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The map label for this gene is spoT [H]

Identifier: 94309803

GI number: 94309803

Start: 937675

End: 940299

Strand: Direct

Name: spoT [H]

Synonym: Rmet_0858

Alternate gene names: 94309803

Gene position: 937675-940299 (Clockwise)

Preceding gene: 94309802

Following gene: 94309805

Centisome position: 23.87

GC content: 64.19

Gene sequence:

>2625_bases
ATGACTTTCCCGCCGCCCGATCCTGCCGTTGCTCCGCCCGCCGAACCGGCGTCGCGGGCTCCGCATGGCCGGCCGGCGAC
CAGCGGGCAGTCCGCCTCCGCCACGACAGTGGGTTCCGGAGCGCCGCTGCCGGCTTCCTCCGCACTGGCGTCCACACCAT
CGCAAGCCGGACCGACCGGACGTAAGCGTCCAGGACAATCCACTGTGACTGCCCGTACCGGTGCTCCCAATCTTCCCGAT
CCGCTCGGCGACAACGTCGTCGGCGAGCGTGCCGTCGTACCGCCGATCCAGCAGGGCAAGTCACGCACCGCGTTGACCAA
CGAGCTCAATGCCGAGCCCGTGGCTGGCACGGTGCCCGCGGCTCCCGGTGGCGATCTGTTCATCGACGCCGTGCTGGCGC
AGTCTTATCGGCACTTTTTCGGGCCGACCTCGCAGCCGGCGGTGCCGCCGCGCCAACAGGTCATCTCGATCACGCGGCTG
ATGGAGAAGCTCGCTTACCTGAAGCCTGCCGATCAGGCCCTGGTGCGCGAGGCATTCCAGTTTTCCGACGAGGCCCACCT
GGGCCAGTACCGGCAAAGTGGGGAACCGTACATCACGCACCCGGTGGCGGTGGCCGAGCTTTGTGCCGACTGGAAGCTCG
ATGTGCAATCCATCATGGCGGCGCTGCTGCACGACGTGATGGAGGACCAGGGCATCACGAAGAGCGAACTGGCCGAGAAA
TTCGGTCCGAAGGTCTCCGAACTCGTAGATGGTCTGACCAAGCTGGACAAGCTCGAATTCCAGAGCCGGGAGCAGGCACA
GGCGGAGAGCTTCCGCAAGATGCTGCTGGCGATGGCGCGTGACGTGCGGGTGATTCTGGTCAAGCTGGCCGATCGCACGC
ACAACATGCGCACGCTCGATTTCGTGCCGCCCGAGAAGCGGCGACGGATCGCGCTGGAGACCATGGAGATCTACGCGCCG
ATCGCGCATCGGTTGGGTCTCAATACGATTTATCGCGAGCTGCAGGAACTGTCGTTCAAGGTCGGCTCGCCATTCCGCTA
CGCCACGCTCGAGAAAGCCGTGAAGGCCGCGCGCGGCAACCGGCGCGAAGTGGTCAAGCGGATTCTGGAAGCCGCCCAGT
CCGCGCTGGCCGGGGCCGGCATCGTGGCGGAATTGTCGGGCCGCGAGAAGACGCTCTACAGCATCTACCGCAAGATGCAC
GACAAGCAGTTGTCGTTCTCGCAGGTACTCGATGTCTATGGTTTCCGCGTGGTCGTGGAAACGCAGATGCACTGCTACAT
GGCGATGGGCGCGCTGCACGGCCTTTACAAGCCGATGCCGGGCAAGTTCAAGGATTACATCGCCATTCCGAAGATCAACG
GCTACCAGTCGCTGCACACGACTTTGGTTGGCCCGTTCGGCACGCCGGTGGAGTTCCAGATCCGCACCCGTGGGATGCAC
CAGATCGCCGAGGCCGGCGTGGCTGCGCACTGGATGTACAAGCACCAGGCAGACCATGCCAACGATATCCAGCAGCAGGC
GCACCAGTGGCTGCAGTCGCTGCTCGATATCCAGAGCCAGACCGGCGATTCGCAGGAATTCCTCGAACACGTCAAGATCG
ATCTGTTCCCGGACGCCGTGTACGTGTTCACGCCGAAGGGGCATATTCGGGCGCTGCCCCGCGGCGCGACGGCACTCGAT
TTTGCCTACGCGGTCCACAGCGACCTGGGCAACCAGTGTGTGGCGGTCAAGATCAACAACGAGCTTCTGCCGTTGCGCAC
GGAGCTCAAGAGTGGCGACATCGTCGAGGTCGTGACGGCGCCGTACTCGAAGCCGAATCCGTCATGGCTGGCGTTCGTGC
GCACGGGCAAGGCGCGCGCGGCGATTCGCCACTACCTGAAGACCACCAAGCTCGACGAGGCGATCCAGCTCGGCGAGCGC
CTGCTGGAACAATCAACGCGCCAGCTCGGCATCGAGCTCAAGGCGGTGCCACAGTCGGTCTGGGATCGCATGATCCAGTG
GACAGGCAACAAGCTCAAGGAAGACATCTTCGCCGACCTCGCGCTGGGACGCCGTGTGCCCGCAGTGGTCGCGCGCCGGA
TGGAAATCCTGCTGCAGGAACTGTCGGGAGATGTCGATAGCGCGTTGCTGGCGGCCGTCCAGACGTTCGCGGGGGAAGAT
GCGCCGGCCGTGCCGATTACCGGCGACGAAGGCATGTCGATGATCTTCTCGGCTTGCTGCCGTCCGATCCCCGGCGATTC
GATCGTCGGCTACCTCGGCAAGGGCGAAGGGCTGCAGATCCACGTGCAGGATTGCAAGGTGGCCAAGCGGCTGCACAGCA
AGGACCCGGAGCACTGGATCGAGGTGATGTGGGCCAAGAAGACGACGCGCGCGTTCGACGTTTCGATCAAGATCATGGTC
CGCAACGTCAAGGGTATCGTGGCCCGCGTCGCTGCGGACCTGACCTCCGCGGATGCCAACGTCGCGCACGTGGCGATGGA
ACCGCAGCAGGGCGGCCATCAGGAGGCTACCTACATGCAGTTCGTGATCCAGGTACAGAACCGCCTGCACCTGGCCAACG
TCATGCGGGCGCTGCGCCGCAACCCTGATGTGATCCGAATCTTCCGCGACCGCAACGACGGCTAA

Upstream 100 bases:

>100_bases
AAGGTACCGACCTGATTGCCGGGACGGCCTGTGGTTCATCGTCGCTTCGCATATATGCGCTTTCAGCCGCGCGTTCCGCT
GAATGACAGGGGCCGTCCCC

Downstream 100 bases:

>100_bases
GACTTCTGCGGGTAGCGGACTTCCAGAATATCGATCTGTTCGATGCCGGCCGGTGTGCGAAGCGGCACCGTGTCGCCCTC
GCGCGCCTTGATCAGTGCCT

Product: bifunctional (p)ppGpp synthetase II/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 874; Mature: 873

Protein sequence:

>874_residues
MTFPPPDPAVAPPAEPASRAPHGRPATSGQSASATTVGSGAPLPASSALASTPSQAGPTGRKRPGQSTVTARTGAPNLPD
PLGDNVVGERAVVPPIQQGKSRTALTNELNAEPVAGTVPAAPGGDLFIDAVLAQSYRHFFGPTSQPAVPPRQQVISITRL
MEKLAYLKPADQALVREAFQFSDEAHLGQYRQSGEPYITHPVAVAELCADWKLDVQSIMAALLHDVMEDQGITKSELAEK
FGPKVSELVDGLTKLDKLEFQSREQAQAESFRKMLLAMARDVRVILVKLADRTHNMRTLDFVPPEKRRRIALETMEIYAP
IAHRLGLNTIYRELQELSFKVGSPFRYATLEKAVKAARGNRREVVKRILEAAQSALAGAGIVAELSGREKTLYSIYRKMH
DKQLSFSQVLDVYGFRVVVETQMHCYMAMGALHGLYKPMPGKFKDYIAIPKINGYQSLHTTLVGPFGTPVEFQIRTRGMH
QIAEAGVAAHWMYKHQADHANDIQQQAHQWLQSLLDIQSQTGDSQEFLEHVKIDLFPDAVYVFTPKGHIRALPRGATALD
FAYAVHSDLGNQCVAVKINNELLPLRTELKSGDIVEVVTAPYSKPNPSWLAFVRTGKARAAIRHYLKTTKLDEAIQLGER
LLEQSTRQLGIELKAVPQSVWDRMIQWTGNKLKEDIFADLALGRRVPAVVARRMEILLQELSGDVDSALLAAVQTFAGED
APAVPITGDEGMSMIFSACCRPIPGDSIVGYLGKGEGLQIHVQDCKVAKRLHSKDPEHWIEVMWAKKTTRAFDVSIKIMV
RNVKGIVARVAADLTSADANVAHVAMEPQQGGHQEATYMQFVIQVQNRLHLANVMRALRRNPDVIRIFRDRNDG

Sequences:

>Translated_874_residues
MTFPPPDPAVAPPAEPASRAPHGRPATSGQSASATTVGSGAPLPASSALASTPSQAGPTGRKRPGQSTVTARTGAPNLPD
PLGDNVVGERAVVPPIQQGKSRTALTNELNAEPVAGTVPAAPGGDLFIDAVLAQSYRHFFGPTSQPAVPPRQQVISITRL
MEKLAYLKPADQALVREAFQFSDEAHLGQYRQSGEPYITHPVAVAELCADWKLDVQSIMAALLHDVMEDQGITKSELAEK
FGPKVSELVDGLTKLDKLEFQSREQAQAESFRKMLLAMARDVRVILVKLADRTHNMRTLDFVPPEKRRRIALETMEIYAP
IAHRLGLNTIYRELQELSFKVGSPFRYATLEKAVKAARGNRREVVKRILEAAQSALAGAGIVAELSGREKTLYSIYRKMH
DKQLSFSQVLDVYGFRVVVETQMHCYMAMGALHGLYKPMPGKFKDYIAIPKINGYQSLHTTLVGPFGTPVEFQIRTRGMH
QIAEAGVAAHWMYKHQADHANDIQQQAHQWLQSLLDIQSQTGDSQEFLEHVKIDLFPDAVYVFTPKGHIRALPRGATALD
FAYAVHSDLGNQCVAVKINNELLPLRTELKSGDIVEVVTAPYSKPNPSWLAFVRTGKARAAIRHYLKTTKLDEAIQLGER
LLEQSTRQLGIELKAVPQSVWDRMIQWTGNKLKEDIFADLALGRRVPAVVARRMEILLQELSGDVDSALLAAVQTFAGED
APAVPITGDEGMSMIFSACCRPIPGDSIVGYLGKGEGLQIHVQDCKVAKRLHSKDPEHWIEVMWAKKTTRAFDVSIKIMV
RNVKGIVARVAADLTSADANVAHVAMEPQQGGHQEATYMQFVIQVQNRLHLANVMRALRRNPDVIRIFRDRNDG
>Mature_873_residues
TFPPPDPAVAPPAEPASRAPHGRPATSGQSASATTVGSGAPLPASSALASTPSQAGPTGRKRPGQSTVTARTGAPNLPDP
LGDNVVGERAVVPPIQQGKSRTALTNELNAEPVAGTVPAAPGGDLFIDAVLAQSYRHFFGPTSQPAVPPRQQVISITRLM
EKLAYLKPADQALVREAFQFSDEAHLGQYRQSGEPYITHPVAVAELCADWKLDVQSIMAALLHDVMEDQGITKSELAEKF
GPKVSELVDGLTKLDKLEFQSREQAQAESFRKMLLAMARDVRVILVKLADRTHNMRTLDFVPPEKRRRIALETMEIYAPI
AHRLGLNTIYRELQELSFKVGSPFRYATLEKAVKAARGNRREVVKRILEAAQSALAGAGIVAELSGREKTLYSIYRKMHD
KQLSFSQVLDVYGFRVVVETQMHCYMAMGALHGLYKPMPGKFKDYIAIPKINGYQSLHTTLVGPFGTPVEFQIRTRGMHQ
IAEAGVAAHWMYKHQADHANDIQQQAHQWLQSLLDIQSQTGDSQEFLEHVKIDLFPDAVYVFTPKGHIRALPRGATALDF
AYAVHSDLGNQCVAVKINNELLPLRTELKSGDIVEVVTAPYSKPNPSWLAFVRTGKARAAIRHYLKTTKLDEAIQLGERL
LEQSTRQLGIELKAVPQSVWDRMIQWTGNKLKEDIFADLALGRRVPAVVARRMEILLQELSGDVDSALLAAVQTFAGEDA
PAVPITGDEGMSMIFSACCRPIPGDSIVGYLGKGEGLQIHVQDCKVAKRLHSKDPEHWIEVMWAKKTTRAFDVSIKIMVR
NVKGIVARVAADLTSADANVAHVAMEPQQGGHQEATYMQFVIQVQNRLHLANVMRALRRNPDVIRIFRDRNDG

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=721, Percent_Identity=46.7406380027739, Blast_Score=663, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=684, Percent_Identity=32.0175438596491, Blast_Score=382, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 96211; Mature: 96079

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFPPPDPAVAPPAEPASRAPHGRPATSGQSASATTVGSGAPLPASSALASTPSQAGPTG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCC
RKRPGQSTVTARTGAPNLPDPLGDNVVGERAVVPPIQQGKSRTALTNELNAEPVAGTVPA
CCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCHHHHHCCCCCCCCCCCCC
APGGDLFIDAVLAQSYRHFFGPTSQPAVPPRQQVISITRLMEKLAYLKPADQALVREAFQ
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
FSDEAHLGQYRQSGEPYITHPVAVAELCADWKLDVQSIMAALLHDVMEDQGITKSELAEK
CCCHHHHHHHHHCCCCEEECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
FGPKVSELVDGLTKLDKLEFQSREQAQAESFRKMLLAMARDVRVILVKLADRTHNMRTLD
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
FVPPEKRRRIALETMEIYAPIAHRLGLNTIYRELQELSFKVGSPFRYATLEKAVKAARGN
CCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
RREVVKRILEAAQSALAGAGIVAELSGREKTLYSIYRKMHDKQLSFSQVLDVYGFRVVVE
HHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCEEEEE
TQMHCYMAMGALHGLYKPMPGKFKDYIAIPKINGYQSLHTTLVGPFGTPVEFQIRTRGMH
HHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHCCCCCCCEEEEEHHHHHH
QIAEAGVAAHWMYKHQADHANDIQQQAHQWLQSLLDIQSQTGDSQEFLEHVKIDLFPDAV
HHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEECCEE
YVFTPKGHIRALPRGATALDFAYAVHSDLGNQCVAVKINNELLPLRTELKSGDIVEVVTA
EEECCCCCEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCEECHHHHCCCCCEEEEEEC
PYSKPNPSWLAFVRTGKARAAIRHYLKTTKLDEAIQLGERLLEQSTRQLGIELKAVPQSV
CCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
WDRMIQWTGNKLKEDIFADLALGRRVPAVVARRMEILLQELSGDVDSALLAAVQTFAGED
HHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
APAVPITGDEGMSMIFSACCRPIPGDSIVGYLGKGEGLQIHVQDCKVAKRLHSKDPEHWI
CCCCCCCCCCHHHHHHHHHCCCCCCCCCEEECCCCCCEEEEHHHHHHHHHHHCCCHHHHH
EVMWAKKTTRAFDVSIKIMVRNVKGIVARVAADLTSADANVAHVAMEPQQGGHQEATYMQ
HHHHHHCCCCEEEEEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHH
FVIQVQNRLHLANVMRALRRNPDVIRIFRDRNDG
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
>Mature Secondary Structure 
TFPPPDPAVAPPAEPASRAPHGRPATSGQSASATTVGSGAPLPASSALASTPSQAGPTG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCC
RKRPGQSTVTARTGAPNLPDPLGDNVVGERAVVPPIQQGKSRTALTNELNAEPVAGTVPA
CCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCHHHHHCCCCCCCCCCCCC
APGGDLFIDAVLAQSYRHFFGPTSQPAVPPRQQVISITRLMEKLAYLKPADQALVREAFQ
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
FSDEAHLGQYRQSGEPYITHPVAVAELCADWKLDVQSIMAALLHDVMEDQGITKSELAEK
CCCHHHHHHHHHCCCCEEECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
FGPKVSELVDGLTKLDKLEFQSREQAQAESFRKMLLAMARDVRVILVKLADRTHNMRTLD
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
FVPPEKRRRIALETMEIYAPIAHRLGLNTIYRELQELSFKVGSPFRYATLEKAVKAARGN
CCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
RREVVKRILEAAQSALAGAGIVAELSGREKTLYSIYRKMHDKQLSFSQVLDVYGFRVVVE
HHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCEEEEE
TQMHCYMAMGALHGLYKPMPGKFKDYIAIPKINGYQSLHTTLVGPFGTPVEFQIRTRGMH
HHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHCCCCCCCEEEEEHHHHHH
QIAEAGVAAHWMYKHQADHANDIQQQAHQWLQSLLDIQSQTGDSQEFLEHVKIDLFPDAV
HHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEECCEE
YVFTPKGHIRALPRGATALDFAYAVHSDLGNQCVAVKINNELLPLRTELKSGDIVEVVTA
EEECCCCCEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCEECHHHHCCCCCEEEEEEC
PYSKPNPSWLAFVRTGKARAAIRHYLKTTKLDEAIQLGERLLEQSTRQLGIELKAVPQSV
CCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
WDRMIQWTGNKLKEDIFADLALGRRVPAVVARRMEILLQELSGDVDSALLAAVQTFAGED
HHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
APAVPITGDEGMSMIFSACCRPIPGDSIVGYLGKGEGLQIHVQDCKVAKRLHSKDPEHWI
CCCCCCCCCCHHHHHHHHHCCCCCCCCCEEECCCCCCEEEEHHHHHHHHHHHCCCHHHHH
EVMWAKKTTRAFDVSIKIMVRNVKGIVARVAADLTSADANVAHVAMEPQQGGHQEATYMQ
HHHHHHCCCCEEEEEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHH
FVIQVQNRLHLANVMRALRRNPDVIRIFRDRNDG
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]