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The map label for this gene is greB [H]

Identifier: 94309804

GI number: 94309804

Start: 940296

End: 940856

Strand: Reverse

Name: greB [H]

Synonym: Rmet_0859

Alternate gene names: 94309804

Gene position: 940856-940296 (Counterclockwise)

Preceding gene: 94309807

Following gene: 335055507

Centisome position: 23.95

GC content: 59.18

Gene sequence:

>561_bases
ATGAACAAGGCTTTTGTCAAAGAGTCGTCGAACGATGACGACGAAGATCTTCCCGAAGGCGCCACGCCACTGCCCGCCGG
CACCAAGAACTACATCACCGCCGCGGGCTACAAGCGCTTGCGCGACGAATTGATGCACCTGATCGACGTAGAGCGTCCGG
ACATCGTGCAGATCGTGTCGTGGGCTGCCTCGAACGGCGACCGCTCCGAGAATGGCGACTATCTTTACGGCAAGAAGCGC
ATGCGCGAAATCGACCGCCGCATTCGCTTCCTCACCCGTCGCCTGGACAAGGCCGAAGTCGTCGATCCTTCGATGCAGGG
CGACAACGATCAGATCTTCTTCGGGGCCACGGTCACCTATGCCAATCAGTCAGGCGAGGAAACGACGATCACGATCGTAG
GCATGGACGAGGTCGATCTCGACAGCAATCACGTAAGCTGGATTTCGCCGATCGCCAAGGCACTGATCAAGGCGCGCGAG
GGCGACACGGTGCCGCTTCGCACACCGGCCGGCATCGAACAGATCGATATTCTGGAAGTCCGCTACCCGCAGAAGTCTTA
G

Upstream 100 bases:

>100_bases
AGTTTTCAGTTTTGTGAACCCCCTTCGTGCATTTTTTTCATGTGAAGGGTTCGGCAGTCGCAACTTCCTATAATGAGAGC
CCGATGAAGTGATCCGGACC

Downstream 100 bases:

>100_bases
CCGTCGTTGCGGTCGCGGAAGATTCGGATCACATCAGGGTTGCGGCGCAGCGCCCGCATGACGTTGGCCAGGTGCAGGCG
GTTCTGTACCTGGATCACGA

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MNKAFVKESSNDDDEDLPEGATPLPAGTKNYITAAGYKRLRDELMHLIDVERPDIVQIVSWAASNGDRSENGDYLYGKKR
MREIDRRIRFLTRRLDKAEVVDPSMQGDNDQIFFGATVTYANQSGEETTITIVGMDEVDLDSNHVSWISPIAKALIKARE
GDTVPLRTPAGIEQIDILEVRYPQKS

Sequences:

>Translated_186_residues
MNKAFVKESSNDDDEDLPEGATPLPAGTKNYITAAGYKRLRDELMHLIDVERPDIVQIVSWAASNGDRSENGDYLYGKKR
MREIDRRIRFLTRRLDKAEVVDPSMQGDNDQIFFGATVTYANQSGEETTITIVGMDEVDLDSNHVSWISPIAKALIKARE
GDTVPLRTPAGIEQIDILEVRYPQKS
>Mature_186_residues
MNKAFVKESSNDDDEDLPEGATPLPAGTKNYITAAGYKRLRDELMHLIDVERPDIVQIVSWAASNGDRSENGDYLYGKKR
MREIDRRIRFLTRRLDKAEVVDPSMQGDNDQIFFGATVTYANQSGEETTITIVGMDEVDLDSNHVSWISPIAKALIKARE
GDTVPLRTPAGIEQIDILEVRYPQKS

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=140, Percent_Identity=47.1428571428571, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI87082225, Length=152, Percent_Identity=37.5, Blast_Score=101, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 20902; Mature: 20902

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKAFVKESSNDDDEDLPEGATPLPAGTKNYITAAGYKRLRDELMHLIDVERPDIVQIVS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCHHHHHH
WAASNGDRSENGDYLYGKKRMREIDRRIRFLTRRLDKAEVVDPSMQGDNDQIFFGATVTY
HHHCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCEEEEEEEEEE
ANQSGEETTITIVGMDEVDLDSNHVSWISPIAKALIKAREGDTVPLRTPAGIEQIDILEV
CCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEE
RYPQKS
CCCCCC
>Mature Secondary Structure
MNKAFVKESSNDDDEDLPEGATPLPAGTKNYITAAGYKRLRDELMHLIDVERPDIVQIVS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCHHHHHH
WAASNGDRSENGDYLYGKKRMREIDRRIRFLTRRLDKAEVVDPSMQGDNDQIFFGATVTY
HHHCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCEEEEEEEEEE
ANQSGEETTITIVGMDEVDLDSNHVSWISPIAKALIKAREGDTVPLRTPAGIEQIDILEV
CCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEE
RYPQKS
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]