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The map label for this gene is leuA [H]

Identifier: 94309863

GI number: 94309863

Start: 1002899

End: 1004440

Strand: Direct

Name: leuA [H]

Synonym: Rmet_0918

Alternate gene names: 94309863

Gene position: 1002899-1004440 (Clockwise)

Preceding gene: 94309862

Following gene: 94309865

Centisome position: 25.53

GC content: 61.54

Gene sequence:

>1542_bases
ATGTCTGACAAACTCATCATTTTCGACACCACCTTGCGTGACGGCGAGCAGTCGCCCGGCGCGTCCATGACCCGCGAAGA
GAAGATTCGCATTGCGCGTCAGCTTGAACGCCTGAAGGTCGACGTGATCGAGGCCGGTTTTGCGGCAAGCTCCAATGGTG
ACTTCGAGGCCATCCGCTCGATCGCCCAGGTGGTCAAGGATTCGACGATATGCTCGTTGGCCCGTGCCAATGACAAGGAC
ATCGCTCGTGCCGCCGAGGCGCTCAAGCCGGCAAACTCGTTCCGTATCCACACGTTCATCGCCACCTCGGCGCTGCACAT
GGAAAAGAAGCTGCGCATGACGCCGGACCAGGTTTATGAGCAGGCACGTCTGGCCGTGCGCTTCGCGCGCCAGTTCACGG
ACGACATCGAGTTCTCGCCGGAGGATGGCAGTCGCTCGGACATGGACTTCCTGTGCCGCGTGCTCGAAGGCGTGATCGCC
GAGGGCGCCACCACGATCAACCTGCCCGACACCGTGGGCTATGCCGTGCCAGAAGGCTATGCCGAGTTGATCCGCTCGGT
GCGCGAGCGCATTCCGAACTCGGACAAGGCCGTATGGTCGGTGCATTGCCACAATGACCTCGGCATGGCCGTGGCAAACT
CCCTGGCTGCTGTGAAGCTCGGCGGCGCGCGCCAGATCGAGTGCACGATCAACGGCCTTGGCGAACGCGCGGGTAACACC
AGCCTGGAAGAAGTAGTGATGGCCGTGAAGACGCGCCGTGACTATTTCAACATGGATGTGGGCATCGACACGACCCAGAT
CGTGCCGGCCTCAAAGCTTGTGTCGCAGATTACCGGCTTCGTCGTGCAGCCGAACAAGGCCGTGGTGGGCGCTAACGCGT
TTGCCCATGCATCCGGCATTCACCAGGATGGCGTGCTCAAGGCGCGCGATACCTACGAGATCATGCGCGCCGAGGATGTG
GGCTGGACGGCCAACAAGATCGTGCTTGGCAAGCTCTCGGGCCGCAATGCGTTCAAGCAGCGTCTGCAGGAGCTTGGCGT
CCAACTCGAAAGCGAAGCGGAGATCAATGCGGCATTTGCTCGCTTCAAGGAGCTCGCCGACCAGAAGGCCGAGATCTTCG
ACGAAGACATCATGGCGATCGTCTCCGATGAAGAGCAAGAGCACGCCAATGAGCACTATCGCTTCATCTCGCTGTCGCAG
CGCTCGGAAACGGGTGAGCGCCCCCATGCTCGCGTGGTGTTCAACATTGATGGCAGCGAGCAGACCGGCGAAGCCGAAGG
CAATGGTCCGGTGGACGCCACGCTGCACGCGATCGAAGGCAAGGTCAACAGCGGTGCCGAACTGGTGCTGTACTCGGTGA
ACGCGATCACCGCCGGCACGCAGGCCCAGGGTGAAGTCACGGTACGTCTGTCGAAGGCGGGCCGTATCGTCAATGGCGTG
GGTACCGACCCGGATATCGTCGCCGCATCGGCCAAGGCCTACCTGGCCGCGCTGAACAAGCTGCACGACAAGGCTGTGCA
GAAGATCAACCCGCAGATCTGA

Upstream 100 bases:

>100_bases
TGCACGCCGGGGCGTTTTGCATTCTGACGCGATTGTCCGGCAGCAAGATTGAGATCGGGACAGCACGGCACAACAGGGTA
CAGCCCAGGAGCTCCACAAA

Downstream 100 bases:

>100_bases
TCCCGAATCCTGCGATGCCGCTGGCGGGGCTCACAAGGCCCCGCCATCATGAAACGCCCGCCAGTCGCGTATCCCGCGAT
GGCGGGCGTTTTGTCGTCAG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 1; Alpha-isopropylmalate synthase 1 [H]

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MSDKLIIFDTTLRDGEQSPGASMTREEKIRIARQLERLKVDVIEAGFAASSNGDFEAIRSIAQVVKDSTICSLARANDKD
IARAAEALKPANSFRIHTFIATSALHMEKKLRMTPDQVYEQARLAVRFARQFTDDIEFSPEDGSRSDMDFLCRVLEGVIA
EGATTINLPDTVGYAVPEGYAELIRSVRERIPNSDKAVWSVHCHNDLGMAVANSLAAVKLGGARQIECTINGLGERAGNT
SLEEVVMAVKTRRDYFNMDVGIDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDV
GWTANKIVLGKLSGRNAFKQRLQELGVQLESEAEINAAFARFKELADQKAEIFDEDIMAIVSDEEQEHANEHYRFISLSQ
RSETGERPHARVVFNIDGSEQTGEAEGNGPVDATLHAIEGKVNSGAELVLYSVNAITAGTQAQGEVTVRLSKAGRIVNGV
GTDPDIVAASAKAYLAALNKLHDKAVQKINPQI

Sequences:

>Translated_513_residues
MSDKLIIFDTTLRDGEQSPGASMTREEKIRIARQLERLKVDVIEAGFAASSNGDFEAIRSIAQVVKDSTICSLARANDKD
IARAAEALKPANSFRIHTFIATSALHMEKKLRMTPDQVYEQARLAVRFARQFTDDIEFSPEDGSRSDMDFLCRVLEGVIA
EGATTINLPDTVGYAVPEGYAELIRSVRERIPNSDKAVWSVHCHNDLGMAVANSLAAVKLGGARQIECTINGLGERAGNT
SLEEVVMAVKTRRDYFNMDVGIDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDV
GWTANKIVLGKLSGRNAFKQRLQELGVQLESEAEINAAFARFKELADQKAEIFDEDIMAIVSDEEQEHANEHYRFISLSQ
RSETGERPHARVVFNIDGSEQTGEAEGNGPVDATLHAIEGKVNSGAELVLYSVNAITAGTQAQGEVTVRLSKAGRIVNGV
GTDPDIVAASAKAYLAALNKLHDKAVQKINPQI
>Mature_512_residues
SDKLIIFDTTLRDGEQSPGASMTREEKIRIARQLERLKVDVIEAGFAASSNGDFEAIRSIAQVVKDSTICSLARANDKDI
ARAAEALKPANSFRIHTFIATSALHMEKKLRMTPDQVYEQARLAVRFARQFTDDIEFSPEDGSRSDMDFLCRVLEGVIAE
GATTINLPDTVGYAVPEGYAELIRSVRERIPNSDKAVWSVHCHNDLGMAVANSLAAVKLGGARQIECTINGLGERAGNTS
LEEVVMAVKTRRDYFNMDVGIDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDVG
WTANKIVLGKLSGRNAFKQRLQELGVQLESEAEINAAFARFKELADQKAEIFDEDIMAIVSDEEQEHANEHYRFISLSQR
SETGERPHARVVFNIDGSEQTGEAEGNGPVDATLHAIEGKVNSGAELVLYSVNAITAGTQAQGEVTVRLSKAGRIVNGVG
TDPDIVAASAKAYLAALNKLHDKAVQKINPQI

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=501, Percent_Identity=49.3013972055888, Blast_Score=458, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6324682, Length=538, Percent_Identity=26.7657992565056, Blast_Score=144, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6324225, Length=544, Percent_Identity=26.8382352941176, Blast_Score=143, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6320071, Length=386, Percent_Identity=29.0155440414508, Blast_Score=134, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6320019, Length=382, Percent_Identity=28.7958115183246, Blast_Score=131, Evalue=2e-31,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 55793; Mature: 55662

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKLIIFDTTLRDGEQSPGASMTREEKIRIARQLERLKVDVIEAGFAASSNGDFEAIRS
CCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
IAQVVKDSTICSLARANDKDIARAAEALKPANSFRIHTFIATSALHMEKKLRMTPDQVYE
HHHHHHCHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHCCCHHHHHH
QARLAVRFARQFTDDIEFSPEDGSRSDMDFLCRVLEGVIAEGATTINLPDTVGYAVPEGY
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHH
AELIRSVRERIPNSDKAVWSVHCHNDLGMAVANSLAAVKLGGARQIECTINGLGERAGNT
HHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHCHHEEEECCCEEEEEEEECCCCCCCCC
SLEEVVMAVKTRRDYFNMDVGIDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGI
CHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHCEEECCCCEEECCCHHHHHCCC
HQDGVLKARDTYEIMRAEDVGWTANKIVLGKLSGRNAFKQRLQELGVQLESEAEINAAFA
CCCCCEECCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCEECCCHHHHHHHH
RFKELADQKAEIFDEDIMAIVSDEEQEHANEHYRFISLSQRSETGERPHARVVFNIDGSE
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCEEEEEECCCCCCCCCCCEEEEEEECCCC
QTGEAEGNGPVDATLHAIEGKVNSGAELVLYSVNAITAGTQAQGEVTVRLSKAGRIVNGV
CCCCCCCCCCHHHEEEEECCCCCCCCEEEEEEEEEEECCCCCCCEEEEEEECCCCEEECC
GTDPDIVAASAKAYLAALNKLHDKAVQKINPQI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SDKLIIFDTTLRDGEQSPGASMTREEKIRIARQLERLKVDVIEAGFAASSNGDFEAIRS
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
IAQVVKDSTICSLARANDKDIARAAEALKPANSFRIHTFIATSALHMEKKLRMTPDQVYE
HHHHHHCHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHCCCHHHHHH
QARLAVRFARQFTDDIEFSPEDGSRSDMDFLCRVLEGVIAEGATTINLPDTVGYAVPEGY
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHH
AELIRSVRERIPNSDKAVWSVHCHNDLGMAVANSLAAVKLGGARQIECTINGLGERAGNT
HHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHCHHEEEECCCEEEEEEEECCCCCCCCC
SLEEVVMAVKTRRDYFNMDVGIDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGI
CHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHCEEECCCCEEECCCHHHHHCCC
HQDGVLKARDTYEIMRAEDVGWTANKIVLGKLSGRNAFKQRLQELGVQLESEAEINAAFA
CCCCCEECCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCEECCCHHHHHHHH
RFKELADQKAEIFDEDIMAIVSDEEQEHANEHYRFISLSQRSETGERPHARVVFNIDGSE
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCEEEEEECCCCCCCCCCCEEEEEEECCCC
QTGEAEGNGPVDATLHAIEGKVNSGAELVLYSVNAITAGTQAQGEVTVRLSKAGRIVNGV
CCCCCCCCCCHHHEEEEECCCCCCCCEEEEEEEEEEECCCCCCCEEEEEEECCCCEEECC
GTDPDIVAASAKAYLAALNKLHDKAVQKINPQI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]