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The map label for this gene is trmD

Identifier: 94309695

GI number: 94309695

Start: 826520

End: 827302

Strand: Direct

Name: trmD

Synonym: Rmet_0750

Alternate gene names: 94309695

Gene position: 826520-827302 (Clockwise)

Preceding gene: 94309694

Following gene: 94309696

Centisome position: 21.04

GC content: 63.86

Gene sequence:

>783_bases
ATGCAGTTCGATGTAATCACGCTGTTTCCCGAGATGTTTCGCGCGCTGACCGATTGGGGTATCACCAGCCGGGCAGCCAA
GCAGCAGCGCTACGCGTTGCGTACTTGGAATCCGCGGGATTTCACCGTGGACAACTACCGGACGATTGACGATCGGCCCT
ATGGTGGCGGCCCCGGCATGGTGATGCTGGCCAAGCCGCTCGAGGATGCGATCGACGCCGCCGCGGCGGCGCAGTCGGAT
GCTGGTGTCGGCAGGCCGCATGTGGTGCTGATGTCGCCGCAAGGCGCGACGCTGACGCACGAGAAGGTCATGGCGTTGTC
GCAACGGCCCGGCCTGGTGCTGTTGTGCGGCCGGTATGAAGCGATCGACCAGCGTCTGATCGATCGTCGCGTGGACGAGG
AAATCAGCCTCGGCGATTTTGTACTGTCGGGCGGCGAACTGCCGGCGATGGCGCTGATCGACGCGGTGGTGCGGCATCTG
CCCGGCGTGCTGGGCGATGCGCAGTCGGCGGTCCAGGACAGTTTCGTCAATGGCCTGCTTGATTGTCCGCATTACACGCG
GCCGGAAGAATATGAAGGTGTGCGGGTGCCCGATGTATTGCTCGGGGGCCATCATGCCGAGATCGAGAAGTGGCGGCGCC
GGCAGGCGTTGGCCAATACCGCGCGCAAGCGTCCCGACCTGATCGTGGCCGCGCGCGAGCAAGGATTGTTGTCCAAGGCC
GACGAGAAATTCTTGTCGGAACTGGCGGCGAAAGCAGGGCAGGAGTTCGCTCCCGCCAAGTGA

Upstream 100 bases:

>100_bases
ATCCCGTTTGTCGACGCGTTCCTGCGGACGGTGGATACACCGGCCAGGCGGATCGTGGTGGACTGGGGGCTCGACTACTG
AAGTGTCAGGGGAGGCGCGG

Downstream 100 bases:

>100_bases
AAACCCCATCCTCTGCCGGGGCCCAGCAAGTGTCTGGGGCATCCAACGCTGGCACGATGGTTATGGAGAAAACGATGAAC
ATCATCGAGCAAATCGAGAA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MQFDVITLFPEMFRALTDWGITSRAAKQQRYALRTWNPRDFTVDNYRTIDDRPYGGGPGMVMLAKPLEDAIDAAAAAQSD
AGVGRPHVVLMSPQGATLTHEKVMALSQRPGLVLLCGRYEAIDQRLIDRRVDEEISLGDFVLSGGELPAMALIDAVVRHL
PGVLGDAQSAVQDSFVNGLLDCPHYTRPEEYEGVRVPDVLLGGHHAEIEKWRRRQALANTARKRPDLIVAAREQGLLSKA
DEKFLSELAAKAGQEFAPAK

Sequences:

>Translated_260_residues
MQFDVITLFPEMFRALTDWGITSRAAKQQRYALRTWNPRDFTVDNYRTIDDRPYGGGPGMVMLAKPLEDAIDAAAAAQSD
AGVGRPHVVLMSPQGATLTHEKVMALSQRPGLVLLCGRYEAIDQRLIDRRVDEEISLGDFVLSGGELPAMALIDAVVRHL
PGVLGDAQSAVQDSFVNGLLDCPHYTRPEEYEGVRVPDVLLGGHHAEIEKWRRRQALANTARKRPDLIVAAREQGLLSKA
DEKFLSELAAKAGQEFAPAK
>Mature_260_residues
MQFDVITLFPEMFRALTDWGITSRAAKQQRYALRTWNPRDFTVDNYRTIDDRPYGGGPGMVMLAKPLEDAIDAAAAAQSD
AGVGRPHVVLMSPQGATLTHEKVMALSQRPGLVLLCGRYEAIDQRLIDRRVDEEISLGDFVLSGGELPAMALIDAVVRHL
PGVLGDAQSAVQDSFVNGLLDCPHYTRPEEYEGVRVPDVLLGGHHAEIEKWRRRQALANTARKRPDLIVAAREQGLLSKA
DEKFLSELAAKAGQEFAPAK

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=255, Percent_Identity=52.5490196078431, Blast_Score=265, Evalue=2e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_RALME (Q1LQE0)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_582905.1
- ProteinModelPortal:   Q1LQE0
- SMR:   Q1LQE0
- STRING:   Q1LQE0
- GeneID:   4037541
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_0750
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- PhylomeDB:   Q1LQE0
- ProtClustDB:   PRK00026
- BioCyc:   RMET266264:RMET_0750-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 28627; Mature: 28627

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: BINDING 117-117

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFDVITLFPEMFRALTDWGITSRAAKQQRYALRTWNPRDFTVDNYRTIDDRPYGGGPGM
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCE
VMLAKPLEDAIDAAAAAQSDAGVGRPHVVLMSPQGATLTHEKVMALSQRPGLVLLCGRYE
EEEECHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCHH
AIDQRLIDRRVDEEISLGDFVLSGGELPAMALIDAVVRHLPGVLGDAQSAVQDSFVNGLL
HHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCHHCHHHHHHHHHHHHHHH
DCPHYTRPEEYEGVRVPDVLLGGHHAEIEKWRRRQALANTARKRPDLIVAAREQGLLSKA
CCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHH
DEKFLSELAAKAGQEFAPAK
HHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MQFDVITLFPEMFRALTDWGITSRAAKQQRYALRTWNPRDFTVDNYRTIDDRPYGGGPGM
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCE
VMLAKPLEDAIDAAAAAQSDAGVGRPHVVLMSPQGATLTHEKVMALSQRPGLVLLCGRYE
EEEECHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCHH
AIDQRLIDRRVDEEISLGDFVLSGGELPAMALIDAVVRHLPGVLGDAQSAVQDSFVNGLL
HHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCHHCHHHHHHHHHHHHHHH
DCPHYTRPEEYEGVRVPDVLLGGHHAEIEKWRRRQALANTARKRPDLIVAAREQGLLSKA
CCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHH
DEKFLSELAAKAGQEFAPAK
HHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA