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The map label for this gene is iscA [H]

Identifier: 94309972

GI number: 94309972

Start: 1117060

End: 1117401

Strand: Direct

Name: iscA [H]

Synonym: Rmet_1027

Alternate gene names: 94309972

Gene position: 1117060-1117401 (Clockwise)

Preceding gene: 94309971

Following gene: 94309973

Centisome position: 28.44

GC content: 60.23

Gene sequence:

>342_bases
GTGTTCGACAGCAAGGCAAAGATGATCTCAATGACCGAAAAGGCGGCCAAGCACGTCGCCCGATACCTGGAACGCCGCGG
CAAGGGCGTGGGCCTGCGCGTGGGCGTGAAGACGACTGGCTGTTCGGGTCTGGCGTACAAGCTTGAGTACGTCGATGAAG
TGCTGCCCGAAGACCAGGTGTTCGAAACGCACGGCATCAAGGTGATCGTGGACCCGAAGAGCCTGCCGTACATCGACGGC
ACCGAGCTGGATTTCGCACGCGAAGGGTTGAACGAAGGCTTCAAGTTCAACAACCCCAACGTCAAGGACGAGTGCGGCTG
CGGCGAGTCGTTCCGGGTCTGA

Upstream 100 bases:

>100_bases
TCGATGATTACAAGAAGAAGCACCCGGGCGCCGAGCAGAAGGCTGCCTGATCCGGCGTAGACGGTTTGCATGACCGCGCG
TGGCAAGTGGGTCGCGCGCG

Downstream 100 bases:

>100_bases
CGGCTTTTGCCGCGAGGCATGCAACAGGGGGCGGCATAGCCGCTCCTTTTTGTTGGGGAAGCTCTTGAAAGACGATTTTT
TTGCGCTGTTCGGGCTGCCT

Product: FeS cluster assembly protein

Products: NA

Alternate protein names: Iron-sulfur cluster assembly protein [H]

Number of amino acids: Translated: 113; Mature: 113

Protein sequence:

>113_residues
MFDSKAKMISMTEKAAKHVARYLERRGKGVGLRVGVKTTGCSGLAYKLEYVDEVLPEDQVFETHGIKVIVDPKSLPYIDG
TELDFAREGLNEGFKFNNPNVKDECGCGESFRV

Sequences:

>Translated_113_residues
MFDSKAKMISMTEKAAKHVARYLERRGKGVGLRVGVKTTGCSGLAYKLEYVDEVLPEDQVFETHGIKVIVDPKSLPYIDG
TELDFAREGLNEGFKFNNPNVKDECGCGESFRV
>Mature_113_residues
MFDSKAKMISMTEKAAKHVARYLERRGKGVGLRVGVKTTGCSGLAYKLEYVDEVLPEDQVFETHGIKVIVDPKSLPYIDG
TELDFAREGLNEGFKFNNPNVKDECGCGESFRV

Specific function: Is able to transfer iron-sulfur clusters to apo- ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that iscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transi

COG id: COG0316

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesB/iscA family [H]

Homologues:

Organism=Homo sapiens, GI52426768, Length=105, Percent_Identity=38.0952380952381, Blast_Score=89, Evalue=7e-19,
Organism=Homo sapiens, GI160420328, Length=96, Percent_Identity=38.5416666666667, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1788877, Length=105, Percent_Identity=66.6666666666667, Blast_Score=153, Evalue=2e-39,
Organism=Escherichia coli, GI1787974, Length=111, Percent_Identity=46.8468468468468, Blast_Score=112, Evalue=7e-27,
Organism=Escherichia coli, GI1786351, Length=116, Percent_Identity=37.9310344827586, Blast_Score=73, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI193209025, Length=105, Percent_Identity=37.1428571428571, Blast_Score=88, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI25146411, Length=105, Percent_Identity=37.1428571428571, Blast_Score=88, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6323001, Length=106, Percent_Identity=42.4528301886792, Blast_Score=89, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24642263, Length=103, Percent_Identity=40.7766990291262, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000361
- InterPro:   IPR016092
- InterPro:   IPR017870
- InterPro:   IPR011302 [H]

Pfam domain/function: PF01521 Fe-S_biosyn [H]

EC number: NA

Molecular weight: Translated: 12578; Mature: 12578

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01152 HESB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDSKAKMISMTEKAAKHVARYLERRGKGVGLRVGVKTTGCSGLAYKLEYVDEVLPEDQV
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECEECCCCCHHHHHHHHHHHCCCCCE
FETHGIKVIVDPKSLPYIDGTELDFAREGLNEGFKFNNPNVKDECGCGESFRV
EECCCCEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFDSKAKMISMTEKAAKHVARYLERRGKGVGLRVGVKTTGCSGLAYKLEYVDEVLPEDQV
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECEECCCCCHHHHHHHHHHHCCCCCE
FETHGIKVIVDPKSLPYIDGTELDFAREGLNEGFKFNNPNVKDECGCGESFRV
EECCCCEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12775707 [H]