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The map label for this gene is ilvI [H]

Identifier: 94309856

GI number: 94309856

Start: 996807

End: 998570

Strand: Direct

Name: ilvI [H]

Synonym: Rmet_0911

Alternate gene names: 94309856

Gene position: 996807-998570 (Clockwise)

Preceding gene: 335055510

Following gene: 94309857

Centisome position: 25.38

GC content: 60.2

Gene sequence:

>1764_bases
ATGAACATGCCCAGCGCGGAATTTTCCCACGCCGATAGCAATTCATCCTCCGCTCGCGAAATGATCGGAGCGGAAATCCT
CGTCAACGCACTTGCCGAAGAAGGCGTCGAATACGTCTGGGGTTATCCCGGCGGCGCAGTGCTCTATATCTACGACGAGC
TCCACAAGCAAACCAAATTCGAACATATCCTGGTGCGCCACGAGCAGGCCGCCGTCCATGCCGCTGACGGGTACGCCCGT
GCGACCGGCAAGGTGGGCGTTGCGCTGGTGACCTCGGGTCCGGGCGTGACCAATGCCGTGACCGGCATCGCTACCGCCTA
TCTGGATTCGATTCCGATGGTGGTGATCACCGGCAACGTGCCGACTCACGCGATCGGTCAGGATGCATTCCAGGAATGCG
ACACCGTTGGCATCACGCGCCCGATCGTCAAGCACAACTTCCTCGTGAAGGACGTGCGTGACCTGGCCGCCACGATCAAG
AAGGCGTTCTTCATTGCCGCCACCGGCCGTCCCGGCCCTGTGGTGGTCGATATCCCGAAGGACGTGTCGCGCAACAGCTG
CAAGTACGAGTATCCGAAGTCGATTGACATGCGCTCGTACAGCCCGGTGAACAAGGGCCATTCGGGTCAGATCCGCAAGG
CCGTGGCGTTGCTGCAATCGGCCGAGCGCCCGTACATCTATTCGGGCGGTGGCGTGGTGCTGGCCAACGCAAGCGAAGAG
CTGCGCGAGCTGGCCGCGCTCACCGGTCATCCGGTCACCAATACGCTGATGGGCCTGGGCGCGTTCCCCGGCACGAGCAA
GCAGTTCGTTGGCATGCTCGGCATGCACGGCACGTACGAGGCCAACATGGCCATGCAGAACTGCGACGTGCTGATTGCCA
TCGGCGCCCGATTCGATGACCGCGTGATCGGCAACCCGTCGCACTTCACTGCGCAGGCCCGCAAGATCATCCACATCGAC
ATCGACCCGTCGTCGATCTCGAAGCGCGTCAAGGTGGACATTCCCATCGTTGGCAACGTCAAGGACGTGCTGCAGGAACT
GATTTCCCAGATCAAGACGAGCGATATCAAGCCCAAGCGCGAGGCTCTGGCCAAGTGGTGGGAACAGATCGAGAAGTGGC
GCTCGGTGGACTGCCTCAAGTACGACCGCAGTTCCGAGATCATCAAGCCGCAGTACGTGGTGGAAAAGATCTGGGAACTG
ACGCACGGCGACGCATTCATCTGCTCCGACGTGGGCCAGCACCAGATGTGGGCCGCGCAGTTCTACAAGTTCAACGAGCC
GCGTCGCTGGATCAATTCCGGTGGCCTCGGCACGATGGGCGTGGGTCTGCCGTACGCAATGGGCATCAAGAAGGCATTCC
CGGACAAGGAAGTCGTCACCATCACTGGTGAAGGCTCGATCCAGATGTGTATCCAGGAACTGTCGACCTGCCTGCAGTAC
GACACCCCGGTCAAGATCTGCTCGCTGAACAACGGATATCTGGGCATGGTGCGCCAGTGGCAGGAAATCGAGTACGACAA
TCGTTACTCGCATTCCTACATGCAGGCATTGCCCGACTTCGTCAAACTCGCCGAAGCCTATGGCCACGTGGGGATGCGCA
TCGAGAAGAGCTCCGATGTGGAACCCGCGTTGCGCGAGGCTTTCCGACTCAAGGACCGTACCGTGTTCCTGGACTTCCAG
ACCGACCCCACTGAAAACGTGTGGCCAATGGTTCAGGCTGGCAAGGGTATCTCCGAAATGCTGCTTGGCGCGGAGGACCT
GTAA

Upstream 100 bases:

>100_bases
AGGCGGTCTTCTCATGCAGACGCGCACCGCTTGAACCCGAGCCAAAAGCCCGGCAGAGAGCATCCAAACAATTTTTTGCC
GAAAATTGCAAAGGACTGAA

Downstream 100 bases:

>100_bases
TGCGACACATCATCTCGGTCCTGCTGGAGAATGAACCCGGCGCGCTGTCGCGCGTGGTGGGCCTGTTTTCGGCACGTGGA
TACAACATCGAGACGCTGAC

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MNMPSAEFSHADSNSSSAREMIGAEILVNALAEEGVEYVWGYPGGAVLYIYDELHKQTKFEHILVRHEQAAVHAADGYAR
ATGKVGVALVTSGPGVTNAVTGIATAYLDSIPMVVITGNVPTHAIGQDAFQECDTVGITRPIVKHNFLVKDVRDLAATIK
KAFFIAATGRPGPVVVDIPKDVSRNSCKYEYPKSIDMRSYSPVNKGHSGQIRKAVALLQSAERPYIYSGGGVVLANASEE
LRELAALTGHPVTNTLMGLGAFPGTSKQFVGMLGMHGTYEANMAMQNCDVLIAIGARFDDRVIGNPSHFTAQARKIIHID
IDPSSISKRVKVDIPIVGNVKDVLQELISQIKTSDIKPKREALAKWWEQIEKWRSVDCLKYDRSSEIIKPQYVVEKIWEL
THGDAFICSDVGQHQMWAAQFYKFNEPRRWINSGGLGTMGVGLPYAMGIKKAFPDKEVVTITGEGSIQMCIQELSTCLQY
DTPVKICSLNNGYLGMVRQWQEIEYDNRYSHSYMQALPDFVKLAEAYGHVGMRIEKSSDVEPALREAFRLKDRTVFLDFQ
TDPTENVWPMVQAGKGISEMLLGAEDL

Sequences:

>Translated_587_residues
MNMPSAEFSHADSNSSSAREMIGAEILVNALAEEGVEYVWGYPGGAVLYIYDELHKQTKFEHILVRHEQAAVHAADGYAR
ATGKVGVALVTSGPGVTNAVTGIATAYLDSIPMVVITGNVPTHAIGQDAFQECDTVGITRPIVKHNFLVKDVRDLAATIK
KAFFIAATGRPGPVVVDIPKDVSRNSCKYEYPKSIDMRSYSPVNKGHSGQIRKAVALLQSAERPYIYSGGGVVLANASEE
LRELAALTGHPVTNTLMGLGAFPGTSKQFVGMLGMHGTYEANMAMQNCDVLIAIGARFDDRVIGNPSHFTAQARKIIHID
IDPSSISKRVKVDIPIVGNVKDVLQELISQIKTSDIKPKREALAKWWEQIEKWRSVDCLKYDRSSEIIKPQYVVEKIWEL
THGDAFICSDVGQHQMWAAQFYKFNEPRRWINSGGLGTMGVGLPYAMGIKKAFPDKEVVTITGEGSIQMCIQELSTCLQY
DTPVKICSLNNGYLGMVRQWQEIEYDNRYSHSYMQALPDFVKLAEAYGHVGMRIEKSSDVEPALREAFRLKDRTVFLDFQ
TDPTENVWPMVQAGKGISEMLLGAEDL
>Mature_587_residues
MNMPSAEFSHADSNSSSAREMIGAEILVNALAEEGVEYVWGYPGGAVLYIYDELHKQTKFEHILVRHEQAAVHAADGYAR
ATGKVGVALVTSGPGVTNAVTGIATAYLDSIPMVVITGNVPTHAIGQDAFQECDTVGITRPIVKHNFLVKDVRDLAATIK
KAFFIAATGRPGPVVVDIPKDVSRNSCKYEYPKSIDMRSYSPVNKGHSGQIRKAVALLQSAERPYIYSGGGVVLANASEE
LRELAALTGHPVTNTLMGLGAFPGTSKQFVGMLGMHGTYEANMAMQNCDVLIAIGARFDDRVIGNPSHFTAQARKIIHID
IDPSSISKRVKVDIPIVGNVKDVLQELISQIKTSDIKPKREALAKWWEQIEKWRSVDCLKYDRSSEIIKPQYVVEKIWEL
THGDAFICSDVGQHQMWAAQFYKFNEPRRWINSGGLGTMGVGLPYAMGIKKAFPDKEVVTITGEGSIQMCIQELSTCLQY
DTPVKICSLNNGYLGMVRQWQEIEYDNRYSHSYMQALPDFVKLAEAYGHVGMRIEKSSDVEPALREAFRLKDRTVFLDFQ
TDPTENVWPMVQAGKGISEMLLGAEDL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=582, Percent_Identity=25.7731958762887, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI21361361, Length=567, Percent_Identity=24.5149911816578, Blast_Score=145, Evalue=1e-34,
Organism=Escherichia coli, GI87081685, Length=568, Percent_Identity=57.0422535211268, Blast_Score=685, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=574, Percent_Identity=40.2439024390244, Blast_Score=411, Evalue=1e-116,
Organism=Escherichia coli, GI1786717, Length=531, Percent_Identity=34.4632768361582, Blast_Score=296, Evalue=3e-81,
Organism=Escherichia coli, GI1787096, Length=568, Percent_Identity=29.4014084507042, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1788716, Length=554, Percent_Identity=24.3682310469314, Blast_Score=147, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17531299, Length=567, Percent_Identity=27.1604938271605, Blast_Score=163, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17531301, Length=567, Percent_Identity=27.1604938271605, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17542570, Length=511, Percent_Identity=25.2446183953033, Blast_Score=145, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6323755, Length=582, Percent_Identity=43.4707903780069, Blast_Score=452, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6320816, Length=501, Percent_Identity=24.3512974051896, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323163, Length=522, Percent_Identity=23.5632183908046, Blast_Score=81, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6320123, Length=505, Percent_Identity=20.990099009901, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922626, Length=571, Percent_Identity=26.0945709281961, Blast_Score=178, Evalue=1e-44,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64785; Mature: 64785

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMPSAEFSHADSNSSSAREMIGAEILVNALAEEGVEYVWGYPGGAVLYIYDELHKQTKF
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEHHHHHHHHH
EHILVRHEQAAVHAADGYARATGKVGVALVTSGPGVTNAVTGIATAYLDSIPMVVITGNV
HHHHHCCHHHHEECCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCC
PTHAIGQDAFQECDTVGITRPIVKHNFLVKDVRDLAATIKKAFFIAATGRPGPVVVDIPK
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCC
DVSRNSCKYEYPKSIDMRSYSPVNKGHSGQIRKAVALLQSAERPYIYSGGGVVLANASEE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCEEEECCHHH
LRELAALTGHPVTNTLMGLGAFPGTSKQFVGMLGMHGTYEANMAMQNCDVLIAIGARFDD
HHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCEECCCHHCCCCEEEEECCCCCC
RVIGNPSHFTAQARKIIHIDIDPSSISKRVKVDIPIVGNVKDVLQELISQIKTSDIKPKR
CCCCCCCHHHHCCCEEEEEEECHHHHCCEEEEEEEEECCHHHHHHHHHHHHHCCCCCHHH
EALAKWWEQIEKWRSVDCLKYDRSSEIIKPQYVVEKIWELTHGDAFICSDVGQHQMWAAQ
HHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHH
FYKFNEPRRWINSGGLGTMGVGLPYAMGIKKAFPDKEVVTITGEGSIQMCIQELSTCLQY
HHHCCCCHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCC
DTPVKICSLNNGYLGMVRQWQEIEYDNRYSHSYMQALPDFVKLAEAYGHVGMRIEKSSDV
CCCEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
EPALREAFRLKDRTVFLDFQTDPTENVWPMVQAGKGISEMLLGAEDL
CHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHCCCCCC
>Mature Secondary Structure
MNMPSAEFSHADSNSSSAREMIGAEILVNALAEEGVEYVWGYPGGAVLYIYDELHKQTKF
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEHHHHHHHHH
EHILVRHEQAAVHAADGYARATGKVGVALVTSGPGVTNAVTGIATAYLDSIPMVVITGNV
HHHHHCCHHHHEECCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCC
PTHAIGQDAFQECDTVGITRPIVKHNFLVKDVRDLAATIKKAFFIAATGRPGPVVVDIPK
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCC
DVSRNSCKYEYPKSIDMRSYSPVNKGHSGQIRKAVALLQSAERPYIYSGGGVVLANASEE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCEEEECCHHH
LRELAALTGHPVTNTLMGLGAFPGTSKQFVGMLGMHGTYEANMAMQNCDVLIAIGARFDD
HHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCEECCCHHCCCCEEEEECCCCCC
RVIGNPSHFTAQARKIIHIDIDPSSISKRVKVDIPIVGNVKDVLQELISQIKTSDIKPKR
CCCCCCCHHHHCCCEEEEEEECHHHHCCEEEEEEEEECCHHHHHHHHHHHHHCCCCCHHH
EALAKWWEQIEKWRSVDCLKYDRSSEIIKPQYVVEKIWELTHGDAFICSDVGQHQMWAAQ
HHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHH
FYKFNEPRRWINSGGLGTMGVGLPYAMGIKKAFPDKEVVTITGEGSIQMCIQELSTCLQY
HHHCCCCHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCC
DTPVKICSLNNGYLGMVRQWQEIEYDNRYSHSYMQALPDFVKLAEAYGHVGMRIEKSSDV
CCCEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
EPALREAFRLKDRTVFLDFQTDPTENVWPMVQAGKGISEMLLGAEDL
CHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]