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The map label for this gene is iscS [H]

Identifier: 94309970

GI number: 94309970

Start: 1115331

End: 1116551

Strand: Direct

Name: iscS [H]

Synonym: Rmet_1025

Alternate gene names: 94309970

Gene position: 1115331-1116551 (Clockwise)

Preceding gene: 94309969

Following gene: 94309971

Centisome position: 28.39

GC content: 62.57

Gene sequence:

>1221_bases
ATGAGCACCACCCCACATTTCCCGATCTACATGGATTACTCGGCAACGACGCCGGTGGACCCGCGCGTGGCCGACAAGAT
GATTCCGTACCTGCGCGAGCAGTTCGGTAATCCGGCGTCGCGTAGCCACGCGTATGGCTGGGACGCCGAGCGCGCCGTGG
AAGAGGCGCGCGAGCAGGTGGCCGCGCTGGTCGACGCCGATCCGCGCGAAATCGTCTGGACGTCGGGCGCGACCGAGTCG
GACAACCTCGCCATCAAGGGCGCGGCGAATTTCTACTCGGGCAAGGGCAAGCACATCATCACCGTGAAGACCGAGCACAA
GGCCGTGCTCGATACCACGCGCGAACTGGAGCGTCAGGGCTTCGAGGTGACGTACCTCGACGTCAAGGATGACGGCCTGA
TCGACATGGATGTGTTCAAGCAGGCGATTCGCCCGGACACGATCCTGGTGTCGGTGATGCTCGTGAACAATGAGATTGGT
GTGATCCAGGACGTCGAGCAGATCGGTGAGATCTGCCGCGAGAAGGGCATCATCTTCCATTGCGACGCCGCGCAGGCGAC
CGGCAAGGTGACCATCGACCTGAGCACGCTGAAGTGCGACCTGATGTCGTTCTCGGCCCACAAGACCTATGGTCCGAAGG
GCATCGGCGCGCTGTATGTTCGCCGCAAGCCGCGCGTGCGCATCGAAGCGCAAATGCACGGCGGCGGTCACGAGCGTGGC
ATGCGTTCGGGCACGCTGGCCCCGCACCAGATCGTCGGCATGGGCGAGGCATTCCGTCTGGCCCGCGAGGAAATGGCCAC
GGAAAACGAACGTATCCGGATGCTGCGCGACCGCCTGTGGAATGGTCTGTCGAGCATGGAAGAGGTGTATCTGAACGGTT
CGATGGAACACCGCGTGCCGCACAACCTCAACGTCAGCTTCAACTTCGTCGAGGGTGAGTCGCTGATCATGGCGATCAAG
GATGTGGCCGTGTCGTCGGGTTCGGCCTGCACGTCGGCTTCGCTGGAGCCGTCGTACGTGCTGCGCGCCCTTGGGCGCAA
TGACGAACTGGCGCACAGCTCGATCCGTTTCACCGTGGGCCGCTTCACGACCGAAGCCGAAATCGACTACACCGTCGAAC
TGCTCAAGAACAAGATCGGCAAGCTGCGCGACCTGTCGCCGCTGTGGGAAATGTTCAAGGACGGCGTCGACCTGAATTCG
ATCCAGTGGGCCGCGCACTGA

Upstream 100 bases:

>100_bases
GGTATTCAGCCTCGCGCAGTCCTGAGCAGGGATTTCGCGGCTGCAGTAAACTATCGGTAGACATTAAGCGATTAGCTGTA
CCTATAAGGCAGTCATAACG

Downstream 100 bases:

>100_bases
TTGCGGCACACGTAAAAAGAACAACCCCATTAAAAGATTGATTAGAGGTAGCCAAAATGTCTTACAGCAACAAGGTTCTC
GACCACTATGAAAATCCGCG

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MSTTPHFPIYMDYSATTPVDPRVADKMIPYLREQFGNPASRSHAYGWDAERAVEEAREQVAALVDADPREIVWTSGATES
DNLAIKGAANFYSGKGKHIITVKTEHKAVLDTTRELERQGFEVTYLDVKDDGLIDMDVFKQAIRPDTILVSVMLVNNEIG
VIQDVEQIGEICREKGIIFHCDAAQATGKVTIDLSTLKCDLMSFSAHKTYGPKGIGALYVRRKPRVRIEAQMHGGGHERG
MRSGTLAPHQIVGMGEAFRLAREEMATENERIRMLRDRLWNGLSSMEEVYLNGSMEHRVPHNLNVSFNFVEGESLIMAIK
DVAVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTVGRFTTEAEIDYTVELLKNKIGKLRDLSPLWEMFKDGVDLNS
IQWAAH

Sequences:

>Translated_406_residues
MSTTPHFPIYMDYSATTPVDPRVADKMIPYLREQFGNPASRSHAYGWDAERAVEEAREQVAALVDADPREIVWTSGATES
DNLAIKGAANFYSGKGKHIITVKTEHKAVLDTTRELERQGFEVTYLDVKDDGLIDMDVFKQAIRPDTILVSVMLVNNEIG
VIQDVEQIGEICREKGIIFHCDAAQATGKVTIDLSTLKCDLMSFSAHKTYGPKGIGALYVRRKPRVRIEAQMHGGGHERG
MRSGTLAPHQIVGMGEAFRLAREEMATENERIRMLRDRLWNGLSSMEEVYLNGSMEHRVPHNLNVSFNFVEGESLIMAIK
DVAVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTVGRFTTEAEIDYTVELLKNKIGKLRDLSPLWEMFKDGVDLNS
IQWAAH
>Mature_405_residues
STTPHFPIYMDYSATTPVDPRVADKMIPYLREQFGNPASRSHAYGWDAERAVEEAREQVAALVDADPREIVWTSGATESD
NLAIKGAANFYSGKGKHIITVKTEHKAVLDTTRELERQGFEVTYLDVKDDGLIDMDVFKQAIRPDTILVSVMLVNNEIGV
IQDVEQIGEICREKGIIFHCDAAQATGKVTIDLSTLKCDLMSFSAHKTYGPKGIGALYVRRKPRVRIEAQMHGGGHERGM
RSGTLAPHQIVGMGEAFRLAREEMATENERIRMLRDRLWNGLSSMEEVYLNGSMEHRVPHNLNVSFNFVEGESLIMAIKD
VAVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTVGRFTTEAEIDYTVELLKNKIGKLRDLSPLWEMFKDGVDLNSI
QWAAH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=400, Percent_Identity=59.5, Blast_Score=516, Evalue=1e-146,
Organism=Homo sapiens, GI156713448, Length=419, Percent_Identity=32.6968973747017, Blast_Score=201, Evalue=1e-51,
Organism=Escherichia coli, GI48994898, Length=402, Percent_Identity=75.3731343283582, Blast_Score=637, Evalue=0.0,
Organism=Escherichia coli, GI1789175, Length=229, Percent_Identity=29.2576419213974, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787970, Length=214, Percent_Identity=27.1028037383178, Blast_Score=89, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI25143064, Length=401, Percent_Identity=60.3491271820449, Blast_Score=514, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI17533177, Length=327, Percent_Identity=33.3333333333333, Blast_Score=146, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6319831, Length=399, Percent_Identity=58.6466165413534, Blast_Score=510, Evalue=1e-145,
Organism=Drosophila melanogaster, GI20129463, Length=400, Percent_Identity=57, Blast_Score=484, Evalue=1e-137,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 45306; Mature: 45175

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTPHFPIYMDYSATTPVDPRVADKMIPYLREQFGNPASRSHAYGWDAERAVEEAREQV
CCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
AALVDADPREIVWTSGATESDNLAIKGAANFYSGKGKHIITVKTEHKAVLDTTRELERQG
HHHHCCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCC
FEVTYLDVKDDGLIDMDVFKQAIRPDTILVSVMLVNNEIGVIQDVEQIGEICREKGIIFH
CEEEEEEECCCCEEEHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEE
CDAAQATGKVTIDLSTLKCDLMSFSAHKTYGPKGIGALYVRRKPRVRIEAQMHGGGHERG
ECCCCCCCEEEEEEEHEEEEEHHCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCHHCC
MRSGTLAPHQIVGMGEAFRLAREEMATENERIRMLRDRLWNGLSSMEEVYLNGSMEHRVP
CCCCCCCCHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCC
HNLNVSFNFVEGESLIMAIKDVAVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTVG
CCCCEEEEEECCCEEEEEEEHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEE
RFTTEAEIDYTVELLKNKIGKLRDLSPLWEMFKDGVDLNSIQWAAH
EEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEECC
>Mature Secondary Structure 
STTPHFPIYMDYSATTPVDPRVADKMIPYLREQFGNPASRSHAYGWDAERAVEEAREQV
CCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
AALVDADPREIVWTSGATESDNLAIKGAANFYSGKGKHIITVKTEHKAVLDTTRELERQG
HHHHCCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCC
FEVTYLDVKDDGLIDMDVFKQAIRPDTILVSVMLVNNEIGVIQDVEQIGEICREKGIIFH
CEEEEEEECCCCEEEHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEE
CDAAQATGKVTIDLSTLKCDLMSFSAHKTYGPKGIGALYVRRKPRVRIEAQMHGGGHERG
ECCCCCCCEEEEEEEHEEEEEHHCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCHHCC
MRSGTLAPHQIVGMGEAFRLAREEMATENERIRMLRDRLWNGLSSMEEVYLNGSMEHRVP
CCCCCCCCHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCC
HNLNVSFNFVEGESLIMAIKDVAVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTVG
CCCCEEEEEECCCEEEEEEEHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEE
RFTTEAEIDYTVELLKNKIGKLRDLSPLWEMFKDGVDLNSIQWAAH
EEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA