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The map label for this gene is dadA2 [H]

Identifier: 94309645

GI number: 94309645

Start: 772476

End: 773753

Strand: Direct

Name: dadA2 [H]

Synonym: Rmet_0700

Alternate gene names: 94309645

Gene position: 772476-773753 (Clockwise)

Preceding gene: 94309640

Following gene: 94309646

Centisome position: 19.67

GC content: 67.14

Gene sequence:

>1278_bases
ATGCGCATAGCGATCGTGGGTGCCGGTGTGGTCGGCATGACCTCCGCGTGGCGGTTGGCCAACGATGGTCACGAAGTCAC
CGTGGTCGAGCGCCATGATGGTCCGGGCGAGGAAACCAGCTTCGCCAACGGCGGCCAGCTCAGCTACAGCTATGTGGCGC
CGCTGGCCGGACCGGGCGTGTTGTCGAAGGTGCCGGGCTGGCTGCTGGATCGCGATTCACCGATGCGCTTCCGGCCATCC
ATGGACCCGTCGCAATGGCGCTGGCTGCTGGCTTTCGCTCGGGCGTGCAATGCATCGGCAAGCGAGGCCGCCACGCGCAA
GCTGCTGCGCCTGTCGTTCTACTCGCGCGATCTGATGCAGGCTTTCGTCGATCGTCCGGACGCGCGGCAGGAGGGCGGTG
GTTTCGATTTCGCGCGTAGCGGCAAGCTGATCGTGCACCGTGATGCGGCGGCCTACGAATCGGCCTGCCGCCTGCTCGAC
TATCAGGCCAGCCTTGGTTGCGAACAGCAGGCATTGGATCGCGATGCCACGGTTGCGCTGGAGCCGGCGTTGGCCGGCAT
TCGGCAGGGCATCGTCGGTGCGATCCACACGCCGAGCGAGGAAGTGGGCGATTGCCATCGCTTCTGCGTGTCACTGGCCC
ATCTGTTGCAAGGCAGGCCGGGTGTCACGCTGCGCTTCGGCACGCGTGTGACCGGACTGGTGCGCGCGGGGGATCGCGTG
ACGGGCCTGCGCACCGATGCGGGTGATATCCGCGCCGATGCGGTGATTGTGTCGGGCGGCATCGGCAGCGTGCCGTTGCT
CAAGCCGCTCGGTGTGCGGCCGATGCTGTGGCCGCTGAAGGGGTACAGCATCACGGTGCCGATCAGGCAGGGCGTGCTGG
CGCCACGTATCAGCGTGACGGACTTCGCCAACAAGATCGTCTATGCGCGCATCGGCGATACGCTGCGCGTGGCGGGGATG
GCGGATATCGTGCGCGGCGGCGTGGTGATCGACCGCGAGCGCGCGCAGACGCTGGTCACGCAGACGCGTGCGGTGTTTGG
CGACGTGAGCCATGGCGCGGACCTTGATGCATTGCAGCCGTGGGCCGGCCTGCGTCCGGCCACGCCCACGGGTTTGCCGA
TGATCGGCGAATCACGCATTCGCGGTCTGTGGCTGAACCTTGGCCACGGCGCGCTGGGTTTCACGCTGGCCATGGGCAGC
GCGGGTGTGCTGGCCGATCGGATGGCTGGCCGCAAGCCGGTGGTGGATGCCGAAGATTTCAGTGCAGCGCTCGCATAG

Upstream 100 bases:

>100_bases
ATAGCTTGAGGACATATTCTGGCACGTATTGGCATTGGGCAAGCCGCGCCGGGTGCTCTACGCTCTGGCCCATCGTTTGA
ACAGGACGCAAGGCAAGCGG

Downstream 100 bases:

>100_bases
TCATTCTCGCGGGCGCACACGGGTTGTGCTGGAAGTCGCAGTTTGAGAGTCGTGCCATTAAATCAAGCCCCAACCAAGGA
GCCGAACCATGAAGTCACTG

Product: D-amino acid dehydrogenase small subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MRIAIVGAGVVGMTSAWRLANDGHEVTVVERHDGPGEETSFANGGQLSYSYVAPLAGPGVLSKVPGWLLDRDSPMRFRPS
MDPSQWRWLLAFARACNASASEAATRKLLRLSFYSRDLMQAFVDRPDARQEGGGFDFARSGKLIVHRDAAAYESACRLLD
YQASLGCEQQALDRDATVALEPALAGIRQGIVGAIHTPSEEVGDCHRFCVSLAHLLQGRPGVTLRFGTRVTGLVRAGDRV
TGLRTDAGDIRADAVIVSGGIGSVPLLKPLGVRPMLWPLKGYSITVPIRQGVLAPRISVTDFANKIVYARIGDTLRVAGM
ADIVRGGVVIDRERAQTLVTQTRAVFGDVSHGADLDALQPWAGLRPATPTGLPMIGESRIRGLWLNLGHGALGFTLAMGS
AGVLADRMAGRKPVVDAEDFSAALA

Sequences:

>Translated_425_residues
MRIAIVGAGVVGMTSAWRLANDGHEVTVVERHDGPGEETSFANGGQLSYSYVAPLAGPGVLSKVPGWLLDRDSPMRFRPS
MDPSQWRWLLAFARACNASASEAATRKLLRLSFYSRDLMQAFVDRPDARQEGGGFDFARSGKLIVHRDAAAYESACRLLD
YQASLGCEQQALDRDATVALEPALAGIRQGIVGAIHTPSEEVGDCHRFCVSLAHLLQGRPGVTLRFGTRVTGLVRAGDRV
TGLRTDAGDIRADAVIVSGGIGSVPLLKPLGVRPMLWPLKGYSITVPIRQGVLAPRISVTDFANKIVYARIGDTLRVAGM
ADIVRGGVVIDRERAQTLVTQTRAVFGDVSHGADLDALQPWAGLRPATPTGLPMIGESRIRGLWLNLGHGALGFTLAMGS
AGVLADRMAGRKPVVDAEDFSAALA
>Mature_425_residues
MRIAIVGAGVVGMTSAWRLANDGHEVTVVERHDGPGEETSFANGGQLSYSYVAPLAGPGVLSKVPGWLLDRDSPMRFRPS
MDPSQWRWLLAFARACNASASEAATRKLLRLSFYSRDLMQAFVDRPDARQEGGGFDFARSGKLIVHRDAAAYESACRLLD
YQASLGCEQQALDRDATVALEPALAGIRQGIVGAIHTPSEEVGDCHRFCVSLAHLLQGRPGVTLRFGTRVTGLVRAGDRV
TGLRTDAGDIRADAVIVSGGIGSVPLLKPLGVRPMLWPLKGYSITVPIRQGVLAPRISVTDFANKIVYARIGDTLRVAGM
ADIVRGGVVIDRERAQTLVTQTRAVFGDVSHGADLDALQPWAGLRPATPTGLPMIGESRIRGLWLNLGHGALGFTLAMGS
AGVLADRMAGRKPVVDAEDFSAALA

Specific function: Oxidative deamination of D-amino acids [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=423, Percent_Identity=37.1158392434988, Blast_Score=273, Evalue=1e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023080
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.4.99.1 [H]

Molecular weight: Translated: 45296; Mature: 45296

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAIVGAGVVGMTSAWRLANDGHEVTVVERHDGPGEETSFANGGQLSYSYVAPLAGPGV
CEEEEEECCHHHHCCHHEECCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCH
LSKVPGWLLDRDSPMRFRPSMDPSQWRWLLAFARACNASASEAATRKLLRLSFYSRDLMQ
HHHCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AFVDRPDARQEGGGFDFARSGKLIVHRDAAAYESACRLLDYQASLGCEQQALDRDATVAL
HHHCCCCCCCCCCCCCEECCCCEEEEECHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEE
EPALAGIRQGIVGAIHTPSEEVGDCHRFCVSLAHLLQGRPGVTLRFGTRVTGLVRAGDRV
CHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCE
TGLRTDAGDIRADAVIVSGGIGSVPLLKPLGVRPMLWPLKGYSITVPIRQGVLAPRISVT
EEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEECCCCEEEEEHHCCCCCCCCCHH
DFANKIVYARIGDTLRVAGMADIVRGGVVIDRERAQTLVTQTRAVFGDVSHGADLDALQP
HHHCEEEEEECCCEEEEHHHHHHHHCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
WAGLRPATPTGLPMIGESRIRGLWLNLGHGALGFTLAMGSAGVLADRMAGRKPVVDAEDF
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHEEEEECCCCCHHHHHHCCCCCCCCHHHH
SAALA
HHHCC
>Mature Secondary Structure
MRIAIVGAGVVGMTSAWRLANDGHEVTVVERHDGPGEETSFANGGQLSYSYVAPLAGPGV
CEEEEEECCHHHHCCHHEECCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCH
LSKVPGWLLDRDSPMRFRPSMDPSQWRWLLAFARACNASASEAATRKLLRLSFYSRDLMQ
HHHCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AFVDRPDARQEGGGFDFARSGKLIVHRDAAAYESACRLLDYQASLGCEQQALDRDATVAL
HHHCCCCCCCCCCCCCEECCCCEEEEECHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEE
EPALAGIRQGIVGAIHTPSEEVGDCHRFCVSLAHLLQGRPGVTLRFGTRVTGLVRAGDRV
CHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCE
TGLRTDAGDIRADAVIVSGGIGSVPLLKPLGVRPMLWPLKGYSITVPIRQGVLAPRISVT
EEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEECCCCEEEEEHHCCCCCCCCCHH
DFANKIVYARIGDTLRVAGMADIVRGGVVIDRERAQTLVTQTRAVFGDVSHGADLDALQP
HHHCEEEEEECCCEEEEHHHHHHHHCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
WAGLRPATPTGLPMIGESRIRGLWLNLGHGALGFTLAMGSAGVLADRMAGRKPVVDAEDF
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHEEEEECCCCCHHHHHHCCCCCCCCHHHH
SAALA
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]