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The map label for this gene is maeB [H]

Identifier: 94309823

GI number: 94309823

Start: 960937

End: 963258

Strand: Direct

Name: maeB [H]

Synonym: Rmet_0878

Alternate gene names: 94309823

Gene position: 960937-963258 (Clockwise)

Preceding gene: 94309822

Following gene: 94309830

Centisome position: 24.46

GC content: 64.94

Gene sequence:

>2322_bases
ATGAGCAGCAAGACCACCGGTGATGCACCGCAACATGCCCCCAATAGTCCCGAAGCGCAACTGCGCCTGGCTGCGCTGGA
ATACCACCGCAGCCCGACCAAGGGCAAGATCCAGGTAACGGCCACCAAGGCCCTGTCCAATCAGCGTGACCTGTCGCTGG
CCTACTCCCCGGGCGTGGCCTACGCCTGCGAAGAAATCCACAAGGACCCCGCGATGGCCGCCGAGTACACCTCGCGCGCC
AACCTCGTGGCCGTGGTGACCAACGGTACCGCCGTGTTGGGCTTGGGCGACATCGGTCCGCTGGCCGGCAAGCCCGTGAT
GGAAGGCAAGGGCTGCCTGTTCAAGAAATTCGCCGGCATCGACGTGTTCGACATCGAACTCGATGCACGCGACCCGGACA
AGATCGTCGAGATCGTAGCGGCGCTGGAGCCGACGCTTGGTGGCGTGAACCTCGAAGACATCAAGGCTCCCGAGTGCTTC
TACATCGAGCAGAAGCTGCGCGAGCGCATGAACATCCCCGTCTTCCACGACGATCAGCACGGCACGGCGATCATCTCGAC
CGCGGCGCTGCTGAACGGCCTGAAGGTGGTAGGCAAGGACATCGCGAAGGTCAAGCTGGCCGTCTCGGGCGCGGGCGCCG
CCGCAATCGCGTGCCTGGATACGATGGTCAGCCTGGGCGTGAACCGCGAGAACATCTACGTGGTCGACTCGAAGGGCGTG
ATCTTCCAGGGCCGCGACGCGAACATGGAGGCCAACAAGGCTCGCTACGCGCAGGACACGTCGGCCCGCACGCTGGCCGA
CATCGTCAAGGATGCGGACGTGTTCCTGGGTTGCTCGACCGCGGGCGTGCTGACCGGTGACATGGTCAAGACCATGGCCG
ACCGTCCGATCATCCTGGCGCTGGCCAACCCGGAGCCGGAAATCCGCCCGGAAGTGGCCAAGGCCGCGCGTCCGGACTGC
ATCATCGCCACGGGCCGTTCGGACTATCCGAACCAGGTCAACAACGTGCTCTGCTTCCCGTACATCTTCCGCGGCGCGCT
CGATTGCGGCGCCACGAAGATCACCGAAGCGATGAAGCTGGCCTGCGTGAAGGCGATCGCCGAGCTGGCCGAAGCCGAAC
TGAACGATGCCGTGGCCGCAGCCTACGGTGGTCGTGAACTGAAGTTCGGCCCGGACTACATCATCCCGACGCCGTTCGAC
CAGCGCCTGATCGAGAAGATTGCACCGGCCGTTGCCAAGGCCGCCGAGGAATCCGGCGTGGCCACGCGCCCGATCAAGGA
CCTTGAAGCCTACCGCCAGCAACTGACCAGCTACGTGTACCACACCGGCATGATCATGAAGCCGGTGTTCACGGCCGCCA
AGGCCGCGCCGAAACGCGTCGCCTACGCCGAAGGTGAAGAAGAGCGTGTGCTGCGTGCGGTGCAGGGCGTGGTGGACGAA
GGTCTGGCCCGTCCGATCCTGATCGGTCGCCCGCACGTGATCCAGATGCGTATCGACAAGGCGGGCCTGCGTCTGCGCCC
GGGCGTCGATTTCGAACTGATCAACCCCGAGGATGATCCGCGTTATCGCGCCTATCACGAGGAGTACCACGCGCTGCGCG
GTCGCGACGGCGTGACGCCGGACATGGCCAAGGTGGCGCTGCGTCGTTCGAATACGCTGATCGGCACGATGCTGATGCAC
ATGGGTGATGCCGATGCACTGTTGTGCGGCACGGTGGGCCGGTTCGAGGCGCATCTGGAACACGTGCGCGACGTGATCGG
CCTGGCGCCGGGCGCCAAGGTGTTCGCGGCCATGAACGCGCTGATGCTCGAAAAGTACACGCTGTTCATCACGGATACGT
TCGTCAACGATGATCCGAGCGCCGAGGAACTGGCTGCCATCACGCAGCTTGCAGCCGAGGAAATCGCACGCTTCGGCCTG
CACCCGAAGGTTGCGATGATGTCGCACTCGATGTTCGGTTCGTCGAACCGCCCGTCGGCGCGCAAGATGCGCGACGCGGC
CGAGATCCTGGCCCGCGTGGCACCGCAGCTCGAAGTGGAAGGCGAGATGCAGGGCGATGCGGCACTGGTCGAAGACGTGC
GCCGTCATTTCCTGCCGGGCACCAAGCTGGCCGGCAGCGCCAATCTGCTGGTGATGCCGACGCTGGACGCCGCCAACATC
GCGTTCAATCTGCTCAAGATCACGGGCGGCCAGGGCGTGACGGTGGGTCCGATCCTGCTGGGCGCAGCCAAGCCGGTGCA
CATCCTGAATCCGCAGGCGACCACGCGCCGTATCGTCAACATGACGGCCGTGGCCGTGGCGGAGTGCAACGCCACCCGCT
GA

Upstream 100 bases:

>100_bases
AAAATCTTCCAATCGGGCGTGCCAAAGCCCTGCCGCCGCGTGGCGGCTGGGCTAGAATGGCTCCTCGATTTTTTCCCGCC
TTTACCCGGACGAGCAGAGA

Downstream 100 bases:

>100_bases
TGTTCACGATCTGAATATCTGCCGGAAGCAAAAAGAAACAGGGCCGCGAAAGCGGCCCTGTTTCATTGTCGGTGCGCTGG
CACGTCTGCCGGAGTGTCAG

Product: malic enzyme

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 773; Mature: 772

Protein sequence:

>773_residues
MSSKTTGDAPQHAPNSPEAQLRLAALEYHRSPTKGKIQVTATKALSNQRDLSLAYSPGVAYACEEIHKDPAMAAEYTSRA
NLVAVVTNGTAVLGLGDIGPLAGKPVMEGKGCLFKKFAGIDVFDIELDARDPDKIVEIVAALEPTLGGVNLEDIKAPECF
YIEQKLRERMNIPVFHDDQHGTAIISTAALLNGLKVVGKDIAKVKLAVSGAGAAAIACLDTMVSLGVNRENIYVVDSKGV
IFQGRDANMEANKARYAQDTSARTLADIVKDADVFLGCSTAGVLTGDMVKTMADRPIILALANPEPEIRPEVAKAARPDC
IIATGRSDYPNQVNNVLCFPYIFRGALDCGATKITEAMKLACVKAIAELAEAELNDAVAAAYGGRELKFGPDYIIPTPFD
QRLIEKIAPAVAKAAEESGVATRPIKDLEAYRQQLTSYVYHTGMIMKPVFTAAKAAPKRVAYAEGEEERVLRAVQGVVDE
GLARPILIGRPHVIQMRIDKAGLRLRPGVDFELINPEDDPRYRAYHEEYHALRGRDGVTPDMAKVALRRSNTLIGTMLMH
MGDADALLCGTVGRFEAHLEHVRDVIGLAPGAKVFAAMNALMLEKYTLFITDTFVNDDPSAEELAAITQLAAEEIARFGL
HPKVAMMSHSMFGSSNRPSARKMRDAAEILARVAPQLEVEGEMQGDAALVEDVRRHFLPGTKLAGSANLLVMPTLDAANI
AFNLLKITGGQGVTVGPILLGAAKPVHILNPQATTRRIVNMTAVAVAECNATR

Sequences:

>Translated_773_residues
MSSKTTGDAPQHAPNSPEAQLRLAALEYHRSPTKGKIQVTATKALSNQRDLSLAYSPGVAYACEEIHKDPAMAAEYTSRA
NLVAVVTNGTAVLGLGDIGPLAGKPVMEGKGCLFKKFAGIDVFDIELDARDPDKIVEIVAALEPTLGGVNLEDIKAPECF
YIEQKLRERMNIPVFHDDQHGTAIISTAALLNGLKVVGKDIAKVKLAVSGAGAAAIACLDTMVSLGVNRENIYVVDSKGV
IFQGRDANMEANKARYAQDTSARTLADIVKDADVFLGCSTAGVLTGDMVKTMADRPIILALANPEPEIRPEVAKAARPDC
IIATGRSDYPNQVNNVLCFPYIFRGALDCGATKITEAMKLACVKAIAELAEAELNDAVAAAYGGRELKFGPDYIIPTPFD
QRLIEKIAPAVAKAAEESGVATRPIKDLEAYRQQLTSYVYHTGMIMKPVFTAAKAAPKRVAYAEGEEERVLRAVQGVVDE
GLARPILIGRPHVIQMRIDKAGLRLRPGVDFELINPEDDPRYRAYHEEYHALRGRDGVTPDMAKVALRRSNTLIGTMLMH
MGDADALLCGTVGRFEAHLEHVRDVIGLAPGAKVFAAMNALMLEKYTLFITDTFVNDDPSAEELAAITQLAAEEIARFGL
HPKVAMMSHSMFGSSNRPSARKMRDAAEILARVAPQLEVEGEMQGDAALVEDVRRHFLPGTKLAGSANLLVMPTLDAANI
AFNLLKITGGQGVTVGPILLGAAKPVHILNPQATTRRIVNMTAVAVAECNATR
>Mature_772_residues
SSKTTGDAPQHAPNSPEAQLRLAALEYHRSPTKGKIQVTATKALSNQRDLSLAYSPGVAYACEEIHKDPAMAAEYTSRAN
LVAVVTNGTAVLGLGDIGPLAGKPVMEGKGCLFKKFAGIDVFDIELDARDPDKIVEIVAALEPTLGGVNLEDIKAPECFY
IEQKLRERMNIPVFHDDQHGTAIISTAALLNGLKVVGKDIAKVKLAVSGAGAAAIACLDTMVSLGVNRENIYVVDSKGVI
FQGRDANMEANKARYAQDTSARTLADIVKDADVFLGCSTAGVLTGDMVKTMADRPIILALANPEPEIRPEVAKAARPDCI
IATGRSDYPNQVNNVLCFPYIFRGALDCGATKITEAMKLACVKAIAELAEAELNDAVAAAYGGRELKFGPDYIIPTPFDQ
RLIEKIAPAVAKAAEESGVATRPIKDLEAYRQQLTSYVYHTGMIMKPVFTAAKAAPKRVAYAEGEEERVLRAVQGVVDEG
LARPILIGRPHVIQMRIDKAGLRLRPGVDFELINPEDDPRYRAYHEEYHALRGRDGVTPDMAKVALRRSNTLIGTMLMHM
GDADALLCGTVGRFEAHLEHVRDVIGLAPGAKVFAAMNALMLEKYTLFITDTFVNDDPSAEELAAITQLAAEEIARFGLH
PKVAMMSHSMFGSSNRPSARKMRDAAEILARVAPQLEVEGEMQGDAALVEDVRRHFLPGTKLAGSANLLVMPTLDAANIA
FNLLKITGGQGVTVGPILLGAAKPVHILNPQATTRRIVNMTAVAVAECNATR

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4505145, Length=404, Percent_Identity=25.990099009901, Blast_Score=95, Evalue=3e-19,
Organism=Homo sapiens, GI62420882, Length=409, Percent_Identity=25.6723716381418, Blast_Score=94, Evalue=6e-19,
Organism=Homo sapiens, GI62420880, Length=409, Percent_Identity=25.6723716381418, Blast_Score=94, Evalue=6e-19,
Organism=Homo sapiens, GI239049447, Length=409, Percent_Identity=25.6723716381418, Blast_Score=94, Evalue=6e-19,
Organism=Homo sapiens, GI4505143, Length=398, Percent_Identity=25.1256281407035, Blast_Score=94, Evalue=6e-19,
Organism=Homo sapiens, GI270265879, Length=369, Percent_Identity=26.5582655826558, Blast_Score=86, Evalue=2e-16,
Organism=Escherichia coli, GI1788806, Length=750, Percent_Identity=63.3333333333333, Blast_Score=977, Evalue=0.0,
Organism=Escherichia coli, GI1788635, Length=335, Percent_Identity=28.6567164179104, Blast_Score=136, Evalue=6e-33,
Organism=Escherichia coli, GI1788800, Length=328, Percent_Identity=31.4024390243902, Blast_Score=136, Evalue=6e-33,
Organism=Escherichia coli, GI87081919, Length=459, Percent_Identity=23.0936819172113, Blast_Score=93, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17537199, Length=404, Percent_Identity=25.990099009901, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6322823, Length=353, Percent_Identity=28.328611898017, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21356279, Length=503, Percent_Identity=27.037773359841, Blast_Score=105, Evalue=2e-22,
Organism=Drosophila melanogaster, GI281362674, Length=474, Percent_Identity=27.2151898734177, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI281362672, Length=480, Percent_Identity=27.0833333333333, Blast_Score=104, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24646388, Length=400, Percent_Identity=28.25, Blast_Score=95, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24646386, Length=400, Percent_Identity=28.25, Blast_Score=95, Evalue=2e-19,
Organism=Drosophila melanogaster, GI78707242, Length=431, Percent_Identity=27.3781902552204, Blast_Score=80, Evalue=7e-15,
Organism=Drosophila melanogaster, GI78707238, Length=431, Percent_Identity=27.3781902552204, Blast_Score=80, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 83026; Mature: 82895

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKTTGDAPQHAPNSPEAQLRLAALEYHRSPTKGKIQVTATKALSNQRDLSLAYSPGVA
CCCCCCCCCCCCCCCCCHHHEEEEHHHHCCCCCCCEEEEEEEHHHCCCCCEEEEECCCHH
YACEEIHKDPAMAAEYTSRANLVAVVTNGTAVLGLGDIGPLAGKPVMEGKGCLFKKFAGI
HHHHHHCCCCCHHHHHCCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCCCHHHHHCCC
DVFDIELDARDPDKIVEIVAALEPTLGGVNLEDIKAPECFYIEQKLRERMNIPVFHDDQH
EEEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCEEECCCC
GTAIISTAALLNGLKVVGKDIAKVKLAVSGAGAAAIACLDTMVSLGVNRENIYVVDSKGV
CCHHHHHHHHHHHHHHHCCCHHEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCE
IFQGRDANMEANKARYAQDTSARTLADIVKDADVFLGCSTAGVLTGDMVKTMADRPIILA
EEECCCCCCCCCHHHHCCCCHHHHHHHHHHCCHHEEECCCCCCHHHHHHHHHCCCCEEEE
LANPEPEIRPEVAKAARPDCIIATGRSDYPNQVNNVLCFPYIFRGALDCGATKITEAMKL
ECCCCCCCCHHHHHCCCCCEEEECCCCCCCHHHCCEEEHHHHHHHHHHCCHHHHHHHHHH
ACVKAIAELAEAELNDAVAAAYGGRELKFGPDYIIPTPFDQRLIEKIAPAVAKAAEESGV
HHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
ATRPIKDLEAYRQQLTSYVYHTGMIMKPVFTAAKAAPKRVAYAEGEEERVLRAVQGVVDE
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHH
GLARPILIGRPHVIQMRIDKAGLRLRPGVDFELINPEDDPRYRAYHEEYHALRGRDGVTP
HCCCCEEECCCEEEEEEECCCCCEECCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCH
DMAKVALRRSNTLIGTMLMHMGDADALLCGTVGRFEAHLEHVRDVIGLAPGAKVFAAMNA
HHHHHHHHHCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LMLEKYTLFITDTFVNDDPSAEELAAITQLAAEEIARFGLHPKVAMMSHSMFGSSNRPSA
HHHHHEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHH
RKMRDAAEILARVAPQLEVEGEMQGDAALVEDVRRHFLPGTKLAGSANLLVMPTLDAANI
HHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHH
AFNLLKITGGQGVTVGPILLGAAKPVHILNPQATTRRIVNMTAVAVAECNATR
EEEEEEEECCCCCEEHHEEECCCCCEEEECCCHHHHHHHHHHHHEEECCCCCC
>Mature Secondary Structure 
SSKTTGDAPQHAPNSPEAQLRLAALEYHRSPTKGKIQVTATKALSNQRDLSLAYSPGVA
CCCCCCCCCCCCCCCCHHHEEEEHHHHCCCCCCCEEEEEEEHHHCCCCCEEEEECCCHH
YACEEIHKDPAMAAEYTSRANLVAVVTNGTAVLGLGDIGPLAGKPVMEGKGCLFKKFAGI
HHHHHHCCCCCHHHHHCCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCCCHHHHHCCC
DVFDIELDARDPDKIVEIVAALEPTLGGVNLEDIKAPECFYIEQKLRERMNIPVFHDDQH
EEEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCEEECCCC
GTAIISTAALLNGLKVVGKDIAKVKLAVSGAGAAAIACLDTMVSLGVNRENIYVVDSKGV
CCHHHHHHHHHHHHHHHCCCHHEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCE
IFQGRDANMEANKARYAQDTSARTLADIVKDADVFLGCSTAGVLTGDMVKTMADRPIILA
EEECCCCCCCCCHHHHCCCCHHHHHHHHHHCCHHEEECCCCCCHHHHHHHHHCCCCEEEE
LANPEPEIRPEVAKAARPDCIIATGRSDYPNQVNNVLCFPYIFRGALDCGATKITEAMKL
ECCCCCCCCHHHHHCCCCCEEEECCCCCCCHHHCCEEEHHHHHHHHHHCCHHHHHHHHHH
ACVKAIAELAEAELNDAVAAAYGGRELKFGPDYIIPTPFDQRLIEKIAPAVAKAAEESGV
HHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
ATRPIKDLEAYRQQLTSYVYHTGMIMKPVFTAAKAAPKRVAYAEGEEERVLRAVQGVVDE
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHH
GLARPILIGRPHVIQMRIDKAGLRLRPGVDFELINPEDDPRYRAYHEEYHALRGRDGVTP
HCCCCEEECCCEEEEEEECCCCCEECCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCH
DMAKVALRRSNTLIGTMLMHMGDADALLCGTVGRFEAHLEHVRDVIGLAPGAKVFAAMNA
HHHHHHHHHCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LMLEKYTLFITDTFVNDDPSAEELAAITQLAAEEIARFGLHPKVAMMSHSMFGSSNRPSA
HHHHHEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHH
RKMRDAAEILARVAPQLEVEGEMQGDAALVEDVRRHFLPGTKLAGSANLLVMPTLDAANI
HHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHH
AFNLLKITGGQGVTVGPILLGAAKPVHILNPQATTRRIVNMTAVAVAECNATR
EEEEEEEECCCCCEEHHEEECCCCCEEEECCCHHHHHHHHHHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 36376 [H]