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The map label for this gene is gudD [H]

Identifier: 94309779

GI number: 94309779

Start: 911378

End: 912733

Strand: Direct

Name: gudD [H]

Synonym: Rmet_0834

Alternate gene names: 94309779

Gene position: 911378-912733 (Clockwise)

Preceding gene: 94309776

Following gene: 94309780

Centisome position: 23.2

GC content: 64.97

Gene sequence:

>1356_bases
ATGAATGCCGCTTCCCTTCAAGGCGCCGCTGGTGCCACGCCTGTCGTCACTGAACTGACCGTTGTCCCCGTCGCCGGCCA
CGACAGCATGCTGATGAACCTGTCGGGGGCCCACGGCCCGTACTTCACCCGCAACATCGTGATTCTGCGCGACAGCGCAG
GCAATACCGGCGTTGGCGAAGTGCCGGGCGGCGAGGGCATTCGCAAGACGCTCGAGGACGCGCGCCCGCTCGTGGTGGGT
CAACCGATCGGCCAGTATCAGGCGATGCTGAACAAGGTTCGAGCCACGTTCGCGAGCCGCGATGCCGGGGGACGTGGTCT
GCAGACCTTCGACCTGCGCATCACGATCCATGCCGTGACCGCGCTTGAAGCTGCGCTGCTCGATCTGCTCGGCAAGCACC
TTCAGGTGCCCGTGGCGGCCCTGCTCGGCGAAGGCCAGCAGCGCGACGCGGTGGAGATGCTGGGCTACCTGTTCTACGTC
GGTGATCGCCAGCGTACGACGCTTGACTATCGCACCGAGCCCGATGCGGACAACGAATGGTTCCGCCTGCGCAATGAAGT
GGCGATGGATGCGAAAGGCGTGGTGCGACTGGCCGAGGCCGCGTACGAGCGCTACGGCTTCAACGATTTCAAGCTCAAGG
GTGGCGTGCTGAGCGGTGACGAGGAAATGGAGGCGATCCTCGCGCTGCATGAGCGTTTTCCGAAAGCACGCGTCACGCTC
GACCCGAACGGCGGCTGGCTGCTGGCCGACGCGATCCGCCTCTGCCGCGACAAGCATGGCGTGCTGGCCTATGCGGAGGA
TCCCTGCGGCGCCGAGGACGGCTATTCGGGCCGCGAGATCATGGCCGAGTTCCGCACTGCCACCGGACTACCTACGGCCA
CCAACATGATCGCCACCGACTGGCGTCAGATGGGTCATGCGGTGCGCCTGCATTCGGTCGACATTCCGCTGGCCGATCCG
CACTTCTGGACCATGCAGGGCTCGGTCCGCGTGGCGCAGATGTGCGCGGAGTGGGGGCTGACCTGGGGCTCGCACTCGAA
CAATCACTTCGATATTTCGCTGGCGATGTTCACGCATGTGGCCGCCGCCGCGCCGGGCCGCGTGACGGCAATCGACACGC
ACTGGATCTGGCAGGACGGTGAGCACCTGACACGCAACCCGCTCAGGATCGAAGGTGGCCTGGTGCAGGTACCGAAGACA
CCTGGTCTGGGCGTGGAGCTGGATATGGATGCGCTCGCGCGCGCCAACCGCCTCTATCAGGAAAAGGGACTCGGCGCGCG
CGACGATGCGATCGCGATGCAGTTCCTGATCCCTGGCTGGAAGTTCAACAACAAGATGCCCTGCATGGTGCGCTGA

Upstream 100 bases:

>100_bases
CGCTCATTATACTTCGTCATAAGTCATACGACGACCGATAACACACAGGTGCTTGCAGCTACCCTCACGTTGTGGCAGCG
CCGGCCCGGAGACAACCGAC

Downstream 100 bases:

>100_bases
CGGGCCATGCCGCGTGGCGGGGGCAGTGGGTGTTTCGCCACGACAGACAGATAACAACAAGCCAAACGCTTTTCGTCAAA
GCAGGAGACAAACGATGTTC

Product: (D)-glucarate dehydratase 1

Products: NA

Alternate protein names: GDH; GlucD [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MNAASLQGAAGATPVVTELTVVPVAGHDSMLMNLSGAHGPYFTRNIVILRDSAGNTGVGEVPGGEGIRKTLEDARPLVVG
QPIGQYQAMLNKVRATFASRDAGGRGLQTFDLRITIHAVTALEAALLDLLGKHLQVPVAALLGEGQQRDAVEMLGYLFYV
GDRQRTTLDYRTEPDADNEWFRLRNEVAMDAKGVVRLAEAAYERYGFNDFKLKGGVLSGDEEMEAILALHERFPKARVTL
DPNGGWLLADAIRLCRDKHGVLAYAEDPCGAEDGYSGREIMAEFRTATGLPTATNMIATDWRQMGHAVRLHSVDIPLADP
HFWTMQGSVRVAQMCAEWGLTWGSHSNNHFDISLAMFTHVAAAAPGRVTAIDTHWIWQDGEHLTRNPLRIEGGLVQVPKT
PGLGVELDMDALARANRLYQEKGLGARDDAIAMQFLIPGWKFNNKMPCMVR

Sequences:

>Translated_451_residues
MNAASLQGAAGATPVVTELTVVPVAGHDSMLMNLSGAHGPYFTRNIVILRDSAGNTGVGEVPGGEGIRKTLEDARPLVVG
QPIGQYQAMLNKVRATFASRDAGGRGLQTFDLRITIHAVTALEAALLDLLGKHLQVPVAALLGEGQQRDAVEMLGYLFYV
GDRQRTTLDYRTEPDADNEWFRLRNEVAMDAKGVVRLAEAAYERYGFNDFKLKGGVLSGDEEMEAILALHERFPKARVTL
DPNGGWLLADAIRLCRDKHGVLAYAEDPCGAEDGYSGREIMAEFRTATGLPTATNMIATDWRQMGHAVRLHSVDIPLADP
HFWTMQGSVRVAQMCAEWGLTWGSHSNNHFDISLAMFTHVAAAAPGRVTAIDTHWIWQDGEHLTRNPLRIEGGLVQVPKT
PGLGVELDMDALARANRLYQEKGLGARDDAIAMQFLIPGWKFNNKMPCMVR
>Mature_451_residues
MNAASLQGAAGATPVVTELTVVPVAGHDSMLMNLSGAHGPYFTRNIVILRDSAGNTGVGEVPGGEGIRKTLEDARPLVVG
QPIGQYQAMLNKVRATFASRDAGGRGLQTFDLRITIHAVTALEAALLDLLGKHLQVPVAALLGEGQQRDAVEMLGYLFYV
GDRQRTTLDYRTEPDADNEWFRLRNEVAMDAKGVVRLAEAAYERYGFNDFKLKGGVLSGDEEMEAILALHERFPKARVTL
DPNGGWLLADAIRLCRDKHGVLAYAEDPCGAEDGYSGREIMAEFRTATGLPTATNMIATDWRQMGHAVRLHSVDIPLADP
HFWTMQGSVRVAQMCAEWGLTWGSHSNNHFDISLAMFTHVAAAAPGRVTAIDTHWIWQDGEHLTRNPLRIEGGLVQVPKT
PGLGVELDMDALARANRLYQEKGLGARDDAIAMQFLIPGWKFNNKMPCMVR

Specific function: Catalyzes the dehydration of glucarate to 5-keto-4- deoxy-D-glucarate (5-kdGluc) [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789150, Length=440, Percent_Identity=73.6363636363636, Blast_Score=694, Evalue=0.0,
Organism=Escherichia coli, GI1789151, Length=446, Percent_Identity=60.762331838565, Blast_Score=541, Evalue=1e-155,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017653
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.40 [H]

Molecular weight: Translated: 49307; Mature: 49307

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAASLQGAAGATPVVTELTVVPVAGHDSMLMNLSGAHGPYFTRNIVILRDSAGNTGVGE
CCCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEECCCCCCEEEEEEEEEEECCCCCCCCC
VPGGEGIRKTLEDARPLVVGQPIGQYQAMLNKVRATFASRDAGGRGLQTFDLRITIHAVT
CCCCHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEHHH
ALEAALLDLLGKHLQVPVAALLGEGQQRDAVEMLGYLFYVGDRQRTTLDYRTEPDADNEW
HHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHEEECCCCEEEEEECCCCCCCCHH
FRLRNEVAMDAKGVVRLAEAAYERYGFNDFKLKGGVLSGDEEMEAILALHERFPKARVTL
HHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEE
DPNGGWLLADAIRLCRDKHGVLAYAEDPCGAEDGYSGREIMAEFRTATGLPTATNMIATD
CCCCCEEHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHH
WRQMGHAVRLHSVDIPLADPHFWTMQGSVRVAQMCAEWGLTWGSHSNNHFDISLAMFTHV
HHHCCCEEEEEEECCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHH
AAAAPGRVTAIDTHWIWQDGEHLTRNPLRIEGGLVQVPKTPGLGVELDMDALARANRLYQ
HHCCCCCEEEEEEEEEECCCCCCCCCCEEEECCEEECCCCCCCCEEECHHHHHHHHHHHH
EKGLGARDDAIAMQFLIPGWKFNNKMPCMVR
HCCCCCCCCCEEEEEEECCEECCCCCCCEEC
>Mature Secondary Structure
MNAASLQGAAGATPVVTELTVVPVAGHDSMLMNLSGAHGPYFTRNIVILRDSAGNTGVGE
CCCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEECCCCCCEEEEEEEEEEECCCCCCCCC
VPGGEGIRKTLEDARPLVVGQPIGQYQAMLNKVRATFASRDAGGRGLQTFDLRITIHAVT
CCCCHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEHHH
ALEAALLDLLGKHLQVPVAALLGEGQQRDAVEMLGYLFYVGDRQRTTLDYRTEPDADNEW
HHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHEEECCCCEEEEEECCCCCCCCHH
FRLRNEVAMDAKGVVRLAEAAYERYGFNDFKLKGGVLSGDEEMEAILALHERFPKARVTL
HHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEE
DPNGGWLLADAIRLCRDKHGVLAYAEDPCGAEDGYSGREIMAEFRTATGLPTATNMIATD
CCCCCEEHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHH
WRQMGHAVRLHSVDIPLADPHFWTMQGSVRVAQMCAEWGLTWGSHSNNHFDISLAMFTHV
HHHCCCEEEEEEECCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHH
AAAAPGRVTAIDTHWIWQDGEHLTRNPLRIEGGLVQVPKTPGLGVELDMDALARANRLYQ
HHCCCCCEEEEEEEEEECCCCCCCCCCEEEECCEEECCCCCCCCEEECHHHHHHHHHHHH
EKGLGARDDAIAMQFLIPGWKFNNKMPCMVR
HCCCCCCCCCEEEEEEECCEECCCCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9772161 [H]