKINQIGTLTETFAAIEMAKRAG
HHHHHCCEEEEECCCEEECHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCC
YTAVISHRSGETEDSTIADIAVGTNAGQIKTGSLSRSDRISKYNQLLRIEEDLGDIATYP
CEEEEECCCCCCCCCEEEEEEECCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
GKSTFYNLR
CCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA