Click here to switch to the map view.

The map label for this gene is fabI [H]

Identifier: 94309510

GI number: 94309510

Start: 606409

End: 607188

Strand: Direct

Name: fabI [H]

Synonym: Rmet_0565

Alternate gene names: 94309510

Gene position: 606409-607188 (Clockwise)

Preceding gene: 94309509

Following gene: 94309511

Centisome position: 15.44

GC content: 66.41

Gene sequence:

>780_bases
ATGGTGCAAGTACCGAATCCACCCCTTGCAGGGGCGCGCGTGCTCGTCGTGGGCGTGGCGAACGAGGACTCGATTGCCTG
GGGCTGCGCCCGGGCGTTCCGGGAAATGGGCGCCGAGATCGCGCTGACCTATCTCAACGACAAGGCGCTACCGTACGTGG
AGCCACTGGCGCGCCAGGTGGAGGCGCCGATCCTGATGCCGCTCAATGTCGAGGACGATACACAGATGGACGCGCTATTC
GCGCGTATCGCAGCAGAATGGGGGCAGCTGGATTCCCTGGTCCATTCCGTGGCTTTCGCGCCGAAGGCCGACCTGCAGGG
CGGACTGCTCAATTCATCGGCCGCCGGATTCGCACGGGCGATGGATGTCTCCTGCCACTCGTTCATTCGCATGGCGCGCC
GCGCAGTGCCGCTGATGCCGCACGGCGGCACGATGTTCGCGATGAGCTACGACGGTGCCAACCGCGTGGTGCCGAACTAT
GACTTGATGGGGCCGGTAAAGGCCGCGCTGGAAGCCAGTTGCCGCTATCTGGCCTATGAGCTCGGGCCACGCAACATCCG
CGTGCACGCGATCTCGCCCGGGCCGCTGAAGACGCGCGCGGCGTCGGGGCTCAAGGACTTCGACGCTCTGCTGGCCGAGG
CGGCGGGGCGCGCGCCGTTGGGCGAACTGGTCGACATCATGGATGTTGGCTTCGCCACCGCCTACCTGGCTACGCCGTAC
GCGCGACGGCTGTCGGGCAACACCGTGTACGTCGATGGCGGTGTGCACGTGATGGCCTGA

Upstream 100 bases:

>100_bases
CAAGACCTGATTCACGATTACCGTTTTGCTCCGCCTCTGCGTGCCGGAGGCGGGGCGGTGGTCGTGTATCGCTCATCACG
TAGCTTCGCCGGAGAATCCG

Downstream 100 bases:

>100_bases
CAGGATGGGGCGACGCTGTGTGCGATCAGCGCATAAGGCACACGACGTGACGCGGAGCGCGGCCTACAATACACCCAATG
CCAACGCACCGAGAGAGAGG

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 2 [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MVQVPNPPLAGARVLVVGVANEDSIAWGCARAFREMGAEIALTYLNDKALPYVEPLARQVEAPILMPLNVEDDTQMDALF
ARIAAEWGQLDSLVHSVAFAPKADLQGGLLNSSAAGFARAMDVSCHSFIRMARRAVPLMPHGGTMFAMSYDGANRVVPNY
DLMGPVKAALEASCRYLAYELGPRNIRVHAISPGPLKTRAASGLKDFDALLAEAAGRAPLGELVDIMDVGFATAYLATPY
ARRLSGNTVYVDGGVHVMA

Sequences:

>Translated_259_residues
MVQVPNPPLAGARVLVVGVANEDSIAWGCARAFREMGAEIALTYLNDKALPYVEPLARQVEAPILMPLNVEDDTQMDALF
ARIAAEWGQLDSLVHSVAFAPKADLQGGLLNSSAAGFARAMDVSCHSFIRMARRAVPLMPHGGTMFAMSYDGANRVVPNY
DLMGPVKAALEASCRYLAYELGPRNIRVHAISPGPLKTRAASGLKDFDALLAEAAGRAPLGELVDIMDVGFATAYLATPY
ARRLSGNTVYVDGGVHVMA
>Mature_259_residues
MVQVPNPPLAGARVLVVGVANEDSIAWGCARAFREMGAEIALTYLNDKALPYVEPLARQVEAPILMPLNVEDDTQMDALF
ARIAAEWGQLDSLVHSVAFAPKADLQGGLLNSSAAGFARAMDVSCHSFIRMARRAVPLMPHGGTMFAMSYDGANRVVPNY
DLMGPVKAALEASCRYLAYELGPRNIRVHAISPGPLKTRAASGLKDFDALLAEAAGRAPLGELVDIMDVGFATAYLATPY
ARRLSGNTVYVDGGVHVMA

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI10190704, Length=241, Percent_Identity=26.9709543568465, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI32483357, Length=257, Percent_Identity=26.0700389105058, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787545, Length=252, Percent_Identity=42.4603174603175, Blast_Score=209, Evalue=1e-55,
Organism=Escherichia coli, GI1790717, Length=258, Percent_Identity=24.8062015503876, Blast_Score=64, Evalue=1e-11,
Organism=Drosophila melanogaster, GI23397609, Length=253, Percent_Identity=24.901185770751, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21357041, Length=261, Percent_Identity=25.2873563218391, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24644337, Length=243, Percent_Identity=27.5720164609054, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21355319, Length=257, Percent_Identity=24.9027237354086, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 27615; Mature: 27615

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQVPNPPLAGARVLVVGVANEDSIAWGCARAFREMGAEIALTYLNDKALPYVEPLARQV
CCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHC
EAPILMPLNVEDDTQMDALFARIAAEWGQLDSLVHSVAFAPKADLQGGLLNSSAAGFARA
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
MDVSCHSFIRMARRAVPLMPHGGTMFAMSYDGANRVVPNYDLMGPVKAALEASCRYLAYE
HHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHEEEE
LGPRNIRVHAISPGPLKTRAASGLKDFDALLAEAAGRAPLGELVDIMDVGFATAYLATPY
ECCCEEEEEEECCCCCHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCHH
ARRLSGNTVYVDGGVHVMA
HHHCCCCEEEECCCEEEEC
>Mature Secondary Structure
MVQVPNPPLAGARVLVVGVANEDSIAWGCARAFREMGAEIALTYLNDKALPYVEPLARQV
CCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHC
EAPILMPLNVEDDTQMDALFARIAAEWGQLDSLVHSVAFAPKADLQGGLLNSSAAGFARA
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
MDVSCHSFIRMARRAVPLMPHGGTMFAMSYDGANRVVPNYDLMGPVKAALEASCRYLAYE
HHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHEEEE
LGPRNIRVHAISPGPLKTRAASGLKDFDALLAEAAGRAPLGELVDIMDVGFATAYLATPY
ECCCEEEEEEECCCCCHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCHH
ARRLSGNTVYVDGGVHVMA
HHHCCCCEEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]