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The map label for this gene is nit [C]

Identifier: 94309937

GI number: 94309937

Start: 1078015

End: 1078836

Strand: Reverse

Name: nit [C]

Synonym: Rmet_0992

Alternate gene names: 94309937

Gene position: 1078836-1078015 (Counterclockwise)

Preceding gene: 94309938

Following gene: 94309936

Centisome position: 27.46

GC content: 66.79

Gene sequence:

>822_bases
ATGACTGCCACTGCCCCGATGCGCGTTGCCGCCATCCAGACCGTCACCGGCATCACGCTCGACGACAACCTCGCGCGTGC
CGATGCGCTGATCGCCGAGGCCGCGCGCGGCGGCGCCGAACTGGTGCTGCTGCCTGAGTACTTCTGCATGATGGGCCGCC
ACGAGACCGACAAGGTCGCCATCCGCGAACAGGATGGCGACGGTCCGGTGCAGAGCTTCCTGGCCGATGCCGCGCGGCGC
CATCGCGTCTGGCTCGTGGGCGGCACGCTGCCGATGTGGTGCAACGACGATGCGCGCGTCTACAACACGTCGCTCGCCTT
CGATCCGCATGGCCGCCGCGTGGCGCGCTACGACAAGATCCACCTGTTCGGCTTCACGAAGGGCACCGAGAGCTACGACG
AATCGCGCACGATCCTGGCCGGCAAGACACCGGTGGCCTTCGATGCGCCGTGCGGACGGGTGGCGATGTCGGTCTGCTAC
GACCTGCGCTTTCCGGAGCTCTATCGCGGACTGGCGGGCAAGAACGATGTCAGCCTGATCCTGATGCCGGCGGCTTTTAC
CTACACCACGGGCCAGGCTCACTGGGAGATCCTGCTGCGCGCCCGCGCGATCGAAAACCAGTGCTATGTGCTGGCCGCGG
CACAAGGCGGCAAGCACGAAAATGGCCGCCGCACCTGGGGTCACTCGATGCTGGTGGATCCGTGGGGCGAGTTGATGGGC
GTGTTGCCCGAGGGCGAAGGCATAGTGTCGGGTACGGTCGACCCGACCCGTCTTGCCGAAGTGCGTCAGAACCTGCCGGC
ACTGCGTCACCGCGTGCTGTAG

Upstream 100 bases:

>100_bases
AAGGTCACCAAAGTGGAAGACAATAAGACGCAACCGCCGATCCAGGAGCGTTCCGGCGCCTATCCGCGCTGAAGGCCCTG
CTCCAGAGAAGGAAGCCAAG

Downstream 100 bases:

>100_bases
GATTGACGGAAATTCACATTGCAGGCCACCGTGGCAGACCGCCACGGGGTATCGAAAAGGACAATCATGAACGCTGGCGA
TCTCGGAATCCGCAACCTGG

Product: Nitrilase

Products: carboxylate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MTATAPMRVAAIQTVTGITLDDNLARADALIAEAARGGAELVLLPEYFCMMGRHETDKVAIREQDGDGPVQSFLADAARR
HRVWLVGGTLPMWCNDDARVYNTSLAFDPHGRRVARYDKIHLFGFTKGTESYDESRTILAGKTPVAFDAPCGRVAMSVCY
DLRFPELYRGLAGKNDVSLILMPAAFTYTTGQAHWEILLRARAIENQCYVLAAAQGGKHENGRRTWGHSMLVDPWGELMG
VLPEGEGIVSGTVDPTRLAEVRQNLPALRHRVL

Sequences:

>Translated_273_residues
MTATAPMRVAAIQTVTGITLDDNLARADALIAEAARGGAELVLLPEYFCMMGRHETDKVAIREQDGDGPVQSFLADAARR
HRVWLVGGTLPMWCNDDARVYNTSLAFDPHGRRVARYDKIHLFGFTKGTESYDESRTILAGKTPVAFDAPCGRVAMSVCY
DLRFPELYRGLAGKNDVSLILMPAAFTYTTGQAHWEILLRARAIENQCYVLAAAQGGKHENGRRTWGHSMLVDPWGELMG
VLPEGEGIVSGTVDPTRLAEVRQNLPALRHRVL
>Mature_272_residues
TATAPMRVAAIQTVTGITLDDNLARADALIAEAARGGAELVLLPEYFCMMGRHETDKVAIREQDGDGPVQSFLADAARRH
RVWLVGGTLPMWCNDDARVYNTSLAFDPHGRRVARYDKIHLFGFTKGTESYDESRTILAGKTPVAFDAPCGRVAMSVCYD
LRFPELYRGLAGKNDVSLILMPAAFTYTTGQAHWEILLRARAIENQCYVLAAAQGGKHENGRRTWGHSMLVDPWGELMGV
LPEGEGIVSGTVDPTRLAEVRQNLPALRHRVL

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=266, Percent_Identity=36.4661654135338, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI297632350, Length=270, Percent_Identity=37.037037037037, Blast_Score=168, Evalue=6e-42,
Organism=Homo sapiens, GI5031947, Length=270, Percent_Identity=37.037037037037, Blast_Score=167, Evalue=7e-42,
Organism=Homo sapiens, GI297632348, Length=270, Percent_Identity=37.037037037037, Blast_Score=167, Evalue=7e-42,
Organism=Homo sapiens, GI297632346, Length=195, Percent_Identity=31.7948717948718, Blast_Score=86, Evalue=3e-17,
Organism=Escherichia coli, GI1786412, Length=233, Percent_Identity=29.6137339055794, Blast_Score=75, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17556280, Length=272, Percent_Identity=37.5, Blast_Score=171, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6323383, Length=275, Percent_Identity=33.0909090909091, Blast_Score=156, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6322335, Length=297, Percent_Identity=31.986531986532, Blast_Score=137, Evalue=2e-33,
Organism=Drosophila melanogaster, GI17933642, Length=257, Percent_Identity=36.5758754863813, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI21355835, Length=270, Percent_Identity=34.0740740740741, Blast_Score=148, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.5.1

Molecular weight: Translated: 30053; Mature: 29922

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATAPMRVAAIQTVTGITLDDNLARADALIAEAARGGAELVLLPEYFCMMGRHETDKVA
CCCCCCEEEEEEHHEECEEECCCHHHHHHHHHHHHCCCCEEEECHHHHHHCCCCCCCEEE
IREQDGDGPVQSFLADAARRHRVWLVGGTLPMWCNDDARVYNTSLAFDPHGRRVARYDKI
EEECCCCCHHHHHHHHHHHHCEEEEEECCCCEEECCCCEEEEEEEEECCCCCEEEEEEEE
HLFGFTKGTESYDESRTILAGKTPVAFDAPCGRVAMSVCYDLRFPELYRGLAGKNDVSLI
EEEEEECCCCCCCCCCEEEECCCCCEECCCHHHHHHHHHHHCCCHHHHHHCCCCCCEEEE
LMPAAFTYTTGQAHWEILLRARAIENQCYVLAAAQGGKHENGRRTWGHSMLVDPWGELMG
EEECEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEECCHHHHHC
VLPEGEGIVSGTVDPTRLAEVRQNLPALRHRVL
CCCCCCEEEECCCCHHHHHHHHHCCHHHHHHCC
>Mature Secondary Structure 
TATAPMRVAAIQTVTGITLDDNLARADALIAEAARGGAELVLLPEYFCMMGRHETDKVA
CCCCCEEEEEEHHEECEEECCCHHHHHHHHHHHHCCCCEEEECHHHHHHCCCCCCCEEE
IREQDGDGPVQSFLADAARRHRVWLVGGTLPMWCNDDARVYNTSLAFDPHGRRVARYDKI
EEECCCCCHHHHHHHHHHHHCEEEEEECCCCEEECCCCEEEEEEEEECCCCCEEEEEEEE
HLFGFTKGTESYDESRTILAGKTPVAFDAPCGRVAMSVCYDLRFPELYRGLAGKNDVSLI
EEEEEECCCCCCCCCCEEEECCCCCEECCCHHHHHHHHHHHCCCHHHHHHCCCCCCEEEE
LMPAAFTYTTGQAHWEILLRARAIENQCYVLAAAQGGKHENGRRTWGHSMLVDPWGELMG
EEECEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEECCHHHHHC
VLPEGEGIVSGTVDPTRLAEVRQNLPALRHRVL
CCCCCCEEEECCCCHHHHHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrile; H2O

Specific reaction: A nitrile + H2O = a carboxylate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]