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The map label for this gene is rng [H]

Identifier: 94309723

GI number: 94309723

Start: 854689

End: 856221

Strand: Reverse

Name: rng [H]

Synonym: Rmet_0778

Alternate gene names: 94309723

Gene position: 856221-854689 (Counterclockwise)

Preceding gene: 94309724

Following gene: 94309720

Centisome position: 21.8

GC content: 62.75

Gene sequence:

>1533_bases
ATGCCCGGCCACGAGCCGGGCCACAACAACAAGCGCCAACGGATCGGCGACGCGAGATTAAGCGTATCCATCGCTATGAC
CGAAGACATCCTCGTCAACATCACGCCGCAGGAAACACGCGTCGCCATCGTGCAGCAGGCGGCCGTTCAGGAACTCCATG
TCGAACGCACGCTAACGCGCGGGCTGGTCGGCAACATCTATCTGGGCAAGGTCGTGCGCGTGCTGCCGGGGATGCAATCG
GCATTCATCGACGTCGGCCTGGAGCGCGCCGCGTTCCTGCACGTGGCCGACATCTGGCATCCGCGTGACGGCGACAAGAA
CGGCCACCTCGCCATCGAGAAAACGCTGTTCGAGGGCCAGGCACTGATGGTTCAGGTCATCAAGGACCCGATCGGCACCA
AGGGCGCGCGGTTGTCCACACAGGTCAGCATCGCAGGCCGCACACTCGTCTATCTGCCGCAGGATCCGCATATCGGCATC
TCGCAGCGTATCGAGGGTGAAATCGACCGCGAGGCGCTCCGTGCCCGCGTGCAGGGCCTGGTGCCCACCGACGAGCGCGG
CGGCTTCATCGTGCGCACGATCGCCGAGGAAGCCAGCGACGAGGAACTGGGCAACGACATCGCCTACCTGCGCAAGATCT
GGGCGACGATCCGCTCCAACGCCACCACGCTGCCCGCGCCGAGCCTGCTCTATCAGGACCTGAACCTGGCCCAGCGCGTG
CTGCGCGACTTCATCAACGATGCCACTGGCGTGATCCAGATCGATTCGCGCGAGAACTACCAGAAACTGGTGGAGTTCGC
GCAGGAATACACGCCCGCCGTGCTGCCGCGTCTGTCGCACTACACCGGCGAGCGCCCAATCTTCGACCTGTTCAACATCG
ACGCGGAAATCGAAAAGGCGCTGTCGCGCCGGGTAGACCTGAAGTCCGGCGGCTACCTGATGATCGATCAGACCGAGGCG
ATGACAACGATCGACGTCAACACGGGCGGCTATGTCGGTGCGCGCAACTTCGACGATACGATCTTCAAGACGAATCTCGA
AGCCGCGCACACCATCGCGCGCCAGCTCCGGCTGCGCAACCTGGGCGGCATCATCATCATCGACTTCATTGACATGGAGA
ACGTGGAGCATCGCGACGCGGTGCTCTCCGAACTGAAGCGCGCGCTGTCACGCGACCGCACGCGCATCACGGTCAACGGC
TTCTCGCAACTCGGTCTGGTGGAGATGACGCGCAAGCGCACGCGCGAATCGCTCGCGCATGTGCTGTGCGAGCAGTGCCC
GGTCTGCACCGGCAAGGGCCAGGTCAAGACGCCGCGAACGGTCTGCTACGACATCTTCCGCGAAATCATGCGCGAGTCGC
GCCAGTTCAATCCGCGCGAGTTCCGGATCCTGGCCTCGCAGGAAGTCATCGACCTGTTCCTCGAGGAAGAAAGCCAGCAT
CTGGCGATGCTCGGGGACTTCATCGGCAAGCCGATCTCTCTCCAGGTGGAATCGACGTTCCACCAGGAGCAATACGACAT
CATTCTGATGTAA

Upstream 100 bases:

>100_bases
GAGACGGCGGCACTGCTGCGCGAGGCCGGTTTGCACTTCTGACCGGCGCTTCCAGCCCGGCCTTCCGACCCAACCTCTCC
GACAAGGCCACTGGGGTCAA

Downstream 100 bases:

>100_bases
CCCCCACCCGCCGGCCACGCAGGGCCACGTGGAACGCCACGAGCCCTGCGATCTGCAAGCCGGCCATGCCGAGCGCCATC
TTCATCGTGGAGTCCAGCAG

Product: ribonuclease G

Products: NA

Alternate protein names: RNase G; Cytoplasmic axial filament protein [H]

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MPGHEPGHNNKRQRIGDARLSVSIAMTEDILVNITPQETRVAIVQQAAVQELHVERTLTRGLVGNIYLGKVVRVLPGMQS
AFIDVGLERAAFLHVADIWHPRDGDKNGHLAIEKTLFEGQALMVQVIKDPIGTKGARLSTQVSIAGRTLVYLPQDPHIGI
SQRIEGEIDREALRARVQGLVPTDERGGFIVRTIAEEASDEELGNDIAYLRKIWATIRSNATTLPAPSLLYQDLNLAQRV
LRDFINDATGVIQIDSRENYQKLVEFAQEYTPAVLPRLSHYTGERPIFDLFNIDAEIEKALSRRVDLKSGGYLMIDQTEA
MTTIDVNTGGYVGARNFDDTIFKTNLEAAHTIARQLRLRNLGGIIIIDFIDMENVEHRDAVLSELKRALSRDRTRITVNG
FSQLGLVEMTRKRTRESLAHVLCEQCPVCTGKGQVKTPRTVCYDIFREIMRESRQFNPREFRILASQEVIDLFLEEESQH
LAMLGDFIGKPISLQVESTFHQEQYDIILM

Sequences:

>Translated_510_residues
MPGHEPGHNNKRQRIGDARLSVSIAMTEDILVNITPQETRVAIVQQAAVQELHVERTLTRGLVGNIYLGKVVRVLPGMQS
AFIDVGLERAAFLHVADIWHPRDGDKNGHLAIEKTLFEGQALMVQVIKDPIGTKGARLSTQVSIAGRTLVYLPQDPHIGI
SQRIEGEIDREALRARVQGLVPTDERGGFIVRTIAEEASDEELGNDIAYLRKIWATIRSNATTLPAPSLLYQDLNLAQRV
LRDFINDATGVIQIDSRENYQKLVEFAQEYTPAVLPRLSHYTGERPIFDLFNIDAEIEKALSRRVDLKSGGYLMIDQTEA
MTTIDVNTGGYVGARNFDDTIFKTNLEAAHTIARQLRLRNLGGIIIIDFIDMENVEHRDAVLSELKRALSRDRTRITVNG
FSQLGLVEMTRKRTRESLAHVLCEQCPVCTGKGQVKTPRTVCYDIFREIMRESRQFNPREFRILASQEVIDLFLEEESQH
LAMLGDFIGKPISLQVESTFHQEQYDIILM
>Mature_509_residues
PGHEPGHNNKRQRIGDARLSVSIAMTEDILVNITPQETRVAIVQQAAVQELHVERTLTRGLVGNIYLGKVVRVLPGMQSA
FIDVGLERAAFLHVADIWHPRDGDKNGHLAIEKTLFEGQALMVQVIKDPIGTKGARLSTQVSIAGRTLVYLPQDPHIGIS
QRIEGEIDREALRARVQGLVPTDERGGFIVRTIAEEASDEELGNDIAYLRKIWATIRSNATTLPAPSLLYQDLNLAQRVL
RDFINDATGVIQIDSRENYQKLVEFAQEYTPAVLPRLSHYTGERPIFDLFNIDAEIEKALSRRVDLKSGGYLMIDQTEAM
TTIDVNTGGYVGARNFDDTIFKTNLEAAHTIARQLRLRNLGGIIIIDFIDMENVEHRDAVLSELKRALSRDRTRITVNGF
SQLGLVEMTRKRTRESLAHVLCEQCPVCTGKGQVKTPRTVCYDIFREIMRESRQFNPREFRILASQEVIDLFLEEESQHL
AMLGDFIGKPISLQVESTFHQEQYDIILM

Specific function: Involved in the processing of the 5'-end of 16S rRNA. Could be involved in chromosome segregation and cell division. It may be one of the components of the cytoplasmic axial filaments bundles or merely regulate the formation of this structure [H]

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm, cytoskeleton [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI87082235, Length=491, Percent_Identity=53.3604887983707, Blast_Score=516, Evalue=1e-147,
Organism=Escherichia coli, GI1787325, Length=458, Percent_Identity=38.4279475982533, Blast_Score=291, Evalue=6e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: 3.1.4.- [C]

Molecular weight: Translated: 57487; Mature: 57355

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGHEPGHNNKRQRIGDARLSVSIAMTEDILVNITPQETRVAIVQQAAVQELHVERTLTR
CCCCCCCCCCHHHHCCCCCEEEEEEEECCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
GLVGNIYLGKVVRVLPGMQSAFIDVGLERAAFLHVADIWHPRDGDKNGHLAIEKTLFEGQ
HHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHCCH
ALMVQVIKDPIGTKGARLSTQVSIAGRTLVYLPQDPHIGISQRIEGEIDREALRARVQGL
HHHHHHHHCCCCCCCCCEEEEEEECCEEEEEECCCCCCCHHHHCCCCCCHHHHHHHHHCC
VPTDERGGFIVRTIAEEASDEELGNDIAYLRKIWATIRSNATTLPAPSLLYQDLNLAQRV
CCCCCCCCEEEEEHHHCCCHHHHCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
LRDFINDATGVIQIDSRENYQKLVEFAQEYTPAVLPRLSHYTGERPIFDLFNIDAEIEKA
HHHHHCCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHCCCHHHHHH
LSRRVDLKSGGYLMIDQTEAMTTIDVNTGGYVGARNFDDTIFKTNLEAAHTIARQLRLRN
HHHCCCCCCCCEEEEECCCCEEEEEECCCCEECCCCCCCHHHHCCHHHHHHHHHHHHHHC
LGGIIIIDFIDMENVEHRDAVLSELKRALSRDRTRITVNGFSQLGLVEMTRKRTRESLAH
CCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHH
VLCEQCPVCTGKGQVKTPRTVCYDIFREIMRESRQFNPREFRILASQEVIDLFLEEESQH
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHEEEEEHHHHHHHHHHCCHHH
LAMLGDFIGKPISLQVESTFHQEQYDIILM
HHHHHHHHCCCEEEEECCHHCCCCCCEEEC
>Mature Secondary Structure 
PGHEPGHNNKRQRIGDARLSVSIAMTEDILVNITPQETRVAIVQQAAVQELHVERTLTR
CCCCCCCCCHHHHCCCCCEEEEEEEECCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
GLVGNIYLGKVVRVLPGMQSAFIDVGLERAAFLHVADIWHPRDGDKNGHLAIEKTLFEGQ
HHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHCCH
ALMVQVIKDPIGTKGARLSTQVSIAGRTLVYLPQDPHIGISQRIEGEIDREALRARVQGL
HHHHHHHHCCCCCCCCCEEEEEEECCEEEEEECCCCCCCHHHHCCCCCCHHHHHHHHHCC
VPTDERGGFIVRTIAEEASDEELGNDIAYLRKIWATIRSNATTLPAPSLLYQDLNLAQRV
CCCCCCCCEEEEEHHHCCCHHHHCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
LRDFINDATGVIQIDSRENYQKLVEFAQEYTPAVLPRLSHYTGERPIFDLFNIDAEIEKA
HHHHHCCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHCCCHHHHHH
LSRRVDLKSGGYLMIDQTEAMTTIDVNTGGYVGARNFDDTIFKTNLEAAHTIARQLRLRN
HHHCCCCCCCCEEEEECCCCEEEEEECCCCEECCCCCCCHHHHCCHHHHHHHHHHHHHHC
LGGIIIIDFIDMENVEHRDAVLSELKRALSRDRTRITVNGFSQLGLVEMTRKRTRESLAH
CCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHH
VLCEQCPVCTGKGQVKTPRTVCYDIFREIMRESRQFNPREFRILASQEVIDLFLEEESQH
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHEEEEEHHHHHHHHHHCCHHH
LAMLGDFIGKPISLQVESTFHQEQYDIILM
HHHHHHHHCCCEEEEECCHHCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]