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The map label for this gene is lysS [H]

Identifier: 94309979

GI number: 94309979

Start: 1122634

End: 1124181

Strand: Reverse

Name: lysS [H]

Synonym: Rmet_1034

Alternate gene names: 94309979

Gene position: 1124181-1122634 (Counterclockwise)

Preceding gene: 94309980

Following gene: 94309978

Centisome position: 28.62

GC content: 63.76

Gene sequence:

>1548_bases
ATGACCGAACCGACCCGCGCCCAAGCCGCCACGCCCACCACCGAAGGGCTTGCCGTAGATGAGAACAAGATCATCGCCGA
GCGGCGCGAGAAACTGCAGGCCCTGCGCCAGCAAGGCGTGGCCTTCCCCAACGATTTCCAGCCGACGCACCAGGCCGGCG
CGCTGCACGCGCAGTATGGCGAAACGGACCAGGCCACGCTGGAAGCCAGCCCCGTGGAAGTGTCCATCGCCGGACGCATG
ATGCTCAAGCGCGTGATGGGCAAGGCCAGCTTCGCCACGGTCCAGGACGGCAGCGGCCAGATCCAGTTCTACATCACGCG
CGACAAGGTCGGTGAAGACGTCTATGCCGCGTTCAAGCACTGGGACCTCGGCGACATCATCAGCGCGCGCGGAGAACTGT
TCCGCACGAACAAGGGCGAGTTGTCGGTGCAGGTGCGCGAACTGCGCCTGCTGTCGAAGGCACTGCGTCCCCTGCCGGAC
AAGTTCCATGGCCTGGCCGACCAGGAAACCAAATACCGCCAGCGCTATGTGGACCTGATCGTCTCGCCGGAAACGCGCAG
CACGTTCCGCGCCCGCACCCAGGCCATCTCGTCGATCCGCCGCCATATGGCCGATGCCGGCTTTATGGAAGTGGAAACGC
CGATGCTGCACCCGATTCCGGGTGGCGCGGCGGCCAAGCCGTTCATCACGCACCACAACGCACTGGACATGCAGATGTTC
ATGCGCATCGCGCCCGAGCTCTATCTGAAGCGCCTGATCGTCGGCGGCTTCGAGCGCGTGTTCGAAATCAACCGCAACTT
CCGTAACGAAGGGGTGAGCCCGCGCCACAACCCCGAGTTCACGATGATGGAGTTCTACGCGGCCTACACGGATTACCGTT
GGCTGATGGACTTCACCGAGAACCTGATCCGTCAGGCCGCAATTGACGCGCGTGGCAGCGCCACGGTGACGTACCAGGGC
CGCGAACTTGATCTGTCCAAGCCGTTCCATCGCCTGACGATCTGCGGGGCGATCCAGAAGTACGCGCCGGAATACACCGA
CGCGCAGCTTGCCGATGCCGACTTCCTGCGCGCCGAGCTCAAGAACAAGTTCAAGATCAATACCAGCGCGCCGCAGTTCC
TGAACGCCGGCCTGGGCACGCTGCAACTGGTGCTCTTCGAGGAAACCGCCGAAGCGCAACTCTGGGAACCGACCTACATC
GTCGACTACCCGGTCGAGGTATCGCCGCTGGCGCGCGGCTCGGACACGCAGCCGGGCATTACCGAACGCTTCGAACTGTT
CATCACGGGCCGCGAGATCGCCAACGGCTTCTCCGAGCTTAACGACCCGGAAGACCAGGCCGAACGTTTCCGCAAGCAGG
TCGAGCAGAAGGATGCGGGCGACGAGGAAGCGATGTACTTCGACGCCGATTACATCCGCGCGCTCGAGTACGGCATGCCT
CCGACAGGCGGCTGCGGTATCGGCATCGACCGCCTGGTGATGCTGCTGACCGACAGCCCGAACATCCGCGACGTAATCCT
GTTCCCGCACCTGCGCCGCGAGGACTGA

Upstream 100 bases:

>100_bases
ATTTCGGCACCACCGAACTCGACGACATCCGCAAATACTGAACCGATACATTGAACCGAACCCGCGCGACGCTCCGCGCC
GCGCGATCGAACCTCACATC

Downstream 100 bases:

>100_bases
TTCTCAAGGCCGGTCCCACCGGCCGGTGAGAATATGACCTTGAACGAGAGCCCCGCCCACGCGGGGCTTTTGCTTTGTTG
GGGCGCCCGGCCACTGCACA

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MTEPTRAQAATPTTEGLAVDENKIIAERREKLQALRQQGVAFPNDFQPTHQAGALHAQYGETDQATLEASPVEVSIAGRM
MLKRVMGKASFATVQDGSGQIQFYITRDKVGEDVYAAFKHWDLGDIISARGELFRTNKGELSVQVRELRLLSKALRPLPD
KFHGLADQETKYRQRYVDLIVSPETRSTFRARTQAISSIRRHMADAGFMEVETPMLHPIPGGAAAKPFITHHNALDMQMF
MRIAPELYLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTENLIRQAAIDARGSATVTYQG
RELDLSKPFHRLTICGAIQKYAPEYTDAQLADADFLRAELKNKFKINTSAPQFLNAGLGTLQLVLFEETAEAQLWEPTYI
VDYPVEVSPLARGSDTQPGITERFELFITGREIANGFSELNDPEDQAERFRKQVEQKDAGDEEAMYFDADYIRALEYGMP
PTGGCGIGIDRLVMLLTDSPNIRDVILFPHLRRED

Sequences:

>Translated_515_residues
MTEPTRAQAATPTTEGLAVDENKIIAERREKLQALRQQGVAFPNDFQPTHQAGALHAQYGETDQATLEASPVEVSIAGRM
MLKRVMGKASFATVQDGSGQIQFYITRDKVGEDVYAAFKHWDLGDIISARGELFRTNKGELSVQVRELRLLSKALRPLPD
KFHGLADQETKYRQRYVDLIVSPETRSTFRARTQAISSIRRHMADAGFMEVETPMLHPIPGGAAAKPFITHHNALDMQMF
MRIAPELYLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTENLIRQAAIDARGSATVTYQG
RELDLSKPFHRLTICGAIQKYAPEYTDAQLADADFLRAELKNKFKINTSAPQFLNAGLGTLQLVLFEETAEAQLWEPTYI
VDYPVEVSPLARGSDTQPGITERFELFITGREIANGFSELNDPEDQAERFRKQVEQKDAGDEEAMYFDADYIRALEYGMP
PTGGCGIGIDRLVMLLTDSPNIRDVILFPHLRRED
>Mature_514_residues
TEPTRAQAATPTTEGLAVDENKIIAERREKLQALRQQGVAFPNDFQPTHQAGALHAQYGETDQATLEASPVEVSIAGRMM
LKRVMGKASFATVQDGSGQIQFYITRDKVGEDVYAAFKHWDLGDIISARGELFRTNKGELSVQVRELRLLSKALRPLPDK
FHGLADQETKYRQRYVDLIVSPETRSTFRARTQAISSIRRHMADAGFMEVETPMLHPIPGGAAAKPFITHHNALDMQMFM
RIAPELYLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTENLIRQAAIDARGSATVTYQGR
ELDLSKPFHRLTICGAIQKYAPEYTDAQLADADFLRAELKNKFKINTSAPQFLNAGLGTLQLVLFEETAEAQLWEPTYIV
DYPVEVSPLARGSDTQPGITERFELFITGREIANGFSELNDPEDQAERFRKQVEQKDAGDEEAMYFDADYIRALEYGMPP
TGGCGIGIDRLVMLLTDSPNIRDVILFPHLRRED

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=513, Percent_Identity=39.3762183235867, Blast_Score=357, Evalue=2e-98,
Organism=Homo sapiens, GI194272210, Length=513, Percent_Identity=39.3762183235867, Blast_Score=356, Evalue=3e-98,
Organism=Homo sapiens, GI40789249, Length=292, Percent_Identity=22.9452054794521, Blast_Score=83, Evalue=7e-16,
Organism=Escherichia coli, GI1789256, Length=496, Percent_Identity=60.0806451612903, Blast_Score=610, Evalue=1e-176,
Organism=Escherichia coli, GI1790571, Length=496, Percent_Identity=59.8790322580645, Blast_Score=607, Evalue=1e-175,
Organism=Escherichia coli, GI87082379, Length=327, Percent_Identity=31.4984709480122, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1788173, Length=251, Percent_Identity=25.4980079681275, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17535925, Length=497, Percent_Identity=40.8450704225352, Blast_Score=353, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI17535927, Length=497, Percent_Identity=40.8450704225352, Blast_Score=353, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI71994340, Length=512, Percent_Identity=40.4296875, Blast_Score=353, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI32566633, Length=299, Percent_Identity=24.7491638795987, Blast_Score=85, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6320242, Length=521, Percent_Identity=41.4587332053743, Blast_Score=355, Evalue=1e-98,
Organism=Saccharomyces cerevisiae, GI6324256, Length=563, Percent_Identity=31.0834813499112, Blast_Score=187, Evalue=4e-48,
Organism=Drosophila melanogaster, GI24640849, Length=527, Percent_Identity=40.0379506641366, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24640851, Length=527, Percent_Identity=40.0379506641366, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24584738, Length=342, Percent_Identity=24.8538011695906, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 58291; Mature: 58160

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPTRAQAATPTTEGLAVDENKIIAERREKLQALRQQGVAFPNDFQPTHQAGALHAQYG
CCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCEECCCC
ETDQATLEASPVEVSIAGRMMLKRVMGKASFATVQDGSGQIQFYITRDKVGEDVYAAFKH
CCCCCEEECCCEEEEHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHC
WDLGDIISARGELFRTNKGELSVQVRELRLLSKALRPLPDKFHGLADQETKYRQRYVDLI
CCHHHHHHHCCCEEECCCCCEEEHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHE
VSPETRSTFRARTQAISSIRRHMADAGFMEVETPMLHPIPGGAAAKPFITHHNALDMQMF
ECCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCEEECCCHHHHHHH
MRIAPELYLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
NLIRQAAIDARGSATVTYQGRELDLSKPFHRLTICGAIQKYAPEYTDAQLADADFLRAEL
HHHHHHHCCCCCCEEEEECCCEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
KNKFKINTSAPQFLNAGLGTLQLVLFEETAEAQLWEPTYIVDYPVEVSPLARGSDTQPGI
CCCEEECCCCHHHHHCCCCCEEEEEEECCCCCEECCCEEEEECCCEECCCCCCCCCCCCC
TERFELFITGREIANGFSELNDPEDQAERFRKQVEQKDAGDEEAMYFDADYIRALEYGMP
CCEEEEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHCCCC
PTGGCGIGIDRLVMLLTDSPNIRDVILFPHLRRED
CCCCCCCCHHHEEEEEECCCCCCEEEEECCCCCCC
>Mature Secondary Structure 
TEPTRAQAATPTTEGLAVDENKIIAERREKLQALRQQGVAFPNDFQPTHQAGALHAQYG
CCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCEECCCC
ETDQATLEASPVEVSIAGRMMLKRVMGKASFATVQDGSGQIQFYITRDKVGEDVYAAFKH
CCCCCEEECCCEEEEHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHC
WDLGDIISARGELFRTNKGELSVQVRELRLLSKALRPLPDKFHGLADQETKYRQRYVDLI
CCHHHHHHHCCCEEECCCCCEEEHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHE
VSPETRSTFRARTQAISSIRRHMADAGFMEVETPMLHPIPGGAAAKPFITHHNALDMQMF
ECCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCEEECCCHHHHHHH
MRIAPELYLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
NLIRQAAIDARGSATVTYQGRELDLSKPFHRLTICGAIQKYAPEYTDAQLADADFLRAEL
HHHHHHHCCCCCCEEEEECCCEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
KNKFKINTSAPQFLNAGLGTLQLVLFEETAEAQLWEPTYIVDYPVEVSPLARGSDTQPGI
CCCEEECCCCHHHHHCCCCCEEEEEEECCCCCEECCCEEEEECCCEECCCCCCCCCCCCC
TERFELFITGREIANGFSELNDPEDQAERFRKQVEQKDAGDEEAMYFDADYIRALEYGMP
CCEEEEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHCCCC
PTGGCGIGIDRLVMLLTDSPNIRDVILFPHLRRED
CCCCCCCCHHHEEEEEECCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA